Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_231 and Pf1N1B4_232 are separated by 47 nucleotides Pf1N1B4_232 and Pf1N1B4_233 overlap by 17 nucleotides
Pf1N1B4_231 - Sensor histidine kinase, at 292,071 to 292,964
_231
Pf1N1B4_232 - DNA-binding response regulator, LuxR family, at 293,012 to 293,620
_232
Pf1N1B4_233 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 293,604 to 294,536
_233
Position (kb) 293
294 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 292.070 kb on + strand at 292.071 kb on - strand at 292.074 kb on + strand at 292.075 kb on - strand at 292.075 kb on - strand at 292.095 kb on - strand at 292.095 kb on - strand at 292.095 kb on - strand at 292.148 kb on + strand at 292.148 kb on + strand at 292.149 kb on - strand at 292.193 kb on - strand, within Pf1N1B4_231 at 292.224 kb on - strand, within Pf1N1B4_231 at 292.561 kb on + strand, within Pf1N1B4_231 at 292.561 kb on + strand at 292.562 kb on - strand, within Pf1N1B4_231 at 292.580 kb on + strand, within Pf1N1B4_231 at 292.580 kb on + strand, within Pf1N1B4_231 at 292.580 kb on + strand, within Pf1N1B4_231 at 292.581 kb on - strand, within Pf1N1B4_231 at 292.682 kb on + strand, within Pf1N1B4_231 at 292.682 kb on + strand, within Pf1N1B4_231 at 292.683 kb on - strand at 292.684 kb on + strand, within Pf1N1B4_231 at 292.684 kb on + strand, within Pf1N1B4_231 at 292.684 kb on + strand, within Pf1N1B4_231 at 292.706 kb on + strand, within Pf1N1B4_231 at 292.849 kb on + strand, within Pf1N1B4_231 at 292.849 kb on + strand, within Pf1N1B4_231 at 292.931 kb on - strand at 292.949 kb on + strand at 292.950 kb on - strand at 292.969 kb on + strand at 292.969 kb on + strand at 292.970 kb on - strand at 292.974 kb on + strand at 292.974 kb on + strand at 292.974 kb on + strand at 292.974 kb on + strand at 292.975 kb on - strand at 292.975 kb on - strand at 292.999 kb on + strand at 293.049 kb on + strand at 293.125 kb on + strand at 293.558 kb on + strand, within Pf1N1B4_232 at 293.559 kb on - strand, within Pf1N1B4_232 at 293.559 kb on - strand, within Pf1N1B4_232 at 293.735 kb on + strand, within Pf1N1B4_233 at 293.736 kb on - strand, within Pf1N1B4_233 at 293.736 kb on - strand at 293.737 kb on + strand at 293.738 kb on - strand at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand at 293.738 kb on - strand, within Pf1N1B4_233 at 294.046 kb on + strand, within Pf1N1B4_233 at 294.046 kb on + strand, within Pf1N1B4_233 at 294.047 kb on - strand, within Pf1N1B4_233 at 294.087 kb on - strand, within Pf1N1B4_233 at 294.087 kb on - strand, within Pf1N1B4_233 at 294.087 kb on - strand, within Pf1N1B4_233 at 294.264 kb on + strand, within Pf1N1B4_233 at 294.265 kb on - strand at 294.351 kb on - strand, within Pf1N1B4_233 at 294.555 kb on + strand at 294.555 kb on + strand at 294.555 kb on + strand at 294.556 kb on - strand at 294.556 kb on - strand at 294.578 kb on + strand at 294.579 kb on - strand at 294.579 kb on - strand at 294.600 kb on + strand at 294.601 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 292,070 + -1.5 292,071 - -0.1 292,074 + +0.3 292,075 - +0.4 292,075 - -1.3 292,095 - -0.8 292,095 - +0.7 292,095 - +2.6 292,148 + +0.7 292,148 + -0.1 292,149 - +0.2 292,193 - Pf1N1B4_231 0.14 -1.3 292,224 - Pf1N1B4_231 0.17 +0.7 292,561 + Pf1N1B4_231 0.55 -0.6 292,561 + +0.3 292,562 - Pf1N1B4_231 0.55 -1.3 292,580 + Pf1N1B4_231 0.57 +0.3 292,580 + Pf1N1B4_231 0.57 +2.3 292,580 + Pf1N1B4_231 0.57 -0.8 292,581 - Pf1N1B4_231 0.57 +0.5 292,682 + Pf1N1B4_231 0.68 -1.6 292,682 + Pf1N1B4_231 0.68 -0.3 292,683 - +1.2 292,684 + Pf1N1B4_231 0.69 -0.6 292,684 + Pf1N1B4_231 0.69 +0.6 292,684 + Pf1N1B4_231 0.69 -1.8 292,706 + Pf1N1B4_231 0.71 -2.4 292,849 + Pf1N1B4_231 0.87 +0.9 292,849 + Pf1N1B4_231 0.87 -0.5 292,931 - -1.6 292,949 + -1.1 292,950 - -0.4 292,969 + -1.3 292,969 + -1.3 292,970 - +0.1 292,974 + -0.3 292,974 + +0.7 292,974 + -1.9 292,974 + -0.3 292,975 - +0.5 292,975 - -0.1 292,999 + +0.5 293,049 + -0.3 293,125 + -0.8 293,558 + Pf1N1B4_232 0.90 +0.7 293,559 - Pf1N1B4_232 0.90 +1.7 293,559 - Pf1N1B4_232 0.90 -0.0 293,735 + Pf1N1B4_233 0.14 +0.3 293,736 - Pf1N1B4_233 0.14 -0.2 293,736 - -0.2 293,737 + +2.8 293,738 - +1.3 293,738 - Pf1N1B4_233 0.14 +0.0 293,738 - Pf1N1B4_233 0.14 -0.6 293,738 - Pf1N1B4_233 0.14 -1.7 293,738 - Pf1N1B4_233 0.14 -2.6 293,738 - -0.2 293,738 - Pf1N1B4_233 0.14 +0.2 294,046 + Pf1N1B4_233 0.47 +0.9 294,046 + Pf1N1B4_233 0.47 -0.8 294,047 - Pf1N1B4_233 0.47 +0.8 294,087 - Pf1N1B4_233 0.52 +1.6 294,087 - Pf1N1B4_233 0.52 +0.5 294,087 - Pf1N1B4_233 0.52 +0.8 294,264 + Pf1N1B4_233 0.71 +0.0 294,265 - +1.3 294,351 - Pf1N1B4_233 0.80 +0.3 294,555 + -0.5 294,555 + -2.4 294,555 + -0.8 294,556 - -2.2 294,556 - +0.5 294,578 + -2.1 294,579 - -0.7 294,579 - +1.2 294,600 + +1.3 294,601 - +0.4
Or see this region's nucleotide sequence