Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2368 and Pf1N1B4_2369 are separated by 1 nucleotides Pf1N1B4_2369 and Pf1N1B4_2370 are separated by 106 nucleotides Pf1N1B4_2370 and Pf1N1B4_2371 are separated by 241 nucleotides Pf1N1B4_2371 and Pf1N1B4_2372 are separated by 58 nucleotides
Pf1N1B4_2368 - Malonate transporter, MadL subunit, at 2,684,618 to 2,685,034
_2368
Pf1N1B4_2369 - Malonate transporter, MadM subunit, at 2,685,036 to 2,685,800
_2369
Pf1N1B4_2370 - Malonate utilization transcriptional regulator, at 2,685,907 to 2,686,827
_2370
Pf1N1B4_2371 - COG1272: Predicted membrane protein hemolysin III homolog, at 2,687,069 to 2,687,686
_2371
Pf1N1B4_2372 - Transcriptional regulator, LysR family, at 2,687,745 to 2,688,707
_2372
Position (kb)
2685
2686
2687 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2684.999 kb on - strand at 2685.000 kb on + strand at 2685.000 kb on + strand at 2685.000 kb on + strand at 2685.000 kb on + strand at 2685.000 kb on + strand at 2685.001 kb on - strand at 2685.001 kb on - strand at 2685.035 kb on + strand at 2685.035 kb on + strand at 2685.035 kb on + strand at 2685.036 kb on - strand at 2685.036 kb on - strand at 2685.036 kb on - strand at 2685.068 kb on + strand at 2685.068 kb on + strand at 2685.477 kb on + strand, within Pf1N1B4_2369 at 2685.477 kb on + strand, within Pf1N1B4_2369 at 2685.477 kb on + strand, within Pf1N1B4_2369 at 2685.477 kb on + strand, within Pf1N1B4_2369 at 2685.478 kb on - strand, within Pf1N1B4_2369 at 2685.478 kb on - strand, within Pf1N1B4_2369 at 2685.478 kb on - strand, within Pf1N1B4_2369 at 2685.695 kb on + strand, within Pf1N1B4_2369 at 2685.771 kb on + strand at 2685.771 kb on + strand at 2685.771 kb on + strand at 2685.932 kb on + strand at 2685.932 kb on + strand at 2685.932 kb on + strand at 2685.933 kb on - strand at 2685.933 kb on - strand at 2685.933 kb on - strand at 2686.022 kb on + strand, within Pf1N1B4_2370 at 2686.061 kb on + strand, within Pf1N1B4_2370 at 2686.061 kb on + strand, within Pf1N1B4_2370 at 2686.062 kb on - strand, within Pf1N1B4_2370 at 2686.062 kb on - strand, within Pf1N1B4_2370 at 2686.192 kb on - strand, within Pf1N1B4_2370 at 2686.409 kb on + strand, within Pf1N1B4_2370 at 2686.410 kb on - strand, within Pf1N1B4_2370 at 2686.514 kb on + strand, within Pf1N1B4_2370 at 2686.515 kb on - strand, within Pf1N1B4_2370 at 2686.625 kb on + strand, within Pf1N1B4_2370 at 2686.625 kb on + strand, within Pf1N1B4_2370 at 2686.625 kb on + strand, within Pf1N1B4_2370 at 2686.625 kb on + strand, within Pf1N1B4_2370 at 2686.625 kb on + strand, within Pf1N1B4_2370 at 2686.626 kb on - strand, within Pf1N1B4_2370 at 2686.626 kb on - strand, within Pf1N1B4_2370 at 2686.626 kb on - strand, within Pf1N1B4_2370 at 2686.626 kb on - strand, within Pf1N1B4_2370 at 2686.840 kb on + strand at 2686.841 kb on - strand at 2686.949 kb on - strand at 2686.995 kb on + strand at 2686.995 kb on + strand at 2686.995 kb on + strand at 2687.006 kb on + strand at 2687.006 kb on + strand at 2687.007 kb on - strand at 2687.017 kb on + strand at 2687.017 kb on + strand at 2687.018 kb on - strand at 2687.018 kb on - strand at 2687.018 kb on - strand at 2687.028 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.066 kb on + strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.067 kb on - strand at 2687.072 kb on + strand at 2687.072 kb on + strand at 2687.072 kb on + strand at 2687.072 kb on + strand at 2687.072 kb on + strand at 2687.072 kb on + strand at 2687.072 kb on + strand at 2687.072 kb on + strand at 2687.072 kb on + strand at 2687.072 kb on + strand at 2687.073 kb on - strand at 2687.073 kb on - strand at 2687.073 kb on - strand at 2687.073 kb on - strand at 2687.201 kb on + strand, within Pf1N1B4_2371 at 2687.225 kb on + strand, within Pf1N1B4_2371 at 2687.226 kb on - strand, within Pf1N1B4_2371 at 2687.244 kb on - strand, within Pf1N1B4_2371 at 2687.244 kb on - strand, within Pf1N1B4_2371 at 2687.303 kb on + strand, within Pf1N1B4_2371 at 2687.304 kb on - strand, within Pf1N1B4_2371 at 2687.324 kb on + strand, within Pf1N1B4_2371 at 2687.325 kb on - strand, within Pf1N1B4_2371 at 2687.325 kb on - strand, within Pf1N1B4_2371 at 2687.462 kb on + strand, within Pf1N1B4_2371 at 2687.462 kb on + strand, within Pf1N1B4_2371 at 2687.463 kb on - strand, within Pf1N1B4_2371 at 2687.505 kb on + strand, within Pf1N1B4_2371 at 2687.561 kb on + strand, within Pf1N1B4_2371 at 2687.561 kb on + strand at 2687.561 kb on + strand, within Pf1N1B4_2371 at 2687.561 kb on + strand, within Pf1N1B4_2371 at 2687.561 kb on + strand, within Pf1N1B4_2371 at 2687.561 kb on + strand, within Pf1N1B4_2371 at 2687.561 kb on + strand, within Pf1N1B4_2371 at 2687.561 kb on + strand, within Pf1N1B4_2371 at 2687.561 kb on + strand, within Pf1N1B4_2371 at 2687.562 kb on - strand at 2687.562 kb on - strand, within Pf1N1B4_2371 at 2687.675 kb on + strand at 2687.675 kb on + strand at 2687.675 kb on + strand at 2687.675 kb on + strand at 2687.676 kb on - strand at 2687.676 kb on - strand at 2687.684 kb on + strand at 2687.684 kb on + strand at 2687.684 kb on + strand at 2687.700 kb on + strand at 2687.700 kb on + strand at 2687.701 kb on - strand at 2687.701 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,684,999 - +1.4 2,685,000 + +0.5 2,685,000 + +0.0 2,685,000 + -1.6 2,685,000 + -1.0 2,685,000 + -2.3 2,685,001 - -1.1 2,685,001 - -0.6 2,685,035 + -1.8 2,685,035 + +0.5 2,685,035 + +0.8 2,685,036 - -1.0 2,685,036 - +0.2 2,685,036 - +1.9 2,685,068 + -2.3 2,685,068 + -0.2 2,685,477 + Pf1N1B4_2369 0.58 -0.3 2,685,477 + Pf1N1B4_2369 0.58 -0.2 2,685,477 + Pf1N1B4_2369 0.58 +0.7 2,685,477 + Pf1N1B4_2369 0.58 -0.2 2,685,478 - Pf1N1B4_2369 0.58 +0.7 2,685,478 - Pf1N1B4_2369 0.58 +0.1 2,685,478 - Pf1N1B4_2369 0.58 -0.2 2,685,695 + Pf1N1B4_2369 0.86 -0.7 2,685,771 + -0.4 2,685,771 + -0.6 2,685,771 + +0.6 2,685,932 + +1.6 2,685,932 + -0.2 2,685,932 + +1.4 2,685,933 - -0.5 2,685,933 - -1.2 2,685,933 - +0.6 2,686,022 + Pf1N1B4_2370 0.12 -0.3 2,686,061 + Pf1N1B4_2370 0.17 +0.6 2,686,061 + Pf1N1B4_2370 0.17 +1.9 2,686,062 - Pf1N1B4_2370 0.17 -1.0 2,686,062 - Pf1N1B4_2370 0.17 +1.6 2,686,192 - Pf1N1B4_2370 0.31 -0.2 2,686,409 + Pf1N1B4_2370 0.55 +1.4 2,686,410 - Pf1N1B4_2370 0.55 -0.5 2,686,514 + Pf1N1B4_2370 0.66 +0.4 2,686,515 - Pf1N1B4_2370 0.66 +1.8 2,686,625 + Pf1N1B4_2370 0.78 -1.5 2,686,625 + Pf1N1B4_2370 0.78 +0.9 2,686,625 + Pf1N1B4_2370 0.78 +2.9 2,686,625 + Pf1N1B4_2370 0.78 -0.6 2,686,625 + Pf1N1B4_2370 0.78 -0.2 2,686,626 - Pf1N1B4_2370 0.78 +1.4 2,686,626 - Pf1N1B4_2370 0.78 -1.6 2,686,626 - Pf1N1B4_2370 0.78 -0.2 2,686,626 - Pf1N1B4_2370 0.78 +0.7 2,686,840 + +1.4 2,686,841 - -0.6 2,686,949 - -0.6 2,686,995 + +0.4 2,686,995 + +2.4 2,686,995 + +1.6 2,687,006 + +0.1 2,687,006 + +0.9 2,687,007 - -0.5 2,687,017 + +0.3 2,687,017 + +1.3 2,687,018 - -1.8 2,687,018 - -0.2 2,687,018 - +1.4 2,687,028 + -1.6 2,687,066 + +0.9 2,687,066 + -0.3 2,687,066 + +0.4 2,687,066 + +1.4 2,687,066 + -0.6 2,687,066 + -0.6 2,687,066 + -1.8 2,687,066 + -0.5 2,687,066 + +1.4 2,687,066 + -0.9 2,687,066 + -0.1 2,687,066 + +0.1 2,687,066 + -0.8 2,687,066 + -0.2 2,687,066 + +0.2 2,687,066 + +0.2 2,687,066 + +1.1 2,687,067 - +0.4 2,687,067 - -1.0 2,687,067 - -0.4 2,687,067 - -0.6 2,687,067 - -0.2 2,687,067 - +1.7 2,687,067 - -0.7 2,687,067 - -2.5 2,687,067 - -2.0 2,687,067 - +0.4 2,687,067 - -0.2 2,687,067 - +1.4 2,687,067 - -0.5 2,687,067 - +1.1 2,687,067 - +0.8 2,687,067 - -0.2 2,687,067 - -1.5 2,687,067 - -1.5 2,687,072 + +0.2 2,687,072 + +0.1 2,687,072 + +0.8 2,687,072 + -0.6 2,687,072 + +0.4 2,687,072 + -1.8 2,687,072 + +1.4 2,687,072 + -0.5 2,687,072 + +0.5 2,687,072 + -1.4 2,687,073 - -0.2 2,687,073 - +0.1 2,687,073 - -0.5 2,687,073 - +1.4 2,687,201 + Pf1N1B4_2371 0.21 -0.5 2,687,225 + Pf1N1B4_2371 0.25 -0.6 2,687,226 - Pf1N1B4_2371 0.25 -0.5 2,687,244 - Pf1N1B4_2371 0.28 -0.4 2,687,244 - Pf1N1B4_2371 0.28 +0.0 2,687,303 + Pf1N1B4_2371 0.38 +0.6 2,687,304 - Pf1N1B4_2371 0.38 +2.7 2,687,324 + Pf1N1B4_2371 0.41 -0.6 2,687,325 - Pf1N1B4_2371 0.41 +0.6 2,687,325 - Pf1N1B4_2371 0.41 -0.2 2,687,462 + Pf1N1B4_2371 0.64 +0.4 2,687,462 + Pf1N1B4_2371 0.64 -1.5 2,687,463 - Pf1N1B4_2371 0.64 -0.8 2,687,505 + Pf1N1B4_2371 0.71 -2.1 2,687,561 + Pf1N1B4_2371 0.80 +0.0 2,687,561 + +0.4 2,687,561 + Pf1N1B4_2371 0.80 +0.0 2,687,561 + Pf1N1B4_2371 0.80 -0.5 2,687,561 + Pf1N1B4_2371 0.80 -1.2 2,687,561 + Pf1N1B4_2371 0.80 -0.6 2,687,561 + Pf1N1B4_2371 0.80 -0.8 2,687,561 + Pf1N1B4_2371 0.80 +0.5 2,687,561 + Pf1N1B4_2371 0.80 +0.4 2,687,562 - +0.4 2,687,562 - Pf1N1B4_2371 0.80 -2.0 2,687,675 + -0.5 2,687,675 + +0.7 2,687,675 + +2.9 2,687,675 + -0.3 2,687,676 - -1.5 2,687,676 - +0.0 2,687,684 + +1.1 2,687,684 + +0.6 2,687,684 + -1.5 2,687,700 + -2.6 2,687,700 + -0.8 2,687,701 - +0.1 2,687,701 - -1.5
Or see this region's nucleotide sequence