Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3200

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	1-ethyl-3-methylimidazolium lysine 5 mM
3,582,475	+				-1.6
3,582,475	+				+1.7
3,582,476	-				-1.2
3,582,476	-				-1.3
3,582,479	+				-0.5
3,582,480	-				+0.3
3,582,480	-				-0.8
3,582,480	-				+1.5
3,582,480	-				+0.3
3,582,480	-				+1.5
3,582,658	+		Pf1N1B4_3199	0.74	-0.6
3,582,658	+		Pf1N1B4_3199	0.74	+1.3
3,582,658	+				+1.0
3,582,659	-				+1.0
3,582,659	-		Pf1N1B4_3199	0.74	-0.1
3,582,659	-		Pf1N1B4_3199	0.74	-0.6
3,582,659	-		Pf1N1B4_3199	0.74	+3.0
3,582,659	-		Pf1N1B4_3199	0.74	-0.2
3,582,659	-		Pf1N1B4_3199	0.74	+0.1
3,582,659	-		Pf1N1B4_3199	0.74	-0.8
3,582,659	-		Pf1N1B4_3199	0.74	+0.7
3,582,659	-				+1.0
3,582,676	+		Pf1N1B4_3199	0.81	-0.1
3,582,676	+		Pf1N1B4_3199	0.81	+0.6
3,582,676	+		Pf1N1B4_3199	0.81	-0.3
3,582,677	-		Pf1N1B4_3199	0.82	-1.0
3,582,677	-		Pf1N1B4_3199	0.82	-0.5
3,582,677	-		Pf1N1B4_3199	0.82	-1.1
3,582,677	-		Pf1N1B4_3199	0.82	+0.4
3,582,677	-		Pf1N1B4_3199	0.82	+0.0
3,582,712	+				-1.8
3,582,712	+				-0.0
3,582,712	+				+1.0
3,582,713	-				-0.0
3,582,713	-				+0.6
3,582,713	-				+1.5
3,582,713	-				-1.5
3,582,713	-				+1.0
3,582,713	-				+1.3
3,582,713	-				+1.0
3,582,751	+				-1.3
3,582,752	-				+1.4
3,582,752	-				-0.7
3,582,767	+				-0.2
3,582,767	+				+1.0
3,582,767	+				-0.0
3,582,767	+				+0.1
3,582,768	-				-0.7
3,582,792	+				-1.3
3,582,857	+				+1.0
3,582,858	-				-0.6
3,582,888	+				-0.3
3,582,888	+				+0.6
3,582,888	+				+0.2
3,582,888	+				-0.0
3,582,889	-				-0.0
3,582,889	-				+1.0
3,582,889	-				-1.3
3,582,894	+				+0.2
3,582,894	+				+0.3
3,582,894	+				-0.3
3,582,894	+				-0.7
3,582,894	+				-0.5
3,582,894	+				+2.0
3,582,895	-				-0.7
3,582,895	-				-0.8
3,582,976	+		Pf1N1B4_3200	0.19	-1.0
3,582,976	+		Pf1N1B4_3200	0.19	-1.0
3,582,976	+		Pf1N1B4_3200	0.19	-0.3
3,582,977	-		Pf1N1B4_3200	0.19	-0.6
3,582,977	-				+0.4
3,583,261	+		Pf1N1B4_3200	0.80	-0.6
3,583,261	+		Pf1N1B4_3200	0.80	-0.0
3,583,261	+		Pf1N1B4_3200	0.80	-0.4
3,583,351	+				-1.0
3,583,367	+				-0.0
3,583,422	+				-0.6
3,583,423	-				-0.0
3,583,423	-				-1.3
3,583,423	-				+3.0
3,583,423	-				-0.8
3,583,423	-				-0.6
3,583,428	-				+0.1
3,583,428	-				-1.8
3,583,471	+				-0.3
3,583,481	-				-0.4
3,583,481	-				-0.8
3,583,481	-				-1.5
3,583,481	-				-0.6
3,583,542	+				-0.0
3,583,543	-				+0.5
3,583,543	-				+1.0
3,583,543	-				-0.2
3,583,569	-				+0.1
3,583,569	-				-0.3
3,583,611	+				+0.7
3,583,612	-				-2.5
3,583,612	-				+2.0
3,583,612	-				+0.7
3,583,628	+				+0.4
3,583,629	-				+1.0
3,583,647	+				-1.8
3,583,648	-				-1.0
3,583,648	-				-0.8
3,583,691	-				-0.6
3,583,691	-				+0.3
3,583,725	+				+1.0
3,583,726	-				+0.6
3,583,726	-				-0.8
3,583,740	+				-0.6
3,583,740	+				-1.0
3,583,741	-				-0.3
3,583,758	+				-0.2
3,583,758	+				-1.0
3,583,759	-				-0.8
3,583,759	-				-0.8
3,583,759	-				+2.3
3,583,819	+		Pf1N1B4_3201	0.11	-1.0
3,583,820	-		Pf1N1B4_3201	0.11	-0.2
3,583,820	-		Pf1N1B4_3201	0.11	-0.7
3,583,949	-				+2.0
3,583,997	+		Pf1N1B4_3201	0.26	-1.8
3,583,997	+		Pf1N1B4_3201	0.26	-0.2
3,583,997	+		Pf1N1B4_3201	0.26	-1.3
3,583,997	+		Pf1N1B4_3201	0.26	-1.3
3,583,997	+		Pf1N1B4_3201	0.26	+0.7
3,583,997	+				-0.6
3,583,997	+		Pf1N1B4_3201	0.26	+0.4
3,583,997	+		Pf1N1B4_3201	0.26	-0.6
3,583,997	+		Pf1N1B4_3201	0.26	-0.6
3,583,998	-		Pf1N1B4_3201	0.26	+0.6
3,583,998	-		Pf1N1B4_3201	0.26	-1.3
3,583,998	-		Pf1N1B4_3201	0.26	-0.8
3,583,998	-		Pf1N1B4_3201	0.26	-0.3
3,583,998	-		Pf1N1B4_3201	0.26	+0.3
3,583,998	-		Pf1N1B4_3201	0.26	+0.1
3,583,998	-		Pf1N1B4_3201	0.26	-1.3
3,583,998	-		Pf1N1B4_3201	0.26	-0.6
3,583,998	-		Pf1N1B4_3201	0.26	-0.3
3,583,998	-		Pf1N1B4_3201	0.26	-1.8
3,583,998	-		Pf1N1B4_3201	0.26	-0.7
3,583,998	-		Pf1N1B4_3201	0.26	-1.3
3,583,998	-		Pf1N1B4_3201	0.26	+1.0
3,583,998	-		Pf1N1B4_3201	0.26	-1.2
3,583,999	+		Pf1N1B4_3201	0.26	+1.0
3,583,999	+		Pf1N1B4_3201	0.26	-0.0
3,583,999	+		Pf1N1B4_3201	0.26	-0.0
3,583,999	+		Pf1N1B4_3201	0.26	-2.6
3,583,999	+		Pf1N1B4_3201	0.26	+0.7
3,583,999	+		Pf1N1B4_3201	0.26	-0.6
3,584,000	-		Pf1N1B4_3201	0.26	-2.8
3,584,000	-		Pf1N1B4_3201	0.26	-0.0
3,584,000	-		Pf1N1B4_3201	0.26	-0.3
3,584,000	-		Pf1N1B4_3201	0.26	-1.6
3,584,000	-		Pf1N1B4_3201	0.26	-1.9
3,584,000	-		Pf1N1B4_3201	0.26	-1.3
3,584,050	+		Pf1N1B4_3201	0.30	+0.3
3,584,050	+		Pf1N1B4_3201	0.30	+1.0
3,584,050	+		Pf1N1B4_3201	0.30	-2.0
3,584,050	+		Pf1N1B4_3201	0.30	-1.3
3,584,051	-		Pf1N1B4_3201	0.30	-0.4
3,584,062	+		Pf1N1B4_3201	0.31	+1.3
3,584,063	-		Pf1N1B4_3201	0.31	+0.7
3,584,199	+		Pf1N1B4_3201	0.42	-0.2
3,584,199	+		Pf1N1B4_3201	0.42	-0.1
3,584,199	+		Pf1N1B4_3201	0.42	-1.6
3,584,200	-		Pf1N1B4_3201	0.42	-0.7
3,584,200	-		Pf1N1B4_3201	0.42	+0.7
3,584,200	-				+3.3
3,584,200	-		Pf1N1B4_3201	0.42	+0.1
3,584,200	-		Pf1N1B4_3201	0.42	-0.0
3,584,200	-		Pf1N1B4_3201	0.42	-0.4
3,584,202	-		Pf1N1B4_3201	0.42	+1.0
3,584,202	-		Pf1N1B4_3201	0.42	-1.3
3,584,202	-		Pf1N1B4_3201	0.42	-0.0
3,584,291	-		Pf1N1B4_3201	0.50	-0.4
3,584,343	+		Pf1N1B4_3201	0.54	+1.7
3,584,344	-		Pf1N1B4_3201	0.54	+0.6