Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_51 and Pf1N1B4_52 are separated by 337 nucleotides Pf1N1B4_52 and Pf1N1B4_53 are separated by 6 nucleotides Pf1N1B4_53 and Pf1N1B4_54 are separated by 194 nucleotides
Pf1N1B4_51 - hypothetical protein, at 57,079 to 57,348
_51
Pf1N1B4_52 - FIG00956056: hypothetical protein, at 57,686 to 58,144
_52
Pf1N1B4_53 - hypothetical protein, at 58,151 to 58,342
_53
Pf1N1B4_54 - Cyclohexadienyl dehydratase (EC 4.2.1.51)(EC 4.2.1.91), at 58,537 to 59,328
_54
Position (kb) 58
59 Strain fitness (log2 ratio) -2
-1
0
1
2 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.222 kb on - strand, within Pf1N1B4_51 at 57.222 kb on - strand, within Pf1N1B4_51 at 57.409 kb on + strand at 57.432 kb on - strand at 57.504 kb on - strand at 57.512 kb on + strand at 57.512 kb on + strand at 57.513 kb on - strand at 57.603 kb on - strand at 57.603 kb on - strand at 57.717 kb on - strand at 57.717 kb on - strand at 57.875 kb on - strand, within Pf1N1B4_52 at 58.055 kb on - strand, within Pf1N1B4_52 at 58.072 kb on + strand, within Pf1N1B4_52 at 58.093 kb on + strand, within Pf1N1B4_52 at 58.142 kb on + strand at 58.182 kb on + strand, within Pf1N1B4_53 at 58.186 kb on + strand, within Pf1N1B4_53 at 58.202 kb on + strand, within Pf1N1B4_53 at 58.202 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.207 kb on - strand, within Pf1N1B4_53 at 58.272 kb on + strand, within Pf1N1B4_53 at 58.352 kb on + strand at 58.388 kb on + strand at 58.389 kb on - strand at 58.389 kb on - strand at 58.426 kb on + strand at 58.427 kb on - strand at 58.427 kb on - strand at 58.548 kb on - strand at 58.684 kb on - strand, within Pf1N1B4_54 at 58.684 kb on - strand, within Pf1N1B4_54 at 58.684 kb on - strand, within Pf1N1B4_54 at 58.709 kb on - strand, within Pf1N1B4_54 at 59.132 kb on - strand at 59.145 kb on + strand, within Pf1N1B4_54 at 59.181 kb on + strand, within Pf1N1B4_54 at 59.182 kb on - strand, within Pf1N1B4_54 at 59.189 kb on - strand, within Pf1N1B4_54 at 59.203 kb on + strand, within Pf1N1B4_54 at 59.203 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.282 kb on - strand at 59.282 kb on - strand at 59.322 kb on + strand at 59.322 kb on + strand at 59.326 kb on + strand at 59.326 kb on + strand at 59.326 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 57,221 + Pf1N1B4_51 0.53 -0.7 57,221 + Pf1N1B4_51 0.53 -0.6 57,221 + -0.3 57,221 + Pf1N1B4_51 0.53 +0.6 57,221 + Pf1N1B4_51 0.53 +1.3 57,221 + Pf1N1B4_51 0.53 +0.3 57,221 + Pf1N1B4_51 0.53 +1.4 57,222 - Pf1N1B4_51 0.53 +0.7 57,222 - Pf1N1B4_51 0.53 -2.0 57,409 + +0.7 57,432 - -1.3 57,504 - +0.2 57,512 + -2.3 57,512 + +0.5 57,513 - +0.5 57,603 - +0.7 57,603 - +0.1 57,717 - -0.3 57,717 - +0.1 57,875 - Pf1N1B4_52 0.41 -0.3 58,055 - Pf1N1B4_52 0.80 +0.7 58,072 + Pf1N1B4_52 0.84 +0.7 58,093 + Pf1N1B4_52 0.89 -0.3 58,142 + -0.3 58,182 + Pf1N1B4_53 0.16 -2.1 58,186 + Pf1N1B4_53 0.18 -1.1 58,202 + Pf1N1B4_53 0.27 +0.4 58,202 + Pf1N1B4_53 0.27 -0.3 58,206 + +1.3 58,206 + Pf1N1B4_53 0.29 -0.9 58,206 + Pf1N1B4_53 0.29 -1.7 58,206 + Pf1N1B4_53 0.29 -1.0 58,206 + Pf1N1B4_53 0.29 -2.1 58,207 - Pf1N1B4_53 0.29 -1.1 58,272 + Pf1N1B4_53 0.63 -0.6 58,352 + +0.7 58,388 + -2.7 58,389 - +2.0 58,389 - +0.7 58,426 + -0.0 58,427 - +0.5 58,427 - -0.9 58,548 - -0.0 58,684 - Pf1N1B4_54 0.19 -1.1 58,684 - Pf1N1B4_54 0.19 +0.0 58,684 - Pf1N1B4_54 0.19 -0.3 58,709 - Pf1N1B4_54 0.22 -0.4 59,132 - +0.7 59,145 + Pf1N1B4_54 0.77 +1.3 59,181 + Pf1N1B4_54 0.81 -0.9 59,182 - Pf1N1B4_54 0.81 +0.1 59,189 - Pf1N1B4_54 0.82 -0.0 59,203 + Pf1N1B4_54 0.84 -0.6 59,203 + Pf1N1B4_54 0.84 -0.9 59,239 + Pf1N1B4_54 0.89 +1.0 59,239 + Pf1N1B4_54 0.89 -0.1 59,239 + Pf1N1B4_54 0.89 -2.4 59,239 + Pf1N1B4_54 0.89 -0.7 59,239 + +0.1 59,239 + Pf1N1B4_54 0.89 -0.1 59,239 + Pf1N1B4_54 0.89 +0.3 59,239 + Pf1N1B4_54 0.89 -0.9 59,282 - -1.1 59,282 - +0.7 59,322 + -2.1 59,322 + -0.5 59,326 + +0.7 59,326 + -0.0 59,326 + +0.7
Or see this region's nucleotide sequence