Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2222 and Pf1N1B4_2223 are separated by 139 nucleotides Pf1N1B4_2223 and Pf1N1B4_2224 are separated by 200 nucleotides Pf1N1B4_2224 and Pf1N1B4_2225 are separated by 34 nucleotides Pf1N1B4_2225 and Pf1N1B4_2226 are separated by 294 nucleotides
Pf1N1B4_2222 - Flagellar biosynthesis protein FliL, at 2,536,472 to 2,536,879
_2222
Pf1N1B4_2223 - FIG006231: RNA-binding protein, at 2,537,019 to 2,537,471
_2223
Pf1N1B4_2224 - FIG00953390: hypothetical protein, at 2,537,672 to 2,537,821
_2224
Pf1N1B4_2225 - 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2), at 2,537,856 to 2,538,461
_2225
Pf1N1B4_2226 - Z-ring-associated protein ZapA, at 2,538,756 to 2,539,073
_2226
Position (kb)
2537
2538 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2536.957 kb on - strand at 2537.020 kb on + strand at 2537.134 kb on + strand, within Pf1N1B4_2223 at 2537.135 kb on - strand, within Pf1N1B4_2223 at 2537.227 kb on + strand, within Pf1N1B4_2223 at 2537.227 kb on + strand, within Pf1N1B4_2223 at 2537.227 kb on + strand, within Pf1N1B4_2223 at 2537.228 kb on - strand, within Pf1N1B4_2223 at 2537.228 kb on - strand, within Pf1N1B4_2223 at 2537.324 kb on - strand, within Pf1N1B4_2223 at 2537.324 kb on - strand, within Pf1N1B4_2223 at 2537.324 kb on - strand, within Pf1N1B4_2223 at 2537.377 kb on + strand, within Pf1N1B4_2223 at 2537.465 kb on - strand at 2537.517 kb on + strand at 2537.517 kb on + strand at 2537.517 kb on + strand at 2537.518 kb on - strand at 2537.589 kb on + strand at 2537.589 kb on + strand at 2537.590 kb on - strand at 2537.613 kb on + strand at 2537.619 kb on + strand at 2537.619 kb on + strand at 2537.620 kb on - strand at 2537.691 kb on + strand, within Pf1N1B4_2224 at 2537.691 kb on + strand, within Pf1N1B4_2224 at 2537.691 kb on + strand, within Pf1N1B4_2224 at 2537.691 kb on + strand, within Pf1N1B4_2224 at 2537.692 kb on - strand, within Pf1N1B4_2224 at 2537.692 kb on - strand, within Pf1N1B4_2224 at 2537.708 kb on - strand, within Pf1N1B4_2224 at 2537.858 kb on - strand at 2537.869 kb on + strand at 2537.869 kb on + strand at 2537.869 kb on + strand at 2537.869 kb on + strand at 2537.869 kb on + strand at 2537.869 kb on + strand at 2537.870 kb on - strand at 2537.870 kb on - strand at 2537.870 kb on - strand at 2537.870 kb on - strand at 2537.870 kb on - strand at 2537.904 kb on + strand at 2537.904 kb on + strand at 2537.904 kb on + strand at 2537.904 kb on + strand at 2537.905 kb on - strand at 2537.905 kb on - strand at 2538.001 kb on + strand at 2538.002 kb on - strand, within Pf1N1B4_2225 at 2538.002 kb on - strand, within Pf1N1B4_2225 at 2538.019 kb on + strand, within Pf1N1B4_2225 at 2538.019 kb on + strand, within Pf1N1B4_2225 at 2538.019 kb on + strand, within Pf1N1B4_2225 at 2538.020 kb on - strand, within Pf1N1B4_2225 at 2538.020 kb on - strand, within Pf1N1B4_2225 at 2538.020 kb on - strand, within Pf1N1B4_2225 at 2538.020 kb on - strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.301 kb on + strand, within Pf1N1B4_2225 at 2538.301 kb on + strand, within Pf1N1B4_2225 at 2538.301 kb on + strand, within Pf1N1B4_2225 at 2538.302 kb on - strand, within Pf1N1B4_2225 at 2538.302 kb on - strand, within Pf1N1B4_2225 at 2538.302 kb on - strand, within Pf1N1B4_2225 at 2538.302 kb on - strand, within Pf1N1B4_2225 at 2538.353 kb on + strand, within Pf1N1B4_2225 at 2538.353 kb on + strand, within Pf1N1B4_2225 at 2538.353 kb on + strand, within Pf1N1B4_2225 at 2538.353 kb on + strand, within Pf1N1B4_2225 at 2538.354 kb on - strand, within Pf1N1B4_2225 at 2538.354 kb on - strand, within Pf1N1B4_2225 at 2538.354 kb on - strand, within Pf1N1B4_2225 at 2538.354 kb on - strand, within Pf1N1B4_2225 at 2538.354 kb on - strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.487 kb on - strand at 2538.524 kb on + strand at 2538.524 kb on + strand at 2538.524 kb on + strand at 2538.525 kb on - strand at 2538.525 kb on - strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.546 kb on - strand at 2538.546 kb on - strand at 2538.575 kb on + strand at 2538.576 kb on - strand at 2538.613 kb on + strand at 2538.613 kb on + strand at 2538.614 kb on - strand at 2538.614 kb on - strand at 2538.635 kb on - strand at 2538.733 kb on + strand at 2538.733 kb on + strand at 2538.733 kb on + strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.735 kb on + strand at 2538.735 kb on + strand at 2538.736 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,536,957 - -0.3 2,537,020 + -0.9 2,537,134 + Pf1N1B4_2223 0.25 -0.9 2,537,135 - Pf1N1B4_2223 0.26 -1.4 2,537,227 + Pf1N1B4_2223 0.46 -0.6 2,537,227 + Pf1N1B4_2223 0.46 -2.3 2,537,227 + Pf1N1B4_2223 0.46 -1.9 2,537,228 - Pf1N1B4_2223 0.46 +0.4 2,537,228 - Pf1N1B4_2223 0.46 -0.3 2,537,324 - Pf1N1B4_2223 0.67 -0.9 2,537,324 - Pf1N1B4_2223 0.67 -0.4 2,537,324 - Pf1N1B4_2223 0.67 +0.8 2,537,377 + Pf1N1B4_2223 0.79 -0.4 2,537,465 - -0.9 2,537,517 + -0.1 2,537,517 + +1.0 2,537,517 + +0.5 2,537,518 - +0.1 2,537,589 + +0.8 2,537,589 + +0.4 2,537,590 - +0.7 2,537,613 + -0.8 2,537,619 + -0.3 2,537,619 + -0.4 2,537,620 - +0.6 2,537,691 + Pf1N1B4_2224 0.13 -0.9 2,537,691 + Pf1N1B4_2224 0.13 -0.6 2,537,691 + Pf1N1B4_2224 0.13 -1.6 2,537,691 + Pf1N1B4_2224 0.13 -0.4 2,537,692 - Pf1N1B4_2224 0.13 -1.4 2,537,692 - Pf1N1B4_2224 0.13 -0.1 2,537,708 - Pf1N1B4_2224 0.24 -0.9 2,537,858 - +2.0 2,537,869 + -1.8 2,537,869 + -1.8 2,537,869 + -0.3 2,537,869 + -1.9 2,537,869 + -0.5 2,537,869 + -0.6 2,537,870 - +0.8 2,537,870 - +0.1 2,537,870 - +0.8 2,537,870 - -0.2 2,537,870 - -0.9 2,537,904 + +0.9 2,537,904 + -1.8 2,537,904 + -0.4 2,537,904 + -1.5 2,537,905 - +0.1 2,537,905 - -0.2 2,538,001 + +2.0 2,538,002 - Pf1N1B4_2225 0.24 +0.6 2,538,002 - Pf1N1B4_2225 0.24 +1.8 2,538,019 + Pf1N1B4_2225 0.27 -0.0 2,538,019 + Pf1N1B4_2225 0.27 +1.4 2,538,019 + Pf1N1B4_2225 0.27 -0.6 2,538,020 - Pf1N1B4_2225 0.27 -0.8 2,538,020 - Pf1N1B4_2225 0.27 -1.9 2,538,020 - Pf1N1B4_2225 0.27 -0.6 2,538,020 - Pf1N1B4_2225 0.27 -0.8 2,538,229 + Pf1N1B4_2225 0.62 +1.2 2,538,229 + Pf1N1B4_2225 0.62 -0.0 2,538,229 + Pf1N1B4_2225 0.62 -1.0 2,538,229 + Pf1N1B4_2225 0.62 +0.2 2,538,229 + Pf1N1B4_2225 0.62 -0.0 2,538,229 + Pf1N1B4_2225 0.62 -1.6 2,538,229 + Pf1N1B4_2225 0.62 +1.4 2,538,229 + Pf1N1B4_2225 0.62 -1.9 2,538,229 + Pf1N1B4_2225 0.62 -2.7 2,538,229 + Pf1N1B4_2225 0.62 -0.9 2,538,229 + Pf1N1B4_2225 0.62 -1.6 2,538,229 + Pf1N1B4_2225 0.62 -0.5 2,538,229 + Pf1N1B4_2225 0.62 -0.8 2,538,229 + Pf1N1B4_2225 0.62 -1.5 2,538,229 + Pf1N1B4_2225 0.62 -0.8 2,538,229 + Pf1N1B4_2225 0.62 -0.9 2,538,230 - Pf1N1B4_2225 0.62 +1.0 2,538,230 - Pf1N1B4_2225 0.62 -1.2 2,538,230 - Pf1N1B4_2225 0.62 -2.1 2,538,230 - +1.4 2,538,230 - Pf1N1B4_2225 0.62 -0.3 2,538,230 - Pf1N1B4_2225 0.62 +0.8 2,538,230 - Pf1N1B4_2225 0.62 -0.2 2,538,230 - Pf1N1B4_2225 0.62 +0.1 2,538,230 - Pf1N1B4_2225 0.62 -1.4 2,538,230 - Pf1N1B4_2225 0.62 +0.5 2,538,230 - Pf1N1B4_2225 0.62 -2.0 2,538,230 - Pf1N1B4_2225 0.62 -1.4 2,538,230 - Pf1N1B4_2225 0.62 +1.4 2,538,230 - Pf1N1B4_2225 0.62 -0.2 2,538,230 - Pf1N1B4_2225 0.62 +1.4 2,538,230 - Pf1N1B4_2225 0.62 -1.3 2,538,230 - Pf1N1B4_2225 0.62 +0.6 2,538,230 - Pf1N1B4_2225 0.62 +0.6 2,538,230 - Pf1N1B4_2225 0.62 -1.2 2,538,230 - Pf1N1B4_2225 0.62 +0.7 2,538,230 - Pf1N1B4_2225 0.62 +0.6 2,538,230 - Pf1N1B4_2225 0.62 -0.6 2,538,230 - Pf1N1B4_2225 0.62 -0.0 2,538,230 - +2.4 2,538,230 - Pf1N1B4_2225 0.62 +0.7 2,538,230 - Pf1N1B4_2225 0.62 -0.3 2,538,233 + Pf1N1B4_2225 0.62 +0.7 2,538,233 + Pf1N1B4_2225 0.62 -0.8 2,538,233 + Pf1N1B4_2225 0.62 -0.5 2,538,233 + Pf1N1B4_2225 0.62 +1.1 2,538,233 + Pf1N1B4_2225 0.62 +0.2 2,538,233 + Pf1N1B4_2225 0.62 -1.0 2,538,233 + Pf1N1B4_2225 0.62 -0.2 2,538,233 + Pf1N1B4_2225 0.62 +1.0 2,538,234 - Pf1N1B4_2225 0.62 -0.9 2,538,234 - Pf1N1B4_2225 0.62 +0.0 2,538,234 - Pf1N1B4_2225 0.62 -0.0 2,538,234 - Pf1N1B4_2225 0.62 +1.4 2,538,234 - Pf1N1B4_2225 0.62 +0.3 2,538,234 - Pf1N1B4_2225 0.62 +0.3 2,538,234 - Pf1N1B4_2225 0.62 +1.7 2,538,301 + Pf1N1B4_2225 0.73 -0.2 2,538,301 + Pf1N1B4_2225 0.73 -0.2 2,538,301 + Pf1N1B4_2225 0.73 +0.1 2,538,302 - Pf1N1B4_2225 0.74 -1.8 2,538,302 - Pf1N1B4_2225 0.74 +0.2 2,538,302 - Pf1N1B4_2225 0.74 +2.0 2,538,302 - Pf1N1B4_2225 0.74 -0.4 2,538,353 + Pf1N1B4_2225 0.82 -0.1 2,538,353 + Pf1N1B4_2225 0.82 -0.9 2,538,353 + Pf1N1B4_2225 0.82 -1.4 2,538,353 + Pf1N1B4_2225 0.82 +1.1 2,538,354 - Pf1N1B4_2225 0.82 -1.8 2,538,354 - Pf1N1B4_2225 0.82 +1.6 2,538,354 - Pf1N1B4_2225 0.82 +0.8 2,538,354 - Pf1N1B4_2225 0.82 +0.4 2,538,354 - Pf1N1B4_2225 0.82 +0.1 2,538,355 + Pf1N1B4_2225 0.82 -0.4 2,538,355 + Pf1N1B4_2225 0.82 -0.6 2,538,355 + Pf1N1B4_2225 0.82 +0.2 2,538,355 + Pf1N1B4_2225 0.82 -0.3 2,538,355 + Pf1N1B4_2225 0.82 -0.6 2,538,355 + Pf1N1B4_2225 0.82 +0.1 2,538,355 + Pf1N1B4_2225 0.82 -0.7 2,538,355 + Pf1N1B4_2225 0.82 -0.3 2,538,355 + Pf1N1B4_2225 0.82 -1.9 2,538,355 + Pf1N1B4_2225 0.82 +0.4 2,538,356 - Pf1N1B4_2225 0.83 -0.4 2,538,356 - Pf1N1B4_2225 0.83 +0.7 2,538,356 - Pf1N1B4_2225 0.83 +0.4 2,538,356 - Pf1N1B4_2225 0.83 +0.4 2,538,356 - Pf1N1B4_2225 0.83 -0.6 2,538,356 - Pf1N1B4_2225 0.83 -0.3 2,538,356 - Pf1N1B4_2225 0.83 -0.1 2,538,487 - +1.4 2,538,524 + +0.8 2,538,524 + -0.4 2,538,524 + +0.7 2,538,525 - +1.0 2,538,525 - -1.2 2,538,545 + -1.8 2,538,545 + +0.4 2,538,545 + +0.1 2,538,545 + -0.4 2,538,545 + -0.4 2,538,545 + -2.9 2,538,546 - -0.9 2,538,546 - -0.2 2,538,575 + +0.5 2,538,576 - -0.6 2,538,613 + -0.7 2,538,613 + -0.1 2,538,614 - +0.1 2,538,614 - -0.4 2,538,635 - -1.2 2,538,733 + -1.4 2,538,733 + -1.4 2,538,733 + -0.2 2,538,734 - +1.2 2,538,734 - -0.1 2,538,734 - -0.6 2,538,734 - -0.6 2,538,734 - -1.2 2,538,734 - -2.5 2,538,734 - -0.1 2,538,735 + +1.7 2,538,735 + -0.5 2,538,736 - -0.6
Or see this region's nucleotide sequence