Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5553 and Pf1N1B4_5554 are separated by 773 nucleotides Pf1N1B4_5554 and Pf1N1B4_5555 are separated by 172 nucleotides Pf1N1B4_5555 and Pf1N1B4_5556 are separated by 203 nucleotides
Pf1N1B4_5553 - Exodeoxyribonuclease I (EC 3.1.11.1), at 324,790 to 326,202
_5553
Pf1N1B4_5554 - hypothetical protein, at 326,976 to 327,200
_5554
Pf1N1B4_5555 - hypothetical protein, at 327,373 to 327,657
_5555
Pf1N1B4_5556 - hypothetical protein, at 327,861 to 328,058
_5556
Position (kb)
326
327
328 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 326.202 kb on + strand at 326.203 kb on - strand at 326.203 kb on - strand at 326.213 kb on - strand at 326.240 kb on - strand at 326.285 kb on - strand at 326.286 kb on + strand at 326.287 kb on - strand at 326.294 kb on + strand at 326.294 kb on + strand at 326.301 kb on - strand at 326.347 kb on - strand at 326.435 kb on - strand at 326.473 kb on - strand at 326.507 kb on - strand at 326.507 kb on - strand at 326.540 kb on + strand at 326.599 kb on + strand at 326.697 kb on - strand at 326.712 kb on - strand at 326.771 kb on + strand at 326.772 kb on - strand at 326.811 kb on - strand at 326.811 kb on - strand at 326.814 kb on + strand at 326.815 kb on - strand at 326.815 kb on - strand at 326.815 kb on - strand at 326.815 kb on - strand at 326.815 kb on - strand at 326.822 kb on + strand at 326.823 kb on - strand at 326.823 kb on - strand at 326.823 kb on - strand at 326.845 kb on + strand at 326.845 kb on + strand at 326.845 kb on + strand at 326.845 kb on + strand at 326.845 kb on + strand at 326.846 kb on - strand at 326.846 kb on - strand at 326.846 kb on - strand at 326.849 kb on + strand at 326.849 kb on + strand at 326.850 kb on - strand at 326.850 kb on - strand at 326.850 kb on - strand at 326.850 kb on - strand at 326.850 kb on - strand at 326.850 kb on - strand at 326.992 kb on + strand at 327.041 kb on - strand, within Pf1N1B4_5554 at 327.041 kb on - strand, within Pf1N1B4_5554 at 327.098 kb on - strand, within Pf1N1B4_5554 at 327.098 kb on - strand, within Pf1N1B4_5554 at 327.098 kb on - strand, within Pf1N1B4_5554 at 327.193 kb on - strand at 327.219 kb on - strand at 327.219 kb on - strand at 327.219 kb on - strand at 327.294 kb on - strand at 327.294 kb on - strand at 327.294 kb on - strand at 327.361 kb on - strand at 327.392 kb on + strand at 327.393 kb on - strand at 327.396 kb on - strand at 327.396 kb on - strand at 327.396 kb on - strand at 327.467 kb on + strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.473 kb on + strand, within Pf1N1B4_5555 at 327.474 kb on - strand, within Pf1N1B4_5555 at 327.474 kb on - strand at 327.530 kb on - strand, within Pf1N1B4_5555 at 327.580 kb on + strand, within Pf1N1B4_5555 at 327.581 kb on - strand, within Pf1N1B4_5555 at 327.581 kb on - strand, within Pf1N1B4_5555 at 327.581 kb on - strand, within Pf1N1B4_5555 at 327.601 kb on - strand, within Pf1N1B4_5555 at 327.601 kb on - strand, within Pf1N1B4_5555 at 327.608 kb on + strand, within Pf1N1B4_5555 at 327.609 kb on - strand, within Pf1N1B4_5555 at 327.805 kb on + strand at 327.806 kb on - strand at 327.888 kb on - strand, within Pf1N1B4_5556 at 327.952 kb on + strand, within Pf1N1B4_5556 at 327.953 kb on - strand, within Pf1N1B4_5556 at 327.953 kb on - strand at 327.953 kb on - strand, within Pf1N1B4_5556 at 327.993 kb on - strand, within Pf1N1B4_5556 at 328.144 kb on - strand at 328.193 kb on + strand at 328.193 kb on + strand at 328.193 kb on + strand at 328.193 kb on + strand at 328.194 kb on - strand at 328.194 kb on - strand at 328.194 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 326,202 + +1.1 326,203 - -0.9 326,203 - +2.1 326,213 - -0.3 326,240 - +1.1 326,285 - -0.7 326,286 + +1.1 326,287 - -0.5 326,294 + +1.6 326,294 + -0.5 326,301 - +0.1 326,347 - +0.6 326,435 - +0.1 326,473 - +0.7 326,507 - -0.7 326,507 - -0.2 326,540 + +0.1 326,599 + +2.4 326,697 - +0.5 326,712 - -0.7 326,771 + -0.8 326,772 - -1.5 326,811 - +1.5 326,811 - -0.5 326,814 + +0.3 326,815 - +0.1 326,815 - -0.4 326,815 - -0.7 326,815 - -1.1 326,815 - +1.4 326,822 + -0.9 326,823 - +0.1 326,823 - -0.1 326,823 - +1.1 326,845 + +0.1 326,845 + +1.8 326,845 + +0.1 326,845 + +1.1 326,845 + +1.1 326,846 - -1.2 326,846 - -1.3 326,846 - -0.5 326,849 + -0.2 326,849 + -1.7 326,850 - -0.6 326,850 - -0.3 326,850 - +0.3 326,850 - +1.9 326,850 - -0.9 326,850 - +0.1 326,992 + -0.7 327,041 - Pf1N1B4_5554 0.29 +2.6 327,041 - Pf1N1B4_5554 0.29 -1.4 327,098 - Pf1N1B4_5554 0.54 -1.7 327,098 - Pf1N1B4_5554 0.54 -1.3 327,098 - Pf1N1B4_5554 0.54 -1.0 327,193 - -0.4 327,219 - +0.1 327,219 - -2.1 327,219 - +1.1 327,294 - +1.1 327,294 - -0.1 327,294 - -0.3 327,361 - +1.1 327,392 + -1.5 327,393 - +0.3 327,396 - -0.3 327,396 - +1.3 327,396 - -1.7 327,467 + Pf1N1B4_5555 0.33 +0.7 327,468 - Pf1N1B4_5555 0.33 -0.3 327,468 - Pf1N1B4_5555 0.33 -0.1 327,468 - Pf1N1B4_5555 0.33 +0.3 327,468 - Pf1N1B4_5555 0.33 +0.3 327,468 - Pf1N1B4_5555 0.33 -0.7 327,468 - Pf1N1B4_5555 0.33 -0.5 327,473 + Pf1N1B4_5555 0.35 +1.6 327,474 - Pf1N1B4_5555 0.35 +1.1 327,474 - +1.6 327,530 - Pf1N1B4_5555 0.55 -0.5 327,580 + Pf1N1B4_5555 0.73 +1.1 327,581 - Pf1N1B4_5555 0.73 -0.9 327,581 - Pf1N1B4_5555 0.73 -1.3 327,581 - Pf1N1B4_5555 0.73 -1.1 327,601 - Pf1N1B4_5555 0.80 -0.0 327,601 - Pf1N1B4_5555 0.80 +0.5 327,608 + Pf1N1B4_5555 0.82 +0.3 327,609 - Pf1N1B4_5555 0.83 +0.1 327,805 + +0.6 327,806 - -1.5 327,888 - Pf1N1B4_5556 0.14 -0.4 327,952 + Pf1N1B4_5556 0.46 +0.6 327,953 - Pf1N1B4_5556 0.46 +0.1 327,953 - +0.1 327,953 - Pf1N1B4_5556 0.46 +1.1 327,993 - Pf1N1B4_5556 0.67 +0.1 328,144 - -0.7 328,193 + -2.3 328,193 + +1.6 328,193 + -0.5 328,193 + -0.1 328,194 - +0.5 328,194 - -1.1 328,194 - -0.1
Or see this region's nucleotide sequence