Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2225 and Pf1N1B4_2226 are separated by 294 nucleotides Pf1N1B4_2226 and Pf1N1B4_2227 overlap by 4 nucleotides Pf1N1B4_2227 and Pf1N1B4_2228 are separated by 126 nucleotides Pf1N1B4_2228 and Pf1N1B4_2229 are separated by 27 nucleotides
Pf1N1B4_2225 - 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2), at 2,537,856 to 2,538,461
_2225
Pf1N1B4_2226 - Z-ring-associated protein ZapA, at 2,538,756 to 2,539,073
_2226
Pf1N1B4_2227 - FIG019278: hypothetical protein, at 2,539,070 to 2,539,279
_2227
Pf1N1B4_2228 - FIG001590: Putative conserved exported protein precursor, at 2,539,406 to 2,539,960
_2228
Pf1N1B4_2229 - Xaa-Pro aminopeptidase (EC 3.4.11.9), at 2,539,988 to 2,541,322
_2229
Position (kb)
2539
2540 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2538.487 kb on - strand at 2538.524 kb on + strand at 2538.524 kb on + strand at 2538.524 kb on + strand at 2538.525 kb on - strand at 2538.525 kb on - strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.546 kb on - strand at 2538.546 kb on - strand at 2538.575 kb on + strand at 2538.576 kb on - strand at 2538.613 kb on + strand at 2538.613 kb on + strand at 2538.614 kb on - strand at 2538.614 kb on - strand at 2538.635 kb on - strand at 2538.733 kb on + strand at 2538.733 kb on + strand at 2538.733 kb on + strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.735 kb on + strand at 2538.735 kb on + strand at 2538.736 kb on - strand at 2539.188 kb on - strand, within Pf1N1B4_2227 at 2539.188 kb on - strand at 2539.191 kb on - strand, within Pf1N1B4_2227 at 2539.191 kb on - strand, within Pf1N1B4_2227 at 2539.191 kb on - strand, within Pf1N1B4_2227 at 2539.191 kb on - strand, within Pf1N1B4_2227 at 2539.212 kb on - strand, within Pf1N1B4_2227 at 2539.212 kb on - strand, within Pf1N1B4_2227 at 2539.310 kb on - strand at 2539.310 kb on - strand at 2539.310 kb on - strand at 2539.310 kb on - strand at 2539.310 kb on - strand at 2539.310 kb on - strand at 2539.310 kb on - strand at 2539.328 kb on - strand at 2539.328 kb on - strand at 2539.328 kb on - strand at 2539.328 kb on - strand at 2539.331 kb on - strand at 2539.331 kb on - strand at 2539.331 kb on - strand at 2539.331 kb on - strand at 2539.331 kb on - strand at 2539.331 kb on - strand at 2539.353 kb on + strand at 2539.354 kb on - strand at 2539.354 kb on - strand at 2539.354 kb on - strand at 2539.354 kb on - strand at 2539.354 kb on - strand at 2539.354 kb on - strand at 2539.354 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.375 kb on - strand at 2539.375 kb on - strand at 2539.375 kb on - strand at 2539.375 kb on - strand at 2539.375 kb on - strand at 2539.381 kb on - strand at 2539.381 kb on - strand at 2539.381 kb on - strand at 2539.398 kb on - strand at 2539.398 kb on - strand at 2539.636 kb on + strand, within Pf1N1B4_2228 at 2539.637 kb on - strand, within Pf1N1B4_2228 at 2539.637 kb on - strand, within Pf1N1B4_2228 at 2539.763 kb on + strand, within Pf1N1B4_2228 at 2539.784 kb on - strand, within Pf1N1B4_2228 at 2539.862 kb on + strand at 2539.863 kb on - strand, within Pf1N1B4_2228 at 2539.863 kb on - strand, within Pf1N1B4_2228 at 2539.863 kb on - strand, within Pf1N1B4_2228 at 2539.880 kb on + strand, within Pf1N1B4_2228 at 2539.880 kb on + strand, within Pf1N1B4_2228 at 2539.881 kb on - strand, within Pf1N1B4_2228 at 2539.881 kb on - strand, within Pf1N1B4_2228 at 2539.881 kb on - strand, within Pf1N1B4_2228 at 2539.881 kb on - strand, within Pf1N1B4_2228 at 2539.958 kb on + strand at 2539.996 kb on + strand at 2540.012 kb on + strand at 2540.012 kb on + strand at 2540.141 kb on + strand, within Pf1N1B4_2229 at 2540.141 kb on + strand, within Pf1N1B4_2229 at 2540.142 kb on - strand, within Pf1N1B4_2229 at 2540.207 kb on + strand, within Pf1N1B4_2229 at 2540.207 kb on + strand, within Pf1N1B4_2229 at 2540.207 kb on + strand, within Pf1N1B4_2229 at 2540.207 kb on + strand, within Pf1N1B4_2229 at 2540.208 kb on - strand, within Pf1N1B4_2229 at 2540.208 kb on - strand, within Pf1N1B4_2229 at 2540.231 kb on - strand, within Pf1N1B4_2229 at 2540.369 kb on + strand, within Pf1N1B4_2229 at 2540.369 kb on + strand, within Pf1N1B4_2229 at 2540.370 kb on - strand, within Pf1N1B4_2229 at 2540.370 kb on - strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.472 kb on - strand, within Pf1N1B4_2229 at 2540.472 kb on - strand, within Pf1N1B4_2229 at 2540.472 kb on - strand, within Pf1N1B4_2229 at 2540.472 kb on - strand, within Pf1N1B4_2229 at 2540.510 kb on + strand, within Pf1N1B4_2229 at 2540.511 kb on - strand, within Pf1N1B4_2229 at 2540.511 kb on - strand, within Pf1N1B4_2229 at 2540.511 kb on - strand, within Pf1N1B4_2229 at 2540.511 kb on - strand, within Pf1N1B4_2229 at 2540.511 kb on - strand, within Pf1N1B4_2229 at 2540.511 kb on - strand, within Pf1N1B4_2229 at 2540.512 kb on + strand, within Pf1N1B4_2229 at 2540.512 kb on + strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.535 kb on + strand, within Pf1N1B4_2229 at 2540.535 kb on + strand, within Pf1N1B4_2229 at 2540.535 kb on + strand, within Pf1N1B4_2229 at 2540.535 kb on + strand, within Pf1N1B4_2229 at 2540.536 kb on - strand, within Pf1N1B4_2229 at 2540.536 kb on - strand, within Pf1N1B4_2229 at 2540.536 kb on - strand, within Pf1N1B4_2229 at 2540.536 kb on - strand, within Pf1N1B4_2229 at 2540.637 kb on - strand, within Pf1N1B4_2229 at 2540.672 kb on + strand, within Pf1N1B4_2229 at 2540.673 kb on - strand, within Pf1N1B4_2229 at 2540.673 kb on - strand, within Pf1N1B4_2229 at 2540.717 kb on + strand at 2540.717 kb on + strand, within Pf1N1B4_2229 at 2540.717 kb on + strand, within Pf1N1B4_2229 at 2540.789 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.855 kb on + strand, within Pf1N1B4_2229 at 2540.855 kb on + strand, within Pf1N1B4_2229 at 2540.856 kb on - strand, within Pf1N1B4_2229 at 2540.856 kb on - strand, within Pf1N1B4_2229 at 2540.856 kb on - strand, within Pf1N1B4_2229
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,538,487 - +1.4 2,538,524 + +0.8 2,538,524 + -0.4 2,538,524 + +0.7 2,538,525 - +1.0 2,538,525 - -1.2 2,538,545 + -1.8 2,538,545 + +0.4 2,538,545 + +0.1 2,538,545 + -0.4 2,538,545 + -0.4 2,538,545 + -2.9 2,538,546 - -0.9 2,538,546 - -0.2 2,538,575 + +0.5 2,538,576 - -0.6 2,538,613 + -0.7 2,538,613 + -0.1 2,538,614 - +0.1 2,538,614 - -0.4 2,538,635 - -1.2 2,538,733 + -1.4 2,538,733 + -1.4 2,538,733 + -0.2 2,538,734 - +1.2 2,538,734 - -0.1 2,538,734 - -0.6 2,538,734 - -0.6 2,538,734 - -1.2 2,538,734 - -2.5 2,538,734 - -0.1 2,538,735 + +1.7 2,538,735 + -0.5 2,538,736 - -0.6 2,539,188 - Pf1N1B4_2227 0.56 +0.2 2,539,188 - +0.4 2,539,191 - Pf1N1B4_2227 0.58 +2.0 2,539,191 - Pf1N1B4_2227 0.58 -0.2 2,539,191 - Pf1N1B4_2227 0.58 +0.4 2,539,191 - Pf1N1B4_2227 0.58 -0.4 2,539,212 - Pf1N1B4_2227 0.68 -1.2 2,539,212 - Pf1N1B4_2227 0.68 +2.1 2,539,310 - +0.9 2,539,310 - -1.4 2,539,310 - +0.4 2,539,310 - +0.7 2,539,310 - +0.7 2,539,310 - +0.9 2,539,310 - -0.2 2,539,328 - +1.1 2,539,328 - +1.6 2,539,328 - +1.0 2,539,328 - +2.4 2,539,331 - +1.8 2,539,331 - -0.9 2,539,331 - +2.4 2,539,331 - -0.9 2,539,331 - +0.8 2,539,331 - -0.9 2,539,353 + -1.8 2,539,354 - +0.1 2,539,354 - +0.1 2,539,354 - -1.0 2,539,354 - -1.0 2,539,354 - -1.6 2,539,354 - -2.5 2,539,354 - +0.1 2,539,359 - +0.4 2,539,359 - -1.2 2,539,359 - -0.6 2,539,359 - -0.3 2,539,359 - +0.1 2,539,359 - +1.6 2,539,359 - +0.1 2,539,359 - +1.1 2,539,359 - -0.2 2,539,359 - +1.9 2,539,375 - +1.4 2,539,375 - -0.8 2,539,375 - -0.6 2,539,375 - -1.6 2,539,375 - -1.2 2,539,381 - -1.4 2,539,381 - -0.4 2,539,381 - -0.6 2,539,398 - -0.4 2,539,398 - -0.9 2,539,636 + Pf1N1B4_2228 0.41 -1.0 2,539,637 - Pf1N1B4_2228 0.42 -0.2 2,539,637 - Pf1N1B4_2228 0.42 -1.8 2,539,763 + Pf1N1B4_2228 0.64 -0.5 2,539,784 - Pf1N1B4_2228 0.68 -2.2 2,539,862 + +0.4 2,539,863 - Pf1N1B4_2228 0.82 -2.3 2,539,863 - Pf1N1B4_2228 0.82 -0.8 2,539,863 - Pf1N1B4_2228 0.82 -0.3 2,539,880 + Pf1N1B4_2228 0.85 -0.6 2,539,880 + Pf1N1B4_2228 0.85 +0.8 2,539,881 - Pf1N1B4_2228 0.86 -0.8 2,539,881 - Pf1N1B4_2228 0.86 -0.2 2,539,881 - Pf1N1B4_2228 0.86 -1.4 2,539,881 - Pf1N1B4_2228 0.86 -1.4 2,539,958 + +0.4 2,539,996 + +0.7 2,540,012 + +0.4 2,540,012 + +0.5 2,540,141 + Pf1N1B4_2229 0.11 +0.2 2,540,141 + Pf1N1B4_2229 0.11 +1.1 2,540,142 - Pf1N1B4_2229 0.12 +0.8 2,540,207 + Pf1N1B4_2229 0.16 -1.4 2,540,207 + Pf1N1B4_2229 0.16 +0.7 2,540,207 + Pf1N1B4_2229 0.16 +0.4 2,540,207 + Pf1N1B4_2229 0.16 -0.3 2,540,208 - Pf1N1B4_2229 0.16 -0.4 2,540,208 - Pf1N1B4_2229 0.16 -0.4 2,540,231 - Pf1N1B4_2229 0.18 +0.6 2,540,369 + Pf1N1B4_2229 0.29 -0.9 2,540,369 + Pf1N1B4_2229 0.29 +1.1 2,540,370 - Pf1N1B4_2229 0.29 -0.6 2,540,370 - Pf1N1B4_2229 0.29 -1.8 2,540,471 + Pf1N1B4_2229 0.36 +0.5 2,540,471 + Pf1N1B4_2229 0.36 +1.4 2,540,471 + Pf1N1B4_2229 0.36 +0.6 2,540,471 + Pf1N1B4_2229 0.36 +0.6 2,540,471 + Pf1N1B4_2229 0.36 +2.4 2,540,471 + Pf1N1B4_2229 0.36 +0.2 2,540,471 + Pf1N1B4_2229 0.36 -0.6 2,540,471 + Pf1N1B4_2229 0.36 +0.5 2,540,471 + Pf1N1B4_2229 0.36 +1.4 2,540,472 - Pf1N1B4_2229 0.36 +0.4 2,540,472 - Pf1N1B4_2229 0.36 +0.6 2,540,472 - Pf1N1B4_2229 0.36 -1.4 2,540,472 - Pf1N1B4_2229 0.36 -0.1 2,540,510 + Pf1N1B4_2229 0.39 -0.6 2,540,511 - Pf1N1B4_2229 0.39 +1.0 2,540,511 - Pf1N1B4_2229 0.39 +0.4 2,540,511 - Pf1N1B4_2229 0.39 -0.4 2,540,511 - Pf1N1B4_2229 0.39 +2.0 2,540,511 - Pf1N1B4_2229 0.39 -0.8 2,540,511 - Pf1N1B4_2229 0.39 +1.4 2,540,512 + Pf1N1B4_2229 0.39 -1.1 2,540,512 + Pf1N1B4_2229 0.39 +1.0 2,540,513 - Pf1N1B4_2229 0.39 +1.1 2,540,513 - Pf1N1B4_2229 0.39 -0.9 2,540,513 - Pf1N1B4_2229 0.39 +0.9 2,540,513 - Pf1N1B4_2229 0.39 -0.5 2,540,513 - Pf1N1B4_2229 0.39 +1.4 2,540,513 - Pf1N1B4_2229 0.39 -1.8 2,540,513 - Pf1N1B4_2229 0.39 -0.7 2,540,513 - Pf1N1B4_2229 0.39 -0.9 2,540,535 + Pf1N1B4_2229 0.41 +0.0 2,540,535 + Pf1N1B4_2229 0.41 +1.1 2,540,535 + Pf1N1B4_2229 0.41 -0.9 2,540,535 + Pf1N1B4_2229 0.41 +0.3 2,540,536 - Pf1N1B4_2229 0.41 +0.4 2,540,536 - Pf1N1B4_2229 0.41 +0.6 2,540,536 - Pf1N1B4_2229 0.41 -0.2 2,540,536 - Pf1N1B4_2229 0.41 +0.7 2,540,637 - Pf1N1B4_2229 0.49 +0.6 2,540,672 + Pf1N1B4_2229 0.51 +1.2 2,540,673 - Pf1N1B4_2229 0.51 -0.9 2,540,673 - Pf1N1B4_2229 0.51 -0.9 2,540,717 + +1.4 2,540,717 + Pf1N1B4_2229 0.55 +0.7 2,540,717 + Pf1N1B4_2229 0.55 -0.4 2,540,789 + Pf1N1B4_2229 0.60 +0.3 2,540,831 + Pf1N1B4_2229 0.63 +0.9 2,540,831 + Pf1N1B4_2229 0.63 +1.0 2,540,831 + Pf1N1B4_2229 0.63 -0.8 2,540,831 + Pf1N1B4_2229 0.63 +0.8 2,540,831 + Pf1N1B4_2229 0.63 -0.6 2,540,831 + Pf1N1B4_2229 0.63 -2.5 2,540,831 + Pf1N1B4_2229 0.63 +0.3 2,540,831 + Pf1N1B4_2229 0.63 -1.1 2,540,831 + Pf1N1B4_2229 0.63 +1.7 2,540,831 + Pf1N1B4_2229 0.63 -0.9 2,540,831 + Pf1N1B4_2229 0.63 -0.7 2,540,831 + Pf1N1B4_2229 0.63 +0.4 2,540,831 + Pf1N1B4_2229 0.63 +0.1 2,540,831 + Pf1N1B4_2229 0.63 -0.6 2,540,832 - Pf1N1B4_2229 0.63 +2.1 2,540,832 - Pf1N1B4_2229 0.63 -0.6 2,540,832 - Pf1N1B4_2229 0.63 +0.1 2,540,832 - Pf1N1B4_2229 0.63 -1.4 2,540,832 - Pf1N1B4_2229 0.63 -0.9 2,540,832 - Pf1N1B4_2229 0.63 -1.4 2,540,832 - Pf1N1B4_2229 0.63 -0.6 2,540,832 - Pf1N1B4_2229 0.63 +1.4 2,540,832 - Pf1N1B4_2229 0.63 -0.0 2,540,832 - +1.4 2,540,832 - Pf1N1B4_2229 0.63 +0.4 2,540,832 - Pf1N1B4_2229 0.63 +1.6 2,540,855 + Pf1N1B4_2229 0.65 -0.1 2,540,855 + Pf1N1B4_2229 0.65 +0.4 2,540,856 - Pf1N1B4_2229 0.65 -0.2 2,540,856 - Pf1N1B4_2229 0.65 +0.4 2,540,856 - Pf1N1B4_2229 0.65 +0.4
Or see this region's nucleotide sequence