Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3194 and Pf1N1B4_3195 are separated by 96 nucleotides Pf1N1B4_3195 and Pf1N1B4_3196 are separated by 227 nucleotides
Pf1N1B4_3194 - ATP-dependent helicase DinG/Rad3, at 3,574,459 to 3,576,603
_3194
Pf1N1B4_3195 - hydrolase, putative, at 3,576,700 to 3,579,222
_3195
Pf1N1B4_3196 - Esterase A, at 3,579,450 to 3,580,595
_3196
Position (kb)
3576
3577
3578
3579
3580 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3575.887 kb on + strand, within Pf1N1B4_3194 at 3576.620 kb on + strand at 3576.620 kb on + strand at 3576.620 kb on + strand at 3576.620 kb on + strand at 3576.620 kb on + strand at 3576.621 kb on - strand at 3576.621 kb on - strand at 3576.638 kb on + strand at 3576.638 kb on + strand at 3576.639 kb on - strand at 3576.639 kb on - strand at 3576.639 kb on - strand at 3576.639 kb on - strand at 3576.700 kb on - strand at 3577.016 kb on - strand, within Pf1N1B4_3195 at 3577.113 kb on + strand, within Pf1N1B4_3195 at 3577.113 kb on + strand, within Pf1N1B4_3195 at 3577.114 kb on - strand, within Pf1N1B4_3195 at 3577.178 kb on + strand at 3577.178 kb on + strand, within Pf1N1B4_3195 at 3577.342 kb on + strand, within Pf1N1B4_3195 at 3577.579 kb on + strand, within Pf1N1B4_3195 at 3577.580 kb on - strand, within Pf1N1B4_3195 at 3577.580 kb on - strand, within Pf1N1B4_3195 at 3577.624 kb on + strand at 3577.624 kb on + strand, within Pf1N1B4_3195 at 3577.624 kb on + strand, within Pf1N1B4_3195 at 3577.625 kb on - strand, within Pf1N1B4_3195 at 3577.625 kb on - strand, within Pf1N1B4_3195 at 3577.687 kb on + strand, within Pf1N1B4_3195 at 3577.687 kb on + strand at 3577.688 kb on - strand, within Pf1N1B4_3195 at 3577.688 kb on - strand at 3577.691 kb on - strand, within Pf1N1B4_3195 at 3577.741 kb on + strand, within Pf1N1B4_3195 at 3578.129 kb on - strand, within Pf1N1B4_3195 at 3578.129 kb on - strand, within Pf1N1B4_3195 at 3578.159 kb on - strand, within Pf1N1B4_3195 at 3578.363 kb on - strand, within Pf1N1B4_3195 at 3578.561 kb on - strand, within Pf1N1B4_3195 at 3578.587 kb on + strand, within Pf1N1B4_3195 at 3578.587 kb on + strand, within Pf1N1B4_3195 at 3578.588 kb on - strand, within Pf1N1B4_3195 at 3578.718 kb on + strand, within Pf1N1B4_3195 at 3578.718 kb on + strand, within Pf1N1B4_3195 at 3578.718 kb on + strand, within Pf1N1B4_3195 at 3578.719 kb on - strand, within Pf1N1B4_3195 at 3578.719 kb on - strand, within Pf1N1B4_3195 at 3578.719 kb on - strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.723 kb on - strand, within Pf1N1B4_3195 at 3578.723 kb on - strand, within Pf1N1B4_3195 at 3578.723 kb on - strand, within Pf1N1B4_3195 at 3578.941 kb on + strand, within Pf1N1B4_3195 at 3579.220 kb on + strand at 3579.221 kb on - strand at 3579.290 kb on + strand at 3579.348 kb on + strand at 3579.349 kb on - strand at 3579.421 kb on + strand at 3579.421 kb on + strand at 3579.421 kb on + strand at 3579.422 kb on - strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.424 kb on - strand at 3579.424 kb on - strand at 3579.424 kb on - strand at 3579.468 kb on + strand at 3579.468 kb on + strand at 3579.469 kb on - strand at 3579.469 kb on - strand at 3579.476 kb on + strand at 3579.476 kb on + strand at 3579.476 kb on + strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.844 kb on - strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.952 kb on + strand, within Pf1N1B4_3196 at 3579.953 kb on - strand, within Pf1N1B4_3196 at 3579.953 kb on - strand, within Pf1N1B4_3196 at 3580.181 kb on + strand, within Pf1N1B4_3196 at 3580.181 kb on + strand, within Pf1N1B4_3196 at 3580.181 kb on + strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.183 kb on + strand, within Pf1N1B4_3196 at 3580.183 kb on + strand, within Pf1N1B4_3196 at 3580.190 kb on + strand, within Pf1N1B4_3196 at 3580.190 kb on + strand, within Pf1N1B4_3196 at 3580.191 kb on - strand, within Pf1N1B4_3196
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 3,575,887 + Pf1N1B4_3194 0.67 -2.5 3,576,620 + -0.3 3,576,620 + -0.0 3,576,620 + -0.6 3,576,620 + +1.2 3,576,620 + +1.0 3,576,621 - +1.0 3,576,621 - +0.2 3,576,638 + +0.1 3,576,638 + +0.5 3,576,639 - -1.3 3,576,639 - +0.4 3,576,639 - -1.8 3,576,639 - +1.0 3,576,700 - -1.0 3,577,016 - Pf1N1B4_3195 0.13 -1.1 3,577,113 + Pf1N1B4_3195 0.16 -0.0 3,577,113 + Pf1N1B4_3195 0.16 -0.0 3,577,114 - Pf1N1B4_3195 0.16 +0.7 3,577,178 + -0.0 3,577,178 + Pf1N1B4_3195 0.19 -1.9 3,577,342 + Pf1N1B4_3195 0.25 +0.4 3,577,579 + Pf1N1B4_3195 0.35 -0.0 3,577,580 - Pf1N1B4_3195 0.35 -0.0 3,577,580 - Pf1N1B4_3195 0.35 -0.0 3,577,624 + -1.3 3,577,624 + Pf1N1B4_3195 0.37 -0.2 3,577,624 + Pf1N1B4_3195 0.37 -1.0 3,577,625 - Pf1N1B4_3195 0.37 -0.0 3,577,625 - Pf1N1B4_3195 0.37 -1.0 3,577,687 + Pf1N1B4_3195 0.39 +0.3 3,577,687 + +1.0 3,577,688 - Pf1N1B4_3195 0.39 -1.8 3,577,688 - +1.0 3,577,691 - Pf1N1B4_3195 0.39 -0.6 3,577,741 + Pf1N1B4_3195 0.41 -0.6 3,578,129 - Pf1N1B4_3195 0.57 -0.6 3,578,129 - Pf1N1B4_3195 0.57 -0.7 3,578,159 - Pf1N1B4_3195 0.58 -1.0 3,578,363 - Pf1N1B4_3195 0.66 -0.0 3,578,561 - Pf1N1B4_3195 0.74 -0.6 3,578,587 + Pf1N1B4_3195 0.75 -1.2 3,578,587 + Pf1N1B4_3195 0.75 -0.6 3,578,588 - Pf1N1B4_3195 0.75 +0.6 3,578,718 + Pf1N1B4_3195 0.80 -1.8 3,578,718 + Pf1N1B4_3195 0.80 -1.0 3,578,718 + Pf1N1B4_3195 0.80 -1.6 3,578,719 - Pf1N1B4_3195 0.80 +0.6 3,578,719 - Pf1N1B4_3195 0.80 +0.4 3,578,719 - Pf1N1B4_3195 0.80 -1.2 3,578,722 + Pf1N1B4_3195 0.80 -1.3 3,578,722 + Pf1N1B4_3195 0.80 +1.0 3,578,722 + Pf1N1B4_3195 0.80 -2.2 3,578,722 + Pf1N1B4_3195 0.80 -1.3 3,578,722 + Pf1N1B4_3195 0.80 -1.0 3,578,723 - Pf1N1B4_3195 0.80 +0.7 3,578,723 - Pf1N1B4_3195 0.80 -1.2 3,578,723 - Pf1N1B4_3195 0.80 -0.0 3,578,941 + Pf1N1B4_3195 0.89 -0.6 3,579,220 + -1.0 3,579,221 - +0.7 3,579,290 + -0.1 3,579,348 + -0.0 3,579,349 - +1.0 3,579,421 + -2.2 3,579,421 + -0.2 3,579,421 + -0.0 3,579,422 - -1.0 3,579,423 + -2.6 3,579,423 + -1.0 3,579,423 + -0.9 3,579,423 + -0.0 3,579,423 + -1.2 3,579,423 + +1.7 3,579,423 + +1.0 3,579,423 + -2.2 3,579,424 - -1.0 3,579,424 - -1.1 3,579,424 - +0.6 3,579,468 + +0.3 3,579,468 + +0.4 3,579,469 - -2.3 3,579,469 - -1.7 3,579,476 + -0.0 3,579,476 + +0.5 3,579,476 + -0.8 3,579,477 - -1.3 3,579,477 - +0.4 3,579,477 - +0.3 3,579,477 - -0.3 3,579,477 - +1.6 3,579,477 - +0.4 3,579,844 - Pf1N1B4_3196 0.34 +2.6 3,579,930 + Pf1N1B4_3196 0.42 +1.0 3,579,930 + Pf1N1B4_3196 0.42 +1.4 3,579,930 + Pf1N1B4_3196 0.42 +0.6 3,579,930 + Pf1N1B4_3196 0.42 +2.6 3,579,930 + Pf1N1B4_3196 0.42 -0.1 3,579,930 + Pf1N1B4_3196 0.42 -0.0 3,579,931 - Pf1N1B4_3196 0.42 -0.0 3,579,931 - Pf1N1B4_3196 0.42 +1.6 3,579,931 - Pf1N1B4_3196 0.42 -0.6 3,579,931 - Pf1N1B4_3196 0.42 -1.3 3,579,931 - Pf1N1B4_3196 0.42 -0.1 3,579,931 - Pf1N1B4_3196 0.42 -0.7 3,579,952 + Pf1N1B4_3196 0.44 +1.0 3,579,953 - Pf1N1B4_3196 0.44 +0.4 3,579,953 - Pf1N1B4_3196 0.44 -1.0 3,580,181 + Pf1N1B4_3196 0.64 +0.5 3,580,181 + Pf1N1B4_3196 0.64 +0.3 3,580,181 + Pf1N1B4_3196 0.64 +1.8 3,580,182 - Pf1N1B4_3196 0.64 +0.6 3,580,182 - Pf1N1B4_3196 0.64 +0.4 3,580,182 - Pf1N1B4_3196 0.64 +0.1 3,580,182 - Pf1N1B4_3196 0.64 -2.0 3,580,183 + Pf1N1B4_3196 0.64 -1.9 3,580,183 + Pf1N1B4_3196 0.64 -1.3 3,580,190 + Pf1N1B4_3196 0.65 +1.0 3,580,190 + Pf1N1B4_3196 0.65 -0.0 3,580,191 - Pf1N1B4_3196 0.65 -0.0
Or see this region's nucleotide sequence