Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2240

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	1-ethyl-3-methylimidazolium lysine 5 mM
2,553,844	+		Pf1N1B4_2238	0.82	+0.9
2,553,844	+		Pf1N1B4_2238	0.82	-1.4
2,553,844	+		Pf1N1B4_2238	0.82	-1.0
2,553,845	-		Pf1N1B4_2238	0.82	-0.7
2,553,901	+				+1.4
2,553,901	+		Pf1N1B4_2238	0.86	+1.6
2,553,901	+		Pf1N1B4_2238	0.86	+0.3
2,553,901	+		Pf1N1B4_2238	0.86	+2.6
2,553,901	+		Pf1N1B4_2238	0.86	-0.4
2,553,901	+		Pf1N1B4_2238	0.86	+1.0
2,553,902	-		Pf1N1B4_2238	0.86	-0.6
2,553,902	-		Pf1N1B4_2238	0.86	-1.0
2,554,026	+				+0.4
2,554,026	+				-0.4
2,554,026	+				+0.0
2,554,026	+				-1.0
2,554,027	-				-0.2
2,554,027	-				+1.4
2,554,168	+				+0.0
2,554,168	+				-1.0
2,554,169	-				+0.1
2,554,326	-		Pf1N1B4_2239	0.23	+0.0
2,554,326	-		Pf1N1B4_2239	0.23	-0.2
2,554,329	+		Pf1N1B4_2239	0.24	+0.1
2,554,330	-		Pf1N1B4_2239	0.24	+0.2
2,554,330	-		Pf1N1B4_2239	0.24	+1.4
2,554,497	+		Pf1N1B4_2239	0.73	+0.9
2,554,497	+		Pf1N1B4_2239	0.73	-3.0
2,554,498	-		Pf1N1B4_2239	0.74	-0.8
2,554,498	-		Pf1N1B4_2239	0.74	-2.1
2,554,498	-		Pf1N1B4_2239	0.74	-0.5
2,554,498	-		Pf1N1B4_2239	0.74	-0.2
2,554,498	-		Pf1N1B4_2239	0.74	+1.6
2,554,498	-		Pf1N1B4_2239	0.74	+0.4
2,554,504	+		Pf1N1B4_2239	0.76	+0.3
2,554,504	+		Pf1N1B4_2239	0.76	-0.0
2,554,575	+				+0.7
2,554,575	+				+2.4
2,554,575	+				-0.5
2,554,575	+				+1.4
2,554,576	-				-0.6
2,554,641	+				+0.9
2,554,645	+				-0.1
2,554,645	+				+0.2
2,554,645	+				-0.4
2,554,646	-				-1.1
2,554,646	-				-1.1
2,554,691	-				-0.1
2,554,691	-				-1.2
2,554,702	+				+0.6
2,554,702	+				-1.6
2,554,712	+				-1.4
2,555,010	+		Pf1N1B4_2240	0.64	+0.2
2,555,011	-		Pf1N1B4_2240	0.64	+0.2
2,555,011	-		Pf1N1B4_2240	0.64	-0.4
2,555,011	-		Pf1N1B4_2240	0.64	+0.4
2,555,070	+		Pf1N1B4_2240	0.77	+2.1
2,555,070	+		Pf1N1B4_2240	0.77	+1.7
2,555,070	+		Pf1N1B4_2240	0.77	+0.4
2,555,070	+		Pf1N1B4_2240	0.77	+0.8
2,555,071	-		Pf1N1B4_2240	0.77	+1.0
2,555,071	-		Pf1N1B4_2240	0.77	-0.2
2,555,250	+				-2.2
2,555,250	+				-0.6
2,555,250	+				-1.2
2,555,303	+				-0.6
2,555,303	+				-0.2
2,555,304	-				-2.4
2,555,339	+				+2.4
2,555,339	+				-0.2
2,555,340	-				-0.4
2,555,429	+				+1.0
2,555,430	-				+1.4
2,555,430	-				-0.2
2,555,430	-				-1.2
2,555,430	-				+0.6
2,555,742	+		Pf1N1B4_2241	0.32	-0.8
2,555,742	+		Pf1N1B4_2241	0.32	-1.0
2,555,742	+		Pf1N1B4_2241	0.32	-1.0
2,555,743	-		Pf1N1B4_2241	0.32	+0.4
2,555,743	-				+0.8
2,555,746	+		Pf1N1B4_2241	0.32	+0.6
2,555,746	+				-0.2
2,555,746	+		Pf1N1B4_2241	0.32	+0.4
2,555,746	+		Pf1N1B4_2241	0.32	+0.6
2,555,746	+		Pf1N1B4_2241	0.32	-0.2
2,555,746	+		Pf1N1B4_2241	0.32	-0.8
2,555,747	-		Pf1N1B4_2241	0.33	+0.4
2,555,747	-		Pf1N1B4_2241	0.33	-0.2
2,555,747	-		Pf1N1B4_2241	0.33	-0.6
2,555,747	-		Pf1N1B4_2241	0.33	+0.1
2,555,747	-		Pf1N1B4_2241	0.33	+0.6
2,555,747	-		Pf1N1B4_2241	0.33	-0.4
2,555,773	+		Pf1N1B4_2241	0.34	+0.4
2,555,774	-		Pf1N1B4_2241	0.35	+0.1
2,555,774	-		Pf1N1B4_2241	0.35	+1.0
2,555,933	+		Pf1N1B4_2241	0.46	-0.6
2,556,044	+		Pf1N1B4_2241	0.54	+0.6
2,556,045	-		Pf1N1B4_2241	0.54	+0.8
2,556,045	-		Pf1N1B4_2241	0.54	+0.2
2,556,139	+		Pf1N1B4_2241	0.61	-0.2
2,556,140	-		Pf1N1B4_2241	0.61	+0.0
2,556,140	-		Pf1N1B4_2241	0.61	+2.0