Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4039 and Pf1N1B4_4040 are separated by 72 nucleotides Pf1N1B4_4040 and Pf1N1B4_4041 are separated by 10 nucleotides
Pf1N1B4_4039 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3), at 4,490,237 to 4,492,021
_4039
Pf1N1B4_4040 - NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3), at 4,492,094 to 4,492,768
_4040
Pf1N1B4_4041 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 4,492,779 to 4,493,192
_4041
Position (kb)
4492
4493 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4491.377 kb on + strand, within Pf1N1B4_4039 at 4491.377 kb on + strand, within Pf1N1B4_4039 at 4491.378 kb on - strand, within Pf1N1B4_4039 at 4491.378 kb on - strand, within Pf1N1B4_4039 at 4491.393 kb on + strand, within Pf1N1B4_4039 at 4491.393 kb on + strand, within Pf1N1B4_4039 at 4491.604 kb on - strand, within Pf1N1B4_4039 at 4491.628 kb on - strand, within Pf1N1B4_4039 at 4491.704 kb on + strand, within Pf1N1B4_4039 at 4491.704 kb on + strand, within Pf1N1B4_4039 at 4491.705 kb on - strand, within Pf1N1B4_4039 at 4491.713 kb on - strand, within Pf1N1B4_4039 at 4491.713 kb on - strand, within Pf1N1B4_4039 at 4491.716 kb on - strand, within Pf1N1B4_4039 at 4491.739 kb on - strand, within Pf1N1B4_4039 at 4491.816 kb on + strand, within Pf1N1B4_4039 at 4491.817 kb on - strand, within Pf1N1B4_4039 at 4491.817 kb on - strand, within Pf1N1B4_4039 at 4491.817 kb on - strand, within Pf1N1B4_4039 at 4491.828 kb on + strand, within Pf1N1B4_4039 at 4491.829 kb on - strand, within Pf1N1B4_4039 at 4491.987 kb on + strand at 4491.988 kb on - strand at 4492.000 kb on - strand at 4492.000 kb on - strand at 4492.025 kb on + strand at 4492.025 kb on + strand at 4492.026 kb on - strand at 4492.026 kb on - strand at 4492.026 kb on - strand at 4492.026 kb on - strand at 4492.048 kb on - strand at 4492.048 kb on - strand at 4492.048 kb on - strand at 4492.048 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.133 kb on - strand at 4492.296 kb on + strand, within Pf1N1B4_4040 at 4492.297 kb on - strand, within Pf1N1B4_4040 at 4492.350 kb on + strand, within Pf1N1B4_4040 at 4492.351 kb on - strand, within Pf1N1B4_4040 at 4492.396 kb on + strand at 4492.563 kb on + strand, within Pf1N1B4_4040 at 4492.585 kb on + strand, within Pf1N1B4_4040 at 4492.586 kb on - strand, within Pf1N1B4_4040 at 4492.761 kb on + strand at 4492.762 kb on - strand at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.879 kb on - strand, within Pf1N1B4_4041 at 4492.994 kb on - strand, within Pf1N1B4_4041 at 4492.994 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.284 kb on + strand at 4493.326 kb on - strand at 4493.329 kb on + strand at 4493.330 kb on - strand at 4493.345 kb on + strand at 4493.406 kb on + strand at 4493.406 kb on + strand at 4493.406 kb on + strand at 4493.425 kb on - strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.551 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 4,491,377 + Pf1N1B4_4039 0.64 +1.4 4,491,377 + Pf1N1B4_4039 0.64 +0.6 4,491,378 - Pf1N1B4_4039 0.64 -0.5 4,491,378 - Pf1N1B4_4039 0.64 -0.4 4,491,393 + Pf1N1B4_4039 0.65 -0.4 4,491,393 + Pf1N1B4_4039 0.65 -0.1 4,491,604 - Pf1N1B4_4039 0.77 +1.6 4,491,628 - Pf1N1B4_4039 0.78 +0.2 4,491,704 + Pf1N1B4_4039 0.82 +1.7 4,491,704 + Pf1N1B4_4039 0.82 +2.8 4,491,705 - Pf1N1B4_4039 0.82 +1.2 4,491,713 - Pf1N1B4_4039 0.83 +1.9 4,491,713 - Pf1N1B4_4039 0.83 -0.2 4,491,716 - Pf1N1B4_4039 0.83 +0.8 4,491,739 - Pf1N1B4_4039 0.84 +0.3 4,491,816 + Pf1N1B4_4039 0.88 -2.5 4,491,817 - Pf1N1B4_4039 0.89 -0.5 4,491,817 - Pf1N1B4_4039 0.89 +0.1 4,491,817 - Pf1N1B4_4039 0.89 -0.4 4,491,828 + Pf1N1B4_4039 0.89 -0.1 4,491,829 - Pf1N1B4_4039 0.89 +0.3 4,491,987 + -0.4 4,491,988 - +0.2 4,492,000 - +0.6 4,492,000 - +0.2 4,492,025 + +1.3 4,492,025 + -0.1 4,492,026 - -1.2 4,492,026 - +0.3 4,492,026 - -0.1 4,492,026 - +0.6 4,492,048 - -0.3 4,492,048 - +2.2 4,492,048 - -0.4 4,492,048 - -0.4 4,492,079 - -0.4 4,492,079 - +0.0 4,492,079 - +0.6 4,492,079 - +0.2 4,492,079 - -1.0 4,492,133 - -0.1 4,492,296 + Pf1N1B4_4040 0.30 +0.6 4,492,297 - Pf1N1B4_4040 0.30 +0.6 4,492,350 + Pf1N1B4_4040 0.38 +0.4 4,492,351 - Pf1N1B4_4040 0.38 -0.2 4,492,396 + +1.9 4,492,563 + Pf1N1B4_4040 0.69 -1.2 4,492,585 + Pf1N1B4_4040 0.73 +1.6 4,492,586 - Pf1N1B4_4040 0.73 +0.4 4,492,761 + +1.2 4,492,762 - +1.5 4,492,853 - Pf1N1B4_4041 0.18 -0.5 4,492,853 - Pf1N1B4_4041 0.18 -1.3 4,492,853 - Pf1N1B4_4041 0.18 +0.9 4,492,879 - Pf1N1B4_4041 0.24 +2.1 4,492,994 - Pf1N1B4_4041 0.52 +0.6 4,492,994 - Pf1N1B4_4041 0.52 +0.6 4,493,035 - Pf1N1B4_4041 0.62 -0.2 4,493,035 - Pf1N1B4_4041 0.62 +0.6 4,493,035 - Pf1N1B4_4041 0.62 +1.6 4,493,284 + +1.6 4,493,326 - +1.2 4,493,329 + -0.8 4,493,330 - -1.7 4,493,345 + -0.4 4,493,406 + -0.8 4,493,406 + +0.3 4,493,406 + -1.0 4,493,425 - -0.4 4,493,433 + +2.2 4,493,433 + -0.2 4,493,433 + +1.1 4,493,433 + -0.6 4,493,433 + +1.8 4,493,433 + +1.6 4,493,433 + -0.5 4,493,433 + +0.6 4,493,433 + +1.6 4,493,433 + +0.8 4,493,433 + +0.1 4,493,434 - +1.3 4,493,434 - -0.4 4,493,434 - -0.2 4,493,434 - +0.3 4,493,434 - +0.6 4,493,513 + +0.6 4,493,513 + +0.3 4,493,513 + +0.2 4,493,513 + +0.1 4,493,514 - -0.2 4,493,514 - -0.4 4,493,514 - +1.2 4,493,514 - +0.6 4,493,514 - -0.7 4,493,515 + +2.4 4,493,515 + -1.7 4,493,515 + -0.4 4,493,515 + -0.4 4,493,515 + +1.6 4,493,516 - -0.8 4,493,516 - -0.1 4,493,516 - +0.6 4,493,516 - -0.7 4,493,551 + -0.8
Or see this region's nucleotide sequence