Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4518

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	1-ethyl-3-methylimidazolium lysine 5 mM
4,980,716	+		Pf1N1B4_4517	0.75	-0.6
4,980,891	+		Pf1N1B4_4517	0.87	-0.7
4,980,891	+		Pf1N1B4_4517	0.87	-1.0
4,980,891	+		Pf1N1B4_4517	0.87	-1.0
4,980,891	+		Pf1N1B4_4517	0.87	+0.3
4,980,892	-				+1.4
4,981,010	+				+0.4
4,981,025	+				+0.1
4,981,042	-				-1.1
4,981,115	+				-0.3
4,981,125	-				+0.0
4,981,149	+				-0.1
4,981,782	-				-0.9
4,981,880	+		Pf1N1B4_4518	0.12	+0.3
4,981,881	-		Pf1N1B4_4518	0.12	-2.6
4,982,065	-		Pf1N1B4_4518	0.25	-3.4
4,982,400	-		Pf1N1B4_4518	0.47	-0.6
4,982,748	-		Pf1N1B4_4518	0.71	-0.6
4,982,748	-				+0.0
4,982,847	+		Pf1N1B4_4518	0.77	-1.0
4,982,865	-		Pf1N1B4_4518	0.79	+0.4
4,983,284	-				-1.0
4,983,284	-				-0.8
4,984,146	-		Pf1N1B4_4519	0.65	+0.4