Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2488 and Pf1N1B4_2489 overlap by 8 nucleotides Pf1N1B4_2489 and Pf1N1B4_2490 overlap by 8 nucleotides Pf1N1B4_2490 and Pf1N1B4_2491 overlap by 4 nucleotides Pf1N1B4_2491 and Pf1N1B4_2492 are separated by 120 nucleotides
Pf1N1B4_2488 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 2,821,181 to 2,822,359
_2488
Pf1N1B4_2489 - Biotin synthesis protein BioH, at 2,822,352 to 2,823,083
_2489
Pf1N1B4_2490 - Biotin synthesis protein BioC, at 2,823,076 to 2,823,888
_2490
Pf1N1B4_2491 - Dethiobiotin synthetase (EC 6.3.3.3), at 2,823,885 to 2,824,565
_2491
Pf1N1B4_2492 - FIG00954771: hypothetical protein, at 2,824,686 to 2,824,982
_2492
Position (kb)
2823
2824 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2822.298 kb on + strand at 2822.298 kb on + strand at 2822.299 kb on - strand at 2822.299 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.814 kb on - strand, within Pf1N1B4_2489 at 2823.070 kb on + strand at 2823.070 kb on + strand at 2823.071 kb on - strand at 2823.071 kb on - strand at 2823.086 kb on + strand at 2823.086 kb on + strand at 2823.087 kb on - strand at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.164 kb on - strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.531 kb on + strand, within Pf1N1B4_2490 at 2823.532 kb on - strand, within Pf1N1B4_2490 at 2823.542 kb on + strand, within Pf1N1B4_2490 at 2823.543 kb on - strand, within Pf1N1B4_2490 at 2823.543 kb on - strand, within Pf1N1B4_2490 at 2823.543 kb on - strand, within Pf1N1B4_2490 at 2823.543 kb on - strand, within Pf1N1B4_2490 at 2823.553 kb on + strand, within Pf1N1B4_2490 at 2823.554 kb on - strand, within Pf1N1B4_2490 at 2823.554 kb on - strand, within Pf1N1B4_2490 at 2823.554 kb on - strand, within Pf1N1B4_2490 at 2823.625 kb on + strand, within Pf1N1B4_2490 at 2823.626 kb on - strand, within Pf1N1B4_2490 at 2823.626 kb on - strand, within Pf1N1B4_2490 at 2823.694 kb on + strand, within Pf1N1B4_2490 at 2823.811 kb on + strand at 2823.811 kb on + strand at 2823.812 kb on - strand at 2823.812 kb on - strand at 2823.812 kb on - strand at 2823.850 kb on + strand at 2823.850 kb on + strand at 2823.850 kb on + strand at 2823.851 kb on - strand at 2823.884 kb on + strand at 2823.885 kb on - strand at 2823.885 kb on - strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.898 kb on - strand at 2823.898 kb on - strand at 2823.898 kb on - strand at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.583 kb on + strand at 2824.583 kb on + strand at 2824.583 kb on + strand at 2824.730 kb on + strand, within Pf1N1B4_2492 at 2824.730 kb on + strand, within Pf1N1B4_2492 at 2824.880 kb on + strand, within Pf1N1B4_2492 at 2824.880 kb on + strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,822,298 + -1.0 2,822,298 + -1.3 2,822,299 - -1.9 2,822,299 - -1.7 2,822,305 - -1.8 2,822,305 - -0.4 2,822,305 - -1.9 2,822,305 - -1.3 2,822,305 - -1.5 2,822,305 - -2.1 2,822,813 + Pf1N1B4_2489 0.63 -1.0 2,822,813 + Pf1N1B4_2489 0.63 +0.3 2,822,813 + +0.3 2,822,813 + Pf1N1B4_2489 0.63 -1.6 2,822,813 + Pf1N1B4_2489 0.63 +0.3 2,822,813 + Pf1N1B4_2489 0.63 +0.6 2,822,813 + Pf1N1B4_2489 0.63 -2.0 2,822,813 + Pf1N1B4_2489 0.63 +1.0 2,822,813 + Pf1N1B4_2489 0.63 -1.0 2,822,813 + Pf1N1B4_2489 0.63 +1.3 2,822,813 + Pf1N1B4_2489 0.63 -1.5 2,822,814 - Pf1N1B4_2489 0.63 +0.3 2,823,070 + +1.3 2,823,070 + +0.3 2,823,071 - -2.1 2,823,071 - -0.3 2,823,086 + -1.1 2,823,086 + +0.2 2,823,087 - +0.3 2,823,163 + Pf1N1B4_2490 0.11 +1.9 2,823,163 + Pf1N1B4_2490 0.11 -1.1 2,823,163 + Pf1N1B4_2490 0.11 -2.3 2,823,163 + Pf1N1B4_2490 0.11 +1.3 2,823,164 - Pf1N1B4_2490 0.11 -1.0 2,823,209 + Pf1N1B4_2490 0.16 -1.7 2,823,209 + Pf1N1B4_2490 0.16 -1.0 2,823,209 + Pf1N1B4_2490 0.16 +0.1 2,823,210 - Pf1N1B4_2490 0.16 -1.9 2,823,210 - Pf1N1B4_2490 0.16 -1.5 2,823,210 - Pf1N1B4_2490 0.16 +0.6 2,823,531 + Pf1N1B4_2490 0.56 -0.7 2,823,532 - Pf1N1B4_2490 0.56 +0.1 2,823,542 + Pf1N1B4_2490 0.57 -2.1 2,823,543 - Pf1N1B4_2490 0.57 -1.5 2,823,543 - Pf1N1B4_2490 0.57 -1.8 2,823,543 - Pf1N1B4_2490 0.57 -2.1 2,823,543 - Pf1N1B4_2490 0.57 -2.1 2,823,553 + Pf1N1B4_2490 0.59 +0.5 2,823,554 - Pf1N1B4_2490 0.59 +0.6 2,823,554 - Pf1N1B4_2490 0.59 -1.5 2,823,554 - Pf1N1B4_2490 0.59 -1.4 2,823,625 + Pf1N1B4_2490 0.68 -0.7 2,823,626 - Pf1N1B4_2490 0.68 -1.3 2,823,626 - Pf1N1B4_2490 0.68 +0.1 2,823,694 + Pf1N1B4_2490 0.76 -1.0 2,823,811 + -0.1 2,823,811 + -0.7 2,823,812 - -0.7 2,823,812 - -0.3 2,823,812 - -0.3 2,823,850 + -2.0 2,823,850 + -1.0 2,823,850 + +0.4 2,823,851 - -0.7 2,823,884 + -0.2 2,823,885 - +0.0 2,823,885 - -0.7 2,823,897 + +2.3 2,823,897 + +0.1 2,823,897 + -0.1 2,823,897 + -0.9 2,823,897 + -0.7 2,823,898 - -0.1 2,823,898 - -1.0 2,823,898 - -1.5 2,824,089 + Pf1N1B4_2491 0.30 -0.4 2,824,089 + Pf1N1B4_2491 0.30 -1.0 2,824,089 + Pf1N1B4_2491 0.30 -1.7 2,824,089 + Pf1N1B4_2491 0.30 -1.3 2,824,090 - Pf1N1B4_2491 0.30 -1.2 2,824,090 - Pf1N1B4_2491 0.30 -0.3 2,824,090 - Pf1N1B4_2491 0.30 -1.4 2,824,090 - Pf1N1B4_2491 0.30 -2.0 2,824,090 - Pf1N1B4_2491 0.30 -2.3 2,824,583 + -0.7 2,824,583 + -1.7 2,824,583 + +1.0 2,824,730 + Pf1N1B4_2492 0.15 +0.7 2,824,730 + Pf1N1B4_2492 0.15 -0.3 2,824,880 + Pf1N1B4_2492 0.65 -0.7 2,824,880 + Pf1N1B4_2492 0.65 -2.0 2,824,881 - Pf1N1B4_2492 0.66 -0.7 2,824,881 - Pf1N1B4_2492 0.66 -0.7 2,824,881 - Pf1N1B4_2492 0.66 -1.4
Or see this region's nucleotide sequence