Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_249 and Pf1N1B4_250 are separated by 181 nucleotides Pf1N1B4_250 and Pf1N1B4_251 are separated by 256 nucleotides Pf1N1B4_251 and Pf1N1B4_252 are separated by 8 nucleotides
Pf1N1B4_249 - Deoxyguanosinetriphosphate triphosphohydrolase (EC 3.1.5.1), at 305,561 to 306,892
_249
Pf1N1B4_250 - response regulator, at 307,074 to 307,466
_250
Pf1N1B4_251 - DNA-binding response regulator, LuxR family, at 307,723 to 308,349
_251
Pf1N1B4_252 - Hybrid sensory histidine kinase in two-component regulatory system with EvgA, at 308,358 to 311,261
_252
Position (kb) 307
308
309 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 306.745 kb on - strand, within Pf1N1B4_249 at 306.789 kb on + strand at 306.790 kb on - strand at 306.790 kb on - strand at 306.790 kb on - strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.956 kb on + strand at 306.957 kb on - strand at 306.982 kb on - strand at 307.030 kb on + strand at 307.031 kb on - strand at 307.073 kb on + strand at 307.073 kb on + strand at 307.074 kb on - strand at 307.074 kb on - strand at 307.074 kb on - strand at 307.291 kb on - strand, within Pf1N1B4_250 at 307.325 kb on - strand, within Pf1N1B4_250 at 307.555 kb on - strand at 307.593 kb on + strand at 307.597 kb on + strand at 307.644 kb on + strand at 307.738 kb on - strand at 307.799 kb on - strand, within Pf1N1B4_251 at 308.021 kb on - strand, within Pf1N1B4_251 at 308.024 kb on + strand, within Pf1N1B4_251 at 308.024 kb on + strand at 308.025 kb on - strand at 308.025 kb on - strand, within Pf1N1B4_251 at 308.075 kb on + strand, within Pf1N1B4_251 at 308.076 kb on - strand at 308.089 kb on + strand, within Pf1N1B4_251 at 308.266 kb on + strand, within Pf1N1B4_251 at 308.386 kb on + strand at 308.451 kb on + strand at 308.452 kb on - strand at 308.549 kb on + strand at 308.565 kb on + strand at 308.601 kb on + strand at 308.601 kb on + strand at 308.601 kb on + strand at 308.785 kb on - strand, within Pf1N1B4_252 at 308.874 kb on + strand, within Pf1N1B4_252 at 308.874 kb on + strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.880 kb on + strand, within Pf1N1B4_252 at 308.880 kb on + strand, within Pf1N1B4_252 at 308.880 kb on + strand, within Pf1N1B4_252 at 308.881 kb on - strand, within Pf1N1B4_252 at 308.881 kb on - strand, within Pf1N1B4_252 at 308.881 kb on - strand, within Pf1N1B4_252 at 308.934 kb on + strand, within Pf1N1B4_252 at 308.935 kb on - strand, within Pf1N1B4_252 at 308.944 kb on - strand, within Pf1N1B4_252 at 309.015 kb on + strand, within Pf1N1B4_252 at 309.015 kb on + strand, within Pf1N1B4_252 at 309.015 kb on + strand, within Pf1N1B4_252 at 309.016 kb on - strand, within Pf1N1B4_252 at 309.016 kb on - strand, within Pf1N1B4_252 at 309.016 kb on - strand, within Pf1N1B4_252 at 309.016 kb on - strand, within Pf1N1B4_252 at 309.016 kb on - strand, within Pf1N1B4_252 at 309.019 kb on + strand, within Pf1N1B4_252 at 309.019 kb on + strand, within Pf1N1B4_252 at 309.019 kb on + strand, within Pf1N1B4_252 at 309.020 kb on - strand, within Pf1N1B4_252 at 309.020 kb on - strand, within Pf1N1B4_252 at 309.020 kb on - strand, within Pf1N1B4_252 at 309.020 kb on - strand, within Pf1N1B4_252
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 306,745 - Pf1N1B4_249 0.89 -1.0 306,789 + +0.0 306,790 - -1.8 306,790 - -1.6 306,790 - +0.8 306,842 + +0.0 306,842 + -1.6 306,842 + -0.2 306,842 + +1.1 306,842 + +0.0 306,842 + +0.0 306,843 - +0.3 306,843 - -1.2 306,843 - +0.8 306,843 - +0.0 306,843 - -0.2 306,956 + +0.0 306,957 - +0.2 306,982 - +1.4 307,030 + +0.3 307,031 - -2.2 307,073 + +0.2 307,073 + +0.9 307,074 - +0.0 307,074 - +1.2 307,074 - -0.7 307,291 - Pf1N1B4_250 0.55 -1.1 307,325 - Pf1N1B4_250 0.64 -0.2 307,555 - +1.2 307,593 + -1.2 307,597 + +0.3 307,644 + -1.0 307,738 - -0.2 307,799 - Pf1N1B4_251 0.12 -1.8 308,021 - Pf1N1B4_251 0.48 +1.9 308,024 + Pf1N1B4_251 0.48 +1.3 308,024 + +1.3 308,025 - +1.8 308,025 - Pf1N1B4_251 0.48 +1.3 308,075 + Pf1N1B4_251 0.56 -0.2 308,076 - +0.3 308,089 + Pf1N1B4_251 0.58 -1.0 308,266 + Pf1N1B4_251 0.87 -0.1 308,386 + -1.1 308,451 + -1.1 308,452 - +1.8 308,549 + -2.1 308,565 + -0.2 308,601 + -0.8 308,601 + -0.6 308,601 + -0.5 308,785 - Pf1N1B4_252 0.15 +0.2 308,874 + Pf1N1B4_252 0.18 -0.4 308,874 + Pf1N1B4_252 0.18 -1.2 308,875 - Pf1N1B4_252 0.18 -0.6 308,875 - Pf1N1B4_252 0.18 -0.6 308,875 - Pf1N1B4_252 0.18 -0.2 308,875 - Pf1N1B4_252 0.18 -0.4 308,875 - Pf1N1B4_252 0.18 +0.2 308,875 - Pf1N1B4_252 0.18 +1.6 308,875 - Pf1N1B4_252 0.18 -0.7 308,880 + Pf1N1B4_252 0.18 -1.6 308,880 + Pf1N1B4_252 0.18 +0.8 308,880 + Pf1N1B4_252 0.18 -1.6 308,881 - Pf1N1B4_252 0.18 -0.4 308,881 - Pf1N1B4_252 0.18 +0.3 308,881 - Pf1N1B4_252 0.18 -0.5 308,934 + Pf1N1B4_252 0.20 -1.2 308,935 - Pf1N1B4_252 0.20 +0.3 308,944 - Pf1N1B4_252 0.20 +0.2 309,015 + Pf1N1B4_252 0.23 -0.6 309,015 + Pf1N1B4_252 0.23 -3.1 309,015 + Pf1N1B4_252 0.23 -0.8 309,016 - Pf1N1B4_252 0.23 -1.2 309,016 - Pf1N1B4_252 0.23 -0.1 309,016 - Pf1N1B4_252 0.23 -2.1 309,016 - Pf1N1B4_252 0.23 +0.1 309,016 - Pf1N1B4_252 0.23 +0.3 309,019 + Pf1N1B4_252 0.23 -1.1 309,019 + Pf1N1B4_252 0.23 -0.8 309,019 + Pf1N1B4_252 0.23 -1.2 309,020 - Pf1N1B4_252 0.23 -0.2 309,020 - Pf1N1B4_252 0.23 +0.8 309,020 - Pf1N1B4_252 0.23 -1.6 309,020 - Pf1N1B4_252 0.23 +0.8
Or see this region's nucleotide sequence