Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2532 and Pf1N1B4_2533 overlap by 4 nucleotides Pf1N1B4_2533 and Pf1N1B4_2534 are separated by 67 nucleotides Pf1N1B4_2534 and Pf1N1B4_2535 are separated by 139 nucleotides
Pf1N1B4_2532 - COG3178: Predicted phosphotransferase related to Ser/Thr protein kinases, at 2,872,850 to 2,873,689
_2532
Pf1N1B4_2533 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 2,873,686 to 2,874,357
_2533
Pf1N1B4_2534 - DnaJ-like protein DjlA, at 2,874,425 to 2,875,126
_2534
Pf1N1B4_2535 - FIG00953890: hypothetical protein, at 2,875,266 to 2,876,258
_2535
Position (kb)
2874
2875
2876 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2873.872 kb on + strand, within Pf1N1B4_2533 at 2874.329 kb on + strand at 2874.329 kb on + strand at 2874.329 kb on + strand at 2874.329 kb on + strand at 2874.329 kb on + strand at 2874.330 kb on - strand at 2874.341 kb on + strand at 2874.342 kb on - strand at 2874.490 kb on - strand at 2874.490 kb on - strand at 2874.549 kb on + strand, within Pf1N1B4_2534 at 2874.549 kb on + strand, within Pf1N1B4_2534 at 2874.721 kb on + strand, within Pf1N1B4_2534 at 2874.918 kb on - strand, within Pf1N1B4_2534 at 2875.091 kb on + strand at 2875.091 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.093 kb on + strand at 2875.094 kb on - strand at 2875.094 kb on - strand at 2875.094 kb on - strand at 2875.094 kb on - strand at 2875.217 kb on + strand at 2875.218 kb on - strand at 2875.218 kb on - strand at 2875.425 kb on - strand, within Pf1N1B4_2535 at 2875.445 kb on + strand, within Pf1N1B4_2535 at 2875.446 kb on - strand, within Pf1N1B4_2535 at 2875.447 kb on + strand, within Pf1N1B4_2535 at 2875.447 kb on + strand, within Pf1N1B4_2535 at 2875.447 kb on + strand, within Pf1N1B4_2535 at 2875.447 kb on + strand, within Pf1N1B4_2535 at 2875.447 kb on + strand, within Pf1N1B4_2535 at 2875.447 kb on + strand, within Pf1N1B4_2535 at 2875.447 kb on + strand, within Pf1N1B4_2535 at 2875.447 kb on + strand, within Pf1N1B4_2535 at 2875.447 kb on + strand, within Pf1N1B4_2535 at 2875.447 kb on + strand, within Pf1N1B4_2535 at 2875.447 kb on + strand, within Pf1N1B4_2535 at 2875.447 kb on + strand, within Pf1N1B4_2535 at 2875.448 kb on - strand, within Pf1N1B4_2535 at 2875.448 kb on - strand, within Pf1N1B4_2535 at 2875.448 kb on - strand, within Pf1N1B4_2535 at 2875.448 kb on - strand, within Pf1N1B4_2535 at 2875.448 kb on - strand, within Pf1N1B4_2535 at 2875.448 kb on - strand, within Pf1N1B4_2535 at 2875.448 kb on - strand, within Pf1N1B4_2535 at 2875.448 kb on - strand, within Pf1N1B4_2535 at 2875.448 kb on - strand, within Pf1N1B4_2535 at 2875.511 kb on + strand, within Pf1N1B4_2535 at 2875.512 kb on - strand, within Pf1N1B4_2535 at 2875.576 kb on + strand, within Pf1N1B4_2535 at 2875.577 kb on - strand, within Pf1N1B4_2535 at 2875.622 kb on + strand, within Pf1N1B4_2535 at 2875.623 kb on - strand, within Pf1N1B4_2535 at 2875.623 kb on - strand, within Pf1N1B4_2535 at 2875.623 kb on - strand, within Pf1N1B4_2535 at 2875.838 kb on + strand, within Pf1N1B4_2535 at 2875.838 kb on + strand at 2875.973 kb on + strand, within Pf1N1B4_2535 at 2875.973 kb on + strand, within Pf1N1B4_2535 at 2875.974 kb on - strand, within Pf1N1B4_2535 at 2876.106 kb on - strand, within Pf1N1B4_2535 at 2876.106 kb on - strand, within Pf1N1B4_2535
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,873,872 + Pf1N1B4_2533 0.28 -1.0 2,874,329 + +0.5 2,874,329 + -0.0 2,874,329 + -2.0 2,874,329 + +0.7 2,874,329 + -0.3 2,874,330 - +0.3 2,874,341 + +0.3 2,874,342 - -0.9 2,874,490 - +0.3 2,874,490 - -0.3 2,874,549 + Pf1N1B4_2534 0.18 -1.5 2,874,549 + Pf1N1B4_2534 0.18 -0.8 2,874,721 + Pf1N1B4_2534 0.42 -1.4 2,874,918 - Pf1N1B4_2534 0.70 +1.6 2,875,091 + -0.1 2,875,091 + +0.3 2,875,093 + -1.7 2,875,093 + -0.7 2,875,093 + -0.7 2,875,093 + +0.1 2,875,093 + -0.6 2,875,093 + -1.0 2,875,093 + -1.2 2,875,093 + +0.3 2,875,093 + -0.3 2,875,093 + -1.7 2,875,093 + +0.4 2,875,093 + +0.5 2,875,093 + -1.5 2,875,093 + +0.6 2,875,093 + +0.7 2,875,093 + -0.1 2,875,093 + +1.3 2,875,093 + -0.0 2,875,093 + -0.5 2,875,094 - -1.1 2,875,094 - -0.2 2,875,094 - -0.3 2,875,094 - +1.3 2,875,217 + +1.3 2,875,218 - -0.3 2,875,218 - -1.0 2,875,425 - Pf1N1B4_2535 0.16 -1.4 2,875,445 + Pf1N1B4_2535 0.18 +0.4 2,875,446 - Pf1N1B4_2535 0.18 -1.3 2,875,447 + Pf1N1B4_2535 0.18 +0.3 2,875,447 + Pf1N1B4_2535 0.18 +0.4 2,875,447 + Pf1N1B4_2535 0.18 -0.7 2,875,447 + Pf1N1B4_2535 0.18 -2.0 2,875,447 + Pf1N1B4_2535 0.18 -0.3 2,875,447 + Pf1N1B4_2535 0.18 -0.4 2,875,447 + Pf1N1B4_2535 0.18 +1.8 2,875,447 + Pf1N1B4_2535 0.18 -1.0 2,875,447 + Pf1N1B4_2535 0.18 -0.7 2,875,447 + Pf1N1B4_2535 0.18 +0.7 2,875,447 + Pf1N1B4_2535 0.18 +1.9 2,875,447 + Pf1N1B4_2535 0.18 -0.9 2,875,448 - Pf1N1B4_2535 0.18 -0.4 2,875,448 - Pf1N1B4_2535 0.18 +1.3 2,875,448 - Pf1N1B4_2535 0.18 -0.7 2,875,448 - Pf1N1B4_2535 0.18 +0.5 2,875,448 - Pf1N1B4_2535 0.18 -0.3 2,875,448 - Pf1N1B4_2535 0.18 +0.2 2,875,448 - Pf1N1B4_2535 0.18 -1.5 2,875,448 - Pf1N1B4_2535 0.18 -0.7 2,875,448 - Pf1N1B4_2535 0.18 -0.2 2,875,511 + Pf1N1B4_2535 0.25 -0.4 2,875,512 - Pf1N1B4_2535 0.25 +1.3 2,875,576 + Pf1N1B4_2535 0.31 -2.2 2,875,577 - Pf1N1B4_2535 0.31 -1.7 2,875,622 + Pf1N1B4_2535 0.36 -1.9 2,875,623 - Pf1N1B4_2535 0.36 -1.5 2,875,623 - Pf1N1B4_2535 0.36 -1.5 2,875,623 - Pf1N1B4_2535 0.36 -0.0 2,875,838 + Pf1N1B4_2535 0.58 +1.3 2,875,838 + +1.3 2,875,973 + Pf1N1B4_2535 0.71 +0.7 2,875,973 + Pf1N1B4_2535 0.71 +0.3 2,875,974 - Pf1N1B4_2535 0.71 +2.3 2,876,106 - Pf1N1B4_2535 0.85 +0.3 2,876,106 - Pf1N1B4_2535 0.85 -1.0
Or see this region's nucleotide sequence