Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2866

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2864 and Pf1N1B4_2865 overlap by 4 nucleotidesPf1N1B4_2865 and Pf1N1B4_2866 are separated by 163 nucleotidesPf1N1B4_2866 and Pf1N1B4_2867 overlap by 4 nucleotides Pf1N1B4_2864 - Sugar/maltose fermentation stimulation protein homolog, at 3,212,584 to 3,213,297 _2864 Pf1N1B4_2865 - Rieske 2Fe-2S family protein, at 3,213,294 to 3,213,611 _2865 Pf1N1B4_2866 - Periplasmic hemin-binding protein, at 3,213,775 to 3,214,647 _2866 Pf1N1B4_2867 - Hemin ABC transporter, permease protein, at 3,214,644 to 3,215,681 _2867 Position (kb) 3213 3214 3215Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3212.920 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strandat 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.977 kb on + strandat 3212.977 kb on + strand, within Pf1N1B4_2864at 3212.978 kb on - strand, within Pf1N1B4_2864at 3212.978 kb on - strand, within Pf1N1B4_2864at 3212.978 kb on - strand, within Pf1N1B4_2864at 3212.978 kb on - strand, within Pf1N1B4_2864at 3212.978 kb on - strand, within Pf1N1B4_2864at 3212.978 kb on - strand, within Pf1N1B4_2864at 3212.978 kb on - strand, within Pf1N1B4_2864at 3212.978 kb on - strand, within Pf1N1B4_2864at 3213.118 kb on + strand, within Pf1N1B4_2864at 3213.118 kb on + strand, within Pf1N1B4_2864at 3213.118 kb on + strand, within Pf1N1B4_2864at 3213.119 kb on - strand, within Pf1N1B4_2864at 3213.119 kb on - strandat 3213.119 kb on - strand, within Pf1N1B4_2864at 3213.119 kb on - strand, within Pf1N1B4_2864at 3213.119 kb on - strand, within Pf1N1B4_2864at 3213.119 kb on - strand, within Pf1N1B4_2864at 3213.119 kb on - strand, within Pf1N1B4_2864at 3213.229 kb on + strandat 3213.229 kb on + strandat 3213.230 kb on - strandat 3213.287 kb on + strandat 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strand, within Pf1N1B4_2865at 3213.508 kb on + strandat 3213.509 kb on - strand, within Pf1N1B4_2865at 3213.509 kb on - strand, within Pf1N1B4_2865at 3213.509 kb on - strand, within Pf1N1B4_2865at 3213.509 kb on - strand, within Pf1N1B4_2865at 3213.509 kb on - strand, within Pf1N1B4_2865at 3213.509 kb on - strand, within Pf1N1B4_2865at 3213.509 kb on - strand, within Pf1N1B4_2865at 3213.509 kb on - strand, within Pf1N1B4_2865at 3213.509 kb on - strand, within Pf1N1B4_2865at 3213.509 kb on - strand, within Pf1N1B4_2865at 3213.509 kb on - strand, within Pf1N1B4_2865at 3213.509 kb on - strand, within Pf1N1B4_2865at 3213.616 kb on - strandat 3213.645 kb on + strandat 3213.645 kb on + strandat 3213.645 kb on + strandat 3213.645 kb on + strandat 3213.645 kb on + strandat 3213.645 kb on + strandat 3213.645 kb on + strandat 3213.645 kb on + strandat 3213.645 kb on + strandat 3213.645 kb on + strandat 3213.645 kb on + strandat 3213.646 kb on - strandat 3213.646 kb on - strandat 3213.646 kb on - strandat 3213.646 kb on - strandat 3213.646 kb on - strandat 3213.646 kb on - strandat 3213.646 kb on - strandat 3213.646 kb on - strandat 3213.678 kb on + strandat 3213.679 kb on - strandat 3213.724 kb on + strandat 3213.724 kb on + strandat 3213.725 kb on - strandat 3213.725 kb on - strandat 3213.761 kb on - strandat 3213.774 kb on + strandat 3213.775 kb on - strandat 3213.775 kb on - strandat 3214.207 kb on + strand, within Pf1N1B4_2866at 3214.207 kb on + strand, within Pf1N1B4_2866at 3214.208 kb on - strand, within Pf1N1B4_2866at 3214.208 kb on - strandat 3214.277 kb on + strand, within Pf1N1B4_2866at 3214.390 kb on + strand, within Pf1N1B4_2866at 3214.406 kb on + strand, within Pf1N1B4_2866at 3214.406 kb on + strandat 3214.442 kb on + strand, within Pf1N1B4_2866at 3214.442 kb on + strand, within Pf1N1B4_2866at 3214.442 kb on + strand, within Pf1N1B4_2866at 3214.442 kb on + strand, within Pf1N1B4_2866at 3214.443 kb on - strand, within Pf1N1B4_2866at 3214.625 kb on - strandat 3214.754 kb on + strand, within Pf1N1B4_2867at 3214.912 kb on + strand, within Pf1N1B4_2867at 3214.912 kb on + strand, within Pf1N1B4_2867at 3214.912 kb on + strand, within Pf1N1B4_2867at 3214.913 kb on - strand, within Pf1N1B4_2867at 3215.074 kb on + strand, within Pf1N1B4_2867at 3215.074 kb on + strand, within Pf1N1B4_2867at 3215.088 kb on + strand, within Pf1N1B4_2867at 3215.190 kb on + strand, within Pf1N1B4_2867at 3215.190 kb on + strand, within Pf1N1B4_2867at 3215.190 kb on + strand, within Pf1N1B4_2867at 3215.259 kb on + strand, within Pf1N1B4_2867at 3215.260 kb on - strand, within Pf1N1B4_2867at 3215.284 kb on + strand, within Pf1N1B4_2867

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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3,212,920 + Pf1N1B4_2864 0.47 +0.9
3,212,977 + Pf1N1B4_2864 0.55 -1.1
3,212,977 + Pf1N1B4_2864 0.55 +1.5
3,212,977 + Pf1N1B4_2864 0.55 -1.4
3,212,977 + Pf1N1B4_2864 0.55 +0.5
3,212,977 + Pf1N1B4_2864 0.55 -1.6
3,212,977 + Pf1N1B4_2864 0.55 -1.0
3,212,977 + +0.2
3,212,977 + Pf1N1B4_2864 0.55 -0.0
3,212,977 + Pf1N1B4_2864 0.55 +0.2
3,212,977 + Pf1N1B4_2864 0.55 +0.4
3,212,977 + Pf1N1B4_2864 0.55 +0.0
3,212,977 + Pf1N1B4_2864 0.55 +1.2
3,212,977 + Pf1N1B4_2864 0.55 -0.9
3,212,977 + +0.2
3,212,977 + Pf1N1B4_2864 0.55 -0.8
3,212,978 - Pf1N1B4_2864 0.55 -0.6
3,212,978 - Pf1N1B4_2864 0.55 +0.9
3,212,978 - Pf1N1B4_2864 0.55 +0.2
3,212,978 - Pf1N1B4_2864 0.55 +0.8
3,212,978 - Pf1N1B4_2864 0.55 +0.6
3,212,978 - Pf1N1B4_2864 0.55 +1.1
3,212,978 - Pf1N1B4_2864 0.55 +1.0
3,212,978 - Pf1N1B4_2864 0.55 -0.4
3,213,118 + Pf1N1B4_2864 0.75 -0.6
3,213,118 + Pf1N1B4_2864 0.75 +0.6
3,213,118 + Pf1N1B4_2864 0.75 -0.2
3,213,119 - Pf1N1B4_2864 0.75 +0.1
3,213,119 - -0.8
3,213,119 - Pf1N1B4_2864 0.75 +0.8
3,213,119 - Pf1N1B4_2864 0.75 +0.2
3,213,119 - Pf1N1B4_2864 0.75 -0.8
3,213,119 - Pf1N1B4_2864 0.75 +0.2
3,213,119 - Pf1N1B4_2864 0.75 -0.7
3,213,229 + +1.2
3,213,229 + -0.4
3,213,230 - +0.5
3,213,287 + -1.0
3,213,508 + Pf1N1B4_2865 0.67 +0.9
3,213,508 + Pf1N1B4_2865 0.67 +0.6
3,213,508 + Pf1N1B4_2865 0.67 +0.3
3,213,508 + Pf1N1B4_2865 0.67 +1.5
3,213,508 + Pf1N1B4_2865 0.67 +1.2
3,213,508 + Pf1N1B4_2865 0.67 -0.8
3,213,508 + Pf1N1B4_2865 0.67 -0.8
3,213,508 + Pf1N1B4_2865 0.67 +0.2
3,213,508 + Pf1N1B4_2865 0.67 +2.0
3,213,508 + Pf1N1B4_2865 0.67 -0.6
3,213,508 + Pf1N1B4_2865 0.67 +1.2
3,213,508 + Pf1N1B4_2865 0.67 +0.2
3,213,508 + Pf1N1B4_2865 0.67 -2.0
3,213,508 + +3.2
3,213,509 - Pf1N1B4_2865 0.68 -0.2
3,213,509 - Pf1N1B4_2865 0.68 +1.2
3,213,509 - Pf1N1B4_2865 0.68 +0.5
3,213,509 - Pf1N1B4_2865 0.68 -0.0
3,213,509 - Pf1N1B4_2865 0.68 +1.2
3,213,509 - Pf1N1B4_2865 0.68 -0.4
3,213,509 - Pf1N1B4_2865 0.68 +0.2
3,213,509 - Pf1N1B4_2865 0.68 -0.8
3,213,509 - Pf1N1B4_2865 0.68 -1.1
3,213,509 - Pf1N1B4_2865 0.68 +0.8
3,213,509 - Pf1N1B4_2865 0.68 +0.5
3,213,509 - Pf1N1B4_2865 0.68 +0.2
3,213,616 - -0.8
3,213,645 + -0.8
3,213,645 + +0.8
3,213,645 + -1.1
3,213,645 + -0.6
3,213,645 + -1.9
3,213,645 + -0.8
3,213,645 + +0.2
3,213,645 + +0.7
3,213,645 + -2.0
3,213,645 + +0.2
3,213,645 + +3.2
3,213,646 - +0.9
3,213,646 - +0.2
3,213,646 - +0.6
3,213,646 - -0.4
3,213,646 - +0.6
3,213,646 - +0.4
3,213,646 - -0.4
3,213,646 - -0.0
3,213,678 + -0.0
3,213,679 - +0.5
3,213,724 + -1.2
3,213,724 + +1.8
3,213,725 - +1.4
3,213,725 - -0.2
3,213,761 - -1.7
3,213,774 + -1.1
3,213,775 - -0.4
3,213,775 - +0.2
3,214,207 + Pf1N1B4_2866 0.49 -0.4
3,214,207 + Pf1N1B4_2866 0.49 -2.1
3,214,208 - Pf1N1B4_2866 0.50 -1.8
3,214,208 - +0.2
3,214,277 + Pf1N1B4_2866 0.58 +1.2
3,214,390 + Pf1N1B4_2866 0.70 -0.8
3,214,406 + Pf1N1B4_2866 0.72 +0.5
3,214,406 + +2.2
3,214,442 + Pf1N1B4_2866 0.76 -2.1
3,214,442 + Pf1N1B4_2866 0.76 -1.6
3,214,442 + Pf1N1B4_2866 0.76 -2.0
3,214,442 + Pf1N1B4_2866 0.76 -0.1
3,214,443 - Pf1N1B4_2866 0.77 +1.8
3,214,625 - -0.5
3,214,754 + Pf1N1B4_2867 0.11 -1.1
3,214,912 + Pf1N1B4_2867 0.26 +0.5
3,214,912 + Pf1N1B4_2867 0.26 -0.0
3,214,912 + Pf1N1B4_2867 0.26 -0.2
3,214,913 - Pf1N1B4_2867 0.26 +0.5
3,215,074 + Pf1N1B4_2867 0.41 -0.4
3,215,074 + Pf1N1B4_2867 0.41 +0.5
3,215,088 + Pf1N1B4_2867 0.43 -0.7
3,215,190 + Pf1N1B4_2867 0.53 -0.7
3,215,190 + Pf1N1B4_2867 0.53 -0.4
3,215,190 + Pf1N1B4_2867 0.53 +0.8
3,215,259 + Pf1N1B4_2867 0.59 -0.1
3,215,260 - Pf1N1B4_2867 0.59 -2.0
3,215,284 + Pf1N1B4_2867 0.62 -0.1

Or see this region's nucleotide sequence