Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5250 and Pf1N1B4_5251 are separated by 522 nucleotides Pf1N1B4_5251 and Pf1N1B4_5252 are separated by 169 nucleotides Pf1N1B4_5252 and Pf1N1B4_5253 are separated by 11 nucleotides
Pf1N1B4_5250 - hypothetical protein, at 8,866 to 8,994
_5250
Pf1N1B4_5251 - hypothetical protein, at 9,517 to 10,368
_5251
Pf1N1B4_5252 - hypothetical protein, at 10,538 to 11,272
_5252
Pf1N1B4_5253 - hypothetical protein, at 11,284 to 12,045
_5253
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 8.594 kb on + strand at 8.595 kb on - strand at 8.595 kb on - strand at 8.642 kb on + strand at 8.643 kb on - strand at 8.654 kb on + strand at 8.655 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.676 kb on - strand at 8.761 kb on + strand at 8.761 kb on + strand at 8.814 kb on + strand at 8.818 kb on + strand at 8.819 kb on - strand at 8.819 kb on - strand at 9.008 kb on - strand at 9.074 kb on + strand at 9.074 kb on + strand at 9.075 kb on - strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.410 kb on + strand at 9.410 kb on + strand at 9.411 kb on - strand at 9.411 kb on - strand at 9.417 kb on + strand at 9.417 kb on + strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.422 kb on - strand at 9.422 kb on - strand at 9.518 kb on + strand at 9.518 kb on + strand at 9.519 kb on - strand at 9.558 kb on + strand at 9.558 kb on + strand at 9.596 kb on + strand at 9.597 kb on - strand at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.712 kb on + strand, within Pf1N1B4_5251 at 9.920 kb on + strand at 9.920 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.137 kb on - strand, within Pf1N1B4_5251 at 10.137 kb on - strand, within Pf1N1B4_5251 at 10.137 kb on - strand at 10.137 kb on - strand at 10.357 kb on - strand at 10.384 kb on + strand at 10.401 kb on - strand at 10.401 kb on - strand at 10.453 kb on - strand at 10.453 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.552 kb on - strand at 10.733 kb on + strand, within Pf1N1B4_5252 at 10.780 kb on - strand, within Pf1N1B4_5252 at 10.803 kb on + strand, within Pf1N1B4_5252 at 10.803 kb on + strand, within Pf1N1B4_5252 at 10.819 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.841 kb on - strand, within Pf1N1B4_5252 at 10.929 kb on + strand, within Pf1N1B4_5252 at 11.130 kb on + strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.134 kb on + strand, within Pf1N1B4_5252 at 11.134 kb on + strand, within Pf1N1B4_5252 at 11.159 kb on + strand, within Pf1N1B4_5252 at 11.205 kb on + strand at 11.206 kb on - strand at 11.206 kb on - strand at 11.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 8,594 + -0.2 8,595 - -0.5 8,595 - +0.3 8,642 + +0.1 8,643 - -1.2 8,654 + +0.1 8,655 - -2.5 8,659 - -0.5 8,659 - -0.2 8,659 - -0.5 8,659 - +1.7 8,659 - -0.5 8,676 - -0.9 8,761 + +0.8 8,761 + -1.2 8,814 + +0.7 8,818 + -0.9 8,819 - -1.5 8,819 - +2.3 9,008 - -0.5 9,074 + -1.4 9,074 + +0.1 9,075 - +0.2 9,406 + +1.1 9,406 + +0.7 9,406 + +0.5 9,406 + +0.5 9,406 + -0.1 9,407 - +0.1 9,407 - -0.7 9,407 - +1.1 9,407 - +0.7 9,410 + -2.1 9,410 + -0.2 9,411 - +1.1 9,411 - +0.1 9,417 + +0.5 9,417 + -0.9 9,418 - +0.2 9,418 - -1.1 9,418 - -1.7 9,418 - -0.2 9,418 - +1.4 9,418 - +0.5 9,418 - +0.3 9,418 - -0.6 9,418 - -1.2 9,418 - -0.5 9,418 - -0.2 9,422 - -0.8 9,422 - -0.8 9,518 + +0.3 9,518 + -0.8 9,519 - -0.2 9,558 + -1.5 9,558 + -0.2 9,596 + -0.3 9,597 - +2.1 9,640 - Pf1N1B4_5251 0.14 -0.5 9,640 - Pf1N1B4_5251 0.14 +0.6 9,640 - Pf1N1B4_5251 0.14 +0.3 9,640 - Pf1N1B4_5251 0.14 +0.8 9,640 - Pf1N1B4_5251 0.14 -0.9 9,640 - Pf1N1B4_5251 0.14 -1.6 9,712 + Pf1N1B4_5251 0.23 -0.8 9,920 + -0.5 9,920 + Pf1N1B4_5251 0.47 +0.1 10,136 + Pf1N1B4_5251 0.73 +0.1 10,136 + Pf1N1B4_5251 0.73 -0.6 10,136 + Pf1N1B4_5251 0.73 +0.1 10,136 + Pf1N1B4_5251 0.73 +0.2 10,136 + Pf1N1B4_5251 0.73 -1.4 10,136 + Pf1N1B4_5251 0.73 +0.5 10,136 + Pf1N1B4_5251 0.73 +1.1 10,136 + Pf1N1B4_5251 0.73 -0.9 10,137 - Pf1N1B4_5251 0.73 +0.1 10,137 - Pf1N1B4_5251 0.73 -1.3 10,137 - -0.5 10,137 - +1.1 10,357 - -0.1 10,384 + -1.0 10,401 - +1.1 10,401 - +1.6 10,453 - +0.7 10,453 - -0.3 10,496 - -1.0 10,496 - +3.7 10,496 - +0.5 10,496 - -1.2 10,500 - -0.2 10,500 - -0.2 10,500 - +0.6 10,500 - -0.1 10,552 - +0.7 10,733 + Pf1N1B4_5252 0.27 -1.4 10,780 - Pf1N1B4_5252 0.33 -0.0 10,803 + Pf1N1B4_5252 0.36 -0.9 10,803 + Pf1N1B4_5252 0.36 +3.1 10,819 + Pf1N1B4_5252 0.38 -0.8 10,821 + Pf1N1B4_5252 0.39 +1.9 10,821 + Pf1N1B4_5252 0.39 -0.9 10,821 + Pf1N1B4_5252 0.39 +0.4 10,822 - Pf1N1B4_5252 0.39 +1.8 10,822 - Pf1N1B4_5252 0.39 +0.8 10,822 - Pf1N1B4_5252 0.39 -1.3 10,841 - Pf1N1B4_5252 0.41 -0.9 10,929 + Pf1N1B4_5252 0.53 +1.7 11,130 + Pf1N1B4_5252 0.81 +1.3 11,131 - Pf1N1B4_5252 0.81 +0.6 11,131 - Pf1N1B4_5252 0.81 -2.4 11,131 - Pf1N1B4_5252 0.81 +0.7 11,134 + Pf1N1B4_5252 0.81 +0.1 11,134 + Pf1N1B4_5252 0.81 -0.3 11,159 + Pf1N1B4_5252 0.84 -0.3 11,205 + -0.3 11,206 - +1.1 11,206 - -1.2 11,206 - +0.1
Or see this region's nucleotide sequence