Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4830 and Pf1N1B4_4831 are separated by 5 nucleotides Pf1N1B4_4831 and Pf1N1B4_4832 are separated by 562 nucleotides Pf1N1B4_4832 and Pf1N1B4_4833 are separated by 7 nucleotides
Pf1N1B4_4830 - Membrane fusion component of tripartite multidrug resistance system, at 5,334,281 to 5,335,420
_4830
Pf1N1B4_4831 - Outer membrane protein assembly factor YaeT precursor, at 5,335,426 to 5,336,895
_4831
Pf1N1B4_4832 - hypothetical protein, at 5,337,458 to 5,337,664
_4832
Pf1N1B4_4833 - DNA-binding response regulator, LuxR family, at 5,337,672 to 5,338,304
_4833
Position (kb) 5335
5336
5337 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5334.765 kb on - strand, within Pf1N1B4_4830 at 5335.268 kb on - strand at 5335.270 kb on - strand, within Pf1N1B4_4830 at 5335.297 kb on - strand, within Pf1N1B4_4830 at 5335.616 kb on + strand, within Pf1N1B4_4831 at 5335.616 kb on + strand, within Pf1N1B4_4831 at 5335.617 kb on - strand, within Pf1N1B4_4831 at 5335.864 kb on - strand, within Pf1N1B4_4831 at 5336.218 kb on + strand, within Pf1N1B4_4831 at 5336.219 kb on - strand, within Pf1N1B4_4831 at 5336.963 kb on + strand at 5337.036 kb on + strand at 5337.172 kb on + strand at 5337.382 kb on + strand at 5337.497 kb on + strand, within Pf1N1B4_4832 at 5337.497 kb on + strand, within Pf1N1B4_4832 at 5337.498 kb on - strand, within Pf1N1B4_4832 at 5337.498 kb on - strand, within Pf1N1B4_4832 at 5337.501 kb on + strand, within Pf1N1B4_4832 at 5337.501 kb on + strand, within Pf1N1B4_4832 at 5337.501 kb on + strand, within Pf1N1B4_4832 at 5337.501 kb on + strand, within Pf1N1B4_4832 at 5337.502 kb on - strand, within Pf1N1B4_4832 at 5337.502 kb on - strand, within Pf1N1B4_4832 at 5337.502 kb on - strand, within Pf1N1B4_4832 at 5337.505 kb on + strand, within Pf1N1B4_4832 at 5337.508 kb on + strand, within Pf1N1B4_4832 at 5337.508 kb on + strand, within Pf1N1B4_4832 at 5337.532 kb on + strand at 5337.625 kb on + strand, within Pf1N1B4_4832 at 5337.768 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 5,334,765 - Pf1N1B4_4830 0.42 -1.0 5,335,268 - +0.3 5,335,270 - Pf1N1B4_4830 0.87 +0.4 5,335,297 - Pf1N1B4_4830 0.89 -0.3 5,335,616 + Pf1N1B4_4831 0.13 +0.7 5,335,616 + Pf1N1B4_4831 0.13 -1.1 5,335,617 - Pf1N1B4_4831 0.13 +0.3 5,335,864 - Pf1N1B4_4831 0.30 -0.0 5,336,218 + Pf1N1B4_4831 0.54 +0.9 5,336,219 - Pf1N1B4_4831 0.54 +1.1 5,336,963 + +1.5 5,337,036 + +0.0 5,337,172 + -1.5 5,337,382 + +1.3 5,337,497 + Pf1N1B4_4832 0.19 +1.6 5,337,497 + Pf1N1B4_4832 0.19 -2.5 5,337,498 - Pf1N1B4_4832 0.19 +0.8 5,337,498 - Pf1N1B4_4832 0.19 -0.2 5,337,501 + Pf1N1B4_4832 0.21 +0.7 5,337,501 + Pf1N1B4_4832 0.21 -0.2 5,337,501 + Pf1N1B4_4832 0.21 -0.7 5,337,501 + Pf1N1B4_4832 0.21 -1.7 5,337,502 - Pf1N1B4_4832 0.21 +1.3 5,337,502 - Pf1N1B4_4832 0.21 -0.9 5,337,502 - Pf1N1B4_4832 0.21 +0.1 5,337,505 + Pf1N1B4_4832 0.23 +0.3 5,337,508 + Pf1N1B4_4832 0.24 -0.1 5,337,508 + Pf1N1B4_4832 0.24 +0.3 5,337,532 + +0.3 5,337,625 + Pf1N1B4_4832 0.81 +0.3 5,337,768 + +2.1
Or see this region's nucleotide sequence