Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5448

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5448 and Pf1N1B4_5449 are separated by 312 nucleotidesPf1N1B4_5449 and Pf1N1B4_5450 overlap by 25 nucleotides Pf1N1B4_5448 - hypothetical protein, at 194,385 to 194,507 _5448 Pf1N1B4_5449 - Error-prone repair protein UmuD, at 194,820 to 195,131 _5449 Pf1N1B4_5450 - Error-prone, lesion bypass DNA polymerase V (UmuC), at 195,107 to 196,402 _5450 Position (kb) 194 195Strain fitness (log2 ratio) -2 -1 0 1 2 3at 193.399 kb on + strandat 193.449 kb on + strandat 193.450 kb on - strandat 193.552 kb on - strandat 193.552 kb on - strandat 193.836 kb on + strandat 193.836 kb on + strandat 193.837 kb on - strandat 193.837 kb on - strandat 193.873 kb on - strandat 193.879 kb on + strandat 193.880 kb on - strandat 193.880 kb on - strandat 193.889 kb on - strandat 193.889 kb on - strandat 193.889 kb on - strandat 194.054 kb on + strandat 194.055 kb on - strandat 194.055 kb on - strandat 194.176 kb on + strandat 194.176 kb on + strandat 194.177 kb on - strandat 194.177 kb on - strandat 194.177 kb on - strandat 194.177 kb on - strandat 194.278 kb on - strandat 194.278 kb on - strandat 194.328 kb on + strandat 194.328 kb on + strandat 194.329 kb on - strandat 194.440 kb on + strand, within Pf1N1B4_5448at 194.441 kb on - strand, within Pf1N1B4_5448at 194.441 kb on - strand, within Pf1N1B4_5448at 194.441 kb on - strand, within Pf1N1B4_5448at 194.441 kb on - strandat 194.441 kb on - strand, within Pf1N1B4_5448at 194.495 kb on + strandat 194.496 kb on - strandat 194.543 kb on - strandat 194.543 kb on - strandat 194.543 kb on - strandat 194.601 kb on + strandat 194.602 kb on - strandat 194.672 kb on - strandat 194.709 kb on - strandat 194.709 kb on - strandat 194.730 kb on + strandat 194.731 kb on - strandat 194.731 kb on - strandat 194.731 kb on - strandat 194.749 kb on + strandat 194.750 kb on - strandat 194.798 kb on + strandat 194.798 kb on + strandat 194.799 kb on - strandat 194.799 kb on - strandat 195.050 kb on + strand, within Pf1N1B4_5449at 195.051 kb on - strand, within Pf1N1B4_5449at 195.173 kb on + strandat 195.174 kb on - strandat 195.282 kb on + strand, within Pf1N1B4_5450at 195.282 kb on + strand, within Pf1N1B4_5450at 195.345 kb on + strandat 195.345 kb on + strand, within Pf1N1B4_5450at 195.345 kb on + strand, within Pf1N1B4_5450at 195.345 kb on + strand, within Pf1N1B4_5450at 195.346 kb on - strand, within Pf1N1B4_5450at 195.346 kb on - strand, within Pf1N1B4_5450at 195.346 kb on - strand, within Pf1N1B4_5450at 195.349 kb on - strand, within Pf1N1B4_5450at 195.349 kb on - strand, within Pf1N1B4_5450at 195.379 kb on + strand, within Pf1N1B4_5450at 195.441 kb on + strand, within Pf1N1B4_5450at 195.441 kb on + strand, within Pf1N1B4_5450at 195.441 kb on + strand, within Pf1N1B4_5450at 195.442 kb on - strand, within Pf1N1B4_5450at 195.442 kb on - strand, within Pf1N1B4_5450at 195.456 kb on + strand, within Pf1N1B4_5450at 195.456 kb on + strand, within Pf1N1B4_5450at 195.456 kb on + strand, within Pf1N1B4_5450at 195.456 kb on + strand, within Pf1N1B4_5450at 195.456 kb on + strand, within Pf1N1B4_5450at 195.457 kb on - strand, within Pf1N1B4_5450at 195.457 kb on - strand, within Pf1N1B4_5450at 195.457 kb on - strand, within Pf1N1B4_5450

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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193,399 + +0.1
193,449 + -0.5
193,450 - +0.1
193,552 - -0.3
193,552 - +0.7
193,836 + -0.9
193,836 + -1.3
193,837 - +0.6
193,837 - -0.1
193,873 - -0.9
193,879 + -0.1
193,880 - -0.5
193,880 - +2.6
193,889 - -0.3
193,889 - +0.1
193,889 - +0.6
194,054 + -0.7
194,055 - -0.8
194,055 - +1.1
194,176 + +0.3
194,176 + -0.4
194,177 - +0.3
194,177 - +0.6
194,177 - +0.2
194,177 - -0.4
194,278 - +0.1
194,278 - -0.4
194,328 + -0.6
194,328 + -0.6
194,329 - +2.4
194,440 + Pf1N1B4_5448 0.45 -0.3
194,441 - Pf1N1B4_5448 0.46 -1.3
194,441 - Pf1N1B4_5448 0.46 +0.2
194,441 - Pf1N1B4_5448 0.46 -0.9
194,441 - +2.1
194,441 - Pf1N1B4_5448 0.46 -1.8
194,495 + +3.4
194,496 - +0.6
194,543 - -0.2
194,543 - +0.1
194,543 - -0.4
194,601 + -1.3
194,602 - -0.5
194,672 - +0.5
194,709 - -0.2
194,709 - +0.6
194,730 + -0.3
194,731 - +0.1
194,731 - -1.3
194,731 - -0.8
194,749 + -1.0
194,750 - -2.1
194,798 + +0.6
194,798 + +0.9
194,799 - -1.3
194,799 - -1.1
195,050 + Pf1N1B4_5449 0.74 -2.5
195,051 - Pf1N1B4_5449 0.74 +1.9
195,173 + -2.3
195,174 - -0.3
195,282 + Pf1N1B4_5450 0.14 +0.1
195,282 + Pf1N1B4_5450 0.14 -0.4
195,345 + +1.1
195,345 + Pf1N1B4_5450 0.18 -0.9
195,345 + Pf1N1B4_5450 0.18 +0.3
195,345 + Pf1N1B4_5450 0.18 +0.6
195,346 - Pf1N1B4_5450 0.18 -1.4
195,346 - Pf1N1B4_5450 0.18 +0.3
195,346 - Pf1N1B4_5450 0.18 -0.7
195,349 - Pf1N1B4_5450 0.19 +0.9
195,349 - Pf1N1B4_5450 0.19 -0.5
195,379 + Pf1N1B4_5450 0.21 +1.5
195,441 + Pf1N1B4_5450 0.26 -1.5
195,441 + Pf1N1B4_5450 0.26 -1.1
195,441 + Pf1N1B4_5450 0.26 -0.9
195,442 - Pf1N1B4_5450 0.26 -2.1
195,442 - Pf1N1B4_5450 0.26 +0.5
195,456 + Pf1N1B4_5450 0.27 -0.3
195,456 + Pf1N1B4_5450 0.27 -0.7
195,456 + Pf1N1B4_5450 0.27 +0.1
195,456 + Pf1N1B4_5450 0.27 -0.3
195,456 + Pf1N1B4_5450 0.27 -0.5
195,457 - Pf1N1B4_5450 0.27 +1.9
195,457 - Pf1N1B4_5450 0.27 -0.9
195,457 - Pf1N1B4_5450 0.27 +0.1

Or see this region's nucleotide sequence