Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4809 and Pf1N1B4_4810 overlap by 4 nucleotides Pf1N1B4_4810 and Pf1N1B4_4811 overlap by 4 nucleotides Pf1N1B4_4811 and Pf1N1B4_4812 are separated by 575 nucleotides
Pf1N1B4_4809 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 5,312,015 to 5,313,394
_4809
Pf1N1B4_4810 - Polymyxin resistance protein PmrM, at 5,313,391 to 5,313,804
_4810
Pf1N1B4_4811 - Polymyxin resistance protein PmrL, sucrose-6 phosphate hydrolase, at 5,313,801 to 5,314,145
_4811
Pf1N1B4_4812 - Site-specific recombinase, phage integrase family, at 5,314,721 to 5,315,692
_4812
Position (kb)
5313
5314 Strain fitness (log2 ratio)
-1
0
1 at 5312.466 kb on + strand, within Pf1N1B4_4809 at 5312.466 kb on + strand, within Pf1N1B4_4809 at 5313.367 kb on - strand at 5313.381 kb on - strand at 5313.530 kb on + strand at 5313.530 kb on + strand, within Pf1N1B4_4810 at 5313.543 kb on - strand, within Pf1N1B4_4810 at 5313.563 kb on + strand, within Pf1N1B4_4810 at 5313.663 kb on + strand, within Pf1N1B4_4810 at 5314.129 kb on - strand at 5314.356 kb on - strand at 5314.379 kb on + strand at 5314.379 kb on + strand at 5314.380 kb on - strand at 5314.380 kb on - strand at 5314.663 kb on - strand at 5314.691 kb on - strand at 5314.693 kb on - strand at 5314.700 kb on - strand at 5314.765 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 5,312,466 + Pf1N1B4_4809 0.33 +1.3 5,312,466 + Pf1N1B4_4809 0.33 +0.4 5,313,367 - +0.3 5,313,381 - +0.4 5,313,530 + +0.3 5,313,530 + Pf1N1B4_4810 0.34 +0.3 5,313,543 - Pf1N1B4_4810 0.37 +0.5 5,313,563 + Pf1N1B4_4810 0.42 -0.4 5,313,663 + Pf1N1B4_4810 0.66 +0.6 5,314,129 - -0.3 5,314,356 - +1.3 5,314,379 + -1.0 5,314,379 + +0.1 5,314,380 - +0.9 5,314,380 - +0.9 5,314,663 - +0.3 5,314,691 - -0.9 5,314,693 - -1.0 5,314,700 - -0.5 5,314,765 - +0.3
Or see this region's nucleotide sequence