Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2487 and Pf1N1B4_2488 are separated by 186 nucleotides Pf1N1B4_2488 and Pf1N1B4_2489 overlap by 8 nucleotides Pf1N1B4_2489 and Pf1N1B4_2490 overlap by 8 nucleotides
Pf1N1B4_2487 - Biotin synthase (EC 2.8.1.6), at 2,819,939 to 2,820,994
_2487
Pf1N1B4_2488 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 2,821,181 to 2,822,359
_2488
Pf1N1B4_2489 - Biotin synthesis protein BioH, at 2,822,352 to 2,823,083
_2489
Pf1N1B4_2490 - Biotin synthesis protein BioC, at 2,823,076 to 2,823,888
_2490
Position (kb)
2821
2822
2823 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2820.290 kb on + strand, within Pf1N1B4_2487 at 2820.291 kb on - strand, within Pf1N1B4_2487 at 2820.401 kb on + strand, within Pf1N1B4_2487 at 2820.401 kb on + strand, within Pf1N1B4_2487 at 2820.402 kb on - strand at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.438 kb on - strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.464 kb on + strand, within Pf1N1B4_2487 at 2820.464 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.492 kb on - strand, within Pf1N1B4_2487 at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2821.002 kb on - strand at 2821.002 kb on - strand at 2821.002 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.233 kb on - strand at 2821.309 kb on + strand, within Pf1N1B4_2488 at 2821.309 kb on + strand, within Pf1N1B4_2488 at 2821.309 kb on + strand, within Pf1N1B4_2488 at 2821.319 kb on + strand, within Pf1N1B4_2488 at 2821.320 kb on - strand, within Pf1N1B4_2488 at 2821.499 kb on + strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.679 kb on - strand, within Pf1N1B4_2488 at 2821.964 kb on + strand, within Pf1N1B4_2488 at 2821.964 kb on + strand, within Pf1N1B4_2488 at 2821.964 kb on + strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2822.298 kb on + strand at 2822.298 kb on + strand at 2822.299 kb on - strand at 2822.299 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.814 kb on - strand, within Pf1N1B4_2489 at 2823.070 kb on + strand at 2823.070 kb on + strand at 2823.071 kb on - strand at 2823.071 kb on - strand at 2823.086 kb on + strand at 2823.086 kb on + strand at 2823.087 kb on - strand at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.164 kb on - strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,820,290 + Pf1N1B4_2487 0.33 +0.7 2,820,291 - Pf1N1B4_2487 0.33 -0.5 2,820,401 + Pf1N1B4_2487 0.44 -1.5 2,820,401 + Pf1N1B4_2487 0.44 -0.0 2,820,402 - -0.0 2,820,404 + Pf1N1B4_2487 0.44 -1.7 2,820,404 + Pf1N1B4_2487 0.44 -0.9 2,820,404 + Pf1N1B4_2487 0.44 -1.3 2,820,404 + Pf1N1B4_2487 0.44 -2.5 2,820,404 + Pf1N1B4_2487 0.44 -1.0 2,820,404 + Pf1N1B4_2487 0.44 -1.3 2,820,405 - Pf1N1B4_2487 0.44 +0.3 2,820,405 - Pf1N1B4_2487 0.44 -0.9 2,820,405 - Pf1N1B4_2487 0.44 -0.7 2,820,405 - Pf1N1B4_2487 0.44 +0.7 2,820,405 - Pf1N1B4_2487 0.44 -1.9 2,820,437 + Pf1N1B4_2487 0.47 -2.4 2,820,437 + Pf1N1B4_2487 0.47 +0.7 2,820,437 + Pf1N1B4_2487 0.47 -0.7 2,820,438 - Pf1N1B4_2487 0.47 +0.3 2,820,460 + Pf1N1B4_2487 0.49 -0.5 2,820,460 + Pf1N1B4_2487 0.49 -0.7 2,820,460 + Pf1N1B4_2487 0.49 +0.1 2,820,460 + Pf1N1B4_2487 0.49 -1.6 2,820,464 + Pf1N1B4_2487 0.50 -1.9 2,820,464 + Pf1N1B4_2487 0.50 +1.0 2,820,491 + Pf1N1B4_2487 0.52 -1.0 2,820,491 + Pf1N1B4_2487 0.52 -1.7 2,820,491 + Pf1N1B4_2487 0.52 -1.0 2,820,491 + Pf1N1B4_2487 0.52 +0.9 2,820,491 + Pf1N1B4_2487 0.52 -2.0 2,820,491 + Pf1N1B4_2487 0.52 -1.5 2,820,491 + Pf1N1B4_2487 0.52 +0.2 2,820,492 - Pf1N1B4_2487 0.52 -0.0 2,820,977 + -0.3 2,820,977 + +1.7 2,820,977 + -1.0 2,820,977 + +0.3 2,820,977 + +0.1 2,820,977 + -1.0 2,820,977 + +0.1 2,820,978 - -0.3 2,820,978 - +0.3 2,820,978 - -0.5 2,820,978 - +0.3 2,820,978 - -2.0 2,821,002 - -0.8 2,821,002 - +1.0 2,821,002 - -1.0 2,821,138 - -1.2 2,821,138 - -1.3 2,821,138 - -2.5 2,821,138 - -1.0 2,821,138 - -0.7 2,821,233 - -1.9 2,821,309 + Pf1N1B4_2488 0.11 -1.5 2,821,309 + Pf1N1B4_2488 0.11 -0.2 2,821,309 + Pf1N1B4_2488 0.11 -1.7 2,821,319 + Pf1N1B4_2488 0.12 -1.3 2,821,320 - Pf1N1B4_2488 0.12 -1.1 2,821,499 + Pf1N1B4_2488 0.27 +0.3 2,821,500 - Pf1N1B4_2488 0.27 -1.7 2,821,500 - Pf1N1B4_2488 0.27 -0.7 2,821,500 - Pf1N1B4_2488 0.27 -1.9 2,821,500 - Pf1N1B4_2488 0.27 -1.4 2,821,679 - Pf1N1B4_2488 0.42 -0.0 2,821,964 + Pf1N1B4_2488 0.66 -2.0 2,821,964 + Pf1N1B4_2488 0.66 +0.6 2,821,964 + Pf1N1B4_2488 0.66 +0.3 2,821,965 - Pf1N1B4_2488 0.66 -0.3 2,821,965 - Pf1N1B4_2488 0.66 -0.3 2,821,965 - Pf1N1B4_2488 0.66 -1.4 2,821,965 - Pf1N1B4_2488 0.66 -1.7 2,821,965 - Pf1N1B4_2488 0.66 -2.0 2,821,965 - Pf1N1B4_2488 0.66 -2.5 2,821,970 + Pf1N1B4_2488 0.67 -2.1 2,821,970 + Pf1N1B4_2488 0.67 -1.3 2,821,970 + Pf1N1B4_2488 0.67 -1.0 2,821,970 + Pf1N1B4_2488 0.67 -2.1 2,821,970 + Pf1N1B4_2488 0.67 -1.8 2,821,971 - Pf1N1B4_2488 0.67 -1.1 2,821,971 - Pf1N1B4_2488 0.67 -0.4 2,821,971 - Pf1N1B4_2488 0.67 -0.3 2,821,971 - Pf1N1B4_2488 0.67 -1.3 2,821,971 - Pf1N1B4_2488 0.67 +1.3 2,821,971 - Pf1N1B4_2488 0.67 -1.2 2,821,971 - Pf1N1B4_2488 0.67 +0.9 2,822,298 + -1.0 2,822,298 + -1.3 2,822,299 - -1.9 2,822,299 - -1.7 2,822,305 - -1.8 2,822,305 - -0.4 2,822,305 - -1.9 2,822,305 - -1.3 2,822,305 - -1.5 2,822,305 - -2.1 2,822,813 + Pf1N1B4_2489 0.63 -1.0 2,822,813 + Pf1N1B4_2489 0.63 +0.3 2,822,813 + +0.3 2,822,813 + Pf1N1B4_2489 0.63 -1.6 2,822,813 + Pf1N1B4_2489 0.63 +0.3 2,822,813 + Pf1N1B4_2489 0.63 +0.6 2,822,813 + Pf1N1B4_2489 0.63 -2.0 2,822,813 + Pf1N1B4_2489 0.63 +1.0 2,822,813 + Pf1N1B4_2489 0.63 -1.0 2,822,813 + Pf1N1B4_2489 0.63 +1.3 2,822,813 + Pf1N1B4_2489 0.63 -1.5 2,822,814 - Pf1N1B4_2489 0.63 +0.3 2,823,070 + +1.3 2,823,070 + +0.3 2,823,071 - -2.1 2,823,071 - -0.3 2,823,086 + -1.1 2,823,086 + +0.2 2,823,087 - +0.3 2,823,163 + Pf1N1B4_2490 0.11 +1.9 2,823,163 + Pf1N1B4_2490 0.11 -1.1 2,823,163 + Pf1N1B4_2490 0.11 -2.3 2,823,163 + Pf1N1B4_2490 0.11 +1.3 2,823,164 - Pf1N1B4_2490 0.11 -1.0 2,823,209 + Pf1N1B4_2490 0.16 -1.7 2,823,209 + Pf1N1B4_2490 0.16 -1.0 2,823,209 + Pf1N1B4_2490 0.16 +0.1 2,823,210 - Pf1N1B4_2490 0.16 -1.9 2,823,210 - Pf1N1B4_2490 0.16 -1.5 2,823,210 - Pf1N1B4_2490 0.16 +0.6
Or see this region's nucleotide sequence