Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4951 and Pf1N1B4_4952 are separated by 566 nucleotides Pf1N1B4_4952 and Pf1N1B4_4953 overlap by 4 nucleotides
Pf1N1B4_4951 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 5,487,711 to 5,488,073
_4951
Pf1N1B4_4952 - L-seryl-tRNA(Sec) selenium transferase (EC 2.9.1.1), at 5,488,640 to 5,490,070
_4952
Pf1N1B4_4953 - Selenocysteine-specific translation elongation factor, at 5,490,067 to 5,491,986
_4953
Position (kb)
5488
5489
5490
5491 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5487.712 kb on + strand at 5487.713 kb on - strand at 5487.713 kb on - strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.717 kb on - strand at 5487.717 kb on - strand at 5487.717 kb on - strand at 5487.786 kb on + strand, within Pf1N1B4_4951 at 5487.787 kb on - strand at 5487.794 kb on + strand at 5487.813 kb on + strand, within Pf1N1B4_4951 at 5487.814 kb on - strand, within Pf1N1B4_4951 at 5487.814 kb on - strand, within Pf1N1B4_4951 at 5487.968 kb on - strand, within Pf1N1B4_4951 at 5487.970 kb on + strand, within Pf1N1B4_4951 at 5487.970 kb on + strand, within Pf1N1B4_4951 at 5488.073 kb on + strand at 5488.073 kb on + strand at 5488.074 kb on - strand at 5488.074 kb on - strand at 5488.076 kb on + strand at 5488.226 kb on - strand at 5488.363 kb on + strand at 5488.395 kb on + strand at 5488.395 kb on + strand at 5488.395 kb on + strand at 5488.396 kb on - strand at 5488.396 kb on - strand at 5488.412 kb on - strand at 5488.585 kb on + strand at 5488.834 kb on - strand, within Pf1N1B4_4952 at 5489.560 kb on - strand, within Pf1N1B4_4952 at 5489.779 kb on - strand, within Pf1N1B4_4952 at 5489.779 kb on - strand, within Pf1N1B4_4952 at 5490.191 kb on - strand at 5490.192 kb on + strand at 5490.192 kb on + strand at 5490.193 kb on - strand at 5490.196 kb on + strand at 5490.393 kb on + strand, within Pf1N1B4_4953
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 5,487,712 + -1.3 5,487,713 - -0.3 5,487,713 - -0.3 5,487,716 + -0.4 5,487,716 + +1.3 5,487,716 + +0.5 5,487,716 + -0.7 5,487,716 + +0.3 5,487,716 + -0.8 5,487,717 - -0.6 5,487,717 - -0.3 5,487,717 - -0.5 5,487,786 + Pf1N1B4_4951 0.21 -1.3 5,487,787 - +0.7 5,487,794 + -1.5 5,487,813 + Pf1N1B4_4951 0.28 +1.0 5,487,814 - Pf1N1B4_4951 0.28 -0.1 5,487,814 - Pf1N1B4_4951 0.28 +1.1 5,487,968 - Pf1N1B4_4951 0.71 -0.3 5,487,970 + Pf1N1B4_4951 0.71 -1.1 5,487,970 + Pf1N1B4_4951 0.71 -0.2 5,488,073 + +0.1 5,488,073 + +1.9 5,488,074 - -0.8 5,488,074 - -1.3 5,488,076 + -0.3 5,488,226 - -0.4 5,488,363 + +0.3 5,488,395 + +0.3 5,488,395 + -1.7 5,488,395 + +0.1 5,488,396 - -0.9 5,488,396 - -1.3 5,488,412 - -0.7 5,488,585 + +0.3 5,488,834 - Pf1N1B4_4952 0.14 -0.9 5,489,560 - Pf1N1B4_4952 0.64 -1.0 5,489,779 - Pf1N1B4_4952 0.80 -2.0 5,489,779 - Pf1N1B4_4952 0.80 -0.3 5,490,191 - +0.5 5,490,192 + +0.9 5,490,192 + -0.3 5,490,193 - +0.9 5,490,196 + +0.9 5,490,393 + Pf1N1B4_4953 0.17 -0.1
Or see this region's nucleotide sequence