Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_336 and Pf1N1B4_337 overlap by 11 nucleotides Pf1N1B4_337 and Pf1N1B4_338 are separated by 117 nucleotides
Pf1N1B4_336 - Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40), at 399,494 to 400,861
_336
Pf1N1B4_337 - O-antigen export system permease protein RfbD, at 400,851 to 401,669
_337
Pf1N1B4_338 - Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-), at 401,787 to 402,794
_338
Position (kb) 400
401
402 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 399.852 kb on + strand, within Pf1N1B4_336 at 399.853 kb on - strand, within Pf1N1B4_336 at 399.853 kb on - strand, within Pf1N1B4_336 at 399.853 kb on - strand, within Pf1N1B4_336 at 399.856 kb on + strand, within Pf1N1B4_336 at 399.856 kb on + strand, within Pf1N1B4_336 at 399.856 kb on + strand at 399.856 kb on + strand, within Pf1N1B4_336 at 399.856 kb on + strand, within Pf1N1B4_336 at 399.856 kb on + strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.857 kb on - strand, within Pf1N1B4_336 at 399.858 kb on + strand, within Pf1N1B4_336 at 399.859 kb on - strand, within Pf1N1B4_336 at 399.859 kb on - strand, within Pf1N1B4_336 at 399.864 kb on + strand, within Pf1N1B4_336 at 399.864 kb on + strand, within Pf1N1B4_336 at 399.865 kb on - strand, within Pf1N1B4_336 at 399.865 kb on - strand, within Pf1N1B4_336 at 399.865 kb on - strand, within Pf1N1B4_336 at 399.865 kb on - strand, within Pf1N1B4_336 at 399.865 kb on - strand, within Pf1N1B4_336 at 399.958 kb on + strand, within Pf1N1B4_336 at 399.959 kb on - strand, within Pf1N1B4_336 at 399.959 kb on - strand, within Pf1N1B4_336 at 399.976 kb on + strand, within Pf1N1B4_336 at 399.977 kb on - strand, within Pf1N1B4_336 at 399.977 kb on - strand, within Pf1N1B4_336 at 399.977 kb on - strand, within Pf1N1B4_336 at 400.023 kb on - strand, within Pf1N1B4_336 at 400.131 kb on + strand, within Pf1N1B4_336 at 400.131 kb on + strand, within Pf1N1B4_336 at 400.131 kb on + strand, within Pf1N1B4_336 at 400.131 kb on + strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.132 kb on - strand, within Pf1N1B4_336 at 400.146 kb on + strand, within Pf1N1B4_336 at 400.262 kb on - strand, within Pf1N1B4_336 at 400.279 kb on + strand, within Pf1N1B4_336 at 400.316 kb on + strand, within Pf1N1B4_336 at 400.316 kb on + strand, within Pf1N1B4_336 at 400.317 kb on - strand, within Pf1N1B4_336 at 400.317 kb on - strand, within Pf1N1B4_336 at 400.355 kb on + strand, within Pf1N1B4_336 at 400.356 kb on - strand, within Pf1N1B4_336 at 400.356 kb on - strand, within Pf1N1B4_336 at 400.412 kb on - strand, within Pf1N1B4_336 at 400.412 kb on - strand, within Pf1N1B4_336 at 400.417 kb on - strand, within Pf1N1B4_336 at 400.417 kb on - strand, within Pf1N1B4_336 at 400.417 kb on - strand, within Pf1N1B4_336 at 400.417 kb on - strand, within Pf1N1B4_336 at 400.527 kb on + strand, within Pf1N1B4_336 at 400.528 kb on - strand, within Pf1N1B4_336 at 400.528 kb on - strand, within Pf1N1B4_336 at 400.752 kb on + strand at 400.752 kb on + strand at 400.752 kb on + strand at 400.752 kb on + strand at 400.806 kb on + strand at 400.807 kb on - strand at 400.807 kb on - strand at 400.810 kb on + strand at 400.810 kb on + strand at 400.811 kb on - strand at 400.849 kb on + strand at 400.850 kb on - strand at 400.850 kb on - strand at 400.850 kb on - strand at 400.850 kb on - strand at 400.850 kb on - strand at 400.852 kb on + strand at 400.874 kb on + strand at 400.928 kb on + strand at 400.929 kb on - strand at 400.930 kb on + strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.933 kb on + strand, within Pf1N1B4_337 at 400.933 kb on + strand, within Pf1N1B4_337 at 400.934 kb on - strand, within Pf1N1B4_337 at 400.934 kb on - strand at 400.934 kb on - strand, within Pf1N1B4_337 at 400.934 kb on - strand, within Pf1N1B4_337 at 400.960 kb on - strand, within Pf1N1B4_337 at 400.960 kb on - strand, within Pf1N1B4_337 at 401.216 kb on + strand, within Pf1N1B4_337 at 401.217 kb on - strand, within Pf1N1B4_337 at 401.217 kb on - strand, within Pf1N1B4_337 at 401.264 kb on + strand, within Pf1N1B4_337 at 401.316 kb on + strand, within Pf1N1B4_337 at 401.344 kb on - strand, within Pf1N1B4_337 at 401.386 kb on - strand, within Pf1N1B4_337 at 401.405 kb on + strand, within Pf1N1B4_337 at 401.406 kb on - strand, within Pf1N1B4_337 at 401.406 kb on - strand, within Pf1N1B4_337 at 401.432 kb on + strand, within Pf1N1B4_337 at 401.432 kb on + strand, within Pf1N1B4_337 at 401.655 kb on - strand at 401.675 kb on - strand at 401.735 kb on + strand at 402.084 kb on - strand, within Pf1N1B4_338 at 402.086 kb on - strand, within Pf1N1B4_338 at 402.086 kb on - strand, within Pf1N1B4_338 at 402.145 kb on + strand, within Pf1N1B4_338 at 402.198 kb on + strand, within Pf1N1B4_338 at 402.443 kb on - strand, within Pf1N1B4_338 at 402.474 kb on - strand at 402.554 kb on - strand, within Pf1N1B4_338
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 399,852 + Pf1N1B4_336 0.26 +1.1 399,853 - Pf1N1B4_336 0.26 +1.6 399,853 - Pf1N1B4_336 0.26 +1.1 399,853 - Pf1N1B4_336 0.26 -1.2 399,856 + Pf1N1B4_336 0.26 +1.6 399,856 + Pf1N1B4_336 0.26 -0.4 399,856 + +0.8 399,856 + Pf1N1B4_336 0.26 +0.4 399,856 + Pf1N1B4_336 0.26 +1.0 399,856 + Pf1N1B4_336 0.26 +0.1 399,857 - Pf1N1B4_336 0.27 -0.8 399,857 - Pf1N1B4_336 0.27 -1.8 399,857 - Pf1N1B4_336 0.27 -0.9 399,857 - Pf1N1B4_336 0.27 +0.8 399,857 - Pf1N1B4_336 0.27 -0.3 399,857 - Pf1N1B4_336 0.27 -0.0 399,857 - Pf1N1B4_336 0.27 -0.9 399,857 - Pf1N1B4_336 0.27 +0.0 399,857 - Pf1N1B4_336 0.27 -0.0 399,857 - Pf1N1B4_336 0.27 +0.5 399,857 - Pf1N1B4_336 0.27 +0.2 399,857 - Pf1N1B4_336 0.27 +1.1 399,857 - Pf1N1B4_336 0.27 +0.5 399,857 - Pf1N1B4_336 0.27 +0.4 399,857 - Pf1N1B4_336 0.27 -0.7 399,857 - Pf1N1B4_336 0.27 -0.7 399,857 - Pf1N1B4_336 0.27 +0.4 399,857 - Pf1N1B4_336 0.27 +0.5 399,857 - Pf1N1B4_336 0.27 -0.5 399,857 - Pf1N1B4_336 0.27 +0.5 399,857 - Pf1N1B4_336 0.27 +0.8 399,857 - Pf1N1B4_336 0.27 +0.2 399,857 - Pf1N1B4_336 0.27 +0.4 399,857 - Pf1N1B4_336 0.27 +0.3 399,857 - Pf1N1B4_336 0.27 -0.4 399,857 - Pf1N1B4_336 0.27 -1.6 399,857 - Pf1N1B4_336 0.27 -0.2 399,857 - Pf1N1B4_336 0.27 -0.3 399,857 - Pf1N1B4_336 0.27 +0.5 399,857 - Pf1N1B4_336 0.27 +0.4 399,858 + Pf1N1B4_336 0.27 -1.0 399,859 - Pf1N1B4_336 0.27 +1.4 399,859 - Pf1N1B4_336 0.27 +0.2 399,864 + Pf1N1B4_336 0.27 +1.8 399,864 + Pf1N1B4_336 0.27 +3.1 399,865 - Pf1N1B4_336 0.27 +1.2 399,865 - Pf1N1B4_336 0.27 +0.0 399,865 - Pf1N1B4_336 0.27 +0.1 399,865 - Pf1N1B4_336 0.27 +0.1 399,865 - Pf1N1B4_336 0.27 -1.0 399,958 + Pf1N1B4_336 0.34 -0.1 399,959 - Pf1N1B4_336 0.34 -1.6 399,959 - Pf1N1B4_336 0.34 +0.4 399,976 + Pf1N1B4_336 0.35 +0.2 399,977 - Pf1N1B4_336 0.35 +1.9 399,977 - Pf1N1B4_336 0.35 +0.5 399,977 - Pf1N1B4_336 0.35 +0.4 400,023 - Pf1N1B4_336 0.39 +0.5 400,131 + Pf1N1B4_336 0.47 +1.1 400,131 + Pf1N1B4_336 0.47 +0.9 400,131 + Pf1N1B4_336 0.47 +0.8 400,131 + Pf1N1B4_336 0.47 +0.8 400,132 - Pf1N1B4_336 0.47 -0.6 400,132 - Pf1N1B4_336 0.47 +0.0 400,132 - Pf1N1B4_336 0.47 +0.8 400,132 - Pf1N1B4_336 0.47 +1.7 400,132 - Pf1N1B4_336 0.47 -0.1 400,132 - Pf1N1B4_336 0.47 -0.1 400,132 - Pf1N1B4_336 0.47 +0.3 400,132 - Pf1N1B4_336 0.47 +2.1 400,132 - Pf1N1B4_336 0.47 -1.2 400,132 - Pf1N1B4_336 0.47 +0.4 400,132 - Pf1N1B4_336 0.47 +1.0 400,132 - Pf1N1B4_336 0.47 -0.8 400,132 - Pf1N1B4_336 0.47 -0.0 400,132 - Pf1N1B4_336 0.47 +0.2 400,132 - Pf1N1B4_336 0.47 -0.2 400,132 - Pf1N1B4_336 0.47 +1.1 400,146 + Pf1N1B4_336 0.48 +0.5 400,262 - Pf1N1B4_336 0.56 -0.2 400,279 + Pf1N1B4_336 0.57 -0.2 400,316 + Pf1N1B4_336 0.60 -0.0 400,316 + Pf1N1B4_336 0.60 -1.8 400,317 - Pf1N1B4_336 0.60 +0.8 400,317 - Pf1N1B4_336 0.60 +1.9 400,355 + Pf1N1B4_336 0.63 -0.4 400,356 - Pf1N1B4_336 0.63 +0.6 400,356 - Pf1N1B4_336 0.63 -0.3 400,412 - Pf1N1B4_336 0.67 +1.5 400,412 - Pf1N1B4_336 0.67 -0.4 400,417 - Pf1N1B4_336 0.67 +0.8 400,417 - Pf1N1B4_336 0.67 -0.9 400,417 - Pf1N1B4_336 0.67 +1.5 400,417 - Pf1N1B4_336 0.67 +0.1 400,527 + Pf1N1B4_336 0.76 +1.1 400,528 - Pf1N1B4_336 0.76 +0.8 400,528 - Pf1N1B4_336 0.76 -1.5 400,752 + +1.1 400,752 + -0.8 400,752 + +0.6 400,752 + +0.8 400,806 + +1.0 400,807 - -0.5 400,807 - +0.9 400,810 + +0.5 400,810 + +2.6 400,811 - -0.5 400,849 + +0.6 400,850 - +1.4 400,850 - -0.1 400,850 - +0.2 400,850 - +0.8 400,850 - +0.1 400,852 + -0.2 400,874 + +0.9 400,928 + +2.1 400,929 - -0.7 400,930 + +0.8 400,931 - -1.0 400,931 - +1.1 400,931 - +0.0 400,931 - -0.2 400,931 - -0.5 400,931 - +1.0 400,931 - +0.8 400,931 - +0.3 400,931 - -0.5 400,933 + Pf1N1B4_337 0.10 +1.1 400,933 + Pf1N1B4_337 0.10 +0.5 400,934 - Pf1N1B4_337 0.10 -0.8 400,934 - +0.8 400,934 - Pf1N1B4_337 0.10 +0.8 400,934 - Pf1N1B4_337 0.10 -1.0 400,960 - Pf1N1B4_337 0.13 -0.1 400,960 - Pf1N1B4_337 0.13 -2.5 401,216 + Pf1N1B4_337 0.45 +3.6 401,217 - Pf1N1B4_337 0.45 +0.9 401,217 - Pf1N1B4_337 0.45 -0.6 401,264 + Pf1N1B4_337 0.50 +0.8 401,316 + Pf1N1B4_337 0.57 +2.1 401,344 - Pf1N1B4_337 0.60 +0.8 401,386 - Pf1N1B4_337 0.65 -0.4 401,405 + Pf1N1B4_337 0.68 +1.8 401,406 - Pf1N1B4_337 0.68 +0.8 401,406 - Pf1N1B4_337 0.68 +0.9 401,432 + Pf1N1B4_337 0.71 +1.4 401,432 + Pf1N1B4_337 0.71 -0.8 401,655 - -0.6 401,675 - +0.8 401,735 + +2.1 402,084 - Pf1N1B4_338 0.29 +2.4 402,086 - Pf1N1B4_338 0.30 -1.2 402,086 - Pf1N1B4_338 0.30 -0.1 402,145 + Pf1N1B4_338 0.36 +0.3 402,198 + Pf1N1B4_338 0.41 -0.2 402,443 - Pf1N1B4_338 0.65 +2.1 402,474 - -0.2 402,554 - Pf1N1B4_338 0.76 +1.4
Or see this region's nucleotide sequence