Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4194 and Pf1N1B4_4195 are separated by 146 nucleotides Pf1N1B4_4195 and Pf1N1B4_4196 overlap by 37 nucleotides Pf1N1B4_4196 and Pf1N1B4_4197 overlap by 2 nucleotides Pf1N1B4_4197 and Pf1N1B4_4198 are separated by 282 nucleotides
Pf1N1B4_4194 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 4,646,665 to 4,647,786
_4194
Pf1N1B4_4195 - Transcriptional regulator, TetR family, at 4,647,933 to 4,648,505
_4195
Pf1N1B4_4196 - hypothetical protein, at 4,648,469 to 4,648,630
_4196
Pf1N1B4_4197 - Glucose ABC transport system, periplasmic sugar-binding protein, at 4,648,629 to 4,648,754
_4197
Pf1N1B4_4198 - 4-hydroxybenzoate transporter, at 4,649,037 to 4,650,374
_4198
Position (kb)
4647
4648
4649 Strain fitness (log2 ratio)
-2
-1
0
1 at 4647.246 kb on - strand, within Pf1N1B4_4194 at 4647.563 kb on + strand at 4647.657 kb on - strand, within Pf1N1B4_4194 at 4647.766 kb on - strand at 4647.821 kb on - strand at 4647.886 kb on + strand at 4647.887 kb on - strand at 4647.890 kb on + strand at 4648.185 kb on + strand at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.329 kb on + strand, within Pf1N1B4_4195 at 4648.800 kb on + strand at 4648.874 kb on - strand at 4648.952 kb on + strand at 4648.952 kb on + strand at 4649.015 kb on - strand at 4649.022 kb on + strand at 4649.022 kb on + strand at 4649.117 kb on - strand at 4649.128 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 4,647,246 - Pf1N1B4_4194 0.52 +1.3 4,647,563 + +0.5 4,647,657 - Pf1N1B4_4194 0.88 -1.1 4,647,766 - -0.4 4,647,821 - +0.3 4,647,886 + +0.0 4,647,887 - +0.8 4,647,890 + -1.3 4,648,185 + +0.5 4,648,185 + Pf1N1B4_4195 0.44 +0.9 4,648,185 + Pf1N1B4_4195 0.44 -0.5 4,648,185 + Pf1N1B4_4195 0.44 +1.1 4,648,185 + Pf1N1B4_4195 0.44 +0.3 4,648,185 + Pf1N1B4_4195 0.44 +0.5 4,648,185 + Pf1N1B4_4195 0.44 +0.0 4,648,185 + -0.5 4,648,185 + Pf1N1B4_4195 0.44 +1.3 4,648,185 + -0.8 4,648,186 - Pf1N1B4_4195 0.44 +1.5 4,648,186 - Pf1N1B4_4195 0.44 -0.5 4,648,186 - Pf1N1B4_4195 0.44 -1.5 4,648,186 - +0.5 4,648,186 - Pf1N1B4_4195 0.44 +0.4 4,648,186 - Pf1N1B4_4195 0.44 +1.5 4,648,329 + Pf1N1B4_4195 0.69 -0.2 4,648,800 + -0.3 4,648,874 - -1.8 4,648,952 + -2.1 4,648,952 + -0.5 4,649,015 - -0.5 4,649,022 + +1.7 4,649,022 + -0.8 4,649,117 - -1.1 4,649,128 + -1.5
Or see this region's nucleotide sequence