Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4892 and Pf1N1B4_4893 are separated by 51 nucleotides Pf1N1B4_4893 and Pf1N1B4_4894 are separated by 31 nucleotides Pf1N1B4_4894 and Pf1N1B4_4895 are separated by 44 nucleotides
Pf1N1B4_4892 - Transcriptional regulator, AraC family, at 5,414,306 to 5,415,121
_4892
Pf1N1B4_4893 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 5,415,173 to 5,416,579
_4893
Pf1N1B4_4894 - Nucleoside-diphosphate-sugar epimerases, at 5,416,611 to 5,416,952
_4894
Pf1N1B4_4895 - Putrescine ABC transporter, periplasmic putrescine-binding protein, at 5,416,997 to 5,418,082
_4895
Position (kb) 5415
5416
5417 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5414.306 kb on - strand at 5414.432 kb on + strand at 5414.432 kb on + strand, within Pf1N1B4_4892 at 5414.483 kb on + strand, within Pf1N1B4_4892 at 5414.483 kb on + strand, within Pf1N1B4_4892 at 5414.540 kb on - strand, within Pf1N1B4_4892 at 5414.810 kb on + strand, within Pf1N1B4_4892 at 5414.880 kb on - strand, within Pf1N1B4_4892 at 5414.913 kb on - strand, within Pf1N1B4_4892 at 5414.919 kb on - strand, within Pf1N1B4_4892 at 5415.033 kb on - strand, within Pf1N1B4_4892 at 5415.308 kb on + strand at 5415.308 kb on + strand at 5415.311 kb on + strand at 5415.311 kb on + strand at 5415.311 kb on + strand at 5415.710 kb on - strand, within Pf1N1B4_4893 at 5415.713 kb on + strand at 5415.713 kb on + strand, within Pf1N1B4_4893 at 5415.713 kb on + strand, within Pf1N1B4_4893 at 5415.713 kb on + strand, within Pf1N1B4_4893 at 5415.714 kb on - strand, within Pf1N1B4_4893 at 5415.714 kb on - strand, within Pf1N1B4_4893 at 5415.797 kb on + strand at 5416.040 kb on + strand, within Pf1N1B4_4893 at 5416.041 kb on - strand at 5416.041 kb on - strand, within Pf1N1B4_4893 at 5416.041 kb on - strand, within Pf1N1B4_4893 at 5416.041 kb on - strand at 5416.071 kb on + strand, within Pf1N1B4_4893 at 5416.143 kb on + strand, within Pf1N1B4_4893 at 5416.144 kb on - strand, within Pf1N1B4_4893 at 5416.228 kb on + strand, within Pf1N1B4_4893 at 5416.228 kb on + strand, within Pf1N1B4_4893 at 5416.604 kb on + strand at 5416.605 kb on - strand at 5416.854 kb on + strand, within Pf1N1B4_4894 at 5416.854 kb on + strand at 5416.855 kb on - strand at 5416.855 kb on - strand at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.936 kb on - strand at 5416.936 kb on - strand at 5417.148 kb on - strand, within Pf1N1B4_4895 at 5417.369 kb on + strand, within Pf1N1B4_4895 at 5417.369 kb on + strand, within Pf1N1B4_4895 at 5417.550 kb on - strand, within Pf1N1B4_4895 at 5417.550 kb on - strand, within Pf1N1B4_4895 at 5417.550 kb on - strand, within Pf1N1B4_4895
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 5,414,306 - +0.5 5,414,432 + -0.7 5,414,432 + Pf1N1B4_4892 0.15 +0.7 5,414,483 + Pf1N1B4_4892 0.22 +1.3 5,414,483 + Pf1N1B4_4892 0.22 +0.3 5,414,540 - Pf1N1B4_4892 0.29 -0.7 5,414,810 + Pf1N1B4_4892 0.62 -0.9 5,414,880 - Pf1N1B4_4892 0.70 +0.9 5,414,913 - Pf1N1B4_4892 0.74 +0.3 5,414,919 - Pf1N1B4_4892 0.75 -2.5 5,415,033 - Pf1N1B4_4892 0.89 +1.5 5,415,308 + +0.6 5,415,308 + -0.9 5,415,311 + -0.7 5,415,311 + -1.6 5,415,311 + +2.9 5,415,710 - Pf1N1B4_4893 0.38 +0.9 5,415,713 + +0.3 5,415,713 + Pf1N1B4_4893 0.38 +0.3 5,415,713 + Pf1N1B4_4893 0.38 +0.3 5,415,713 + Pf1N1B4_4893 0.38 -0.5 5,415,714 - Pf1N1B4_4893 0.38 +0.3 5,415,714 - Pf1N1B4_4893 0.38 +1.3 5,415,797 + +0.3 5,416,040 + Pf1N1B4_4893 0.62 +0.9 5,416,041 - -0.7 5,416,041 - Pf1N1B4_4893 0.62 +1.9 5,416,041 - Pf1N1B4_4893 0.62 +0.3 5,416,041 - -0.7 5,416,071 + Pf1N1B4_4893 0.64 +0.6 5,416,143 + Pf1N1B4_4893 0.69 +0.3 5,416,144 - Pf1N1B4_4893 0.69 -0.2 5,416,228 + Pf1N1B4_4893 0.75 -0.2 5,416,228 + Pf1N1B4_4893 0.75 -0.7 5,416,604 + -0.3 5,416,605 - -1.3 5,416,854 + Pf1N1B4_4894 0.71 -0.7 5,416,854 + +0.3 5,416,855 - -0.7 5,416,855 - -0.1 5,416,859 - Pf1N1B4_4894 0.73 -2.7 5,416,859 - Pf1N1B4_4894 0.73 +0.3 5,416,859 - Pf1N1B4_4894 0.73 -1.0 5,416,859 - Pf1N1B4_4894 0.73 +1.3 5,416,859 - Pf1N1B4_4894 0.73 +2.9 5,416,859 - Pf1N1B4_4894 0.73 +0.1 5,416,859 - Pf1N1B4_4894 0.73 +1.1 5,416,859 - Pf1N1B4_4894 0.73 +2.0 5,416,859 - Pf1N1B4_4894 0.73 +0.3 5,416,936 - +0.8 5,416,936 - -0.7 5,417,148 - Pf1N1B4_4895 0.14 -0.7 5,417,369 + Pf1N1B4_4895 0.34 +0.3 5,417,369 + Pf1N1B4_4895 0.34 -1.5 5,417,550 - Pf1N1B4_4895 0.51 -0.7 5,417,550 - Pf1N1B4_4895 0.51 -1.4 5,417,550 - Pf1N1B4_4895 0.51 -0.1
Or see this region's nucleotide sequence