Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4067

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4066 and Pf1N1B4_4067 are separated by 90 nucleotidesPf1N1B4_4067 and Pf1N1B4_4068 are separated by 73 nucleotidesPf1N1B4_4068 and Pf1N1B4_4069 overlap by 4 nucleotidesPf1N1B4_4069 and Pf1N1B4_4070 overlap by 1 nucleotidesPf1N1B4_4070 and Pf1N1B4_4071 are separated by 1 nucleotides Pf1N1B4_4066 - Glutathione S-transferase, omega (EC 2.5.1.18), at 4,520,824 to 4,521,828 _4066 Pf1N1B4_4067 - Anthranilate phosphoribosyltransferase like (EC 2.4.2.18), at 4,521,919 to 4,522,920 _4067 Pf1N1B4_4068 - tRNA 2-thiouridine synthesizing protein E (EC 2.8.1.-), at 4,522,994 to 4,523,251 _4068 Pf1N1B4_4069 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusB, at 4,523,248 to 4,523,541 _4069 Pf1N1B4_4070 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusC, at 4,523,541 to 4,523,903 _4070 Pf1N1B4_4071 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusD, at 4,523,905 to 4,524,327 _4071 Position (kb) 4521 4522 4523Strain fitness (log2 ratio) -2 -1 0 1 2at 4520.949 kb on - strand, within Pf1N1B4_4066at 4520.949 kb on - strand, within Pf1N1B4_4066at 4520.951 kb on + strand, within Pf1N1B4_4066at 4520.952 kb on - strand, within Pf1N1B4_4066at 4520.952 kb on - strand, within Pf1N1B4_4066at 4520.952 kb on - strand, within Pf1N1B4_4066at 4520.952 kb on - strand, within Pf1N1B4_4066at 4521.006 kb on - strand, within Pf1N1B4_4066at 4521.006 kb on - strand, within Pf1N1B4_4066at 4521.080 kb on + strand, within Pf1N1B4_4066at 4521.080 kb on + strand, within Pf1N1B4_4066at 4521.080 kb on + strand, within Pf1N1B4_4066at 4521.081 kb on - strand, within Pf1N1B4_4066at 4521.200 kb on - strandat 4521.221 kb on + strand, within Pf1N1B4_4066at 4521.222 kb on - strand, within Pf1N1B4_4066at 4521.222 kb on - strand, within Pf1N1B4_4066at 4521.479 kb on + strand, within Pf1N1B4_4066at 4521.662 kb on + strandat 4521.663 kb on - strand, within Pf1N1B4_4066at 4521.788 kb on + strandat 4521.817 kb on + strandat 4521.868 kb on + strandat 4521.911 kb on - strandat 4522.041 kb on + strandat 4522.041 kb on + strand, within Pf1N1B4_4067at 4522.041 kb on + strand, within Pf1N1B4_4067at 4522.042 kb on - strand, within Pf1N1B4_4067at 4522.042 kb on - strand, within Pf1N1B4_4067at 4522.167 kb on - strand, within Pf1N1B4_4067at 4522.167 kb on - strand, within Pf1N1B4_4067at 4522.628 kb on + strand, within Pf1N1B4_4067at 4522.629 kb on - strand, within Pf1N1B4_4067at 4522.911 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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4,520,949 - Pf1N1B4_4066 0.12 -1.2
4,520,949 - Pf1N1B4_4066 0.12 +0.6
4,520,951 + Pf1N1B4_4066 0.13 -0.1
4,520,952 - Pf1N1B4_4066 0.13 +1.4
4,520,952 - Pf1N1B4_4066 0.13 +0.2
4,520,952 - Pf1N1B4_4066 0.13 +0.2
4,520,952 - Pf1N1B4_4066 0.13 -0.7
4,521,006 - Pf1N1B4_4066 0.18 -2.0
4,521,006 - Pf1N1B4_4066 0.18 -0.8
4,521,080 + Pf1N1B4_4066 0.25 +0.3
4,521,080 + Pf1N1B4_4066 0.25 +0.4
4,521,080 + Pf1N1B4_4066 0.25 +1.4
4,521,081 - Pf1N1B4_4066 0.26 -0.4
4,521,200 - -1.0
4,521,221 + Pf1N1B4_4066 0.40 -0.3
4,521,222 - Pf1N1B4_4066 0.40 -1.3
4,521,222 - Pf1N1B4_4066 0.40 +0.9
4,521,479 + Pf1N1B4_4066 0.65 +0.6
4,521,662 + +2.2
4,521,663 - Pf1N1B4_4066 0.83 +0.8
4,521,788 + +0.6
4,521,817 + +0.0
4,521,868 + -0.0
4,521,911 - +0.6
4,522,041 + +0.6
4,522,041 + Pf1N1B4_4067 0.12 -0.2
4,522,041 + Pf1N1B4_4067 0.12 -1.4
4,522,042 - Pf1N1B4_4067 0.12 -0.4
4,522,042 - Pf1N1B4_4067 0.12 -2.2
4,522,167 - Pf1N1B4_4067 0.25 -0.6
4,522,167 - Pf1N1B4_4067 0.25 -0.4
4,522,628 + Pf1N1B4_4067 0.71 -1.5
4,522,629 - Pf1N1B4_4067 0.71 -1.1
4,522,911 - +2.2

Or see this region's nucleotide sequence