Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5094

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5094 and Pf1N1B4_5095 are separated by 80 nucleotides Pf1N1B4_5094 - Methyl-accepting chemotaxis protein, at 5,647,912 to 5,648,736 _5094 Pf1N1B4_5095 - hypothetical protein, at 5,648,817 to 5,649,380 _5095 Position (kb) 5647 5648 5649Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 5647.153 kb on + strandat 5647.153 kb on + strandat 5647.326 kb on + strandat 5647.327 kb on - strandat 5647.329 kb on - strandat 5647.409 kb on + strandat 5647.410 kb on - strandat 5647.531 kb on + strandat 5647.767 kb on - strandat 5647.826 kb on + strandat 5647.827 kb on - strandat 5647.827 kb on - strandat 5647.833 kb on - strandat 5647.949 kb on + strandat 5648.236 kb on - strand, within Pf1N1B4_5094at 5648.547 kb on + strand, within Pf1N1B4_5094at 5648.548 kb on - strand, within Pf1N1B4_5094at 5648.548 kb on - strand, within Pf1N1B4_5094at 5648.667 kb on + strandat 5648.741 kb on - strandat 5648.952 kb on + strand, within Pf1N1B4_5095at 5648.984 kb on - strand, within Pf1N1B4_5095at 5649.200 kb on - strand, within Pf1N1B4_5095at 5649.200 kb on - strand, within Pf1N1B4_5095at 5649.205 kb on - strand, within Pf1N1B4_5095at 5649.385 kb on + strandat 5649.385 kb on + strandat 5649.385 kb on + strandat 5649.386 kb on - strandat 5649.386 kb on - strandat 5649.427 kb on + strandat 5649.487 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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5,647,153 + +0.3
5,647,153 + +0.9
5,647,326 + -0.5
5,647,327 - -0.2
5,647,329 - -2.0
5,647,409 + +0.7
5,647,410 - -2.0
5,647,531 + -2.0
5,647,767 - -1.5
5,647,826 + -0.1
5,647,827 - -0.7
5,647,827 - -1.3
5,647,833 - -0.3
5,647,949 + -0.7
5,648,236 - Pf1N1B4_5094 0.39 -0.7
5,648,547 + Pf1N1B4_5094 0.77 -0.7
5,648,548 - Pf1N1B4_5094 0.77 -1.4
5,648,548 - Pf1N1B4_5094 0.77 +0.9
5,648,667 + -0.1
5,648,741 - +3.3
5,648,952 + Pf1N1B4_5095 0.24 -0.2
5,648,984 - Pf1N1B4_5095 0.30 -3.1
5,649,200 - Pf1N1B4_5095 0.68 -1.4
5,649,200 - Pf1N1B4_5095 0.68 -2.5
5,649,205 - Pf1N1B4_5095 0.69 -1.1
5,649,385 + -0.9
5,649,385 + +0.2
5,649,385 + -1.3
5,649,386 - -0.2
5,649,386 - -2.3
5,649,427 + +0.2
5,649,487 + -0.9

Or see this region's nucleotide sequence