Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3380 and Pf1N1B4_3381 overlap by 8 nucleotides Pf1N1B4_3381 and Pf1N1B4_3382 overlap by 4 nucleotides Pf1N1B4_3382 and Pf1N1B4_3383 are separated by 521 nucleotides
Pf1N1B4_3380 - D-glucarate permease, at 3,767,934 to 3,769,205
_3380
Pf1N1B4_3381 - Y4mH, at 3,769,198 to 3,770,154
_3381
Pf1N1B4_3382 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 3,770,151 to 3,771,155
_3382
Pf1N1B4_3383 - Mandelate racemase (EC 5.1.2.2), at 3,771,677 to 3,772,852
_3383
Position (kb)
3770
3771
3772 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3769.171 kb on + strand at 3769.171 kb on + strand at 3769.172 kb on - strand at 3769.337 kb on - strand, within Pf1N1B4_3381 at 3769.351 kb on + strand, within Pf1N1B4_3381 at 3769.351 kb on + strand, within Pf1N1B4_3381 at 3769.351 kb on + strand, within Pf1N1B4_3381 at 3769.352 kb on - strand, within Pf1N1B4_3381 at 3769.393 kb on + strand, within Pf1N1B4_3381 at 3769.393 kb on + strand, within Pf1N1B4_3381 at 3769.393 kb on + strand, within Pf1N1B4_3381 at 3769.393 kb on + strand at 3769.393 kb on + strand, within Pf1N1B4_3381 at 3769.393 kb on + strand, within Pf1N1B4_3381 at 3769.393 kb on + strand, within Pf1N1B4_3381 at 3769.394 kb on - strand, within Pf1N1B4_3381 at 3769.394 kb on - strand, within Pf1N1B4_3381 at 3769.394 kb on - strand, within Pf1N1B4_3381 at 3769.394 kb on - strand, within Pf1N1B4_3381 at 3769.394 kb on - strand, within Pf1N1B4_3381 at 3769.394 kb on - strand, within Pf1N1B4_3381 at 3770.012 kb on - strand, within Pf1N1B4_3381 at 3770.012 kb on - strand, within Pf1N1B4_3381 at 3770.092 kb on + strand at 3770.379 kb on + strand, within Pf1N1B4_3382 at 3770.380 kb on - strand, within Pf1N1B4_3382 at 3770.587 kb on + strand, within Pf1N1B4_3382 at 3770.588 kb on - strand at 3770.834 kb on + strand, within Pf1N1B4_3382 at 3770.958 kb on + strand, within Pf1N1B4_3382 at 3770.958 kb on + strand, within Pf1N1B4_3382 at 3770.958 kb on + strand, within Pf1N1B4_3382 at 3770.958 kb on + strand, within Pf1N1B4_3382 at 3770.958 kb on + strand, within Pf1N1B4_3382 at 3770.958 kb on + strand, within Pf1N1B4_3382 at 3770.959 kb on - strand, within Pf1N1B4_3382 at 3770.959 kb on - strand, within Pf1N1B4_3382 at 3770.959 kb on - strand, within Pf1N1B4_3382 at 3770.959 kb on - strand, within Pf1N1B4_3382 at 3770.959 kb on - strand at 3770.959 kb on - strand, within Pf1N1B4_3382 at 3771.150 kb on + strand at 3771.150 kb on + strand at 3771.150 kb on + strand at 3771.150 kb on + strand at 3771.151 kb on - strand at 3771.151 kb on - strand at 3771.151 kb on - strand at 3771.151 kb on - strand at 3771.151 kb on - strand at 3771.223 kb on + strand at 3771.224 kb on - strand at 3771.257 kb on - strand at 3771.257 kb on - strand at 3771.264 kb on - strand at 3771.270 kb on - strand at 3771.270 kb on - strand at 3771.287 kb on + strand at 3771.548 kb on + strand at 3771.548 kb on + strand at 3771.548 kb on + strand at 3771.549 kb on - strand at 3771.549 kb on - strand at 3771.549 kb on - strand at 3771.549 kb on - strand at 3771.549 kb on - strand at 3771.645 kb on - strand at 3771.645 kb on - strand at 3771.645 kb on - strand at 3771.672 kb on + strand at 3771.672 kb on + strand at 3771.673 kb on - strand at 3771.673 kb on - strand at 3771.673 kb on - strand at 3771.909 kb on - strand, within Pf1N1B4_3383 at 3771.953 kb on + strand, within Pf1N1B4_3383 at 3771.954 kb on - strand, within Pf1N1B4_3383 at 3771.954 kb on - strand, within Pf1N1B4_3383 at 3771.954 kb on - strand, within Pf1N1B4_3383 at 3771.954 kb on - strand, within Pf1N1B4_3383 at 3771.954 kb on - strand, within Pf1N1B4_3383 at 3771.971 kb on + strand, within Pf1N1B4_3383 at 3771.972 kb on - strand, within Pf1N1B4_3383 at 3772.121 kb on + strand, within Pf1N1B4_3383 at 3772.122 kb on - strand, within Pf1N1B4_3383 at 3772.122 kb on - strand, within Pf1N1B4_3383
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 3,769,171 + -0.4 3,769,171 + -0.4 3,769,172 - -1.7 3,769,337 - Pf1N1B4_3381 0.15 -0.6 3,769,351 + Pf1N1B4_3381 0.16 -0.8 3,769,351 + Pf1N1B4_3381 0.16 -0.2 3,769,351 + Pf1N1B4_3381 0.16 +0.2 3,769,352 - Pf1N1B4_3381 0.16 +1.5 3,769,393 + Pf1N1B4_3381 0.20 -0.1 3,769,393 + Pf1N1B4_3381 0.20 -0.8 3,769,393 + Pf1N1B4_3381 0.20 -0.3 3,769,393 + +0.9 3,769,393 + Pf1N1B4_3381 0.20 -0.7 3,769,393 + Pf1N1B4_3381 0.20 +1.6 3,769,393 + Pf1N1B4_3381 0.20 +0.9 3,769,394 - Pf1N1B4_3381 0.20 -0.1 3,769,394 - Pf1N1B4_3381 0.20 +0.5 3,769,394 - Pf1N1B4_3381 0.20 +0.9 3,769,394 - Pf1N1B4_3381 0.20 +0.6 3,769,394 - Pf1N1B4_3381 0.20 -0.7 3,769,394 - Pf1N1B4_3381 0.20 +0.9 3,770,012 - Pf1N1B4_3381 0.85 +1.7 3,770,012 - Pf1N1B4_3381 0.85 -0.9 3,770,092 + +0.9 3,770,379 + Pf1N1B4_3382 0.23 -2.1 3,770,380 - Pf1N1B4_3382 0.23 +0.9 3,770,587 + Pf1N1B4_3382 0.43 -0.1 3,770,588 - +0.9 3,770,834 + Pf1N1B4_3382 0.68 -1.2 3,770,958 + Pf1N1B4_3382 0.80 +0.6 3,770,958 + Pf1N1B4_3382 0.80 -2.8 3,770,958 + Pf1N1B4_3382 0.80 -1.1 3,770,958 + Pf1N1B4_3382 0.80 -2.1 3,770,958 + Pf1N1B4_3382 0.80 -2.3 3,770,958 + Pf1N1B4_3382 0.80 +0.6 3,770,959 - Pf1N1B4_3382 0.80 -1.9 3,770,959 - Pf1N1B4_3382 0.80 -0.0 3,770,959 - Pf1N1B4_3382 0.80 +0.7 3,770,959 - Pf1N1B4_3382 0.80 -1.1 3,770,959 - -0.1 3,770,959 - Pf1N1B4_3382 0.80 -2.1 3,771,150 + +1.3 3,771,150 + -1.8 3,771,150 + +0.6 3,771,150 + -0.7 3,771,151 - -0.3 3,771,151 - +1.9 3,771,151 - -0.7 3,771,151 - +0.1 3,771,151 - -0.4 3,771,223 + -0.3 3,771,224 - -0.2 3,771,257 - +0.6 3,771,257 - +0.9 3,771,264 - -1.3 3,771,270 - -0.6 3,771,270 - +0.5 3,771,287 + -0.4 3,771,548 + -1.9 3,771,548 + -0.1 3,771,548 + +0.5 3,771,549 - +0.9 3,771,549 - +0.3 3,771,549 - +0.2 3,771,549 - -0.7 3,771,549 - -1.3 3,771,645 - -1.4 3,771,645 - -1.0 3,771,645 - +0.3 3,771,672 + -0.4 3,771,672 + -0.0 3,771,673 - +0.7 3,771,673 - +1.6 3,771,673 - -0.7 3,771,909 - Pf1N1B4_3383 0.20 -1.4 3,771,953 + Pf1N1B4_3383 0.23 +1.5 3,771,954 - Pf1N1B4_3383 0.24 -1.4 3,771,954 - Pf1N1B4_3383 0.24 +1.1 3,771,954 - Pf1N1B4_3383 0.24 -1.1 3,771,954 - Pf1N1B4_3383 0.24 -1.4 3,771,954 - Pf1N1B4_3383 0.24 +0.1 3,771,971 + Pf1N1B4_3383 0.25 -2.4 3,771,972 - Pf1N1B4_3383 0.25 +0.3 3,772,121 + Pf1N1B4_3383 0.38 -1.1 3,772,122 - Pf1N1B4_3383 0.38 +1.5 3,772,122 - Pf1N1B4_3383 0.38 +0.9
Or see this region's nucleotide sequence