Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2478 and Pf1N1B4_2479 are separated by 609 nucleotides Pf1N1B4_2479 and Pf1N1B4_2480 are separated by 214 nucleotides
Pf1N1B4_2478 - Malate synthase G (EC 2.3.3.9), at 2,810,387 to 2,812,564
_2478
Pf1N1B4_2479 - Glycine cleavage system transcriptional antiactivator GcvR, at 2,813,174 to 2,813,692
_2479
Pf1N1B4_2480 - Protein rarD, at 2,813,907 to 2,814,794
_2480
Position (kb)
2813
2814 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2812.323 kb on + strand, within Pf1N1B4_2478 at 2812.323 kb on + strand, within Pf1N1B4_2478 at 2812.323 kb on + strand, within Pf1N1B4_2478 at 2812.375 kb on + strand at 2812.376 kb on - strand at 2812.407 kb on - strand at 2812.635 kb on + strand at 2812.635 kb on + strand at 2812.636 kb on - strand at 2812.636 kb on - strand at 2812.652 kb on + strand at 2812.653 kb on - strand at 2812.653 kb on - strand at 2812.653 kb on - strand at 2812.658 kb on + strand at 2812.658 kb on + strand at 2812.658 kb on + strand at 2812.659 kb on - strand at 2812.659 kb on - strand at 2812.659 kb on - strand at 2812.767 kb on + strand at 2812.767 kb on + strand at 2812.768 kb on - strand at 2812.768 kb on - strand at 2812.771 kb on + strand at 2812.771 kb on + strand at 2812.772 kb on - strand at 2812.788 kb on + strand at 2812.789 kb on - strand at 2812.791 kb on + strand at 2812.792 kb on - strand at 2812.792 kb on - strand at 2812.823 kb on + strand at 2812.823 kb on + strand at 2812.823 kb on + strand at 2812.824 kb on - strand at 2812.824 kb on - strand at 2812.827 kb on + strand at 2812.827 kb on + strand at 2812.827 kb on + strand at 2812.828 kb on - strand at 2812.828 kb on - strand at 2812.925 kb on + strand at 2812.925 kb on + strand at 2812.926 kb on - strand at 2812.931 kb on + strand at 2812.931 kb on + strand at 2812.931 kb on + strand at 2812.931 kb on + strand at 2812.932 kb on - strand at 2813.017 kb on - strand at 2813.128 kb on + strand at 2813.128 kb on + strand at 2813.128 kb on + strand at 2813.128 kb on + strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.143 kb on - strand at 2813.143 kb on - strand at 2813.188 kb on - strand at 2813.339 kb on + strand, within Pf1N1B4_2479 at 2813.352 kb on + strand, within Pf1N1B4_2479 at 2813.353 kb on - strand, within Pf1N1B4_2479 at 2813.541 kb on + strand, within Pf1N1B4_2479 at 2813.541 kb on + strand, within Pf1N1B4_2479 at 2813.542 kb on - strand, within Pf1N1B4_2479 at 2813.699 kb on - strand at 2813.707 kb on + strand at 2813.709 kb on + strand at 2813.709 kb on + strand at 2813.709 kb on + strand at 2813.709 kb on + strand at 2813.710 kb on - strand at 2813.710 kb on - strand at 2813.710 kb on - strand at 2813.929 kb on - strand at 2813.948 kb on - strand at 2813.948 kb on - strand at 2813.969 kb on + strand at 2813.969 kb on + strand at 2813.969 kb on + strand at 2813.970 kb on - strand at 2814.056 kb on - strand, within Pf1N1B4_2480 at 2814.056 kb on - strand, within Pf1N1B4_2480 at 2814.056 kb on - strand, within Pf1N1B4_2480 at 2814.079 kb on + strand, within Pf1N1B4_2480 at 2814.079 kb on + strand, within Pf1N1B4_2480 at 2814.080 kb on - strand, within Pf1N1B4_2480 at 2814.305 kb on - strand at 2814.305 kb on - strand, within Pf1N1B4_2480 at 2814.305 kb on - strand, within Pf1N1B4_2480 at 2814.363 kb on + strand, within Pf1N1B4_2480 at 2814.364 kb on - strand, within Pf1N1B4_2480 at 2814.364 kb on - strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.479 kb on - strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.527 kb on - strand at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,812,323 + Pf1N1B4_2478 0.89 -0.2 2,812,323 + Pf1N1B4_2478 0.89 -0.5 2,812,323 + Pf1N1B4_2478 0.89 -0.4 2,812,375 + +0.1 2,812,376 - -2.0 2,812,407 - +0.3 2,812,635 + +0.9 2,812,635 + +0.5 2,812,636 - -1.7 2,812,636 - -0.7 2,812,652 + +1.6 2,812,653 - +0.9 2,812,653 - -0.3 2,812,653 - -0.8 2,812,658 + +0.9 2,812,658 + +1.9 2,812,658 + +0.3 2,812,659 - -1.0 2,812,659 - +0.7 2,812,659 - -1.7 2,812,767 + -0.7 2,812,767 + -0.5 2,812,768 - +0.0 2,812,768 - -1.1 2,812,771 + +0.3 2,812,771 + +0.5 2,812,772 - +0.8 2,812,788 + -0.1 2,812,789 - +0.3 2,812,791 + +0.7 2,812,792 - -0.7 2,812,792 - +0.0 2,812,823 + +0.6 2,812,823 + +1.0 2,812,823 + -1.3 2,812,824 - +0.1 2,812,824 - +0.3 2,812,827 + -2.0 2,812,827 + -1.7 2,812,827 + -0.3 2,812,828 - -1.8 2,812,828 - +1.7 2,812,925 + -0.7 2,812,925 + -0.3 2,812,926 - -1.0 2,812,931 + -1.1 2,812,931 + +1.9 2,812,931 + +0.1 2,812,931 + +0.1 2,812,932 - -0.7 2,813,017 - -0.5 2,813,128 + -1.8 2,813,128 + -1.5 2,813,128 + -0.3 2,813,128 + -0.3 2,813,129 - +1.3 2,813,129 - -0.5 2,813,129 - +0.0 2,813,129 - -0.5 2,813,129 - +0.7 2,813,129 - -3.0 2,813,129 - +0.1 2,813,129 - -1.0 2,813,130 + -1.2 2,813,130 + -0.3 2,813,130 + -1.5 2,813,130 + +0.3 2,813,130 + +0.6 2,813,130 + +0.0 2,813,130 + -0.8 2,813,130 + +0.7 2,813,130 + -0.7 2,813,131 - +0.3 2,813,131 - -0.0 2,813,131 - +0.3 2,813,131 - -0.7 2,813,131 - +0.9 2,813,131 - -2.3 2,813,131 - +1.3 2,813,131 - +0.5 2,813,143 - -1.0 2,813,143 - -0.7 2,813,188 - -0.3 2,813,339 + Pf1N1B4_2479 0.32 -1.5 2,813,352 + Pf1N1B4_2479 0.34 +1.6 2,813,353 - Pf1N1B4_2479 0.34 -1.3 2,813,541 + Pf1N1B4_2479 0.71 -2.1 2,813,541 + Pf1N1B4_2479 0.71 +0.7 2,813,542 - Pf1N1B4_2479 0.71 +0.3 2,813,699 - -1.0 2,813,707 + -0.5 2,813,709 + -1.7 2,813,709 + +0.3 2,813,709 + -1.4 2,813,709 + +1.8 2,813,710 - +0.0 2,813,710 - +0.7 2,813,710 - +0.3 2,813,929 - -1.3 2,813,948 - +0.0 2,813,948 - -1.3 2,813,969 + -0.5 2,813,969 + -1.3 2,813,969 + -0.3 2,813,970 - -1.7 2,814,056 - Pf1N1B4_2480 0.17 +0.3 2,814,056 - Pf1N1B4_2480 0.17 +1.0 2,814,056 - Pf1N1B4_2480 0.17 +0.9 2,814,079 + Pf1N1B4_2480 0.19 -1.7 2,814,079 + Pf1N1B4_2480 0.19 -0.7 2,814,080 - Pf1N1B4_2480 0.19 -0.0 2,814,305 - +0.3 2,814,305 - Pf1N1B4_2480 0.45 -0.4 2,814,305 - Pf1N1B4_2480 0.45 -2.8 2,814,363 + Pf1N1B4_2480 0.51 +1.5 2,814,364 - Pf1N1B4_2480 0.51 +0.6 2,814,364 - Pf1N1B4_2480 0.51 -0.1 2,814,475 + Pf1N1B4_2480 0.64 +0.5 2,814,475 + Pf1N1B4_2480 0.64 +0.4 2,814,475 + Pf1N1B4_2480 0.64 -0.3 2,814,475 + Pf1N1B4_2480 0.64 -0.3 2,814,475 + Pf1N1B4_2480 0.64 -0.1 2,814,476 - Pf1N1B4_2480 0.64 -0.7 2,814,476 - Pf1N1B4_2480 0.64 +1.1 2,814,476 - Pf1N1B4_2480 0.64 -0.1 2,814,476 - Pf1N1B4_2480 0.64 +0.7 2,814,476 - Pf1N1B4_2480 0.64 +1.0 2,814,476 - Pf1N1B4_2480 0.64 -1.0 2,814,476 - Pf1N1B4_2480 0.64 +0.0 2,814,479 - Pf1N1B4_2480 0.64 +1.3 2,814,526 + Pf1N1B4_2480 0.70 -1.0 2,814,526 + Pf1N1B4_2480 0.70 +0.9 2,814,526 + Pf1N1B4_2480 0.70 -1.3 2,814,526 + Pf1N1B4_2480 0.70 +0.1 2,814,526 + Pf1N1B4_2480 0.70 +0.0 2,814,526 + Pf1N1B4_2480 0.70 +1.3 2,814,526 + Pf1N1B4_2480 0.70 -1.2 2,814,526 + Pf1N1B4_2480 0.70 -1.0 2,814,526 + Pf1N1B4_2480 0.70 +0.7 2,814,526 + Pf1N1B4_2480 0.70 -0.8 2,814,527 - +0.3 2,814,527 - Pf1N1B4_2480 0.70 -0.4 2,814,527 - Pf1N1B4_2480 0.70 +0.8 2,814,527 - Pf1N1B4_2480 0.70 -1.1 2,814,527 - Pf1N1B4_2480 0.70 +0.2 2,814,527 - Pf1N1B4_2480 0.70 -0.5 2,814,527 - Pf1N1B4_2480 0.70 -1.1 2,814,527 - Pf1N1B4_2480 0.70 -0.7 2,814,527 - Pf1N1B4_2480 0.70 -1.0 2,814,527 - Pf1N1B4_2480 0.70 -0.0 2,814,527 - Pf1N1B4_2480 0.70 +1.9
Or see this region's nucleotide sequence