Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3228 and Pf1N1B4_3229 are separated by 416 nucleotides Pf1N1B4_3229 and Pf1N1B4_3230 are separated by 666 nucleotides
Pf1N1B4_3228 - Low-affinity inorganic phosphate transporter, at 3,613,770 to 3,615,206
_3228
Pf1N1B4_3229 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,615,623 to 3,615,823
_3229
Pf1N1B4_3230 - Pca regulon regulatory protein PcaR, at 3,616,490 to 3,617,332
_3230
Position (kb)
3615
3616 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3614.715 kb on - strand, within Pf1N1B4_3228 at 3615.116 kb on - strand at 3615.311 kb on + strand at 3615.312 kb on - strand at 3615.327 kb on - strand at 3615.327 kb on - strand at 3615.343 kb on + strand at 3615.387 kb on - strand at 3615.387 kb on - strand at 3615.463 kb on + strand at 3615.463 kb on + strand at 3615.464 kb on - strand at 3615.501 kb on + strand at 3615.501 kb on + strand at 3615.501 kb on + strand at 3615.540 kb on - strand at 3615.564 kb on + strand at 3615.564 kb on + strand at 3615.565 kb on - strand at 3615.577 kb on + strand at 3615.577 kb on + strand at 3615.578 kb on - strand at 3615.586 kb on + strand at 3615.586 kb on + strand at 3615.586 kb on + strand at 3615.586 kb on + strand at 3615.586 kb on + strand at 3615.586 kb on + strand at 3615.586 kb on + strand at 3615.586 kb on + strand at 3615.586 kb on + strand at 3615.586 kb on + strand at 3615.586 kb on + strand at 3615.586 kb on + strand at 3615.586 kb on + strand at 3615.587 kb on - strand at 3615.587 kb on - strand at 3615.587 kb on - strand at 3615.587 kb on - strand at 3615.587 kb on - strand at 3615.587 kb on - strand at 3615.587 kb on - strand at 3615.703 kb on + strand, within Pf1N1B4_3229 at 3615.741 kb on + strand, within Pf1N1B4_3229 at 3615.741 kb on + strand, within Pf1N1B4_3229 at 3615.741 kb on + strand, within Pf1N1B4_3229 at 3615.741 kb on + strand, within Pf1N1B4_3229 at 3615.741 kb on + strand, within Pf1N1B4_3229 at 3615.741 kb on + strand, within Pf1N1B4_3229 at 3615.741 kb on + strand, within Pf1N1B4_3229 at 3615.741 kb on + strand, within Pf1N1B4_3229 at 3615.742 kb on - strand, within Pf1N1B4_3229 at 3615.742 kb on - strand, within Pf1N1B4_3229 at 3615.742 kb on - strand, within Pf1N1B4_3229 at 3615.774 kb on + strand, within Pf1N1B4_3229 at 3615.774 kb on + strand, within Pf1N1B4_3229 at 3615.774 kb on + strand, within Pf1N1B4_3229 at 3615.774 kb on + strand, within Pf1N1B4_3229 at 3615.774 kb on + strand, within Pf1N1B4_3229 at 3615.774 kb on + strand, within Pf1N1B4_3229 at 3615.774 kb on + strand, within Pf1N1B4_3229 at 3615.774 kb on + strand, within Pf1N1B4_3229 at 3615.775 kb on - strand, within Pf1N1B4_3229 at 3615.829 kb on + strand at 3615.829 kb on + strand at 3615.829 kb on + strand at 3615.829 kb on + strand at 3615.829 kb on + strand at 3615.830 kb on - strand at 3615.848 kb on + strand at 3615.849 kb on - strand at 3615.878 kb on - strand at 3615.878 kb on - strand at 3615.878 kb on - strand at 3615.987 kb on + strand at 3615.987 kb on + strand at 3615.987 kb on + strand at 3615.987 kb on + strand at 3615.987 kb on + strand at 3615.987 kb on + strand at 3615.988 kb on - strand at 3615.988 kb on - strand at 3615.988 kb on - strand at 3616.028 kb on + strand at 3616.028 kb on + strand at 3616.028 kb on + strand at 3616.029 kb on - strand at 3616.029 kb on - strand at 3616.072 kb on + strand at 3616.072 kb on + strand at 3616.103 kb on + strand at 3616.131 kb on + strand at 3616.131 kb on + strand at 3616.131 kb on + strand at 3616.132 kb on - strand at 3616.155 kb on + strand at 3616.156 kb on - strand at 3616.156 kb on - strand at 3616.156 kb on - strand at 3616.169 kb on + strand at 3616.170 kb on - strand at 3616.175 kb on + strand at 3616.225 kb on + strand at 3616.225 kb on + strand at 3616.225 kb on + strand at 3616.225 kb on + strand at 3616.226 kb on - strand at 3616.226 kb on - strand at 3616.226 kb on - strand at 3616.226 kb on - strand at 3616.246 kb on - strand at 3616.246 kb on - strand at 3616.270 kb on + strand at 3616.270 kb on + strand at 3616.284 kb on + strand at 3616.285 kb on - strand at 3616.287 kb on + strand at 3616.288 kb on - strand at 3616.304 kb on - strand at 3616.320 kb on + strand at 3616.329 kb on + strand at 3616.329 kb on + strand at 3616.329 kb on + strand at 3616.329 kb on + strand at 3616.329 kb on + strand at 3616.330 kb on - strand at 3616.330 kb on - strand at 3616.330 kb on - strand at 3616.330 kb on - strand at 3616.361 kb on + strand at 3616.361 kb on + strand at 3616.361 kb on + strand at 3616.361 kb on + strand at 3616.362 kb on - strand at 3616.362 kb on - strand at 3616.363 kb on + strand at 3616.363 kb on + strand at 3616.363 kb on + strand at 3616.363 kb on + strand at 3616.363 kb on + strand at 3616.363 kb on + strand at 3616.363 kb on + strand at 3616.364 kb on - strand at 3616.364 kb on - strand at 3616.436 kb on - strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.456 kb on + strand at 3616.457 kb on - strand at 3616.457 kb on - strand at 3616.457 kb on - strand at 3616.457 kb on - strand at 3616.457 kb on - strand at 3616.457 kb on - strand at 3616.457 kb on - strand at 3616.457 kb on - strand at 3616.457 kb on - strand at 3616.457 kb on - strand at 3616.460 kb on - strand at 3616.460 kb on - strand at 3616.710 kb on + strand, within Pf1N1B4_3230 at 3616.710 kb on + strand, within Pf1N1B4_3230 at 3616.710 kb on + strand, within Pf1N1B4_3230 at 3616.710 kb on + strand, within Pf1N1B4_3230 at 3616.710 kb on + strand at 3616.710 kb on + strand, within Pf1N1B4_3230 at 3616.710 kb on + strand, within Pf1N1B4_3230 at 3616.710 kb on + strand, within Pf1N1B4_3230 at 3616.711 kb on - strand, within Pf1N1B4_3230 at 3616.711 kb on - strand, within Pf1N1B4_3230 at 3616.711 kb on - strand, within Pf1N1B4_3230 at 3616.711 kb on - strand, within Pf1N1B4_3230 at 3616.711 kb on - strand, within Pf1N1B4_3230 at 3616.711 kb on - strand, within Pf1N1B4_3230 at 3616.711 kb on - strand, within Pf1N1B4_3230 at 3616.711 kb on - strand, within Pf1N1B4_3230 at 3616.714 kb on + strand, within Pf1N1B4_3230 at 3616.714 kb on + strand, within Pf1N1B4_3230 at 3616.714 kb on + strand, within Pf1N1B4_3230 at 3616.714 kb on + strand, within Pf1N1B4_3230 at 3616.715 kb on - strand, within Pf1N1B4_3230 at 3616.715 kb on - strand, within Pf1N1B4_3230 at 3616.757 kb on + strand, within Pf1N1B4_3230 at 3616.757 kb on + strand, within Pf1N1B4_3230 at 3616.757 kb on + strand, within Pf1N1B4_3230 at 3616.757 kb on + strand, within Pf1N1B4_3230 at 3616.757 kb on + strand, within Pf1N1B4_3230 at 3616.758 kb on - strand, within Pf1N1B4_3230 at 3616.758 kb on - strand, within Pf1N1B4_3230 at 3616.758 kb on - strand, within Pf1N1B4_3230 at 3616.758 kb on - strand, within Pf1N1B4_3230 at 3616.758 kb on - strand, within Pf1N1B4_3230 at 3616.776 kb on + strand, within Pf1N1B4_3230 at 3616.776 kb on + strand, within Pf1N1B4_3230 at 3616.776 kb on + strand, within Pf1N1B4_3230 at 3616.776 kb on + strand, within Pf1N1B4_3230 at 3616.776 kb on + strand, within Pf1N1B4_3230 at 3616.776 kb on + strand, within Pf1N1B4_3230 at 3616.777 kb on - strand, within Pf1N1B4_3230 at 3616.777 kb on - strand, within Pf1N1B4_3230 at 3616.777 kb on - strand, within Pf1N1B4_3230 at 3616.777 kb on - strand, within Pf1N1B4_3230 at 3616.777 kb on - strand, within Pf1N1B4_3230 at 3616.796 kb on + strand, within Pf1N1B4_3230 at 3616.796 kb on + strand, within Pf1N1B4_3230 at 3616.796 kb on + strand, within Pf1N1B4_3230 at 3616.796 kb on + strand, within Pf1N1B4_3230 at 3616.796 kb on + strand, within Pf1N1B4_3230 at 3616.796 kb on + strand, within Pf1N1B4_3230 at 3616.796 kb on + strand, within Pf1N1B4_3230 at 3616.796 kb on + strand, within Pf1N1B4_3230 at 3616.796 kb on + strand, within Pf1N1B4_3230 at 3616.796 kb on + strand, within Pf1N1B4_3230 at 3616.796 kb on + strand at 3616.797 kb on - strand, within Pf1N1B4_3230 at 3616.797 kb on - strand, within Pf1N1B4_3230 at 3616.797 kb on - strand, within Pf1N1B4_3230
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 3,614,715 - Pf1N1B4_3228 0.66 -0.3 3,615,116 - -0.0 3,615,311 + -0.6 3,615,312 - -1.0 3,615,327 - +1.6 3,615,327 - +0.5 3,615,343 + +3.0 3,615,387 - +0.4 3,615,387 - -1.9 3,615,463 + -0.4 3,615,463 + +0.2 3,615,464 - -0.0 3,615,501 + +0.6 3,615,501 + -0.3 3,615,501 + +2.0 3,615,540 - -0.0 3,615,564 + +2.1 3,615,564 + +0.7 3,615,565 - -0.8 3,615,577 + +0.6 3,615,577 + +0.2 3,615,578 - -0.9 3,615,586 + -0.3 3,615,586 + -0.0 3,615,586 + -0.8 3,615,586 + +0.5 3,615,586 + +0.4 3,615,586 + +0.9 3,615,586 + +0.1 3,615,586 + +0.8 3,615,586 + -0.2 3,615,586 + -0.0 3,615,586 + +1.7 3,615,586 + -0.1 3,615,586 + -0.6 3,615,587 - +1.0 3,615,587 - +0.5 3,615,587 - +1.7 3,615,587 - +0.4 3,615,587 - -1.3 3,615,587 - +0.2 3,615,587 - +1.3 3,615,703 + Pf1N1B4_3229 0.40 +1.0 3,615,741 + Pf1N1B4_3229 0.59 +0.8 3,615,741 + Pf1N1B4_3229 0.59 +1.0 3,615,741 + Pf1N1B4_3229 0.59 +1.3 3,615,741 + Pf1N1B4_3229 0.59 -1.0 3,615,741 + Pf1N1B4_3229 0.59 +0.3 3,615,741 + Pf1N1B4_3229 0.59 -0.8 3,615,741 + Pf1N1B4_3229 0.59 +1.6 3,615,741 + Pf1N1B4_3229 0.59 +0.4 3,615,742 - Pf1N1B4_3229 0.59 +0.4 3,615,742 - Pf1N1B4_3229 0.59 +0.6 3,615,742 - Pf1N1B4_3229 0.59 -1.6 3,615,774 + Pf1N1B4_3229 0.75 +0.3 3,615,774 + Pf1N1B4_3229 0.75 +1.1 3,615,774 + Pf1N1B4_3229 0.75 +0.8 3,615,774 + Pf1N1B4_3229 0.75 +0.7 3,615,774 + Pf1N1B4_3229 0.75 +0.5 3,615,774 + Pf1N1B4_3229 0.75 +1.0 3,615,774 + Pf1N1B4_3229 0.75 +1.2 3,615,774 + Pf1N1B4_3229 0.75 +0.3 3,615,775 - Pf1N1B4_3229 0.76 +0.4 3,615,829 + +0.3 3,615,829 + -1.1 3,615,829 + -1.6 3,615,829 + +0.8 3,615,829 + +0.9 3,615,830 - -0.3 3,615,848 + +0.2 3,615,849 - +1.0 3,615,878 - +0.8 3,615,878 - +0.2 3,615,878 - +1.0 3,615,987 + -0.3 3,615,987 + -0.0 3,615,987 + +0.6 3,615,987 + +1.0 3,615,987 + -2.6 3,615,987 + -0.1 3,615,988 - -1.1 3,615,988 - -0.5 3,615,988 - -0.0 3,616,028 + +0.1 3,616,028 + +1.6 3,616,028 + -0.5 3,616,029 - +0.5 3,616,029 - +2.0 3,616,072 + +1.7 3,616,072 + -0.8 3,616,103 + +0.2 3,616,131 + -0.5 3,616,131 + -0.5 3,616,131 + -0.3 3,616,132 - -0.3 3,616,155 + -0.4 3,616,156 - -1.0 3,616,156 - -0.9 3,616,156 - -0.0 3,616,169 + -1.2 3,616,170 - +0.7 3,616,175 + +0.4 3,616,225 + -0.0 3,616,225 + +0.5 3,616,225 + -0.6 3,616,225 + +0.6 3,616,226 - -0.9 3,616,226 - +1.0 3,616,226 - -0.6 3,616,226 - +0.7 3,616,246 - +1.0 3,616,246 - +1.0 3,616,270 + -2.7 3,616,270 + -1.2 3,616,284 + -0.3 3,616,285 - -0.3 3,616,287 + -0.8 3,616,288 - +1.2 3,616,304 - -0.8 3,616,320 + +1.2 3,616,329 + +1.2 3,616,329 + +1.0 3,616,329 + +0.5 3,616,329 + -1.8 3,616,329 + +0.7 3,616,330 - -1.3 3,616,330 - -0.4 3,616,330 - +1.2 3,616,330 - -0.0 3,616,361 + -1.6 3,616,361 + +1.1 3,616,361 + +1.0 3,616,361 + +0.4 3,616,362 - +0.6 3,616,362 - +0.4 3,616,363 + -0.2 3,616,363 + +1.3 3,616,363 + -0.8 3,616,363 + -0.2 3,616,363 + -1.6 3,616,363 + -1.3 3,616,363 + -0.1 3,616,364 - -1.8 3,616,364 - +2.0 3,616,436 - -1.2 3,616,456 + -0.2 3,616,456 + +1.0 3,616,456 + -1.3 3,616,456 + -2.3 3,616,456 + -0.8 3,616,456 + -0.2 3,616,456 + +0.2 3,616,456 + -0.0 3,616,456 + +0.6 3,616,456 + -0.4 3,616,456 + +0.3 3,616,456 + -0.6 3,616,456 + -2.0 3,616,456 + -1.0 3,616,457 - -0.2 3,616,457 - -0.3 3,616,457 - -0.0 3,616,457 - +0.2 3,616,457 - -1.0 3,616,457 - -0.3 3,616,457 - -0.2 3,616,457 - -0.0 3,616,457 - -0.0 3,616,457 - -0.5 3,616,460 - +2.0 3,616,460 - +0.8 3,616,710 + Pf1N1B4_3230 0.26 +2.3 3,616,710 + Pf1N1B4_3230 0.26 +2.3 3,616,710 + Pf1N1B4_3230 0.26 +1.7 3,616,710 + Pf1N1B4_3230 0.26 +0.4 3,616,710 + +1.0 3,616,710 + Pf1N1B4_3230 0.26 -0.9 3,616,710 + Pf1N1B4_3230 0.26 -1.0 3,616,710 + Pf1N1B4_3230 0.26 +1.7 3,616,711 - Pf1N1B4_3230 0.26 +2.0 3,616,711 - Pf1N1B4_3230 0.26 +0.7 3,616,711 - Pf1N1B4_3230 0.26 -0.5 3,616,711 - Pf1N1B4_3230 0.26 -0.6 3,616,711 - Pf1N1B4_3230 0.26 +0.7 3,616,711 - Pf1N1B4_3230 0.26 +0.8 3,616,711 - Pf1N1B4_3230 0.26 -1.5 3,616,711 - Pf1N1B4_3230 0.26 -0.6 3,616,714 + Pf1N1B4_3230 0.27 +1.7 3,616,714 + Pf1N1B4_3230 0.27 +2.0 3,616,714 + Pf1N1B4_3230 0.27 +0.2 3,616,714 + Pf1N1B4_3230 0.27 +0.6 3,616,715 - Pf1N1B4_3230 0.27 -0.5 3,616,715 - Pf1N1B4_3230 0.27 -0.0 3,616,757 + Pf1N1B4_3230 0.32 -1.8 3,616,757 + Pf1N1B4_3230 0.32 -1.0 3,616,757 + Pf1N1B4_3230 0.32 -0.8 3,616,757 + Pf1N1B4_3230 0.32 +0.6 3,616,757 + Pf1N1B4_3230 0.32 -1.8 3,616,758 - Pf1N1B4_3230 0.32 -1.6 3,616,758 - Pf1N1B4_3230 0.32 +0.2 3,616,758 - Pf1N1B4_3230 0.32 +0.6 3,616,758 - Pf1N1B4_3230 0.32 +0.4 3,616,758 - Pf1N1B4_3230 0.32 +0.3 3,616,776 + Pf1N1B4_3230 0.34 +0.2 3,616,776 + Pf1N1B4_3230 0.34 -0.8 3,616,776 + Pf1N1B4_3230 0.34 -2.0 3,616,776 + Pf1N1B4_3230 0.34 +0.1 3,616,776 + Pf1N1B4_3230 0.34 -0.0 3,616,776 + Pf1N1B4_3230 0.34 -0.8 3,616,777 - Pf1N1B4_3230 0.34 +0.6 3,616,777 - Pf1N1B4_3230 0.34 +0.0 3,616,777 - Pf1N1B4_3230 0.34 -2.0 3,616,777 - Pf1N1B4_3230 0.34 +0.6 3,616,777 - Pf1N1B4_3230 0.34 +1.0 3,616,796 + Pf1N1B4_3230 0.36 -0.0 3,616,796 + Pf1N1B4_3230 0.36 -0.6 3,616,796 + Pf1N1B4_3230 0.36 -0.3 3,616,796 + Pf1N1B4_3230 0.36 -1.6 3,616,796 + Pf1N1B4_3230 0.36 +0.4 3,616,796 + Pf1N1B4_3230 0.36 -1.3 3,616,796 + Pf1N1B4_3230 0.36 +1.4 3,616,796 + Pf1N1B4_3230 0.36 -2.2 3,616,796 + Pf1N1B4_3230 0.36 -2.0 3,616,796 + Pf1N1B4_3230 0.36 -3.2 3,616,796 + +1.6 3,616,797 - Pf1N1B4_3230 0.36 -0.6 3,616,797 - Pf1N1B4_3230 0.36 -0.1 3,616,797 - Pf1N1B4_3230 0.36 -1.0
Or see this region's nucleotide sequence