Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1888 and Pf1N1B4_1889 are separated by 204 nucleotides Pf1N1B4_1889 and Pf1N1B4_1890 are separated by 119 nucleotides Pf1N1B4_1890 and Pf1N1B4_1891 overlap by 4 nucleotides
Pf1N1B4_1888 - Glutamate-aspartate carrier protein, at 2,160,086 to 2,161,417
_1888
Pf1N1B4_1889 - Fused spore maturation proteins A and B, at 2,161,622 to 2,162,851
_1889
Pf1N1B4_1890 - Membrane lipoprotein lipid attachment site containing protein USSDB6D, at 2,162,971 to 2,163,597
_1890
Pf1N1B4_1891 - ABC-type transport system involved in resistance to organic solvents, periplasmic component USSDB6C, at 2,163,594 to 2,164,532
_1891
Position (kb)
2161
2162
2163 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2160.698 kb on + strand, within Pf1N1B4_1888 at 2160.698 kb on + strand at 2160.699 kb on - strand, within Pf1N1B4_1888 at 2160.794 kb on + strand, within Pf1N1B4_1888 at 2160.794 kb on + strand, within Pf1N1B4_1888 at 2160.794 kb on + strand, within Pf1N1B4_1888 at 2160.794 kb on + strand, within Pf1N1B4_1888 at 2160.794 kb on + strand, within Pf1N1B4_1888 at 2160.794 kb on + strand, within Pf1N1B4_1888 at 2160.795 kb on - strand, within Pf1N1B4_1888 at 2160.795 kb on - strand, within Pf1N1B4_1888 at 2160.898 kb on - strand at 2161.038 kb on - strand, within Pf1N1B4_1888 at 2161.074 kb on - strand, within Pf1N1B4_1888 at 2161.321 kb on + strand at 2161.321 kb on + strand at 2161.321 kb on + strand at 2161.321 kb on + strand at 2161.322 kb on - strand at 2161.322 kb on - strand at 2161.322 kb on - strand at 2161.322 kb on - strand at 2161.322 kb on - strand at 2161.322 kb on - strand at 2161.325 kb on + strand at 2161.325 kb on + strand at 2161.325 kb on + strand at 2161.326 kb on - strand at 2161.326 kb on - strand at 2161.326 kb on - strand at 2161.326 kb on - strand at 2161.326 kb on - strand at 2161.536 kb on + strand at 2161.536 kb on + strand at 2161.539 kb on + strand at 2161.540 kb on - strand at 2161.540 kb on - strand at 2161.581 kb on - strand at 2161.589 kb on - strand at 2161.627 kb on + strand at 2161.628 kb on - strand at 2161.628 kb on - strand at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.055 kb on + strand, within Pf1N1B4_1889 at 2162.055 kb on + strand, within Pf1N1B4_1889 at 2162.055 kb on + strand, within Pf1N1B4_1889 at 2162.056 kb on - strand, within Pf1N1B4_1889 at 2162.056 kb on - strand, within Pf1N1B4_1889 at 2162.056 kb on - strand, within Pf1N1B4_1889 at 2162.056 kb on - strand, within Pf1N1B4_1889 at 2162.056 kb on - strand, within Pf1N1B4_1889 at 2162.056 kb on - strand, within Pf1N1B4_1889 at 2162.099 kb on + strand, within Pf1N1B4_1889 at 2162.099 kb on + strand, within Pf1N1B4_1889 at 2162.099 kb on + strand, within Pf1N1B4_1889 at 2162.099 kb on + strand, within Pf1N1B4_1889 at 2162.099 kb on + strand, within Pf1N1B4_1889 at 2162.100 kb on - strand, within Pf1N1B4_1889 at 2162.100 kb on - strand, within Pf1N1B4_1889 at 2162.240 kb on + strand, within Pf1N1B4_1889 at 2162.240 kb on + strand, within Pf1N1B4_1889 at 2162.241 kb on - strand, within Pf1N1B4_1889 at 2162.241 kb on - strand, within Pf1N1B4_1889 at 2162.241 kb on - strand, within Pf1N1B4_1889 at 2162.241 kb on - strand, within Pf1N1B4_1889 at 2162.385 kb on + strand, within Pf1N1B4_1889 at 2162.385 kb on + strand, within Pf1N1B4_1889 at 2162.386 kb on - strand, within Pf1N1B4_1889 at 2162.386 kb on - strand, within Pf1N1B4_1889 at 2162.402 kb on + strand, within Pf1N1B4_1889 at 2162.402 kb on + strand, within Pf1N1B4_1889 at 2162.403 kb on - strand, within Pf1N1B4_1889 at 2162.403 kb on - strand, within Pf1N1B4_1889 at 2162.462 kb on + strand, within Pf1N1B4_1889 at 2162.462 kb on + strand, within Pf1N1B4_1889 at 2162.463 kb on - strand, within Pf1N1B4_1889 at 2162.664 kb on + strand, within Pf1N1B4_1889 at 2162.665 kb on - strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.727 kb on + strand, within Pf1N1B4_1889 at 2162.727 kb on + strand, within Pf1N1B4_1889 at 2162.727 kb on + strand, within Pf1N1B4_1889 at 2162.727 kb on + strand, within Pf1N1B4_1889 at 2162.728 kb on - strand, within Pf1N1B4_1889 at 2162.728 kb on - strand at 2162.728 kb on - strand, within Pf1N1B4_1889 at 2162.728 kb on - strand, within Pf1N1B4_1889 at 2162.728 kb on - strand, within Pf1N1B4_1889 at 2162.738 kb on + strand at 2162.738 kb on + strand at 2162.738 kb on + strand at 2162.738 kb on + strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.834 kb on + strand at 2162.918 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.954 kb on - strand at 2162.954 kb on - strand at 2162.954 kb on - strand at 2162.972 kb on + strand at 2162.973 kb on - strand at 2162.973 kb on - strand at 2162.973 kb on - strand at 2163.201 kb on - strand, within Pf1N1B4_1890 at 2163.255 kb on + strand, within Pf1N1B4_1890 at 2163.255 kb on + strand, within Pf1N1B4_1890 at 2163.255 kb on + strand, within Pf1N1B4_1890 at 2163.256 kb on - strand, within Pf1N1B4_1890 at 2163.256 kb on - strand, within Pf1N1B4_1890 at 2163.256 kb on - strand, within Pf1N1B4_1890 at 2163.362 kb on + strand, within Pf1N1B4_1890 at 2163.362 kb on + strand, within Pf1N1B4_1890 at 2163.363 kb on - strand, within Pf1N1B4_1890 at 2163.363 kb on - strand, within Pf1N1B4_1890 at 2163.363 kb on - strand, within Pf1N1B4_1890 at 2163.509 kb on + strand, within Pf1N1B4_1890 at 2163.510 kb on - strand, within Pf1N1B4_1890 at 2163.634 kb on + strand at 2163.791 kb on + strand, within Pf1N1B4_1891 at 2163.792 kb on - strand, within Pf1N1B4_1891
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,160,698 + Pf1N1B4_1888 0.46 +0.7 2,160,698 + +2.5 2,160,699 - Pf1N1B4_1888 0.46 +0.8 2,160,794 + Pf1N1B4_1888 0.53 +0.5 2,160,794 + Pf1N1B4_1888 0.53 -0.5 2,160,794 + Pf1N1B4_1888 0.53 -1.5 2,160,794 + Pf1N1B4_1888 0.53 +0.8 2,160,794 + Pf1N1B4_1888 0.53 +0.9 2,160,794 + Pf1N1B4_1888 0.53 -0.8 2,160,795 - Pf1N1B4_1888 0.53 -0.8 2,160,795 - Pf1N1B4_1888 0.53 -1.1 2,160,898 - -0.1 2,161,038 - Pf1N1B4_1888 0.71 +0.0 2,161,074 - Pf1N1B4_1888 0.74 -0.1 2,161,321 + -0.5 2,161,321 + -0.8 2,161,321 + +1.5 2,161,321 + -0.5 2,161,322 - +1.1 2,161,322 - +1.5 2,161,322 - +0.2 2,161,322 - -0.2 2,161,322 - +0.5 2,161,322 - -0.1 2,161,325 + -1.8 2,161,325 + +1.1 2,161,325 + +1.2 2,161,326 - -0.2 2,161,326 - +0.8 2,161,326 - -1.2 2,161,326 - +1.9 2,161,326 - -0.4 2,161,536 + +0.2 2,161,536 + +1.2 2,161,539 + -0.4 2,161,540 - +0.9 2,161,540 - +0.2 2,161,581 - -2.3 2,161,589 - +0.4 2,161,627 + +0.2 2,161,628 - +0.3 2,161,628 - +0.4 2,162,004 + Pf1N1B4_1889 0.31 +1.9 2,162,004 + Pf1N1B4_1889 0.31 +1.4 2,162,004 + Pf1N1B4_1889 0.31 +1.6 2,162,004 + Pf1N1B4_1889 0.31 -0.1 2,162,004 + Pf1N1B4_1889 0.31 +0.3 2,162,004 + Pf1N1B4_1889 0.31 +0.8 2,162,004 + Pf1N1B4_1889 0.31 +1.3 2,162,004 + Pf1N1B4_1889 0.31 +0.8 2,162,004 + Pf1N1B4_1889 0.31 +1.0 2,162,005 - Pf1N1B4_1889 0.31 +0.8 2,162,005 - Pf1N1B4_1889 0.31 -0.5 2,162,005 - Pf1N1B4_1889 0.31 +0.3 2,162,005 - Pf1N1B4_1889 0.31 +2.5 2,162,005 - Pf1N1B4_1889 0.31 +1.3 2,162,005 - Pf1N1B4_1889 0.31 +0.1 2,162,005 - Pf1N1B4_1889 0.31 +1.9 2,162,005 - Pf1N1B4_1889 0.31 +0.7 2,162,055 + Pf1N1B4_1889 0.35 +0.5 2,162,055 + Pf1N1B4_1889 0.35 +0.4 2,162,055 + Pf1N1B4_1889 0.35 +0.7 2,162,056 - Pf1N1B4_1889 0.35 +0.3 2,162,056 - Pf1N1B4_1889 0.35 +0.5 2,162,056 - Pf1N1B4_1889 0.35 +0.4 2,162,056 - Pf1N1B4_1889 0.35 +0.5 2,162,056 - Pf1N1B4_1889 0.35 +0.3 2,162,056 - Pf1N1B4_1889 0.35 -0.0 2,162,099 + Pf1N1B4_1889 0.39 +0.3 2,162,099 + Pf1N1B4_1889 0.39 +1.2 2,162,099 + Pf1N1B4_1889 0.39 +1.1 2,162,099 + Pf1N1B4_1889 0.39 +0.7 2,162,099 + Pf1N1B4_1889 0.39 -0.8 2,162,100 - Pf1N1B4_1889 0.39 +1.1 2,162,100 - Pf1N1B4_1889 0.39 -0.1 2,162,240 + Pf1N1B4_1889 0.50 -0.2 2,162,240 + Pf1N1B4_1889 0.50 +0.6 2,162,241 - Pf1N1B4_1889 0.50 +0.7 2,162,241 - Pf1N1B4_1889 0.50 +1.1 2,162,241 - Pf1N1B4_1889 0.50 +0.2 2,162,241 - Pf1N1B4_1889 0.50 -1.2 2,162,385 + Pf1N1B4_1889 0.62 +1.8 2,162,385 + Pf1N1B4_1889 0.62 -1.7 2,162,386 - Pf1N1B4_1889 0.62 +1.2 2,162,386 - Pf1N1B4_1889 0.62 +0.4 2,162,402 + Pf1N1B4_1889 0.63 +0.5 2,162,402 + Pf1N1B4_1889 0.63 +0.6 2,162,403 - Pf1N1B4_1889 0.63 +1.4 2,162,403 - Pf1N1B4_1889 0.63 +1.4 2,162,462 + Pf1N1B4_1889 0.68 +0.8 2,162,462 + Pf1N1B4_1889 0.68 +0.8 2,162,463 - Pf1N1B4_1889 0.68 +1.0 2,162,664 + Pf1N1B4_1889 0.85 +0.4 2,162,665 - Pf1N1B4_1889 0.85 +0.8 2,162,723 + Pf1N1B4_1889 0.90 +1.2 2,162,723 + Pf1N1B4_1889 0.90 +0.3 2,162,723 + Pf1N1B4_1889 0.90 +0.9 2,162,723 + Pf1N1B4_1889 0.90 -2.3 2,162,723 + Pf1N1B4_1889 0.90 +0.4 2,162,723 + Pf1N1B4_1889 0.90 +1.1 2,162,723 + Pf1N1B4_1889 0.90 -0.1 2,162,723 + Pf1N1B4_1889 0.90 +1.1 2,162,723 + Pf1N1B4_1889 0.90 +0.5 2,162,723 + Pf1N1B4_1889 0.90 +0.0 2,162,723 + Pf1N1B4_1889 0.90 +1.2 2,162,723 + Pf1N1B4_1889 0.90 +0.3 2,162,723 + Pf1N1B4_1889 0.90 +0.1 2,162,723 + Pf1N1B4_1889 0.90 +0.8 2,162,723 + Pf1N1B4_1889 0.90 +1.3 2,162,723 + Pf1N1B4_1889 0.90 +1.0 2,162,723 + Pf1N1B4_1889 0.90 -0.2 2,162,723 + Pf1N1B4_1889 0.90 +0.8 2,162,723 + Pf1N1B4_1889 0.90 +0.6 2,162,723 + Pf1N1B4_1889 0.90 +0.7 2,162,723 + Pf1N1B4_1889 0.90 +1.2 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 +0.1 2,162,723 + Pf1N1B4_1889 0.90 +0.6 2,162,724 - Pf1N1B4_1889 0.90 +0.6 2,162,724 - Pf1N1B4_1889 0.90 +1.5 2,162,724 - Pf1N1B4_1889 0.90 +1.0 2,162,724 - Pf1N1B4_1889 0.90 +1.4 2,162,724 - Pf1N1B4_1889 0.90 +1.8 2,162,724 - Pf1N1B4_1889 0.90 +0.5 2,162,724 - Pf1N1B4_1889 0.90 +0.7 2,162,724 - Pf1N1B4_1889 0.90 +0.5 2,162,724 - Pf1N1B4_1889 0.90 +1.2 2,162,724 - Pf1N1B4_1889 0.90 +1.9 2,162,724 - Pf1N1B4_1889 0.90 +1.8 2,162,724 - Pf1N1B4_1889 0.90 +0.5 2,162,724 - Pf1N1B4_1889 0.90 +0.7 2,162,724 - Pf1N1B4_1889 0.90 -1.0 2,162,724 - Pf1N1B4_1889 0.90 +1.2 2,162,724 - Pf1N1B4_1889 0.90 +0.3 2,162,724 - Pf1N1B4_1889 0.90 -0.4 2,162,724 - Pf1N1B4_1889 0.90 +1.6 2,162,724 - Pf1N1B4_1889 0.90 -0.5 2,162,724 - Pf1N1B4_1889 0.90 +1.4 2,162,724 - Pf1N1B4_1889 0.90 -0.1 2,162,724 - Pf1N1B4_1889 0.90 +0.2 2,162,724 - Pf1N1B4_1889 0.90 +0.9 2,162,724 - Pf1N1B4_1889 0.90 +1.8 2,162,724 - Pf1N1B4_1889 0.90 +0.8 2,162,724 - Pf1N1B4_1889 0.90 +1.8 2,162,724 - Pf1N1B4_1889 0.90 +0.9 2,162,724 - Pf1N1B4_1889 0.90 +0.4 2,162,724 - Pf1N1B4_1889 0.90 -1.0 2,162,727 + Pf1N1B4_1889 0.90 +1.1 2,162,727 + Pf1N1B4_1889 0.90 +0.6 2,162,727 + Pf1N1B4_1889 0.90 +1.0 2,162,727 + Pf1N1B4_1889 0.90 +0.4 2,162,728 - Pf1N1B4_1889 0.90 +0.7 2,162,728 - +1.5 2,162,728 - Pf1N1B4_1889 0.90 +0.5 2,162,728 - Pf1N1B4_1889 0.90 +0.9 2,162,728 - Pf1N1B4_1889 0.90 +0.6 2,162,738 + +0.0 2,162,738 + +0.3 2,162,738 + +1.5 2,162,738 + -0.0 2,162,739 - +1.3 2,162,739 - +1.5 2,162,739 - +0.5 2,162,739 - +1.5 2,162,739 - +0.7 2,162,739 - -0.5 2,162,739 - +0.1 2,162,739 - +1.3 2,162,739 - +0.1 2,162,739 - +0.8 2,162,834 + -0.8 2,162,918 + -0.1 2,162,953 + -0.9 2,162,953 + +0.3 2,162,953 + +0.8 2,162,953 + -0.1 2,162,953 + -0.8 2,162,953 + -0.7 2,162,953 + +1.2 2,162,953 + -0.9 2,162,953 + +0.5 2,162,953 + -0.2 2,162,953 + -0.5 2,162,953 + -0.2 2,162,953 + -0.4 2,162,953 + +0.5 2,162,953 + +1.2 2,162,953 + +0.8 2,162,953 + +1.5 2,162,954 - -0.1 2,162,954 - -1.8 2,162,954 - -1.1 2,162,972 + +1.5 2,162,973 - -1.3 2,162,973 - +0.0 2,162,973 - -0.1 2,163,201 - Pf1N1B4_1890 0.37 +1.0 2,163,255 + Pf1N1B4_1890 0.45 -0.7 2,163,255 + Pf1N1B4_1890 0.45 -1.7 2,163,255 + Pf1N1B4_1890 0.45 -2.2 2,163,256 - Pf1N1B4_1890 0.45 -0.5 2,163,256 - Pf1N1B4_1890 0.45 -2.3 2,163,256 - Pf1N1B4_1890 0.45 +0.5 2,163,362 + Pf1N1B4_1890 0.62 +0.2 2,163,362 + Pf1N1B4_1890 0.62 +1.1 2,163,363 - Pf1N1B4_1890 0.63 -0.1 2,163,363 - Pf1N1B4_1890 0.63 -0.1 2,163,363 - Pf1N1B4_1890 0.63 -0.6 2,163,509 + Pf1N1B4_1890 0.86 +0.5 2,163,510 - Pf1N1B4_1890 0.86 -0.1 2,163,634 + -0.5 2,163,791 + Pf1N1B4_1891 0.21 -1.1 2,163,792 - Pf1N1B4_1891 0.21 -0.4
Or see this region's nucleotide sequence