Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4046 and Pf1N1B4_4047 are separated by 127 nucleotides Pf1N1B4_4047 and Pf1N1B4_4048 overlap by 4 nucleotides Pf1N1B4_4048 and Pf1N1B4_4049 are separated by 59 nucleotides Pf1N1B4_4049 and Pf1N1B4_4050 are separated by 213 nucleotides Pf1N1B4_4050 and Pf1N1B4_4051 are separated by 38 nucleotides
Pf1N1B4_4046 - Adenylosuccinate lyase (EC 4.3.2.2), at 4,498,338 to 4,499,708
_4046
Pf1N1B4_4047 - FIG002903: a protein of unknown function perhaps involved in purine metabolism, at 4,499,836 to 4,500,459
_4047
Pf1N1B4_4048 - tRNA-specific 2-thiouridylase MnmA, at 4,500,456 to 4,501,589
_4048
Pf1N1B4_4049 - Nudix-like NDP and NTP phosphohydrolase YmfB, at 4,501,649 to 4,502,095
_4049
Pf1N1B4_4050 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,309 to 4,502,422
_4050
Pf1N1B4_4051 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,461 to 4,504,533
_4051
Position (kb)
4500
4501
4502 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4499.795 kb on - strand at 4499.795 kb on - strand at 4499.795 kb on - strand at 4499.811 kb on + strand at 4499.821 kb on + strand at 4499.868 kb on + strand at 4499.950 kb on + strand at 4499.954 kb on - strand, within Pf1N1B4_4047 at 4499.954 kb on - strand, within Pf1N1B4_4047 at 4499.954 kb on - strand, within Pf1N1B4_4047 at 4500.053 kb on + strand, within Pf1N1B4_4047 at 4500.053 kb on + strand, within Pf1N1B4_4047 at 4500.054 kb on - strand, within Pf1N1B4_4047 at 4500.054 kb on - strand, within Pf1N1B4_4047 at 4500.054 kb on - strand, within Pf1N1B4_4047 at 4500.299 kb on + strand, within Pf1N1B4_4047 at 4500.299 kb on + strand, within Pf1N1B4_4047 at 4500.299 kb on + strand, within Pf1N1B4_4047 at 4500.300 kb on - strand, within Pf1N1B4_4047 at 4500.300 kb on - strand, within Pf1N1B4_4047 at 4501.709 kb on - strand at 4501.709 kb on - strand, within Pf1N1B4_4049 at 4501.806 kb on + strand, within Pf1N1B4_4049 at 4501.854 kb on + strand, within Pf1N1B4_4049 at 4501.854 kb on + strand, within Pf1N1B4_4049 at 4501.855 kb on - strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4502.239 kb on + strand at 4502.239 kb on + strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.248 kb on - strand at 4502.248 kb on - strand at 4502.302 kb on + strand at 4502.310 kb on + strand at 4502.551 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 4,499,795 - +0.6 4,499,795 - -0.7 4,499,795 - +1.6 4,499,811 + +0.0 4,499,821 + +1.0 4,499,868 + +0.6 4,499,950 + -0.4 4,499,954 - Pf1N1B4_4047 0.19 +0.6 4,499,954 - Pf1N1B4_4047 0.19 +0.1 4,499,954 - Pf1N1B4_4047 0.19 -2.7 4,500,053 + Pf1N1B4_4047 0.35 -1.2 4,500,053 + Pf1N1B4_4047 0.35 +2.2 4,500,054 - Pf1N1B4_4047 0.35 -0.7 4,500,054 - Pf1N1B4_4047 0.35 -1.2 4,500,054 - Pf1N1B4_4047 0.35 -1.1 4,500,299 + Pf1N1B4_4047 0.74 -2.0 4,500,299 + Pf1N1B4_4047 0.74 +0.2 4,500,299 + Pf1N1B4_4047 0.74 -1.2 4,500,300 - Pf1N1B4_4047 0.74 -0.8 4,500,300 - Pf1N1B4_4047 0.74 -0.9 4,501,709 - +1.6 4,501,709 - Pf1N1B4_4049 0.13 -0.5 4,501,806 + Pf1N1B4_4049 0.35 -0.4 4,501,854 + Pf1N1B4_4049 0.46 +0.7 4,501,854 + Pf1N1B4_4049 0.46 +0.9 4,501,855 - Pf1N1B4_4049 0.46 +0.9 4,501,881 + Pf1N1B4_4049 0.52 -0.4 4,501,881 + Pf1N1B4_4049 0.52 -1.4 4,501,881 + Pf1N1B4_4049 0.52 +0.0 4,501,887 + +0.6 4,501,887 + Pf1N1B4_4049 0.53 +1.0 4,501,887 + Pf1N1B4_4049 0.53 -1.1 4,501,887 + Pf1N1B4_4049 0.53 -1.2 4,501,887 + Pf1N1B4_4049 0.53 -0.6 4,502,239 + -0.1 4,502,239 + +0.1 4,502,240 - -2.5 4,502,240 - -0.2 4,502,240 - +0.6 4,502,240 - -2.2 4,502,240 - -0.5 4,502,240 - +0.6 4,502,240 - +1.2 4,502,240 - -0.5 4,502,240 - -0.5 4,502,240 - -0.4 4,502,240 - +0.6 4,502,243 + +0.5 4,502,243 + +0.6 4,502,243 + -0.7 4,502,243 + -1.0 4,502,243 + +0.6 4,502,243 + +0.6 4,502,243 + -1.1 4,502,243 + -1.0 4,502,243 + +0.3 4,502,243 + -1.0 4,502,243 + -0.4 4,502,243 + +0.9 4,502,243 + +0.6 4,502,243 + -0.7 4,502,244 - -0.5 4,502,244 - +0.1 4,502,244 - +0.0 4,502,244 - +0.6 4,502,244 - +0.3 4,502,247 + +0.3 4,502,247 + +0.9 4,502,247 + -1.2 4,502,247 + +0.0 4,502,248 - -1.0 4,502,248 - +0.5 4,502,302 + -1.0 4,502,310 + -0.7 4,502,551 - -1.0
Or see this region's nucleotide sequence