Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4171 and Pf1N1B4_4172 are separated by 38 nucleotides Pf1N1B4_4172 and Pf1N1B4_4173 are separated by 55 nucleotides Pf1N1B4_4173 and Pf1N1B4_4174 are separated by 55 nucleotides Pf1N1B4_4174 and Pf1N1B4_4175 overlap by 15 nucleotides
Pf1N1B4_4171 - sensor histidine kinase, at 4,620,911 to 4,622,731
_4171
Pf1N1B4_4172 - hypothetical protein, at 4,622,770 to 4,622,904
_4172
Pf1N1B4_4173 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 4,622,960 to 4,623,523
_4173
Pf1N1B4_4174 - hypothetical protein, at 4,623,579 to 4,623,761
_4174
Pf1N1B4_4175 - Universal stress protein family 5, at 4,623,747 to 4,624,667
_4175
Position (kb) 4622
4623 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4621.902 kb on + strand at 4621.903 kb on - strand, within Pf1N1B4_4171 at 4621.903 kb on - strand, within Pf1N1B4_4171 at 4621.903 kb on - strand, within Pf1N1B4_4171 at 4621.903 kb on - strand, within Pf1N1B4_4171 at 4621.934 kb on + strand, within Pf1N1B4_4171 at 4621.935 kb on - strand at 4621.935 kb on - strand, within Pf1N1B4_4171 at 4621.954 kb on - strand, within Pf1N1B4_4171 at 4622.102 kb on + strand, within Pf1N1B4_4171 at 4622.151 kb on + strand, within Pf1N1B4_4171 at 4622.171 kb on - strand, within Pf1N1B4_4171 at 4622.208 kb on + strand, within Pf1N1B4_4171 at 4622.209 kb on - strand, within Pf1N1B4_4171 at 4622.209 kb on - strand, within Pf1N1B4_4171 at 4622.209 kb on - strand, within Pf1N1B4_4171 at 4622.209 kb on - strand, within Pf1N1B4_4171 at 4622.317 kb on + strand, within Pf1N1B4_4171 at 4622.348 kb on + strand, within Pf1N1B4_4171 at 4622.464 kb on + strand, within Pf1N1B4_4171 at 4622.465 kb on - strand, within Pf1N1B4_4171 at 4622.620 kb on - strand at 4622.641 kb on - strand at 4622.666 kb on + strand at 4622.666 kb on + strand at 4622.837 kb on + strand, within Pf1N1B4_4172 at 4622.837 kb on + strand, within Pf1N1B4_4172 at 4622.838 kb on - strand, within Pf1N1B4_4172 at 4622.838 kb on - strand, within Pf1N1B4_4172 at 4622.843 kb on + strand, within Pf1N1B4_4172 at 4622.967 kb on + strand at 4622.967 kb on + strand at 4623.075 kb on + strand, within Pf1N1B4_4173 at 4623.075 kb on + strand, within Pf1N1B4_4173 at 4623.076 kb on - strand, within Pf1N1B4_4173 at 4623.076 kb on - strand, within Pf1N1B4_4173 at 4623.261 kb on + strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.385 kb on - strand, within Pf1N1B4_4173 at 4623.667 kb on - strand, within Pf1N1B4_4174 at 4623.709 kb on + strand, within Pf1N1B4_4174 at 4623.709 kb on + strand, within Pf1N1B4_4174 at 4623.792 kb on + strand at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.869 kb on - strand, within Pf1N1B4_4175 at 4623.869 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 4,621,902 + +0.1 4,621,903 - Pf1N1B4_4171 0.54 +1.6 4,621,903 - Pf1N1B4_4171 0.54 -1.0 4,621,903 - Pf1N1B4_4171 0.54 +0.2 4,621,903 - Pf1N1B4_4171 0.54 -0.3 4,621,934 + Pf1N1B4_4171 0.56 +0.4 4,621,935 - +1.6 4,621,935 - Pf1N1B4_4171 0.56 -0.7 4,621,954 - Pf1N1B4_4171 0.57 -0.8 4,622,102 + Pf1N1B4_4171 0.65 +1.4 4,622,151 + Pf1N1B4_4171 0.68 +0.8 4,622,171 - Pf1N1B4_4171 0.69 +3.1 4,622,208 + Pf1N1B4_4171 0.71 +0.3 4,622,209 - Pf1N1B4_4171 0.71 +1.1 4,622,209 - Pf1N1B4_4171 0.71 +0.4 4,622,209 - Pf1N1B4_4171 0.71 -2.0 4,622,209 - Pf1N1B4_4171 0.71 -0.3 4,622,317 + Pf1N1B4_4171 0.77 +1.0 4,622,348 + Pf1N1B4_4171 0.79 +0.9 4,622,464 + Pf1N1B4_4171 0.85 -1.0 4,622,465 - Pf1N1B4_4171 0.85 -0.9 4,622,620 - -1.8 4,622,641 - -0.9 4,622,666 + -0.4 4,622,666 + +0.2 4,622,837 + Pf1N1B4_4172 0.50 +0.7 4,622,837 + Pf1N1B4_4172 0.50 +0.5 4,622,838 - Pf1N1B4_4172 0.50 +2.1 4,622,838 - Pf1N1B4_4172 0.50 -2.9 4,622,843 + Pf1N1B4_4172 0.54 -1.3 4,622,967 + -0.5 4,622,967 + +1.1 4,623,075 + Pf1N1B4_4173 0.20 -0.8 4,623,075 + Pf1N1B4_4173 0.20 -1.0 4,623,076 - Pf1N1B4_4173 0.21 -2.0 4,623,076 - Pf1N1B4_4173 0.21 -1.5 4,623,261 + Pf1N1B4_4173 0.53 -2.4 4,623,262 - Pf1N1B4_4173 0.54 -0.2 4,623,262 - Pf1N1B4_4173 0.54 -1.4 4,623,262 - Pf1N1B4_4173 0.54 -0.5 4,623,262 - Pf1N1B4_4173 0.54 +0.1 4,623,262 - Pf1N1B4_4173 0.54 -2.0 4,623,262 - Pf1N1B4_4173 0.54 -0.0 4,623,262 - Pf1N1B4_4173 0.54 +1.1 4,623,385 - Pf1N1B4_4173 0.75 -0.1 4,623,667 - Pf1N1B4_4174 0.48 -0.5 4,623,709 + Pf1N1B4_4174 0.71 -0.6 4,623,709 + Pf1N1B4_4174 0.71 +1.1 4,623,792 + -0.9 4,623,868 + Pf1N1B4_4175 0.13 -0.0 4,623,868 + Pf1N1B4_4175 0.13 -0.7 4,623,868 + Pf1N1B4_4175 0.13 -2.0 4,623,868 + Pf1N1B4_4175 0.13 +0.9 4,623,869 - Pf1N1B4_4175 0.13 -0.5 4,623,869 - +0.5
Or see this region's nucleotide sequence