Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5115

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5113 and Pf1N1B4_5114 overlap by 4 nucleotidesPf1N1B4_5114 and Pf1N1B4_5115 are separated by 27 nucleotidesPf1N1B4_5115 and Pf1N1B4_5116 are separated by 29 nucleotides Pf1N1B4_5113 - sucrose ABC transporter, permease component 1 (from data), at 5,672,987 to 5,673,874 _5113 Pf1N1B4_5114 - sucrose ABC transporter, permease component 2 (from data), at 5,673,871 to 5,674,713 _5114 Pf1N1B4_5115 - sucrose ABC transporter, ATPase component (from data), at 5,674,741 to 5,675,886 _5115 Pf1N1B4_5116 - Sucrose alpha-glucosidase (EC 3.2.1.48) (from data), at 5,675,916 to 5,677,385 _5116 Position (kb) 5674 5675 5676Strain fitness (log2 ratio) -2 -1 0 1 2at 5674.045 kb on + strand, within Pf1N1B4_5114at 5674.476 kb on - strand, within Pf1N1B4_5114at 5674.792 kb on - strandat 5674.999 kb on + strand, within Pf1N1B4_5115at 5675.305 kb on + strand, within Pf1N1B4_5115at 5675.306 kb on - strand, within Pf1N1B4_5115at 5675.404 kb on + strand, within Pf1N1B4_5115at 5675.404 kb on + strandat 5675.405 kb on - strand, within Pf1N1B4_5115at 5675.654 kb on - strand, within Pf1N1B4_5115at 5675.671 kb on + strand, within Pf1N1B4_5115at 5675.671 kb on + strand, within Pf1N1B4_5115at 5675.671 kb on + strandat 5675.672 kb on - strand, within Pf1N1B4_5115at 5675.883 kb on - strandat 5676.046 kb on - strandat 5676.073 kb on - strand, within Pf1N1B4_5116at 5676.073 kb on - strandat 5676.111 kb on + strand, within Pf1N1B4_5116at 5676.249 kb on + strand, within Pf1N1B4_5116at 5676.250 kb on - strand, within Pf1N1B4_5116at 5676.409 kb on + strand, within Pf1N1B4_5116at 5676.433 kb on - strand, within Pf1N1B4_5116at 5676.501 kb on - strand, within Pf1N1B4_5116at 5676.501 kb on - strand, within Pf1N1B4_5116at 5676.519 kb on + strand, within Pf1N1B4_5116at 5676.549 kb on + strandat 5676.550 kb on - strand, within Pf1N1B4_5116at 5676.551 kb on + strand, within Pf1N1B4_5116at 5676.551 kb on + strand, within Pf1N1B4_5116at 5676.551 kb on + strand, within Pf1N1B4_5116at 5676.551 kb on + strand, within Pf1N1B4_5116at 5676.551 kb on + strand, within Pf1N1B4_5116at 5676.551 kb on + strand, within Pf1N1B4_5116at 5676.551 kb on + strand, within Pf1N1B4_5116at 5676.552 kb on - strand, within Pf1N1B4_5116at 5676.595 kb on + strand, within Pf1N1B4_5116at 5676.596 kb on - strand, within Pf1N1B4_5116at 5676.596 kb on - strandat 5676.596 kb on - strand, within Pf1N1B4_5116at 5676.596 kb on - strand, within Pf1N1B4_5116at 5676.603 kb on + strand, within Pf1N1B4_5116at 5676.604 kb on - strand, within Pf1N1B4_5116at 5676.652 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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5,674,045 + Pf1N1B4_5114 0.21 +0.3
5,674,476 - Pf1N1B4_5114 0.72 -0.7
5,674,792 - +1.1
5,674,999 + Pf1N1B4_5115 0.23 +0.9
5,675,305 + Pf1N1B4_5115 0.49 +1.6
5,675,306 - Pf1N1B4_5115 0.49 -0.1
5,675,404 + Pf1N1B4_5115 0.58 -0.3
5,675,404 + +0.9
5,675,405 - Pf1N1B4_5115 0.58 +2.1
5,675,654 - Pf1N1B4_5115 0.80 +0.2
5,675,671 + Pf1N1B4_5115 0.81 -1.7
5,675,671 + Pf1N1B4_5115 0.81 +1.0
5,675,671 + +0.3
5,675,672 - Pf1N1B4_5115 0.81 -1.7
5,675,883 - -1.1
5,676,046 - -0.7
5,676,073 - Pf1N1B4_5116 0.11 +0.8
5,676,073 - +0.3
5,676,111 + Pf1N1B4_5116 0.13 -1.0
5,676,249 + Pf1N1B4_5116 0.23 -0.3
5,676,250 - Pf1N1B4_5116 0.23 -1.0
5,676,409 + Pf1N1B4_5116 0.34 -0.1
5,676,433 - Pf1N1B4_5116 0.35 +0.1
5,676,501 - Pf1N1B4_5116 0.40 -0.3
5,676,501 - Pf1N1B4_5116 0.40 -0.4
5,676,519 + Pf1N1B4_5116 0.41 -0.5
5,676,549 + +0.3
5,676,550 - Pf1N1B4_5116 0.43 +0.3
5,676,551 + Pf1N1B4_5116 0.43 +0.1
5,676,551 + Pf1N1B4_5116 0.43 -2.0
5,676,551 + Pf1N1B4_5116 0.43 -0.4
5,676,551 + Pf1N1B4_5116 0.43 -0.7
5,676,551 + Pf1N1B4_5116 0.43 +0.3
5,676,551 + Pf1N1B4_5116 0.43 -0.7
5,676,551 + Pf1N1B4_5116 0.43 -0.5
5,676,552 - Pf1N1B4_5116 0.43 +0.6
5,676,595 + Pf1N1B4_5116 0.46 +1.1
5,676,596 - Pf1N1B4_5116 0.46 -1.3
5,676,596 - -1.3
5,676,596 - Pf1N1B4_5116 0.46 -1.9
5,676,596 - Pf1N1B4_5116 0.46 +0.1
5,676,603 + Pf1N1B4_5116 0.47 -0.9
5,676,604 - Pf1N1B4_5116 0.47 -0.1
5,676,652 - -0.2

Or see this region's nucleotide sequence