Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3271 and Pf1N1B4_3272 are separated by 19 nucleotides Pf1N1B4_3272 and Pf1N1B4_3273 are separated by 79 nucleotides Pf1N1B4_3273 and Pf1N1B4_3274 are separated by 307 nucleotides
Pf1N1B4_3271 - Bicyclomycin resistance protein, at 3,661,251 to 3,662,417
_3271
Pf1N1B4_3272 - hypothetical protein, at 3,662,437 to 3,662,553
_3272
Pf1N1B4_3273 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 3,662,633 to 3,662,893
_3273
Pf1N1B4_3274 - Fosfomycin resistance protein FosA, at 3,663,201 to 3,663,617
_3274
Position (kb)
3662
3663 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3661.527 kb on + strand, within Pf1N1B4_3271 at 3661.527 kb on + strand, within Pf1N1B4_3271 at 3661.527 kb on + strand, within Pf1N1B4_3271 at 3661.528 kb on - strand, within Pf1N1B4_3271 at 3661.536 kb on - strand, within Pf1N1B4_3271 at 3661.536 kb on - strand, within Pf1N1B4_3271 at 3661.603 kb on - strand, within Pf1N1B4_3271 at 3661.660 kb on + strand, within Pf1N1B4_3271 at 3661.661 kb on - strand, within Pf1N1B4_3271 at 3661.661 kb on - strand, within Pf1N1B4_3271 at 3661.828 kb on + strand, within Pf1N1B4_3271 at 3661.931 kb on - strand, within Pf1N1B4_3271 at 3661.931 kb on - strand, within Pf1N1B4_3271 at 3661.931 kb on - strand, within Pf1N1B4_3271 at 3661.931 kb on - strand, within Pf1N1B4_3271 at 3661.931 kb on - strand, within Pf1N1B4_3271 at 3661.979 kb on - strand, within Pf1N1B4_3271 at 3662.179 kb on + strand, within Pf1N1B4_3271 at 3662.179 kb on + strand, within Pf1N1B4_3271 at 3662.179 kb on + strand, within Pf1N1B4_3271 at 3662.180 kb on - strand, within Pf1N1B4_3271 at 3662.180 kb on - strand, within Pf1N1B4_3271 at 3662.180 kb on - strand at 3662.180 kb on - strand, within Pf1N1B4_3271 at 3662.180 kb on - strand, within Pf1N1B4_3271 at 3662.184 kb on - strand, within Pf1N1B4_3271 at 3662.272 kb on + strand, within Pf1N1B4_3271 at 3662.272 kb on + strand, within Pf1N1B4_3271 at 3662.272 kb on + strand, within Pf1N1B4_3271 at 3662.273 kb on - strand, within Pf1N1B4_3271 at 3662.273 kb on - strand, within Pf1N1B4_3271 at 3662.273 kb on - strand, within Pf1N1B4_3271 at 3662.314 kb on - strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.460 kb on - strand, within Pf1N1B4_3272 at 3662.499 kb on + strand, within Pf1N1B4_3272 at 3662.707 kb on + strand, within Pf1N1B4_3273 at 3662.707 kb on + strand, within Pf1N1B4_3273 at 3662.708 kb on - strand, within Pf1N1B4_3273 at 3662.708 kb on - strand, within Pf1N1B4_3273 at 3662.708 kb on - strand, within Pf1N1B4_3273 at 3663.139 kb on + strand at 3663.139 kb on + strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.156 kb on - strand at 3663.156 kb on - strand at 3663.162 kb on + strand at 3663.162 kb on + strand at 3663.163 kb on - strand at 3663.163 kb on - strand at 3663.163 kb on - strand at 3663.184 kb on + strand at 3663.184 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.191 kb on + strand at 3663.289 kb on + strand, within Pf1N1B4_3274 at 3663.289 kb on + strand, within Pf1N1B4_3274 at 3663.290 kb on - strand, within Pf1N1B4_3274 at 3663.290 kb on - strand, within Pf1N1B4_3274 at 3663.290 kb on - strand, within Pf1N1B4_3274 at 3663.315 kb on + strand, within Pf1N1B4_3274 at 3663.316 kb on - strand, within Pf1N1B4_3274 at 3663.387 kb on + strand, within Pf1N1B4_3274 at 3663.387 kb on + strand, within Pf1N1B4_3274 at 3663.388 kb on - strand, within Pf1N1B4_3274 at 3663.502 kb on - strand, within Pf1N1B4_3274 at 3663.502 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 3,661,527 + Pf1N1B4_3271 0.24 +1.8 3,661,527 + Pf1N1B4_3271 0.24 +0.1 3,661,527 + Pf1N1B4_3271 0.24 +0.4 3,661,528 - Pf1N1B4_3271 0.24 +1.2 3,661,536 - Pf1N1B4_3271 0.24 +1.9 3,661,536 - Pf1N1B4_3271 0.24 +0.9 3,661,603 - Pf1N1B4_3271 0.30 -2.8 3,661,660 + Pf1N1B4_3271 0.35 +0.4 3,661,661 - Pf1N1B4_3271 0.35 -0.9 3,661,661 - Pf1N1B4_3271 0.35 -0.1 3,661,828 + Pf1N1B4_3271 0.49 -0.1 3,661,931 - Pf1N1B4_3271 0.58 +0.7 3,661,931 - Pf1N1B4_3271 0.58 +0.9 3,661,931 - Pf1N1B4_3271 0.58 -0.9 3,661,931 - Pf1N1B4_3271 0.58 -1.2 3,661,931 - Pf1N1B4_3271 0.58 +0.5 3,661,979 - Pf1N1B4_3271 0.62 -1.4 3,662,179 + Pf1N1B4_3271 0.80 +0.7 3,662,179 + Pf1N1B4_3271 0.80 -0.1 3,662,179 + Pf1N1B4_3271 0.80 -0.1 3,662,180 - Pf1N1B4_3271 0.80 -0.2 3,662,180 - Pf1N1B4_3271 0.80 -0.4 3,662,180 - +1.5 3,662,180 - Pf1N1B4_3271 0.80 +1.3 3,662,180 - Pf1N1B4_3271 0.80 -0.6 3,662,184 - Pf1N1B4_3271 0.80 +0.4 3,662,272 + Pf1N1B4_3271 0.87 -0.5 3,662,272 + Pf1N1B4_3271 0.87 -0.5 3,662,272 + Pf1N1B4_3271 0.87 -0.1 3,662,273 - Pf1N1B4_3271 0.88 +0.4 3,662,273 - Pf1N1B4_3271 0.88 -1.8 3,662,273 - Pf1N1B4_3271 0.88 -0.8 3,662,314 - -1.4 3,662,347 + +1.5 3,662,347 + +1.5 3,662,347 + +0.4 3,662,347 + -0.9 3,662,347 + -0.1 3,662,347 + +1.9 3,662,348 - -0.2 3,662,348 - +0.9 3,662,348 - +0.9 3,662,348 - -1.4 3,662,348 - +1.5 3,662,348 - +0.1 3,662,348 - +0.4 3,662,348 - -3.4 3,662,348 - +0.4 3,662,460 - Pf1N1B4_3272 0.20 -2.2 3,662,499 + Pf1N1B4_3272 0.53 -0.8 3,662,707 + Pf1N1B4_3273 0.28 -0.1 3,662,707 + Pf1N1B4_3273 0.28 +0.4 3,662,708 - Pf1N1B4_3273 0.29 +0.5 3,662,708 - Pf1N1B4_3273 0.29 +1.3 3,662,708 - Pf1N1B4_3273 0.29 +0.8 3,663,139 + +0.2 3,663,139 + +0.4 3,663,140 - +0.7 3,663,140 - -0.6 3,663,140 - +0.4 3,663,140 - -2.2 3,663,140 - +0.3 3,663,156 - +0.6 3,663,156 - -0.3 3,663,162 + +0.9 3,663,162 + -0.4 3,663,163 - -0.1 3,663,163 - -0.4 3,663,163 - +0.6 3,663,184 + +0.5 3,663,184 + -0.1 3,663,186 + -1.4 3,663,186 + +0.0 3,663,186 + -0.1 3,663,186 + -0.1 3,663,186 + -0.4 3,663,187 - +0.2 3,663,187 - -0.1 3,663,187 - +0.9 3,663,187 - -0.4 3,663,187 - -1.1 3,663,187 - -0.6 3,663,191 + -0.4 3,663,289 + Pf1N1B4_3274 0.21 -0.9 3,663,289 + Pf1N1B4_3274 0.21 +0.1 3,663,290 - Pf1N1B4_3274 0.21 -0.6 3,663,290 - Pf1N1B4_3274 0.21 -1.4 3,663,290 - Pf1N1B4_3274 0.21 -0.4 3,663,315 + Pf1N1B4_3274 0.27 +0.2 3,663,316 - Pf1N1B4_3274 0.28 -3.0 3,663,387 + Pf1N1B4_3274 0.45 -0.1 3,663,387 + Pf1N1B4_3274 0.45 +0.5 3,663,388 - Pf1N1B4_3274 0.45 +0.7 3,663,502 - Pf1N1B4_3274 0.72 -0.1 3,663,502 - +1.4
Or see this region's nucleotide sequence