Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3572

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3571 and Pf1N1B4_3572 are separated by 11 nucleotidesPf1N1B4_3572 and Pf1N1B4_3573 are separated by 23 nucleotides Pf1N1B4_3571 - Flagellar P-ring protein FlgI, at 3,974,130 to 3,975,239 _3571 Pf1N1B4_3572 - Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-), at 3,975,251 to 3,976,549 _3572 Pf1N1B4_3573 - Flagellar hook-associated protein FlgK, at 3,976,573 to 3,978,633 _3573 Position (kb) 3975 3976 3977Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3974.273 kb on + strand, within Pf1N1B4_3571at 3974.449 kb on + strand, within Pf1N1B4_3571at 3974.449 kb on + strand, within Pf1N1B4_3571at 3974.449 kb on + strand, within Pf1N1B4_3571at 3974.449 kb on + strand, within Pf1N1B4_3571at 3974.449 kb on + strand, within Pf1N1B4_3571at 3974.449 kb on + strand, within Pf1N1B4_3571at 3974.449 kb on + strand, within Pf1N1B4_3571at 3974.449 kb on + strand, within Pf1N1B4_3571at 3974.449 kb on + strand, within Pf1N1B4_3571at 3974.449 kb on + strand, within Pf1N1B4_3571at 3974.449 kb on + strand, within Pf1N1B4_3571at 3974.450 kb on - strand, within Pf1N1B4_3571at 3974.450 kb on - strand, within Pf1N1B4_3571at 3974.450 kb on - strand, within Pf1N1B4_3571at 3974.450 kb on - strand, within Pf1N1B4_3571at 3974.450 kb on - strand, within Pf1N1B4_3571at 3974.450 kb on - strand, within Pf1N1B4_3571at 3974.450 kb on - strand, within Pf1N1B4_3571at 3974.454 kb on - strand, within Pf1N1B4_3571at 3974.454 kb on - strandat 3974.542 kb on - strand, within Pf1N1B4_3571at 3974.542 kb on - strand, within Pf1N1B4_3571at 3974.847 kb on + strand, within Pf1N1B4_3571at 3974.847 kb on + strandat 3974.848 kb on - strandat 3974.989 kb on + strand, within Pf1N1B4_3571at 3975.256 kb on + strandat 3975.299 kb on + strandat 3975.300 kb on - strandat 3975.491 kb on + strand, within Pf1N1B4_3572at 3975.492 kb on - strand, within Pf1N1B4_3572at 3975.492 kb on - strand, within Pf1N1B4_3572at 3975.941 kb on + strand, within Pf1N1B4_3572at 3975.942 kb on - strand, within Pf1N1B4_3572at 3976.002 kb on - strand, within Pf1N1B4_3572at 3976.024 kb on - strandat 3976.131 kb on - strand, within Pf1N1B4_3572at 3976.131 kb on - strand, within Pf1N1B4_3572at 3976.131 kb on - strand, within Pf1N1B4_3572at 3976.234 kb on + strand, within Pf1N1B4_3572at 3976.234 kb on + strand, within Pf1N1B4_3572at 3976.329 kb on - strand, within Pf1N1B4_3572at 3976.329 kb on - strand, within Pf1N1B4_3572at 3976.373 kb on - strand, within Pf1N1B4_3572at 3976.373 kb on - strand, within Pf1N1B4_3572at 3976.537 kb on + strandat 3976.537 kb on + strandat 3976.545 kb on + strandat 3976.641 kb on + strandat 3976.642 kb on - strandat 3976.726 kb on + strandat 3976.727 kb on - strandat 3976.727 kb on - strandat 3976.769 kb on - strandat 3976.777 kb on + strandat 3976.858 kb on - strand, within Pf1N1B4_3573at 3977.080 kb on - strand, within Pf1N1B4_3573at 3977.242 kb on + strand, within Pf1N1B4_3573

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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3,974,273 + Pf1N1B4_3571 0.13 +0.2
3,974,449 + Pf1N1B4_3571 0.29 +0.1
3,974,449 + Pf1N1B4_3571 0.29 -0.5
3,974,449 + Pf1N1B4_3571 0.29 -0.8
3,974,449 + Pf1N1B4_3571 0.29 -0.2
3,974,449 + Pf1N1B4_3571 0.29 -0.2
3,974,449 + Pf1N1B4_3571 0.29 -1.0
3,974,449 + Pf1N1B4_3571 0.29 +2.4
3,974,449 + Pf1N1B4_3571 0.29 -1.0
3,974,449 + Pf1N1B4_3571 0.29 +0.6
3,974,449 + Pf1N1B4_3571 0.29 +0.2
3,974,449 + Pf1N1B4_3571 0.29 -1.4
3,974,450 - Pf1N1B4_3571 0.29 +0.1
3,974,450 - Pf1N1B4_3571 0.29 -0.3
3,974,450 - Pf1N1B4_3571 0.29 -0.0
3,974,450 - Pf1N1B4_3571 0.29 -0.5
3,974,450 - Pf1N1B4_3571 0.29 -0.4
3,974,450 - Pf1N1B4_3571 0.29 -2.5
3,974,450 - Pf1N1B4_3571 0.29 -0.5
3,974,454 - Pf1N1B4_3571 0.29 -0.0
3,974,454 - +0.8
3,974,542 - Pf1N1B4_3571 0.37 +2.6
3,974,542 - Pf1N1B4_3571 0.37 -1.6
3,974,847 + Pf1N1B4_3571 0.65 -1.2
3,974,847 + +0.8
3,974,848 - +2.8
3,974,989 + Pf1N1B4_3571 0.77 -1.2
3,975,256 + +0.4
3,975,299 + +0.4
3,975,300 - -0.5
3,975,491 + Pf1N1B4_3572 0.18 -0.5
3,975,492 - Pf1N1B4_3572 0.19 +0.4
3,975,492 - Pf1N1B4_3572 0.19 -1.2
3,975,941 + Pf1N1B4_3572 0.53 -0.4
3,975,942 - Pf1N1B4_3572 0.53 -2.0
3,976,002 - Pf1N1B4_3572 0.58 -0.8
3,976,024 - +1.8
3,976,131 - Pf1N1B4_3572 0.68 -0.5
3,976,131 - Pf1N1B4_3572 0.68 -0.4
3,976,131 - Pf1N1B4_3572 0.68 -2.7
3,976,234 + Pf1N1B4_3572 0.76 +0.2
3,976,234 + Pf1N1B4_3572 0.76 -2.0
3,976,329 - Pf1N1B4_3572 0.83 +0.8
3,976,329 - Pf1N1B4_3572 0.83 -0.8
3,976,373 - Pf1N1B4_3572 0.86 -2.7
3,976,373 - Pf1N1B4_3572 0.86 -1.1
3,976,537 + -0.6
3,976,537 + -3.0
3,976,545 + +1.1
3,976,641 + +1.8
3,976,642 - +0.5
3,976,726 + -0.6
3,976,727 - -0.2
3,976,727 - -0.2
3,976,769 - -0.2
3,976,777 + -0.2
3,976,858 - Pf1N1B4_3573 0.14 -0.5
3,977,080 - Pf1N1B4_3573 0.25 -0.8
3,977,242 + Pf1N1B4_3573 0.32 -0.6

Or see this region's nucleotide sequence