Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5087

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPf1N1B4_5085 and Pf1N1B4_5086 are separated by 29 nucleotidesPf1N1B4_5086 and Pf1N1B4_5087 are separated by 23 nucleotidesPf1N1B4_5087 and Pf1N1B4_5088 overlap by 4 nucleotides Pf1N1B4_5085 - Capsular polysaccharide synthesis enzyme CpsA, sugar transferase, at 5,635,163 to 5,636,554 _5085 Pf1N1B4_5086 - N-acetylmannosaminyltransferase (EC 2.4.1.187), at 5,636,584 to 5,637,291 _5086 Pf1N1B4_5087 - Glycosyl transferase, group 1 family protein, at 5,637,315 to 5,638,394 _5087 Pf1N1B4_5088 - Glycosyl transferase, group 1 family protein, at 5,638,391 to 5,639,503 _5088 Position (kb) 5637 5638 5639Strain fitness (log2 ratio) -2 -1 0 1at 5636.355 kb on - strand, within Pf1N1B4_5085at 5636.471 kb on + strandat 5636.549 kb on + strandat 5636.549 kb on + strandat 5636.550 kb on - strandat 5636.612 kb on - strandat 5637.127 kb on + strand, within Pf1N1B4_5086at 5637.420 kb on + strandat 5637.608 kb on + strand, within Pf1N1B4_5087at 5637.627 kb on + strand, within Pf1N1B4_5087at 5637.627 kb on + strand, within Pf1N1B4_5087at 5637.627 kb on + strand, within Pf1N1B4_5087at 5637.627 kb on + strand, within Pf1N1B4_5087at 5637.628 kb on - strand, within Pf1N1B4_5087at 5637.628 kb on - strand, within Pf1N1B4_5087at 5637.702 kb on + strand, within Pf1N1B4_5087at 5637.702 kb on + strand, within Pf1N1B4_5087at 5637.703 kb on - strand, within Pf1N1B4_5087at 5637.703 kb on - strand, within Pf1N1B4_5087at 5637.899 kb on - strand, within Pf1N1B4_5087at 5637.934 kb on - strand, within Pf1N1B4_5087at 5638.345 kb on - strandat 5638.345 kb on - strandat 5638.372 kb on - strandat 5638.372 kb on - strandat 5638.374 kb on + strandat 5638.995 kb on + strand, within Pf1N1B4_5088

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
remove
5,636,355 - Pf1N1B4_5085 0.86 -2.5
5,636,471 + +0.3
5,636,549 + -2.3
5,636,549 + +0.3
5,636,550 - -0.7
5,636,612 - -0.3
5,637,127 + Pf1N1B4_5086 0.77 -0.1
5,637,420 + -0.1
5,637,608 + Pf1N1B4_5087 0.27 -0.4
5,637,627 + Pf1N1B4_5087 0.29 -1.3
5,637,627 + Pf1N1B4_5087 0.29 +1.5
5,637,627 + Pf1N1B4_5087 0.29 +1.6
5,637,627 + Pf1N1B4_5087 0.29 +0.3
5,637,628 - Pf1N1B4_5087 0.29 -0.5
5,637,628 - Pf1N1B4_5087 0.29 -0.1
5,637,702 + Pf1N1B4_5087 0.36 +0.3
5,637,702 + Pf1N1B4_5087 0.36 -0.8
5,637,703 - Pf1N1B4_5087 0.36 -2.0
5,637,703 - Pf1N1B4_5087 0.36 +0.9
5,637,899 - Pf1N1B4_5087 0.54 +0.9
5,637,934 - Pf1N1B4_5087 0.57 -0.3
5,638,345 - -0.7
5,638,345 - -1.1
5,638,372 - -1.5
5,638,372 - +0.5
5,638,374 + +0.3
5,638,995 + Pf1N1B4_5088 0.54 -1.3

Or see this region's nucleotide sequence