Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2249

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2248 and Pf1N1B4_2249 are separated by 25 nucleotidesPf1N1B4_2249 and Pf1N1B4_2250 are separated by 9 nucleotidesPf1N1B4_2250 and Pf1N1B4_2251 overlap by 4 nucleotides Pf1N1B4_2248 - Alkanesulfonates-binding protein, at 2,564,582 to 2,565,547 _2248 Pf1N1B4_2249 - Alkanesulfonate monooxygenase (EC 1.14.14.5), at 2,565,573 to 2,566,721 _2249 Pf1N1B4_2250 - Alkanesulfonates transport system permease protein, at 2,566,731 to 2,567,513 _2250 Pf1N1B4_2251 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 2,567,510 to 2,568,316 _2251 Position (kb) 2565 2566 2567Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2564.634 kb on + strandat 2564.650 kb on + strandat 2564.693 kb on + strand, within Pf1N1B4_2248at 2564.694 kb on - strand, within Pf1N1B4_2248at 2564.888 kb on + strand, within Pf1N1B4_2248at 2564.888 kb on + strand, within Pf1N1B4_2248at 2564.888 kb on + strand, within Pf1N1B4_2248at 2564.888 kb on + strand, within Pf1N1B4_2248at 2564.888 kb on + strand, within Pf1N1B4_2248at 2564.889 kb on - strand, within Pf1N1B4_2248at 2564.889 kb on - strand, within Pf1N1B4_2248at 2564.889 kb on - strandat 2564.889 kb on - strand, within Pf1N1B4_2248at 2564.889 kb on - strand, within Pf1N1B4_2248at 2564.889 kb on - strand, within Pf1N1B4_2248at 2564.889 kb on - strand, within Pf1N1B4_2248at 2564.889 kb on - strand, within Pf1N1B4_2248at 2564.889 kb on - strand, within Pf1N1B4_2248at 2564.889 kb on - strand, within Pf1N1B4_2248at 2564.889 kb on - strand, within Pf1N1B4_2248at 2565.014 kb on + strand, within Pf1N1B4_2248at 2565.014 kb on + strand, within Pf1N1B4_2248at 2565.014 kb on + strand, within Pf1N1B4_2248at 2565.014 kb on + strand, within Pf1N1B4_2248at 2565.014 kb on + strand, within Pf1N1B4_2248at 2565.014 kb on + strand, within Pf1N1B4_2248at 2565.015 kb on - strand, within Pf1N1B4_2248at 2565.015 kb on - strandat 2565.053 kb on + strand, within Pf1N1B4_2248at 2565.053 kb on + strand, within Pf1N1B4_2248at 2565.053 kb on + strand, within Pf1N1B4_2248at 2565.054 kb on - strand, within Pf1N1B4_2248at 2565.054 kb on - strand, within Pf1N1B4_2248at 2565.054 kb on - strand, within Pf1N1B4_2248at 2565.146 kb on + strand, within Pf1N1B4_2248at 2565.224 kb on + strand, within Pf1N1B4_2248at 2565.225 kb on - strand, within Pf1N1B4_2248at 2565.225 kb on - strand, within Pf1N1B4_2248at 2565.225 kb on - strand, within Pf1N1B4_2248at 2565.242 kb on + strand, within Pf1N1B4_2248at 2565.242 kb on + strand, within Pf1N1B4_2248at 2565.243 kb on - strand, within Pf1N1B4_2248at 2565.243 kb on - strand, within Pf1N1B4_2248at 2565.243 kb on - strand, within Pf1N1B4_2248at 2565.418 kb on + strand, within Pf1N1B4_2248at 2565.429 kb on + strand, within Pf1N1B4_2248at 2565.429 kb on + strand, within Pf1N1B4_2248at 2565.429 kb on + strand, within Pf1N1B4_2248at 2565.430 kb on - strand, within Pf1N1B4_2248at 2565.430 kb on - strand, within Pf1N1B4_2248at 2565.464 kb on + strandat 2565.465 kb on - strandat 2565.465 kb on - strandat 2565.465 kb on - strandat 2565.545 kb on + strandat 2565.545 kb on + strandat 2565.546 kb on - strandat 2565.584 kb on + strandat 2565.585 kb on - strandat 2565.603 kb on - strandat 2566.020 kb on + strand, within Pf1N1B4_2249at 2566.021 kb on - strand, within Pf1N1B4_2249at 2566.063 kb on - strand, within Pf1N1B4_2249at 2566.547 kb on + strand, within Pf1N1B4_2249at 2566.547 kb on + strand, within Pf1N1B4_2249at 2566.547 kb on + strand, within Pf1N1B4_2249at 2566.548 kb on - strand, within Pf1N1B4_2249at 2566.548 kb on - strand, within Pf1N1B4_2249at 2566.606 kb on + strand, within Pf1N1B4_2249at 2566.606 kb on + strand, within Pf1N1B4_2249at 2566.607 kb on - strandat 2566.657 kb on - strandat 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.962 kb on - strand, within Pf1N1B4_2250at 2567.136 kb on + strand, within Pf1N1B4_2250at 2567.136 kb on + strand, within Pf1N1B4_2250at 2567.137 kb on - strand, within Pf1N1B4_2250at 2567.137 kb on - strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.149 kb on - strand, within Pf1N1B4_2250at 2567.149 kb on - strand, within Pf1N1B4_2250at 2567.149 kb on - strand, within Pf1N1B4_2250at 2567.149 kb on - strand, within Pf1N1B4_2250at 2567.199 kb on + strand, within Pf1N1B4_2250at 2567.199 kb on + strand, within Pf1N1B4_2250at 2567.199 kb on + strand, within Pf1N1B4_2250at 2567.200 kb on - strand, within Pf1N1B4_2250at 2567.409 kb on + strand, within Pf1N1B4_2250at 2567.409 kb on + strand, within Pf1N1B4_2250at 2567.409 kb on + strand, within Pf1N1B4_2250at 2567.671 kb on + strand, within Pf1N1B4_2251at 2567.671 kb on + strand, within Pf1N1B4_2251at 2567.672 kb on - strand, within Pf1N1B4_2251

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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2,564,634 + -0.6
2,564,650 + -0.6
2,564,693 + Pf1N1B4_2248 0.11 +0.4
2,564,694 - Pf1N1B4_2248 0.12 +2.4
2,564,888 + Pf1N1B4_2248 0.32 -2.7
2,564,888 + Pf1N1B4_2248 0.32 -0.5
2,564,888 + Pf1N1B4_2248 0.32 +0.6
2,564,888 + Pf1N1B4_2248 0.32 +1.4
2,564,888 + Pf1N1B4_2248 0.32 +0.4
2,564,889 - Pf1N1B4_2248 0.32 +0.8
2,564,889 - Pf1N1B4_2248 0.32 +0.0
2,564,889 - +1.4
2,564,889 - Pf1N1B4_2248 0.32 -0.2
2,564,889 - Pf1N1B4_2248 0.32 -1.8
2,564,889 - Pf1N1B4_2248 0.32 +0.9
2,564,889 - Pf1N1B4_2248 0.32 -1.4
2,564,889 - Pf1N1B4_2248 0.32 +0.6
2,564,889 - Pf1N1B4_2248 0.32 -0.3
2,564,889 - Pf1N1B4_2248 0.32 -1.1
2,564,889 - Pf1N1B4_2248 0.32 +3.4
2,565,014 + Pf1N1B4_2248 0.45 -1.4
2,565,014 + Pf1N1B4_2248 0.45 +1.0
2,565,014 + Pf1N1B4_2248 0.45 +1.4
2,565,014 + Pf1N1B4_2248 0.45 -0.4
2,565,014 + Pf1N1B4_2248 0.45 +0.0
2,565,014 + Pf1N1B4_2248 0.45 +0.0
2,565,015 - Pf1N1B4_2248 0.45 -1.3
2,565,015 - -0.6
2,565,053 + Pf1N1B4_2248 0.49 +0.1
2,565,053 + Pf1N1B4_2248 0.49 -0.2
2,565,053 + Pf1N1B4_2248 0.49 +0.8
2,565,054 - Pf1N1B4_2248 0.49 +0.4
2,565,054 - Pf1N1B4_2248 0.49 -1.4
2,565,054 - Pf1N1B4_2248 0.49 -0.1
2,565,146 + Pf1N1B4_2248 0.58 -1.0
2,565,224 + Pf1N1B4_2248 0.66 -0.6
2,565,225 - Pf1N1B4_2248 0.67 +0.6
2,565,225 - Pf1N1B4_2248 0.67 +0.9
2,565,225 - Pf1N1B4_2248 0.67 +0.5
2,565,242 + Pf1N1B4_2248 0.68 +1.8
2,565,242 + Pf1N1B4_2248 0.68 +0.8
2,565,243 - Pf1N1B4_2248 0.68 +0.4
2,565,243 - Pf1N1B4_2248 0.68 +0.0
2,565,243 - Pf1N1B4_2248 0.68 +0.8
2,565,418 + Pf1N1B4_2248 0.87 +0.8
2,565,429 + Pf1N1B4_2248 0.88 -0.2
2,565,429 + Pf1N1B4_2248 0.88 -0.6
2,565,429 + Pf1N1B4_2248 0.88 -1.7
2,565,430 - Pf1N1B4_2248 0.88 +0.8
2,565,430 - Pf1N1B4_2248 0.88 +0.4
2,565,464 + -0.2
2,565,465 - +1.1
2,565,465 - +0.4
2,565,465 - -0.6
2,565,545 + +1.3
2,565,545 + +1.5
2,565,546 - -1.8
2,565,584 + +0.9
2,565,585 - -1.3
2,565,603 - -1.0
2,566,020 + Pf1N1B4_2249 0.39 -2.1
2,566,021 - Pf1N1B4_2249 0.39 +0.4
2,566,063 - Pf1N1B4_2249 0.43 -0.6
2,566,547 + Pf1N1B4_2249 0.85 -1.2
2,566,547 + Pf1N1B4_2249 0.85 -1.0
2,566,547 + Pf1N1B4_2249 0.85 +0.8
2,566,548 - Pf1N1B4_2249 0.85 +0.0
2,566,548 - Pf1N1B4_2249 0.85 -1.2
2,566,606 + Pf1N1B4_2249 0.90 -1.0
2,566,606 + Pf1N1B4_2249 0.90 -2.6
2,566,607 - -0.1
2,566,657 - -0.0
2,566,961 + Pf1N1B4_2250 0.29 -0.8
2,566,961 + Pf1N1B4_2250 0.29 +0.0
2,566,961 + Pf1N1B4_2250 0.29 -0.7
2,566,961 + Pf1N1B4_2250 0.29 +0.6
2,566,961 + Pf1N1B4_2250 0.29 +0.5
2,566,961 + Pf1N1B4_2250 0.29 +0.8
2,566,961 + Pf1N1B4_2250 0.29 +0.4
2,566,962 - Pf1N1B4_2250 0.30 -0.2
2,567,136 + Pf1N1B4_2250 0.52 +0.1
2,567,136 + Pf1N1B4_2250 0.52 -0.6
2,567,137 - Pf1N1B4_2250 0.52 -0.2
2,567,137 - Pf1N1B4_2250 0.52 -0.5
2,567,148 + Pf1N1B4_2250 0.53 +1.6
2,567,148 + Pf1N1B4_2250 0.53 +0.1
2,567,148 + Pf1N1B4_2250 0.53 -0.0
2,567,148 + Pf1N1B4_2250 0.53 -0.4
2,567,148 + Pf1N1B4_2250 0.53 -0.2
2,567,148 + Pf1N1B4_2250 0.53 -0.3
2,567,148 + Pf1N1B4_2250 0.53 -0.7
2,567,149 - Pf1N1B4_2250 0.53 -2.2
2,567,149 - Pf1N1B4_2250 0.53 +1.0
2,567,149 - Pf1N1B4_2250 0.53 +0.4
2,567,149 - Pf1N1B4_2250 0.53 +0.8
2,567,199 + Pf1N1B4_2250 0.60 +0.8
2,567,199 + Pf1N1B4_2250 0.60 +0.4
2,567,199 + Pf1N1B4_2250 0.60 -1.0
2,567,200 - Pf1N1B4_2250 0.60 +2.1
2,567,409 + Pf1N1B4_2250 0.87 -1.0
2,567,409 + Pf1N1B4_2250 0.87 -1.0
2,567,409 + Pf1N1B4_2250 0.87 -0.2
2,567,671 + Pf1N1B4_2251 0.20 +1.6
2,567,671 + Pf1N1B4_2251 0.20 +0.7
2,567,672 - Pf1N1B4_2251 0.20 -0.4

Or see this region's nucleotide sequence