Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2843 and Pf1N1B4_2844 are separated by 317 nucleotides Pf1N1B4_2844 and Pf1N1B4_2845 are separated by 204 nucleotides Pf1N1B4_2845 and Pf1N1B4_2846 overlap by 4 nucleotides
Pf1N1B4_2843 - Type IV pilin PilA, at 3,188,418 to 3,188,840
_2843
Pf1N1B4_2844 - Osmotically inducible protein OsmY, at 3,189,158 to 3,189,514
_2844
Pf1N1B4_2845 - FIG00953066: hypothetical protein, at 3,189,719 to 3,190,018
_2845
Pf1N1B4_2846 - putative exported protein, at 3,190,015 to 3,192,960
_2846
Position (kb)
3189
3190
3191 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3188.726 kb on - strand, within Pf1N1B4_2843 at 3188.799 kb on + strand at 3188.799 kb on + strand at 3188.799 kb on + strand at 3188.799 kb on + strand at 3188.799 kb on + strand at 3188.799 kb on + strand at 3188.799 kb on + strand at 3188.800 kb on - strand at 3188.800 kb on - strand at 3188.800 kb on - strand at 3188.807 kb on + strand at 3188.808 kb on - strand at 3188.834 kb on + strand at 3188.834 kb on + strand at 3188.983 kb on - strand at 3189.142 kb on + strand at 3189.143 kb on - strand at 3189.143 kb on - strand at 3189.151 kb on + strand at 3189.151 kb on + strand at 3189.152 kb on - strand at 3189.157 kb on + strand at 3189.158 kb on - strand at 3189.513 kb on - strand at 3189.552 kb on - strand at 3189.567 kb on + strand at 3189.567 kb on + strand at 3189.567 kb on + strand at 3189.568 kb on - strand at 3189.568 kb on - strand at 3189.568 kb on - strand at 3189.579 kb on + strand at 3189.580 kb on - strand at 3189.652 kb on + strand at 3189.652 kb on + strand at 3189.652 kb on + strand at 3189.653 kb on - strand at 3189.653 kb on - strand at 3189.663 kb on - strand at 3189.709 kb on + strand at 3189.709 kb on + strand at 3189.709 kb on - strand at 3189.710 kb on - strand at 3189.710 kb on - strand at 3189.724 kb on + strand at 3189.725 kb on - strand at 3189.725 kb on - strand at 3189.725 kb on - strand at 3189.775 kb on - strand, within Pf1N1B4_2845 at 3189.780 kb on + strand, within Pf1N1B4_2845 at 3189.780 kb on + strand, within Pf1N1B4_2845 at 3189.780 kb on + strand, within Pf1N1B4_2845 at 3189.780 kb on + strand, within Pf1N1B4_2845 at 3189.780 kb on + strand, within Pf1N1B4_2845 at 3189.780 kb on + strand at 3189.781 kb on - strand, within Pf1N1B4_2845 at 3189.781 kb on - strand, within Pf1N1B4_2845 at 3189.802 kb on - strand, within Pf1N1B4_2845 at 3189.802 kb on - strand, within Pf1N1B4_2845 at 3189.802 kb on - strand, within Pf1N1B4_2845 at 3189.802 kb on - strand, within Pf1N1B4_2845 at 3189.853 kb on - strand, within Pf1N1B4_2845 at 3189.949 kb on + strand, within Pf1N1B4_2845 at 3189.949 kb on + strand, within Pf1N1B4_2845 at 3189.950 kb on - strand, within Pf1N1B4_2845 at 3189.950 kb on - strand, within Pf1N1B4_2845 at 3189.950 kb on - strand, within Pf1N1B4_2845 at 3190.091 kb on + strand at 3190.091 kb on + strand at 3190.092 kb on - strand at 3190.092 kb on - strand at 3190.130 kb on + strand at 3190.130 kb on + strand at 3190.131 kb on - strand at 3190.131 kb on - strand at 3190.131 kb on - strand at 3190.131 kb on - strand at 3190.131 kb on - strand at 3190.289 kb on + strand at 3190.289 kb on + strand at 3190.289 kb on + strand at 3190.290 kb on - strand at 3190.355 kb on - strand, within Pf1N1B4_2846 at 3190.385 kb on + strand, within Pf1N1B4_2846 at 3190.385 kb on + strand, within Pf1N1B4_2846 at 3190.385 kb on + strand, within Pf1N1B4_2846 at 3190.386 kb on - strand, within Pf1N1B4_2846 at 3190.386 kb on - strand, within Pf1N1B4_2846 at 3190.388 kb on + strand, within Pf1N1B4_2846 at 3190.388 kb on + strand, within Pf1N1B4_2846 at 3190.388 kb on + strand, within Pf1N1B4_2846 at 3190.388 kb on + strand, within Pf1N1B4_2846 at 3190.388 kb on + strand, within Pf1N1B4_2846 at 3190.389 kb on - strand, within Pf1N1B4_2846 at 3190.389 kb on - strand, within Pf1N1B4_2846 at 3190.389 kb on - strand, within Pf1N1B4_2846 at 3190.389 kb on - strand, within Pf1N1B4_2846 at 3190.389 kb on - strand, within Pf1N1B4_2846 at 3190.389 kb on - strand, within Pf1N1B4_2846 at 3190.394 kb on + strand, within Pf1N1B4_2846 at 3190.394 kb on + strand, within Pf1N1B4_2846 at 3190.394 kb on + strand, within Pf1N1B4_2846 at 3190.394 kb on + strand, within Pf1N1B4_2846 at 3190.394 kb on + strand, within Pf1N1B4_2846 at 3190.394 kb on + strand, within Pf1N1B4_2846 at 3190.395 kb on - strand, within Pf1N1B4_2846 at 3190.400 kb on + strand, within Pf1N1B4_2846 at 3190.401 kb on - strand, within Pf1N1B4_2846 at 3190.401 kb on - strand, within Pf1N1B4_2846 at 3190.671 kb on + strand, within Pf1N1B4_2846 at 3190.671 kb on + strand, within Pf1N1B4_2846 at 3190.671 kb on + strand, within Pf1N1B4_2846 at 3190.671 kb on + strand, within Pf1N1B4_2846 at 3190.672 kb on - strand, within Pf1N1B4_2846 at 3190.672 kb on - strand, within Pf1N1B4_2846 at 3190.672 kb on - strand, within Pf1N1B4_2846 at 3190.672 kb on - strand, within Pf1N1B4_2846 at 3190.672 kb on - strand, within Pf1N1B4_2846 at 3190.674 kb on + strand, within Pf1N1B4_2846 at 3190.674 kb on + strand, within Pf1N1B4_2846 at 3190.674 kb on + strand, within Pf1N1B4_2846 at 3190.674 kb on + strand, within Pf1N1B4_2846 at 3190.674 kb on + strand, within Pf1N1B4_2846 at 3190.675 kb on - strand, within Pf1N1B4_2846 at 3190.675 kb on - strand, within Pf1N1B4_2846 at 3190.675 kb on - strand, within Pf1N1B4_2846 at 3190.675 kb on - strand, within Pf1N1B4_2846 at 3190.675 kb on - strand, within Pf1N1B4_2846 at 3190.877 kb on + strand, within Pf1N1B4_2846 at 3190.877 kb on + strand, within Pf1N1B4_2846 at 3190.877 kb on + strand, within Pf1N1B4_2846 at 3190.878 kb on - strand, within Pf1N1B4_2846 at 3190.878 kb on - strand, within Pf1N1B4_2846 at 3190.878 kb on - strand, within Pf1N1B4_2846 at 3190.937 kb on + strand, within Pf1N1B4_2846 at 3190.937 kb on + strand, within Pf1N1B4_2846 at 3190.938 kb on - strand, within Pf1N1B4_2846
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 3,188,726 - Pf1N1B4_2843 0.73 +1.0 3,188,799 + +0.8 3,188,799 + -2.1 3,188,799 + +0.2 3,188,799 + +0.7 3,188,799 + -0.5 3,188,799 + +0.2 3,188,799 + +0.4 3,188,800 - +2.2 3,188,800 - -1.6 3,188,800 - +1.2 3,188,807 + -0.7 3,188,808 - -0.8 3,188,834 + -0.9 3,188,834 + -0.2 3,188,983 - +2.2 3,189,142 + -0.2 3,189,143 - -0.8 3,189,143 - +0.1 3,189,151 + +0.1 3,189,151 + +0.2 3,189,152 - -0.0 3,189,157 + -0.2 3,189,158 - -1.8 3,189,513 - +1.6 3,189,552 - +0.4 3,189,567 + +0.8 3,189,567 + +0.9 3,189,567 + +0.5 3,189,568 - -0.4 3,189,568 - -0.4 3,189,568 - -0.6 3,189,579 + -1.0 3,189,580 - +1.5 3,189,652 + +0.2 3,189,652 + -0.2 3,189,652 + -1.8 3,189,653 - -1.1 3,189,653 - +0.5 3,189,663 - +1.4 3,189,709 + +2.2 3,189,709 + -1.2 3,189,709 - -0.4 3,189,710 - -0.5 3,189,710 - -0.4 3,189,724 + +0.6 3,189,725 - -0.5 3,189,725 - -0.1 3,189,725 - -0.8 3,189,775 - Pf1N1B4_2845 0.19 -1.0 3,189,780 + Pf1N1B4_2845 0.20 -0.1 3,189,780 + Pf1N1B4_2845 0.20 -0.7 3,189,780 + Pf1N1B4_2845 0.20 +1.6 3,189,780 + Pf1N1B4_2845 0.20 -2.2 3,189,780 + Pf1N1B4_2845 0.20 -1.1 3,189,780 + +1.2 3,189,781 - Pf1N1B4_2845 0.21 -1.8 3,189,781 - Pf1N1B4_2845 0.21 -1.6 3,189,802 - Pf1N1B4_2845 0.28 -0.4 3,189,802 - Pf1N1B4_2845 0.28 -1.1 3,189,802 - Pf1N1B4_2845 0.28 -1.4 3,189,802 - Pf1N1B4_2845 0.28 -0.4 3,189,853 - Pf1N1B4_2845 0.45 -0.4 3,189,949 + Pf1N1B4_2845 0.77 -0.2 3,189,949 + Pf1N1B4_2845 0.77 -0.2 3,189,950 - Pf1N1B4_2845 0.77 -0.6 3,189,950 - Pf1N1B4_2845 0.77 -0.8 3,189,950 - Pf1N1B4_2845 0.77 -1.0 3,190,091 + +0.8 3,190,091 + -1.0 3,190,092 - -0.2 3,190,092 - -1.6 3,190,130 + +0.1 3,190,130 + +2.8 3,190,131 - -0.4 3,190,131 - -0.2 3,190,131 - -0.4 3,190,131 - -0.6 3,190,131 - +0.8 3,190,289 + +0.1 3,190,289 + +0.2 3,190,289 + -0.8 3,190,290 - -0.2 3,190,355 - Pf1N1B4_2846 0.12 -0.6 3,190,385 + Pf1N1B4_2846 0.13 -0.2 3,190,385 + Pf1N1B4_2846 0.13 -0.4 3,190,385 + Pf1N1B4_2846 0.13 -1.6 3,190,386 - Pf1N1B4_2846 0.13 -0.8 3,190,386 - Pf1N1B4_2846 0.13 -0.4 3,190,388 + Pf1N1B4_2846 0.13 -1.4 3,190,388 + Pf1N1B4_2846 0.13 -2.1 3,190,388 + Pf1N1B4_2846 0.13 +1.2 3,190,388 + Pf1N1B4_2846 0.13 -0.1 3,190,388 + Pf1N1B4_2846 0.13 +0.2 3,190,389 - Pf1N1B4_2846 0.13 -0.6 3,190,389 - Pf1N1B4_2846 0.13 +0.1 3,190,389 - Pf1N1B4_2846 0.13 -0.5 3,190,389 - Pf1N1B4_2846 0.13 -1.4 3,190,389 - Pf1N1B4_2846 0.13 +0.6 3,190,389 - Pf1N1B4_2846 0.13 -0.3 3,190,394 + Pf1N1B4_2846 0.13 +1.2 3,190,394 + Pf1N1B4_2846 0.13 +0.2 3,190,394 + Pf1N1B4_2846 0.13 -0.7 3,190,394 + Pf1N1B4_2846 0.13 -0.6 3,190,394 + Pf1N1B4_2846 0.13 +0.2 3,190,394 + Pf1N1B4_2846 0.13 +1.0 3,190,395 - Pf1N1B4_2846 0.13 -1.0 3,190,400 + Pf1N1B4_2846 0.13 -1.0 3,190,401 - Pf1N1B4_2846 0.13 -0.1 3,190,401 - Pf1N1B4_2846 0.13 -1.2 3,190,671 + Pf1N1B4_2846 0.22 +0.9 3,190,671 + Pf1N1B4_2846 0.22 +0.5 3,190,671 + Pf1N1B4_2846 0.22 -0.8 3,190,671 + Pf1N1B4_2846 0.22 -1.8 3,190,672 - Pf1N1B4_2846 0.22 -0.1 3,190,672 - Pf1N1B4_2846 0.22 -2.6 3,190,672 - Pf1N1B4_2846 0.22 -1.2 3,190,672 - Pf1N1B4_2846 0.22 +0.4 3,190,672 - Pf1N1B4_2846 0.22 +0.6 3,190,674 + Pf1N1B4_2846 0.22 -1.6 3,190,674 + Pf1N1B4_2846 0.22 +1.2 3,190,674 + Pf1N1B4_2846 0.22 +2.2 3,190,674 + Pf1N1B4_2846 0.22 +0.5 3,190,674 + Pf1N1B4_2846 0.22 +0.2 3,190,675 - Pf1N1B4_2846 0.22 -0.8 3,190,675 - Pf1N1B4_2846 0.22 -1.4 3,190,675 - Pf1N1B4_2846 0.22 -1.6 3,190,675 - Pf1N1B4_2846 0.22 +1.8 3,190,675 - Pf1N1B4_2846 0.22 -0.8 3,190,877 + Pf1N1B4_2846 0.29 -0.5 3,190,877 + Pf1N1B4_2846 0.29 +2.2 3,190,877 + Pf1N1B4_2846 0.29 +1.5 3,190,878 - Pf1N1B4_2846 0.29 +1.9 3,190,878 - Pf1N1B4_2846 0.29 +0.8 3,190,878 - Pf1N1B4_2846 0.29 +0.8 3,190,937 + Pf1N1B4_2846 0.31 -0.1 3,190,937 + Pf1N1B4_2846 0.31 +1.8 3,190,938 - Pf1N1B4_2846 0.31 -2.2
Or see this region's nucleotide sequence