Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2248 and Pf1N1B4_2249 are separated by 25 nucleotides Pf1N1B4_2249 and Pf1N1B4_2250 are separated by 9 nucleotides Pf1N1B4_2250 and Pf1N1B4_2251 overlap by 4 nucleotides
Pf1N1B4_2248 - Alkanesulfonates-binding protein, at 2,564,582 to 2,565,547
_2248
Pf1N1B4_2249 - Alkanesulfonate monooxygenase (EC 1.14.14.5), at 2,565,573 to 2,566,721
_2249
Pf1N1B4_2250 - Alkanesulfonates transport system permease protein, at 2,566,731 to 2,567,513
_2250
Pf1N1B4_2251 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 2,567,510 to 2,568,316
_2251
Position (kb)
2565
2566
2567 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2564.634 kb on + strand at 2564.650 kb on + strand at 2564.693 kb on + strand, within Pf1N1B4_2248 at 2564.694 kb on - strand, within Pf1N1B4_2248 at 2564.888 kb on + strand, within Pf1N1B4_2248 at 2564.888 kb on + strand, within Pf1N1B4_2248 at 2564.888 kb on + strand, within Pf1N1B4_2248 at 2564.888 kb on + strand, within Pf1N1B4_2248 at 2564.888 kb on + strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2565.014 kb on + strand, within Pf1N1B4_2248 at 2565.014 kb on + strand, within Pf1N1B4_2248 at 2565.014 kb on + strand, within Pf1N1B4_2248 at 2565.014 kb on + strand, within Pf1N1B4_2248 at 2565.014 kb on + strand, within Pf1N1B4_2248 at 2565.014 kb on + strand, within Pf1N1B4_2248 at 2565.015 kb on - strand, within Pf1N1B4_2248 at 2565.015 kb on - strand at 2565.053 kb on + strand, within Pf1N1B4_2248 at 2565.053 kb on + strand, within Pf1N1B4_2248 at 2565.053 kb on + strand, within Pf1N1B4_2248 at 2565.054 kb on - strand, within Pf1N1B4_2248 at 2565.054 kb on - strand, within Pf1N1B4_2248 at 2565.054 kb on - strand, within Pf1N1B4_2248 at 2565.146 kb on + strand, within Pf1N1B4_2248 at 2565.224 kb on + strand, within Pf1N1B4_2248 at 2565.225 kb on - strand, within Pf1N1B4_2248 at 2565.225 kb on - strand, within Pf1N1B4_2248 at 2565.225 kb on - strand, within Pf1N1B4_2248 at 2565.242 kb on + strand, within Pf1N1B4_2248 at 2565.242 kb on + strand, within Pf1N1B4_2248 at 2565.243 kb on - strand, within Pf1N1B4_2248 at 2565.243 kb on - strand, within Pf1N1B4_2248 at 2565.243 kb on - strand, within Pf1N1B4_2248 at 2565.418 kb on + strand, within Pf1N1B4_2248 at 2565.429 kb on + strand, within Pf1N1B4_2248 at 2565.429 kb on + strand, within Pf1N1B4_2248 at 2565.429 kb on + strand, within Pf1N1B4_2248 at 2565.430 kb on - strand, within Pf1N1B4_2248 at 2565.430 kb on - strand, within Pf1N1B4_2248 at 2565.464 kb on + strand at 2565.465 kb on - strand at 2565.465 kb on - strand at 2565.465 kb on - strand at 2565.545 kb on + strand at 2565.545 kb on + strand at 2565.546 kb on - strand at 2565.584 kb on + strand at 2565.585 kb on - strand at 2565.603 kb on - strand at 2566.020 kb on + strand, within Pf1N1B4_2249 at 2566.021 kb on - strand, within Pf1N1B4_2249 at 2566.063 kb on - strand, within Pf1N1B4_2249 at 2566.547 kb on + strand, within Pf1N1B4_2249 at 2566.547 kb on + strand, within Pf1N1B4_2249 at 2566.547 kb on + strand, within Pf1N1B4_2249 at 2566.548 kb on - strand, within Pf1N1B4_2249 at 2566.548 kb on - strand, within Pf1N1B4_2249 at 2566.606 kb on + strand, within Pf1N1B4_2249 at 2566.606 kb on + strand, within Pf1N1B4_2249 at 2566.607 kb on - strand at 2566.657 kb on - strand at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.962 kb on - strand, within Pf1N1B4_2250 at 2567.136 kb on + strand, within Pf1N1B4_2250 at 2567.136 kb on + strand, within Pf1N1B4_2250 at 2567.137 kb on - strand, within Pf1N1B4_2250 at 2567.137 kb on - strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.149 kb on - strand, within Pf1N1B4_2250 at 2567.149 kb on - strand, within Pf1N1B4_2250 at 2567.149 kb on - strand, within Pf1N1B4_2250 at 2567.149 kb on - strand, within Pf1N1B4_2250 at 2567.199 kb on + strand, within Pf1N1B4_2250 at 2567.199 kb on + strand, within Pf1N1B4_2250 at 2567.199 kb on + strand, within Pf1N1B4_2250 at 2567.200 kb on - strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.671 kb on + strand, within Pf1N1B4_2251 at 2567.671 kb on + strand, within Pf1N1B4_2251 at 2567.672 kb on - strand, within Pf1N1B4_2251
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,564,634 + -0.6 2,564,650 + -0.6 2,564,693 + Pf1N1B4_2248 0.11 +0.4 2,564,694 - Pf1N1B4_2248 0.12 +2.4 2,564,888 + Pf1N1B4_2248 0.32 -2.7 2,564,888 + Pf1N1B4_2248 0.32 -0.5 2,564,888 + Pf1N1B4_2248 0.32 +0.6 2,564,888 + Pf1N1B4_2248 0.32 +1.4 2,564,888 + Pf1N1B4_2248 0.32 +0.4 2,564,889 - Pf1N1B4_2248 0.32 +0.8 2,564,889 - Pf1N1B4_2248 0.32 +0.0 2,564,889 - +1.4 2,564,889 - Pf1N1B4_2248 0.32 -0.2 2,564,889 - Pf1N1B4_2248 0.32 -1.8 2,564,889 - Pf1N1B4_2248 0.32 +0.9 2,564,889 - Pf1N1B4_2248 0.32 -1.4 2,564,889 - Pf1N1B4_2248 0.32 +0.6 2,564,889 - Pf1N1B4_2248 0.32 -0.3 2,564,889 - Pf1N1B4_2248 0.32 -1.1 2,564,889 - Pf1N1B4_2248 0.32 +3.4 2,565,014 + Pf1N1B4_2248 0.45 -1.4 2,565,014 + Pf1N1B4_2248 0.45 +1.0 2,565,014 + Pf1N1B4_2248 0.45 +1.4 2,565,014 + Pf1N1B4_2248 0.45 -0.4 2,565,014 + Pf1N1B4_2248 0.45 +0.0 2,565,014 + Pf1N1B4_2248 0.45 +0.0 2,565,015 - Pf1N1B4_2248 0.45 -1.3 2,565,015 - -0.6 2,565,053 + Pf1N1B4_2248 0.49 +0.1 2,565,053 + Pf1N1B4_2248 0.49 -0.2 2,565,053 + Pf1N1B4_2248 0.49 +0.8 2,565,054 - Pf1N1B4_2248 0.49 +0.4 2,565,054 - Pf1N1B4_2248 0.49 -1.4 2,565,054 - Pf1N1B4_2248 0.49 -0.1 2,565,146 + Pf1N1B4_2248 0.58 -1.0 2,565,224 + Pf1N1B4_2248 0.66 -0.6 2,565,225 - Pf1N1B4_2248 0.67 +0.6 2,565,225 - Pf1N1B4_2248 0.67 +0.9 2,565,225 - Pf1N1B4_2248 0.67 +0.5 2,565,242 + Pf1N1B4_2248 0.68 +1.8 2,565,242 + Pf1N1B4_2248 0.68 +0.8 2,565,243 - Pf1N1B4_2248 0.68 +0.4 2,565,243 - Pf1N1B4_2248 0.68 +0.0 2,565,243 - Pf1N1B4_2248 0.68 +0.8 2,565,418 + Pf1N1B4_2248 0.87 +0.8 2,565,429 + Pf1N1B4_2248 0.88 -0.2 2,565,429 + Pf1N1B4_2248 0.88 -0.6 2,565,429 + Pf1N1B4_2248 0.88 -1.7 2,565,430 - Pf1N1B4_2248 0.88 +0.8 2,565,430 - Pf1N1B4_2248 0.88 +0.4 2,565,464 + -0.2 2,565,465 - +1.1 2,565,465 - +0.4 2,565,465 - -0.6 2,565,545 + +1.3 2,565,545 + +1.5 2,565,546 - -1.8 2,565,584 + +0.9 2,565,585 - -1.3 2,565,603 - -1.0 2,566,020 + Pf1N1B4_2249 0.39 -2.1 2,566,021 - Pf1N1B4_2249 0.39 +0.4 2,566,063 - Pf1N1B4_2249 0.43 -0.6 2,566,547 + Pf1N1B4_2249 0.85 -1.2 2,566,547 + Pf1N1B4_2249 0.85 -1.0 2,566,547 + Pf1N1B4_2249 0.85 +0.8 2,566,548 - Pf1N1B4_2249 0.85 +0.0 2,566,548 - Pf1N1B4_2249 0.85 -1.2 2,566,606 + Pf1N1B4_2249 0.90 -1.0 2,566,606 + Pf1N1B4_2249 0.90 -2.6 2,566,607 - -0.1 2,566,657 - -0.0 2,566,961 + Pf1N1B4_2250 0.29 -0.8 2,566,961 + Pf1N1B4_2250 0.29 +0.0 2,566,961 + Pf1N1B4_2250 0.29 -0.7 2,566,961 + Pf1N1B4_2250 0.29 +0.6 2,566,961 + Pf1N1B4_2250 0.29 +0.5 2,566,961 + Pf1N1B4_2250 0.29 +0.8 2,566,961 + Pf1N1B4_2250 0.29 +0.4 2,566,962 - Pf1N1B4_2250 0.30 -0.2 2,567,136 + Pf1N1B4_2250 0.52 +0.1 2,567,136 + Pf1N1B4_2250 0.52 -0.6 2,567,137 - Pf1N1B4_2250 0.52 -0.2 2,567,137 - Pf1N1B4_2250 0.52 -0.5 2,567,148 + Pf1N1B4_2250 0.53 +1.6 2,567,148 + Pf1N1B4_2250 0.53 +0.1 2,567,148 + Pf1N1B4_2250 0.53 -0.0 2,567,148 + Pf1N1B4_2250 0.53 -0.4 2,567,148 + Pf1N1B4_2250 0.53 -0.2 2,567,148 + Pf1N1B4_2250 0.53 -0.3 2,567,148 + Pf1N1B4_2250 0.53 -0.7 2,567,149 - Pf1N1B4_2250 0.53 -2.2 2,567,149 - Pf1N1B4_2250 0.53 +1.0 2,567,149 - Pf1N1B4_2250 0.53 +0.4 2,567,149 - Pf1N1B4_2250 0.53 +0.8 2,567,199 + Pf1N1B4_2250 0.60 +0.8 2,567,199 + Pf1N1B4_2250 0.60 +0.4 2,567,199 + Pf1N1B4_2250 0.60 -1.0 2,567,200 - Pf1N1B4_2250 0.60 +2.1 2,567,409 + Pf1N1B4_2250 0.87 -1.0 2,567,409 + Pf1N1B4_2250 0.87 -1.0 2,567,409 + Pf1N1B4_2250 0.87 -0.2 2,567,671 + Pf1N1B4_2251 0.20 +1.6 2,567,671 + Pf1N1B4_2251 0.20 +0.7 2,567,672 - Pf1N1B4_2251 0.20 -0.4
Or see this region's nucleotide sequence