Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1920 and Pf1N1B4_1921 are separated by 10 nucleotides Pf1N1B4_1921 and Pf1N1B4_1922 are separated by 81 nucleotides Pf1N1B4_1922 and Pf1N1B4_1923 are separated by 181 nucleotides
Pf1N1B4_1920 - Glycyl-tRNA synthetase beta chain (EC 6.1.1.14), at 2,194,032 to 2,196,086
_1920
Pf1N1B4_1921 - D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase (EC 3.1.1.-); Histidinol-phosphatase (EC 3.1.3.15), at 2,196,097 to 2,196,630
_1921
Pf1N1B4_1922 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 2,196,712 to 2,197,491
_1922
Pf1N1B4_1923 - Signal transduction histidine kinase, at 2,197,673 to 2,199,985
_1923
Position (kb)
2196
2197
2198 Strain fitness (log2 ratio)
-2
-1
0
1 at 2197.563 kb on + strand at 2197.582 kb on + strand at 2197.603 kb on + strand at 2197.603 kb on + strand at 2197.603 kb on + strand at 2197.604 kb on - strand at 2197.635 kb on + strand at 2197.638 kb on - strand at 2197.674 kb on + strand at 2197.685 kb on + strand at 2197.692 kb on - strand at 2197.692 kb on - strand at 2197.819 kb on + strand at 2197.898 kb on + strand at 2197.899 kb on - strand at 2197.914 kb on + strand, within Pf1N1B4_1923 at 2197.914 kb on + strand, within Pf1N1B4_1923 at 2197.914 kb on + strand, within Pf1N1B4_1923 at 2197.914 kb on + strand, within Pf1N1B4_1923 at 2197.914 kb on + strand, within Pf1N1B4_1923 at 2197.914 kb on + strand, within Pf1N1B4_1923 at 2197.915 kb on - strand, within Pf1N1B4_1923 at 2197.915 kb on - strand, within Pf1N1B4_1923 at 2197.915 kb on - strand, within Pf1N1B4_1923 at 2197.915 kb on - strand, within Pf1N1B4_1923 at 2197.915 kb on - strand, within Pf1N1B4_1923 at 2197.915 kb on - strand, within Pf1N1B4_1923 at 2197.915 kb on - strand, within Pf1N1B4_1923 at 2197.953 kb on + strand, within Pf1N1B4_1923 at 2197.953 kb on + strand, within Pf1N1B4_1923 at 2197.953 kb on + strand, within Pf1N1B4_1923 at 2197.953 kb on + strand, within Pf1N1B4_1923 at 2197.953 kb on + strand, within Pf1N1B4_1923 at 2197.953 kb on + strand, within Pf1N1B4_1923 at 2197.954 kb on - strand, within Pf1N1B4_1923 at 2197.954 kb on - strand, within Pf1N1B4_1923 at 2197.954 kb on - strand, within Pf1N1B4_1923 at 2197.954 kb on - strand, within Pf1N1B4_1923 at 2197.954 kb on - strand, within Pf1N1B4_1923 at 2197.954 kb on - strand, within Pf1N1B4_1923 at 2197.954 kb on - strand, within Pf1N1B4_1923 at 2198.099 kb on - strand, within Pf1N1B4_1923 at 2198.140 kb on - strand, within Pf1N1B4_1923 at 2198.140 kb on - strand, within Pf1N1B4_1923 at 2198.140 kb on - strand, within Pf1N1B4_1923 at 2198.201 kb on + strand, within Pf1N1B4_1923 at 2198.201 kb on + strand, within Pf1N1B4_1923 at 2198.201 kb on + strand, within Pf1N1B4_1923 at 2198.201 kb on + strand, within Pf1N1B4_1923 at 2198.201 kb on + strand, within Pf1N1B4_1923 at 2198.202 kb on - strand, within Pf1N1B4_1923 at 2198.202 kb on - strand, within Pf1N1B4_1923 at 2198.202 kb on - strand, within Pf1N1B4_1923 at 2198.202 kb on - strand, within Pf1N1B4_1923 at 2198.202 kb on - strand, within Pf1N1B4_1923 at 2198.202 kb on - strand, within Pf1N1B4_1923 at 2198.202 kb on - strand, within Pf1N1B4_1923 at 2198.202 kb on - strand, within Pf1N1B4_1923 at 2198.202 kb on - strand, within Pf1N1B4_1923 at 2198.207 kb on + strand, within Pf1N1B4_1923 at 2198.207 kb on + strand, within Pf1N1B4_1923 at 2198.207 kb on + strand, within Pf1N1B4_1923 at 2198.207 kb on + strand, within Pf1N1B4_1923 at 2198.207 kb on + strand, within Pf1N1B4_1923 at 2198.207 kb on + strand, within Pf1N1B4_1923 at 2198.208 kb on - strand, within Pf1N1B4_1923 at 2198.208 kb on - strand, within Pf1N1B4_1923 at 2198.208 kb on - strand, within Pf1N1B4_1923 at 2198.208 kb on - strand, within Pf1N1B4_1923 at 2198.208 kb on - strand, within Pf1N1B4_1923 at 2198.208 kb on - strand, within Pf1N1B4_1923 at 2198.375 kb on + strand, within Pf1N1B4_1923 at 2198.375 kb on + strand, within Pf1N1B4_1923 at 2198.375 kb on + strand, within Pf1N1B4_1923 at 2198.375 kb on + strand, within Pf1N1B4_1923 at 2198.375 kb on + strand, within Pf1N1B4_1923 at 2198.375 kb on + strand, within Pf1N1B4_1923 at 2198.375 kb on + strand, within Pf1N1B4_1923 at 2198.376 kb on - strand, within Pf1N1B4_1923 at 2198.376 kb on - strand, within Pf1N1B4_1923 at 2198.376 kb on - strand, within Pf1N1B4_1923 at 2198.376 kb on - strand, within Pf1N1B4_1923 at 2198.376 kb on - strand, within Pf1N1B4_1923 at 2198.376 kb on - strand, within Pf1N1B4_1923 at 2198.376 kb on - strand, within Pf1N1B4_1923 at 2198.378 kb on + strand, within Pf1N1B4_1923 at 2198.379 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,197,563 + -0.3 2,197,582 + -0.2 2,197,603 + +0.2 2,197,603 + +0.1 2,197,603 + +1.0 2,197,604 - +0.5 2,197,635 + +1.6 2,197,638 - -0.1 2,197,674 + +0.2 2,197,685 + -1.1 2,197,692 - +1.1 2,197,692 - -0.2 2,197,819 + +0.9 2,197,898 + +0.6 2,197,899 - -1.8 2,197,914 + Pf1N1B4_1923 0.10 +0.2 2,197,914 + Pf1N1B4_1923 0.10 -2.1 2,197,914 + Pf1N1B4_1923 0.10 -0.2 2,197,914 + Pf1N1B4_1923 0.10 -0.2 2,197,914 + Pf1N1B4_1923 0.10 +0.5 2,197,914 + Pf1N1B4_1923 0.10 +0.8 2,197,915 - Pf1N1B4_1923 0.10 +1.1 2,197,915 - Pf1N1B4_1923 0.10 +1.7 2,197,915 - Pf1N1B4_1923 0.10 +0.5 2,197,915 - Pf1N1B4_1923 0.10 -0.6 2,197,915 - Pf1N1B4_1923 0.10 -0.3 2,197,915 - Pf1N1B4_1923 0.10 -1.3 2,197,915 - Pf1N1B4_1923 0.10 -0.2 2,197,953 + Pf1N1B4_1923 0.12 -0.0 2,197,953 + Pf1N1B4_1923 0.12 -0.8 2,197,953 + Pf1N1B4_1923 0.12 -0.4 2,197,953 + Pf1N1B4_1923 0.12 -0.8 2,197,953 + Pf1N1B4_1923 0.12 +0.2 2,197,953 + Pf1N1B4_1923 0.12 +1.2 2,197,954 - Pf1N1B4_1923 0.12 -0.9 2,197,954 - Pf1N1B4_1923 0.12 -0.9 2,197,954 - Pf1N1B4_1923 0.12 -0.8 2,197,954 - Pf1N1B4_1923 0.12 -0.5 2,197,954 - Pf1N1B4_1923 0.12 +0.5 2,197,954 - Pf1N1B4_1923 0.12 -0.9 2,197,954 - Pf1N1B4_1923 0.12 +1.6 2,198,099 - Pf1N1B4_1923 0.18 +0.0 2,198,140 - Pf1N1B4_1923 0.20 +0.5 2,198,140 - Pf1N1B4_1923 0.20 -0.5 2,198,140 - Pf1N1B4_1923 0.20 -1.3 2,198,201 + Pf1N1B4_1923 0.23 -1.3 2,198,201 + Pf1N1B4_1923 0.23 -2.3 2,198,201 + Pf1N1B4_1923 0.23 -1.3 2,198,201 + Pf1N1B4_1923 0.23 -0.8 2,198,201 + Pf1N1B4_1923 0.23 -0.5 2,198,202 - Pf1N1B4_1923 0.23 -0.2 2,198,202 - Pf1N1B4_1923 0.23 +0.9 2,198,202 - Pf1N1B4_1923 0.23 +0.8 2,198,202 - Pf1N1B4_1923 0.23 +0.7 2,198,202 - Pf1N1B4_1923 0.23 -0.2 2,198,202 - Pf1N1B4_1923 0.23 +1.1 2,198,202 - Pf1N1B4_1923 0.23 -1.1 2,198,202 - Pf1N1B4_1923 0.23 +0.2 2,198,202 - Pf1N1B4_1923 0.23 -1.0 2,198,207 + Pf1N1B4_1923 0.23 +0.5 2,198,207 + Pf1N1B4_1923 0.23 +0.5 2,198,207 + Pf1N1B4_1923 0.23 -0.8 2,198,207 + Pf1N1B4_1923 0.23 -1.1 2,198,207 + Pf1N1B4_1923 0.23 +0.4 2,198,207 + Pf1N1B4_1923 0.23 -0.8 2,198,208 - Pf1N1B4_1923 0.23 +0.1 2,198,208 - Pf1N1B4_1923 0.23 -2.3 2,198,208 - Pf1N1B4_1923 0.23 -0.5 2,198,208 - Pf1N1B4_1923 0.23 -0.8 2,198,208 - Pf1N1B4_1923 0.23 +0.8 2,198,208 - Pf1N1B4_1923 0.23 +0.8 2,198,375 + Pf1N1B4_1923 0.30 -1.7 2,198,375 + Pf1N1B4_1923 0.30 +0.4 2,198,375 + Pf1N1B4_1923 0.30 +1.1 2,198,375 + Pf1N1B4_1923 0.30 +0.2 2,198,375 + Pf1N1B4_1923 0.30 -1.1 2,198,375 + Pf1N1B4_1923 0.30 -0.1 2,198,375 + Pf1N1B4_1923 0.30 +0.5 2,198,376 - Pf1N1B4_1923 0.30 -0.5 2,198,376 - Pf1N1B4_1923 0.30 -1.7 2,198,376 - Pf1N1B4_1923 0.30 +0.5 2,198,376 - Pf1N1B4_1923 0.30 -0.1 2,198,376 - Pf1N1B4_1923 0.30 +0.8 2,198,376 - Pf1N1B4_1923 0.30 -0.1 2,198,376 - Pf1N1B4_1923 0.30 -2.6 2,198,378 + Pf1N1B4_1923 0.30 -0.3 2,198,379 - +1.5
Or see this region's nucleotide sequence