Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_565 and Pf1N1B4_566 are separated by 128 nucleotides Pf1N1B4_566 and Pf1N1B4_567 are separated by 83 nucleotides Pf1N1B4_567 and Pf1N1B4_568 are separated by 133 nucleotides Pf1N1B4_568 and Pf1N1B4_569 overlap by 4 nucleotides
Pf1N1B4_565 - Fatty acid desaturase, at 661,519 to 662,460
_565
Pf1N1B4_566 - hypothetical protein, at 662,589 to 662,717
_566
Pf1N1B4_567 - Histone acetyltransferase HPA2 and related acetyltransferases, at 662,801 to 663,220
_567
Pf1N1B4_568 - Peptide deformylase (EC 3.5.1.88), at 663,354 to 663,893
_568
Pf1N1B4_569 - Ribonuclease BN (EC 3.1.-.-), at 663,890 to 664,834
_569
Position (kb) 662
663
664 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 661.841 kb on + strand, within Pf1N1B4_565 at 661.911 kb on + strand, within Pf1N1B4_565 at 662.088 kb on - strand at 662.354 kb on + strand, within Pf1N1B4_565 at 662.354 kb on + strand, within Pf1N1B4_565 at 662.354 kb on + strand, within Pf1N1B4_565 at 662.354 kb on + strand, within Pf1N1B4_565 at 662.354 kb on + strand, within Pf1N1B4_565 at 662.355 kb on - strand, within Pf1N1B4_565 at 662.355 kb on - strand, within Pf1N1B4_565 at 662.355 kb on - strand, within Pf1N1B4_565 at 662.450 kb on + strand at 662.524 kb on - strand at 662.524 kb on - strand at 662.532 kb on - strand at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand at 662.702 kb on + strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand at 662.703 kb on - strand at 662.706 kb on + strand at 662.706 kb on + strand at 662.706 kb on + strand at 662.706 kb on + strand at 662.706 kb on + strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.717 kb on + strand at 662.726 kb on + strand at 662.726 kb on + strand at 662.726 kb on + strand at 662.726 kb on + strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.736 kb on + strand at 662.736 kb on + strand at 662.736 kb on + strand at 662.737 kb on - strand at 662.737 kb on - strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.744 kb on + strand at 662.745 kb on - strand at 662.745 kb on - strand at 662.766 kb on + strand at 662.767 kb on - strand at 662.767 kb on - strand at 662.768 kb on + strand at 662.768 kb on + strand at 662.769 kb on - strand at 662.769 kb on - strand at 662.769 kb on - strand at 662.816 kb on + strand at 662.816 kb on + strand at 662.816 kb on + strand at 662.817 kb on - strand at 662.817 kb on - strand at 662.943 kb on + strand at 662.943 kb on + strand, within Pf1N1B4_567 at 662.943 kb on + strand, within Pf1N1B4_567 at 662.943 kb on + strand, within Pf1N1B4_567 at 663.102 kb on - strand at 663.102 kb on - strand, within Pf1N1B4_567 at 663.102 kb on - strand, within Pf1N1B4_567 at 663.118 kb on + strand, within Pf1N1B4_567 at 663.119 kb on - strand, within Pf1N1B4_567 at 663.277 kb on - strand at 663.439 kb on - strand, within Pf1N1B4_568 at 664.060 kb on + strand, within Pf1N1B4_569 at 664.061 kb on - strand at 664.061 kb on - strand, within Pf1N1B4_569 at 664.062 kb on + strand, within Pf1N1B4_569 at 664.063 kb on - strand, within Pf1N1B4_569 at 664.063 kb on - strand, within Pf1N1B4_569 at 664.063 kb on - strand, within Pf1N1B4_569 at 664.063 kb on - strand at 664.063 kb on - strand, within Pf1N1B4_569 at 664.069 kb on - strand, within Pf1N1B4_569 at 664.104 kb on + strand, within Pf1N1B4_569 at 664.104 kb on + strand at 664.105 kb on - strand, within Pf1N1B4_569 at 664.105 kb on - strand, within Pf1N1B4_569 at 664.117 kb on - strand, within Pf1N1B4_569 at 664.117 kb on - strand at 664.137 kb on + strand, within Pf1N1B4_569 at 664.137 kb on + strand at 664.138 kb on - strand, within Pf1N1B4_569 at 664.202 kb on - strand, within Pf1N1B4_569
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 661,841 + Pf1N1B4_565 0.34 -0.2 661,911 + Pf1N1B4_565 0.42 -0.3 662,088 - +0.9 662,354 + Pf1N1B4_565 0.89 +0.9 662,354 + Pf1N1B4_565 0.89 -0.1 662,354 + Pf1N1B4_565 0.89 +0.9 662,354 + Pf1N1B4_565 0.89 +0.2 662,354 + Pf1N1B4_565 0.89 +0.4 662,355 - Pf1N1B4_565 0.89 -0.1 662,355 - Pf1N1B4_565 0.89 +0.1 662,355 - Pf1N1B4_565 0.89 +0.6 662,450 + -0.8 662,524 - -0.0 662,524 - -0.9 662,532 - +1.3 662,702 + Pf1N1B4_566 0.88 +1.3 662,702 + Pf1N1B4_566 0.88 -0.4 662,702 + Pf1N1B4_566 0.88 -0.5 662,702 + Pf1N1B4_566 0.88 +1.5 662,702 + Pf1N1B4_566 0.88 +0.5 662,702 + Pf1N1B4_566 0.88 +0.9 662,702 + Pf1N1B4_566 0.88 -0.7 662,702 + Pf1N1B4_566 0.88 +0.2 662,702 + Pf1N1B4_566 0.88 -1.5 662,702 + +0.9 662,702 + Pf1N1B4_566 0.88 +1.5 662,702 + Pf1N1B4_566 0.88 -1.7 662,702 + +0.9 662,702 + Pf1N1B4_566 0.88 +0.9 662,703 - Pf1N1B4_566 0.88 +1.1 662,703 - Pf1N1B4_566 0.88 -2.5 662,703 - Pf1N1B4_566 0.88 -0.9 662,703 - Pf1N1B4_566 0.88 -0.4 662,703 - Pf1N1B4_566 0.88 -0.9 662,703 - Pf1N1B4_566 0.88 +0.0 662,703 - Pf1N1B4_566 0.88 +0.3 662,703 - Pf1N1B4_566 0.88 -0.7 662,703 - Pf1N1B4_566 0.88 -0.5 662,703 - -1.1 662,703 - -0.7 662,706 + -1.4 662,706 + +0.9 662,706 + -0.8 662,706 + -0.3 662,706 + -1.0 662,707 - +0.6 662,707 - +0.3 662,707 - +0.9 662,707 - +0.0 662,707 - -0.1 662,707 - -0.4 662,707 - +1.9 662,707 - -0.3 662,707 - -1.7 662,717 + -0.7 662,726 + +1.3 662,726 + +1.4 662,726 + -0.7 662,726 + -0.1 662,727 - +2.1 662,727 - -0.7 662,727 - -2.0 662,727 - -0.9 662,727 - -1.1 662,727 - -1.5 662,727 - -0.7 662,727 - -0.7 662,727 - -1.7 662,727 - +1.9 662,736 + +0.2 662,736 + -1.9 662,736 + -1.4 662,737 - +0.3 662,737 - +1.5 662,738 + +0.4 662,738 + +0.7 662,738 + -0.8 662,738 + +0.3 662,738 + +0.9 662,738 + -1.1 662,738 + +2.5 662,738 + -0.7 662,738 + -0.2 662,738 + +2.5 662,739 - -0.7 662,739 - -1.5 662,739 - -1.1 662,739 - +0.4 662,739 - -1.4 662,739 - -0.4 662,739 - -0.9 662,739 - -0.1 662,739 - +0.1 662,739 - +0.3 662,739 - -1.4 662,739 - -2.1 662,739 - +0.6 662,739 - -1.9 662,739 - +1.7 662,739 - -1.0 662,739 - -1.1 662,739 - -0.2 662,739 - -0.9 662,739 - +0.1 662,739 - -0.7 662,739 - -1.1 662,739 - +0.4 662,744 + +0.6 662,745 - -0.9 662,745 - -1.1 662,766 + +0.9 662,767 - -2.9 662,767 - -1.2 662,768 + +0.9 662,768 + -2.4 662,769 - -1.7 662,769 - -0.7 662,769 - -1.5 662,816 + +0.9 662,816 + +0.3 662,816 + -0.8 662,817 - +0.3 662,817 - -0.7 662,943 + -0.7 662,943 + Pf1N1B4_567 0.34 +0.3 662,943 + Pf1N1B4_567 0.34 -0.7 662,943 + Pf1N1B4_567 0.34 -1.9 663,102 - +1.9 663,102 - Pf1N1B4_567 0.72 -1.5 663,102 - Pf1N1B4_567 0.72 -1.4 663,118 + Pf1N1B4_567 0.75 -0.3 663,119 - Pf1N1B4_567 0.76 -0.1 663,277 - +0.9 663,439 - Pf1N1B4_568 0.16 -0.5 664,060 + Pf1N1B4_569 0.18 +0.3 664,061 - -0.7 664,061 - Pf1N1B4_569 0.18 -0.1 664,062 + Pf1N1B4_569 0.18 +0.3 664,063 - Pf1N1B4_569 0.18 -0.1 664,063 - Pf1N1B4_569 0.18 -0.3 664,063 - Pf1N1B4_569 0.18 +0.2 664,063 - +0.9 664,063 - Pf1N1B4_569 0.18 -1.4 664,069 - Pf1N1B4_569 0.19 -1.7 664,104 + Pf1N1B4_569 0.23 -0.1 664,104 + -0.7 664,105 - Pf1N1B4_569 0.23 -1.9 664,105 - Pf1N1B4_569 0.23 -0.1 664,117 - Pf1N1B4_569 0.24 -0.7 664,117 - +1.9 664,137 + Pf1N1B4_569 0.26 +0.9 664,137 + -0.7 664,138 - Pf1N1B4_569 0.26 -2.1 664,202 - Pf1N1B4_569 0.33 +0.9
Or see this region's nucleotide sequence