Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_246

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_243 and Pf1N1B4_244 overlap by 105 nucleotidesPf1N1B4_244 and Pf1N1B4_245 are separated by 219 nucleotidesPf1N1B4_245 and Pf1N1B4_246 are separated by 1 nucleotidesPf1N1B4_246 and Pf1N1B4_247 overlap by 4 nucleotidesPf1N1B4_247 and Pf1N1B4_248 are separated by 123 nucleotides Pf1N1B4_243 - Ammonium transporter, at 301,651 to 302,925 _243 Pf1N1B4_244 - hypothetical protein, at 302,821 to 302,946 _244 Pf1N1B4_245 - Probable transmembrane protein, at 303,166 to 303,480 _245 Pf1N1B4_246 - Probable transmembrane protein, at 303,482 to 303,871 _246 Pf1N1B4_247 - FIG00956101: hypothetical protein, at 303,868 to 304,206 _247 Pf1N1B4_248 - EAL-domain protein, at 304,330 to 305,493 _248 Position (kb) 303 304Strain fitness (log2 ratio) -2 -1 0 1 2at 302.588 kb on + strand, within Pf1N1B4_243at 302.656 kb on + strand, within Pf1N1B4_243at 302.656 kb on + strand, within Pf1N1B4_243at 302.656 kb on + strandat 302.656 kb on + strand, within Pf1N1B4_243at 302.657 kb on - strand, within Pf1N1B4_243at 302.657 kb on - strand, within Pf1N1B4_243at 302.657 kb on - strandat 302.658 kb on + strand, within Pf1N1B4_243at 302.658 kb on + strand, within Pf1N1B4_243at 302.658 kb on + strand, within Pf1N1B4_243at 302.659 kb on - strand, within Pf1N1B4_243at 302.659 kb on - strand, within Pf1N1B4_243at 302.666 kb on + strand, within Pf1N1B4_243at 302.666 kb on + strand, within Pf1N1B4_243at 302.667 kb on - strand, within Pf1N1B4_243at 302.820 kb on + strandat 302.820 kb on + strandat 302.820 kb on + strandat 302.821 kb on - strandat 303.008 kb on + strandat 303.009 kb on - strandat 303.079 kb on + strandat 303.080 kb on - strandat 303.104 kb on + strandat 303.105 kb on - strandat 303.128 kb on + strandat 303.128 kb on + strandat 303.129 kb on - strandat 303.160 kb on + strandat 303.397 kb on + strand, within Pf1N1B4_245at 303.397 kb on + strand, within Pf1N1B4_245at 303.482 kb on - strandat 303.482 kb on - strandat 303.716 kb on + strand, within Pf1N1B4_246at 303.752 kb on + strand, within Pf1N1B4_246at 303.752 kb on + strand, within Pf1N1B4_246at 303.752 kb on + strand, within Pf1N1B4_246at 303.752 kb on + strand, within Pf1N1B4_246at 303.752 kb on + strand, within Pf1N1B4_246at 303.752 kb on + strand, within Pf1N1B4_246at 303.752 kb on + strand, within Pf1N1B4_246at 303.752 kb on + strand, within Pf1N1B4_246at 303.752 kb on + strand, within Pf1N1B4_246at 303.752 kb on + strand, within Pf1N1B4_246at 303.753 kb on - strand, within Pf1N1B4_246at 304.204 kb on + strandat 304.247 kb on + strandat 304.315 kb on + strandat 304.315 kb on + strandat 304.316 kb on - strandat 304.318 kb on + strandat 304.318 kb on + strandat 304.319 kb on - strandat 304.319 kb on - strandat 304.319 kb on - strandat 304.574 kb on + strand, within Pf1N1B4_248at 304.575 kb on - strand, within Pf1N1B4_248

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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302,588 + Pf1N1B4_243 0.73 -1.1
302,656 + Pf1N1B4_243 0.79 -0.6
302,656 + Pf1N1B4_243 0.79 -0.0
302,656 + -0.2
302,656 + Pf1N1B4_243 0.79 +0.2
302,657 - Pf1N1B4_243 0.79 +1.0
302,657 - Pf1N1B4_243 0.79 -0.0
302,657 - -0.2
302,658 + Pf1N1B4_243 0.79 -0.2
302,658 + Pf1N1B4_243 0.79 -0.8
302,658 + Pf1N1B4_243 0.79 -1.7
302,659 - Pf1N1B4_243 0.79 -0.2
302,659 - Pf1N1B4_243 0.79 +1.8
302,666 + Pf1N1B4_243 0.80 -0.7
302,666 + Pf1N1B4_243 0.80 +0.5
302,667 - Pf1N1B4_243 0.80 -0.6
302,820 + -1.8
302,820 + +0.2
302,820 + +1.5
302,821 - -0.1
303,008 + -0.2
303,009 - -0.2
303,079 + -1.2
303,080 - +1.3
303,104 + +0.8
303,105 - +0.4
303,128 + +0.8
303,128 + -0.8
303,129 - -1.3
303,160 + +2.1
303,397 + Pf1N1B4_245 0.73 -0.2
303,397 + Pf1N1B4_245 0.73 +1.3
303,482 - +0.0
303,482 - +1.3
303,716 + Pf1N1B4_246 0.60 -1.2
303,752 + Pf1N1B4_246 0.69 +0.8
303,752 + Pf1N1B4_246 0.69 -0.6
303,752 + Pf1N1B4_246 0.69 -0.8
303,752 + Pf1N1B4_246 0.69 +1.1
303,752 + Pf1N1B4_246 0.69 -1.3
303,752 + Pf1N1B4_246 0.69 -0.6
303,752 + Pf1N1B4_246 0.69 -1.5
303,752 + Pf1N1B4_246 0.69 -1.6
303,752 + Pf1N1B4_246 0.69 -0.2
303,752 + Pf1N1B4_246 0.69 -1.2
303,753 - Pf1N1B4_246 0.69 +1.4
304,204 + -0.2
304,247 + -0.4
304,315 + +0.2
304,315 + +0.1
304,316 - -1.4
304,318 + +0.0
304,318 + -0.2
304,319 - +0.8
304,319 - -0.2
304,319 - +0.2
304,574 + Pf1N1B4_248 0.21 +0.0
304,575 - Pf1N1B4_248 0.21 +0.4

Or see this region's nucleotide sequence