Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4905 and Pf1N1B4_4906 are separated by 23 nucleotides Pf1N1B4_4906 and Pf1N1B4_4907 are separated by 97 nucleotides
Pf1N1B4_4905 - Serine protease homologue, at 5,431,280 to 5,434,375
_4905
Pf1N1B4_4906 - Lipase (EC 3.1.1.3), at 5,434,399 to 5,436,099
_4906
Pf1N1B4_4907 - Lipase (EC 3.1.1.3), at 5,436,197 to 5,438,050
_4907
Position (kb)
5434
5435
5436
5437 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5433.460 kb on + strand, within Pf1N1B4_4905 at 5433.698 kb on - strand, within Pf1N1B4_4905 at 5433.755 kb on + strand at 5433.755 kb on + strand, within Pf1N1B4_4905 at 5433.770 kb on + strand, within Pf1N1B4_4905 at 5433.771 kb on - strand, within Pf1N1B4_4905 at 5433.818 kb on + strand, within Pf1N1B4_4905 at 5433.859 kb on + strand, within Pf1N1B4_4905 at 5433.980 kb on + strand, within Pf1N1B4_4905 at 5433.980 kb on + strand, within Pf1N1B4_4905 at 5434.307 kb on + strand at 5434.307 kb on + strand at 5434.478 kb on - strand at 5434.582 kb on + strand, within Pf1N1B4_4906 at 5434.582 kb on + strand, within Pf1N1B4_4906 at 5434.583 kb on - strand, within Pf1N1B4_4906 at 5434.583 kb on - strand, within Pf1N1B4_4906 at 5434.633 kb on + strand, within Pf1N1B4_4906 at 5434.757 kb on - strand, within Pf1N1B4_4906 at 5434.911 kb on + strand, within Pf1N1B4_4906 at 5434.942 kb on + strand, within Pf1N1B4_4906 at 5434.943 kb on - strand at 5435.123 kb on - strand at 5435.196 kb on - strand, within Pf1N1B4_4906 at 5435.201 kb on - strand, within Pf1N1B4_4906 at 5435.239 kb on + strand at 5435.459 kb on - strand, within Pf1N1B4_4906 at 5435.554 kb on + strand, within Pf1N1B4_4906 at 5435.569 kb on + strand, within Pf1N1B4_4906 at 5435.653 kb on + strand at 5435.702 kb on - strand, within Pf1N1B4_4906 at 5435.980 kb on + strand at 5435.980 kb on + strand at 5435.980 kb on + strand at 5435.981 kb on - strand at 5435.981 kb on - strand at 5436.057 kb on - strand at 5436.057 kb on - strand at 5436.207 kb on - strand at 5436.207 kb on - strand at 5436.275 kb on + strand at 5436.275 kb on + strand at 5436.575 kb on + strand at 5436.720 kb on - strand, within Pf1N1B4_4907 at 5436.724 kb on - strand, within Pf1N1B4_4907 at 5436.893 kb on + strand, within Pf1N1B4_4907 at 5436.894 kb on - strand, within Pf1N1B4_4907 at 5437.070 kb on + strand, within Pf1N1B4_4907
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 5,433,460 + Pf1N1B4_4905 0.70 +0.5 5,433,698 - Pf1N1B4_4905 0.78 -1.9 5,433,755 + +1.3 5,433,755 + Pf1N1B4_4905 0.80 -1.3 5,433,770 + Pf1N1B4_4905 0.80 -0.4 5,433,771 - Pf1N1B4_4905 0.80 +0.9 5,433,818 + Pf1N1B4_4905 0.82 -0.5 5,433,859 + Pf1N1B4_4905 0.83 -0.4 5,433,980 + Pf1N1B4_4905 0.87 -1.4 5,433,980 + Pf1N1B4_4905 0.87 -0.1 5,434,307 + +0.7 5,434,307 + +1.6 5,434,478 - -0.4 5,434,582 + Pf1N1B4_4906 0.11 +1.3 5,434,582 + Pf1N1B4_4906 0.11 -1.7 5,434,583 - Pf1N1B4_4906 0.11 -2.9 5,434,583 - Pf1N1B4_4906 0.11 -2.0 5,434,633 + Pf1N1B4_4906 0.14 -1.8 5,434,757 - Pf1N1B4_4906 0.21 -1.4 5,434,911 + Pf1N1B4_4906 0.30 -1.0 5,434,942 + Pf1N1B4_4906 0.32 -1.3 5,434,943 - -0.3 5,435,123 - +0.3 5,435,196 - Pf1N1B4_4906 0.47 -0.9 5,435,201 - Pf1N1B4_4906 0.47 -1.6 5,435,239 + -0.7 5,435,459 - Pf1N1B4_4906 0.62 -0.7 5,435,554 + Pf1N1B4_4906 0.68 -1.3 5,435,569 + Pf1N1B4_4906 0.69 +0.3 5,435,653 + -0.7 5,435,702 - Pf1N1B4_4906 0.77 -2.3 5,435,980 + -0.5 5,435,980 + -1.1 5,435,980 + -2.3 5,435,981 - -0.7 5,435,981 - -1.0 5,436,057 - -2.0 5,436,057 - +0.8 5,436,207 - -0.4 5,436,207 - +0.3 5,436,275 + +0.3 5,436,275 + -0.2 5,436,575 + +0.9 5,436,720 - Pf1N1B4_4907 0.28 +0.3 5,436,724 - Pf1N1B4_4907 0.28 -0.1 5,436,893 + Pf1N1B4_4907 0.38 -0.0 5,436,894 - Pf1N1B4_4907 0.38 -0.5 5,437,070 + Pf1N1B4_4907 0.47 -0.3
Or see this region's nucleotide sequence