Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5075

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5074 and Pf1N1B4_5075 are separated by 232 nucleotidesPf1N1B4_5075 and Pf1N1B4_5076 overlap by 4 nucleotides Pf1N1B4_5074 - Gluconate permease, at 5,619,485 to 5,620,897 _5074 Pf1N1B4_5075 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 5,621,130 to 5,621,768 _5075 Pf1N1B4_5076 - FIG00641944: hypothetical protein, at 5,621,765 to 5,623,057 _5076 Position (kb) 5621 5622Strain fitness (log2 ratio) -2 -1 0 1at 5620.299 kb on + strand, within Pf1N1B4_5074at 5620.300 kb on - strandat 5620.374 kb on + strand, within Pf1N1B4_5074at 5620.375 kb on - strand, within Pf1N1B4_5074at 5620.375 kb on - strand, within Pf1N1B4_5074at 5620.534 kb on + strand, within Pf1N1B4_5074at 5620.875 kb on + strandat 5621.108 kb on - strandat 5621.524 kb on + strand, within Pf1N1B4_5075

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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5,620,299 + Pf1N1B4_5074 0.58 -1.7
5,620,300 - -0.1
5,620,374 + Pf1N1B4_5074 0.63 -2.5
5,620,375 - Pf1N1B4_5074 0.63 -1.3
5,620,375 - Pf1N1B4_5074 0.63 +0.1
5,620,534 + Pf1N1B4_5074 0.74 -0.5
5,620,875 + -0.0
5,621,108 - +0.3
5,621,524 + Pf1N1B4_5075 0.62 +0.2

Or see this region's nucleotide sequence