Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2322

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2321 and Pf1N1B4_2322 overlap by 8 nucleotidesPf1N1B4_2322 and Pf1N1B4_2323 are separated by 155 nucleotidesPf1N1B4_2323 and Pf1N1B4_2324 are separated by 60 nucleotides Pf1N1B4_2321 - FIG015287: Zinc protease, at 2,640,800 to 2,642,290 _2321 Pf1N1B4_2322 - FIG015547: peptidase, M16 family, at 2,642,283 to 2,643,638 _2322 Pf1N1B4_2323 - hypothetical protein, at 2,643,794 to 2,643,922 _2323 Pf1N1B4_2324 - Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1), at 2,643,983 to 2,645,479 _2324 Position (kb) 2642 2643 2644Strain fitness (log2 ratio) -1 0 1at 2641.657 kb on - strand, within Pf1N1B4_2321at 2642.060 kb on + strand, within Pf1N1B4_2321at 2642.215 kb on - strandat 2642.310 kb on + strandat 2642.311 kb on - strandat 2642.311 kb on - strandat 2642.345 kb on - strandat 2642.345 kb on - strandat 2642.486 kb on - strand, within Pf1N1B4_2322at 2642.514 kb on + strand, within Pf1N1B4_2322at 2642.669 kb on - strand, within Pf1N1B4_2322at 2643.017 kb on - strand, within Pf1N1B4_2322at 2643.137 kb on - strand, within Pf1N1B4_2322at 2643.467 kb on - strand, within Pf1N1B4_2322at 2643.675 kb on - strandat 2643.928 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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2,641,657 - Pf1N1B4_2321 0.57 -0.6
2,642,060 + Pf1N1B4_2321 0.85 -1.2
2,642,215 - -1.5
2,642,310 + +0.0
2,642,311 - -0.7
2,642,311 - +1.4
2,642,345 - +1.0
2,642,345 - -1.2
2,642,486 - Pf1N1B4_2322 0.15 -1.6
2,642,514 + Pf1N1B4_2322 0.17 -0.2
2,642,669 - Pf1N1B4_2322 0.28 -0.2
2,643,017 - Pf1N1B4_2322 0.54 +0.0
2,643,137 - Pf1N1B4_2322 0.63 -0.1
2,643,467 - Pf1N1B4_2322 0.87 -1.5
2,643,675 - -0.7
2,643,928 + -1.3

Or see this region's nucleotide sequence