Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4552 and Pf1N1B4_4553 are separated by 46 nucleotides Pf1N1B4_4553 and Pf1N1B4_4554 are separated by 9 nucleotides Pf1N1B4_4554 and Pf1N1B4_4555 are separated by 127 nucleotides Pf1N1B4_4555 and Pf1N1B4_4556 are separated by 53 nucleotides
Pf1N1B4_4552 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41), at 5,020,720 to 5,021,940
_4552
Pf1N1B4_4553 - FIG00959354: hypothetical protein, at 5,021,987 to 5,024,857
_4553
Pf1N1B4_4554 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 5,024,867 to 5,025,355
_4554
Pf1N1B4_4555 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 5,025,483 to 5,025,602
_4555
Pf1N1B4_4556 - D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 5,025,656 to 5,026,363
_4556
Position (kb) 5021
5022
5023
5024
5025 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5021.205 kb on + strand, within Pf1N1B4_4552 at 5021.205 kb on + strand, within Pf1N1B4_4552 at 5021.205 kb on + strand, within Pf1N1B4_4552 at 5021.260 kb on + strand, within Pf1N1B4_4552 at 5021.260 kb on + strand at 5021.260 kb on + strand, within Pf1N1B4_4552 at 5021.260 kb on + strand, within Pf1N1B4_4552 at 5021.260 kb on + strand, within Pf1N1B4_4552 at 5021.261 kb on - strand, within Pf1N1B4_4552 at 5021.340 kb on + strand, within Pf1N1B4_4552 at 5021.341 kb on - strand, within Pf1N1B4_4552 at 5021.600 kb on + strand, within Pf1N1B4_4552 at 5021.679 kb on + strand, within Pf1N1B4_4552 at 5021.679 kb on + strand, within Pf1N1B4_4552 at 5021.680 kb on - strand at 5021.680 kb on - strand, within Pf1N1B4_4552 at 5021.680 kb on - strand, within Pf1N1B4_4552 at 5021.999 kb on - strand at 5022.077 kb on - strand at 5022.086 kb on + strand at 5022.786 kb on + strand, within Pf1N1B4_4553 at 5022.881 kb on + strand at 5023.370 kb on + strand, within Pf1N1B4_4553 at 5023.558 kb on + strand, within Pf1N1B4_4553 at 5023.559 kb on - strand, within Pf1N1B4_4553 at 5023.881 kb on - strand, within Pf1N1B4_4553 at 5023.980 kb on - strand at 5023.980 kb on - strand, within Pf1N1B4_4553 at 5024.391 kb on + strand, within Pf1N1B4_4553 at 5024.392 kb on - strand, within Pf1N1B4_4553 at 5024.392 kb on - strand, within Pf1N1B4_4553 at 5024.392 kb on - strand, within Pf1N1B4_4553 at 5024.488 kb on - strand at 5024.488 kb on - strand, within Pf1N1B4_4553 at 5024.615 kb on + strand at 5024.699 kb on + strand at 5024.855 kb on + strand at 5024.966 kb on + strand, within Pf1N1B4_4554 at 5024.967 kb on - strand, within Pf1N1B4_4554 at 5025.269 kb on - strand, within Pf1N1B4_4554 at 5025.324 kb on - strand at 5025.531 kb on + strand, within Pf1N1B4_4555 at 5025.532 kb on - strand, within Pf1N1B4_4555 at 5025.532 kb on - strand, within Pf1N1B4_4555 at 5025.532 kb on - strand, within Pf1N1B4_4555 at 5025.532 kb on - strand, within Pf1N1B4_4555 at 5025.644 kb on - strand at 5025.770 kb on + strand at 5025.772 kb on + strand at 5025.839 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 5,021,205 + Pf1N1B4_4552 0.40 -1.2 5,021,205 + Pf1N1B4_4552 0.40 -1.2 5,021,205 + Pf1N1B4_4552 0.40 -2.4 5,021,260 + Pf1N1B4_4552 0.44 -1.3 5,021,260 + -0.6 5,021,260 + Pf1N1B4_4552 0.44 +1.2 5,021,260 + Pf1N1B4_4552 0.44 +0.7 5,021,260 + Pf1N1B4_4552 0.44 -0.1 5,021,261 - Pf1N1B4_4552 0.44 +0.1 5,021,340 + Pf1N1B4_4552 0.51 -0.3 5,021,341 - Pf1N1B4_4552 0.51 +1.2 5,021,600 + Pf1N1B4_4552 0.72 +1.6 5,021,679 + Pf1N1B4_4552 0.79 +1.4 5,021,679 + Pf1N1B4_4552 0.79 +0.4 5,021,680 - -0.6 5,021,680 - Pf1N1B4_4552 0.79 +1.6 5,021,680 - Pf1N1B4_4552 0.79 -1.9 5,021,999 - -0.6 5,022,077 - +0.6 5,022,086 + -0.7 5,022,786 + Pf1N1B4_4553 0.28 +1.0 5,022,881 + +1.4 5,023,370 + Pf1N1B4_4553 0.48 +1.6 5,023,558 + Pf1N1B4_4553 0.55 +1.4 5,023,559 - Pf1N1B4_4553 0.55 -0.4 5,023,881 - Pf1N1B4_4553 0.66 +1.4 5,023,980 - +0.4 5,023,980 - Pf1N1B4_4553 0.69 +1.6 5,024,391 + Pf1N1B4_4553 0.84 -1.8 5,024,392 - Pf1N1B4_4553 0.84 +0.2 5,024,392 - Pf1N1B4_4553 0.84 -0.9 5,024,392 - Pf1N1B4_4553 0.84 +1.4 5,024,488 - +1.0 5,024,488 - Pf1N1B4_4553 0.87 +0.7 5,024,615 + -2.8 5,024,699 + +0.1 5,024,855 + -1.2 5,024,966 + Pf1N1B4_4554 0.20 -0.8 5,024,967 - Pf1N1B4_4554 0.20 +1.8 5,025,269 - Pf1N1B4_4554 0.82 -0.6 5,025,324 - -1.2 5,025,531 + Pf1N1B4_4555 0.40 -0.2 5,025,532 - Pf1N1B4_4555 0.41 -1.0 5,025,532 - Pf1N1B4_4555 0.41 -1.2 5,025,532 - Pf1N1B4_4555 0.41 -0.3 5,025,532 - Pf1N1B4_4555 0.41 -0.2 5,025,644 - -0.4 5,025,770 + -0.9 5,025,772 + +2.0 5,025,839 + +0.7
Or see this region's nucleotide sequence