Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4066 and Pf1N1B4_4067 are separated by 90 nucleotides Pf1N1B4_4067 and Pf1N1B4_4068 are separated by 73 nucleotides Pf1N1B4_4068 and Pf1N1B4_4069 overlap by 4 nucleotides Pf1N1B4_4069 and Pf1N1B4_4070 overlap by 1 nucleotides Pf1N1B4_4070 and Pf1N1B4_4071 are separated by 1 nucleotides
Pf1N1B4_4066 - Glutathione S-transferase, omega (EC 2.5.1.18), at 4,520,824 to 4,521,828
_4066
Pf1N1B4_4067 - Anthranilate phosphoribosyltransferase like (EC 2.4.2.18), at 4,521,919 to 4,522,920
_4067
Pf1N1B4_4068 - tRNA 2-thiouridine synthesizing protein E (EC 2.8.1.-), at 4,522,994 to 4,523,251
_4068
Pf1N1B4_4069 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusB, at 4,523,248 to 4,523,541
_4069
Pf1N1B4_4070 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusC, at 4,523,541 to 4,523,903
_4070
Pf1N1B4_4071 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusD, at 4,523,905 to 4,524,327
_4071
Position (kb)
4521
4522
4523 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4520.949 kb on - strand, within Pf1N1B4_4066 at 4520.949 kb on - strand, within Pf1N1B4_4066 at 4520.951 kb on + strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4521.006 kb on - strand, within Pf1N1B4_4066 at 4521.006 kb on - strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.081 kb on - strand, within Pf1N1B4_4066 at 4521.200 kb on - strand at 4521.221 kb on + strand, within Pf1N1B4_4066 at 4521.222 kb on - strand, within Pf1N1B4_4066 at 4521.222 kb on - strand, within Pf1N1B4_4066 at 4521.479 kb on + strand, within Pf1N1B4_4066 at 4521.662 kb on + strand at 4521.663 kb on - strand, within Pf1N1B4_4066 at 4521.788 kb on + strand at 4521.817 kb on + strand at 4521.868 kb on + strand at 4521.911 kb on - strand at 4522.041 kb on + strand at 4522.041 kb on + strand, within Pf1N1B4_4067 at 4522.041 kb on + strand, within Pf1N1B4_4067 at 4522.042 kb on - strand, within Pf1N1B4_4067 at 4522.042 kb on - strand, within Pf1N1B4_4067 at 4522.167 kb on - strand, within Pf1N1B4_4067 at 4522.167 kb on - strand, within Pf1N1B4_4067 at 4522.628 kb on + strand, within Pf1N1B4_4067 at 4522.629 kb on - strand, within Pf1N1B4_4067 at 4522.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 4,520,949 - Pf1N1B4_4066 0.12 -1.2 4,520,949 - Pf1N1B4_4066 0.12 +0.6 4,520,951 + Pf1N1B4_4066 0.13 -0.1 4,520,952 - Pf1N1B4_4066 0.13 +1.4 4,520,952 - Pf1N1B4_4066 0.13 +0.2 4,520,952 - Pf1N1B4_4066 0.13 +0.2 4,520,952 - Pf1N1B4_4066 0.13 -0.7 4,521,006 - Pf1N1B4_4066 0.18 -2.0 4,521,006 - Pf1N1B4_4066 0.18 -0.8 4,521,080 + Pf1N1B4_4066 0.25 +0.3 4,521,080 + Pf1N1B4_4066 0.25 +0.4 4,521,080 + Pf1N1B4_4066 0.25 +1.4 4,521,081 - Pf1N1B4_4066 0.26 -0.4 4,521,200 - -1.0 4,521,221 + Pf1N1B4_4066 0.40 -0.3 4,521,222 - Pf1N1B4_4066 0.40 -1.3 4,521,222 - Pf1N1B4_4066 0.40 +0.9 4,521,479 + Pf1N1B4_4066 0.65 +0.6 4,521,662 + +2.2 4,521,663 - Pf1N1B4_4066 0.83 +0.8 4,521,788 + +0.6 4,521,817 + +0.0 4,521,868 + -0.0 4,521,911 - +0.6 4,522,041 + +0.6 4,522,041 + Pf1N1B4_4067 0.12 -0.2 4,522,041 + Pf1N1B4_4067 0.12 -1.4 4,522,042 - Pf1N1B4_4067 0.12 -0.4 4,522,042 - Pf1N1B4_4067 0.12 -2.2 4,522,167 - Pf1N1B4_4067 0.25 -0.6 4,522,167 - Pf1N1B4_4067 0.25 -0.4 4,522,628 + Pf1N1B4_4067 0.71 -1.5 4,522,629 - Pf1N1B4_4067 0.71 -1.1 4,522,911 - +2.2
Or see this region's nucleotide sequence