Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2060 and Pf1N1B4_2061 are separated by 577 nucleotides Pf1N1B4_2061 and Pf1N1B4_2062 are separated by 82 nucleotides Pf1N1B4_2062 and Pf1N1B4_2063 are separated by 233 nucleotides
Pf1N1B4_2059 - Uncharacterized protein ImpF, at 2,352,132 to 2,352,644
_2059
Pf1N1B4_2060 - hypothetical protein, at 2,352,638 to 2,353,063
_2060
Pf1N1B4_2061 - hypothetical protein, at 2,353,641 to 2,354,078
_2061
Pf1N1B4_2062 - Uncharacterized protein ImpD, at 2,354,161 to 2,354,649
_2062
Pf1N1B4_2063 - Uncharacterized protein ImpC, at 2,354,883 to 2,356,382
_2063
Position (kb)
2353
2354
2355 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2352.646 kb on + strand at 2352.646 kb on + strand at 2352.647 kb on - strand at 2352.647 kb on - strand at 2352.660 kb on + strand at 2352.660 kb on + strand at 2352.660 kb on + strand at 2352.660 kb on + strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.703 kb on + strand, within Pf1N1B4_2060 at 2352.743 kb on - strand, within Pf1N1B4_2060 at 2352.854 kb on - strand, within Pf1N1B4_2060 at 2353.210 kb on + strand at 2353.211 kb on - strand at 2353.218 kb on + strand at 2353.320 kb on + strand at 2353.396 kb on + strand at 2353.430 kb on + strand at 2353.431 kb on - strand at 2353.431 kb on - strand at 2353.507 kb on + strand at 2353.508 kb on - strand at 2353.512 kb on + strand at 2353.512 kb on + strand at 2353.512 kb on + strand at 2353.512 kb on + strand at 2353.513 kb on - strand at 2353.513 kb on - strand at 2353.640 kb on - strand at 2353.719 kb on + strand, within Pf1N1B4_2061 at 2353.719 kb on + strand, within Pf1N1B4_2061 at 2353.770 kb on - strand, within Pf1N1B4_2061 at 2353.770 kb on - strand, within Pf1N1B4_2061 at 2353.933 kb on + strand, within Pf1N1B4_2061 at 2353.933 kb on + strand, within Pf1N1B4_2061 at 2353.934 kb on - strand, within Pf1N1B4_2061 at 2353.934 kb on - strand, within Pf1N1B4_2061 at 2353.985 kb on + strand, within Pf1N1B4_2061 at 2353.986 kb on - strand, within Pf1N1B4_2061 at 2354.057 kb on + strand at 2354.141 kb on - strand at 2354.360 kb on + strand, within Pf1N1B4_2062 at 2354.360 kb on + strand, within Pf1N1B4_2062 at 2354.360 kb on + strand, within Pf1N1B4_2062 at 2354.361 kb on - strand, within Pf1N1B4_2062 at 2354.361 kb on - strand, within Pf1N1B4_2062 at 2354.361 kb on - strand, within Pf1N1B4_2062 at 2354.361 kb on - strand, within Pf1N1B4_2062 at 2354.361 kb on - strand, within Pf1N1B4_2062 at 2354.361 kb on - strand, within Pf1N1B4_2062 at 2354.361 kb on - strand, within Pf1N1B4_2062 at 2354.361 kb on - strand, within Pf1N1B4_2062 at 2354.468 kb on + strand, within Pf1N1B4_2062 at 2354.468 kb on + strand, within Pf1N1B4_2062 at 2354.468 kb on + strand, within Pf1N1B4_2062 at 2354.468 kb on + strand, within Pf1N1B4_2062 at 2354.468 kb on + strand, within Pf1N1B4_2062 at 2354.468 kb on + strand, within Pf1N1B4_2062 at 2354.469 kb on - strand, within Pf1N1B4_2062 at 2354.469 kb on - strand, within Pf1N1B4_2062 at 2354.469 kb on - strand, within Pf1N1B4_2062 at 2354.637 kb on + strand at 2354.672 kb on - strand at 2354.884 kb on + strand at 2354.885 kb on - strand at 2354.950 kb on + strand at 2354.965 kb on + strand at 2354.965 kb on + strand at 2354.966 kb on - strand at 2354.966 kb on - strand at 2354.966 kb on - strand at 2354.980 kb on + strand at 2354.980 kb on + strand at 2354.980 kb on + strand at 2354.980 kb on + strand at 2354.980 kb on + strand at 2354.980 kb on + strand at 2354.980 kb on + strand at 2354.981 kb on - strand at 2354.981 kb on - strand at 2354.981 kb on - strand at 2354.981 kb on - strand at 2354.981 kb on - strand at 2354.984 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,352,646 + +0.5 2,352,646 + -0.7 2,352,647 - +0.5 2,352,647 - +0.5 2,352,660 + -0.8 2,352,660 + -1.3 2,352,660 + +0.1 2,352,660 + +0.3 2,352,661 - -1.3 2,352,661 - +0.3 2,352,661 - +2.5 2,352,661 - +1.0 2,352,661 - +0.7 2,352,661 - -1.5 2,352,661 - +1.1 2,352,661 - -0.8 2,352,703 + Pf1N1B4_2060 0.15 -0.7 2,352,743 - Pf1N1B4_2060 0.25 -0.3 2,352,854 - Pf1N1B4_2060 0.51 -1.3 2,353,210 + -0.5 2,353,211 - -0.3 2,353,218 + +2.2 2,353,320 + +0.5 2,353,396 + +0.5 2,353,430 + +0.2 2,353,431 - +1.1 2,353,431 - -1.7 2,353,507 + +0.1 2,353,508 - -0.6 2,353,512 + +0.9 2,353,512 + -0.4 2,353,512 + -0.2 2,353,512 + +1.3 2,353,513 - -0.0 2,353,513 - -1.2 2,353,640 - +1.5 2,353,719 + Pf1N1B4_2061 0.18 -0.8 2,353,719 + Pf1N1B4_2061 0.18 -1.8 2,353,770 - Pf1N1B4_2061 0.29 -0.3 2,353,770 - Pf1N1B4_2061 0.29 -0.8 2,353,933 + Pf1N1B4_2061 0.67 +0.9 2,353,933 + Pf1N1B4_2061 0.67 -0.5 2,353,934 - Pf1N1B4_2061 0.67 -0.6 2,353,934 - Pf1N1B4_2061 0.67 +0.5 2,353,985 + Pf1N1B4_2061 0.79 +0.5 2,353,986 - Pf1N1B4_2061 0.79 -0.8 2,354,057 + -1.0 2,354,141 - +1.1 2,354,360 + Pf1N1B4_2062 0.41 +0.4 2,354,360 + Pf1N1B4_2062 0.41 -1.1 2,354,360 + Pf1N1B4_2062 0.41 -0.3 2,354,361 - Pf1N1B4_2062 0.41 -0.5 2,354,361 - Pf1N1B4_2062 0.41 +0.2 2,354,361 - Pf1N1B4_2062 0.41 +0.3 2,354,361 - Pf1N1B4_2062 0.41 +0.3 2,354,361 - Pf1N1B4_2062 0.41 +0.3 2,354,361 - Pf1N1B4_2062 0.41 +0.2 2,354,361 - Pf1N1B4_2062 0.41 +0.7 2,354,361 - Pf1N1B4_2062 0.41 +0.1 2,354,468 + Pf1N1B4_2062 0.63 -1.1 2,354,468 + Pf1N1B4_2062 0.63 -0.2 2,354,468 + Pf1N1B4_2062 0.63 +0.1 2,354,468 + Pf1N1B4_2062 0.63 -0.9 2,354,468 + Pf1N1B4_2062 0.63 +0.1 2,354,468 + Pf1N1B4_2062 0.63 +2.2 2,354,469 - Pf1N1B4_2062 0.63 -1.1 2,354,469 - Pf1N1B4_2062 0.63 +0.5 2,354,469 - Pf1N1B4_2062 0.63 -2.8 2,354,637 + -0.3 2,354,672 - -1.5 2,354,884 + -0.2 2,354,885 - +0.3 2,354,950 + +0.9 2,354,965 + -1.7 2,354,965 + -1.8 2,354,966 - -0.8 2,354,966 - -1.1 2,354,966 - +0.5 2,354,980 + +1.5 2,354,980 + -0.1 2,354,980 + -0.3 2,354,980 + -1.3 2,354,980 + +0.5 2,354,980 + +0.6 2,354,980 + -0.3 2,354,981 - +0.7 2,354,981 - +0.9 2,354,981 - +0.5 2,354,981 - +2.1 2,354,981 - -1.3 2,354,984 - +0.9
Or see this region's nucleotide sequence