Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1845 and Pf1N1B4_1846 are separated by 42 nucleotides Pf1N1B4_1846 and Pf1N1B4_1847 are separated by 408 nucleotides Pf1N1B4_1847 and Pf1N1B4_1848 are separated by 311 nucleotides
Pf1N1B4_1845 - FIG00954395: hypothetical protein, at 2,118,272 to 2,118,559
_1845
Pf1N1B4_1846 - Protein tyrosine/serine phosphatase, at 2,118,602 to 2,119,093
_1846
Pf1N1B4_1847 - hypothetical protein, at 2,119,502 to 2,119,726
_1847
Pf1N1B4_1848 - Radical SAM domain protein, at 2,120,038 to 2,121,096
_1848
Position (kb)
2119
2120 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2118.720 kb on + strand, within Pf1N1B4_1846 at 2118.720 kb on + strand, within Pf1N1B4_1846 at 2118.720 kb on + strand, within Pf1N1B4_1846 at 2118.721 kb on - strand, within Pf1N1B4_1846 at 2118.793 kb on + strand, within Pf1N1B4_1846 at 2118.820 kb on - strand, within Pf1N1B4_1846 at 2118.868 kb on + strand, within Pf1N1B4_1846 at 2118.869 kb on - strand, within Pf1N1B4_1846 at 2118.869 kb on - strand, within Pf1N1B4_1846 at 2118.920 kb on + strand, within Pf1N1B4_1846 at 2118.920 kb on + strand, within Pf1N1B4_1846 at 2118.920 kb on + strand, within Pf1N1B4_1846 at 2118.920 kb on + strand, within Pf1N1B4_1846 at 2118.921 kb on - strand, within Pf1N1B4_1846 at 2118.921 kb on - strand, within Pf1N1B4_1846 at 2118.921 kb on - strand, within Pf1N1B4_1846 at 2118.921 kb on - strand, within Pf1N1B4_1846 at 2118.921 kb on - strand, within Pf1N1B4_1846 at 2118.921 kb on - strand, within Pf1N1B4_1846 at 2118.921 kb on - strand, within Pf1N1B4_1846 at 2118.921 kb on - strand, within Pf1N1B4_1846 at 2118.921 kb on - strand, within Pf1N1B4_1846 at 2118.921 kb on - strand, within Pf1N1B4_1846 at 2118.962 kb on + strand, within Pf1N1B4_1846 at 2118.962 kb on + strand, within Pf1N1B4_1846 at 2118.962 kb on + strand, within Pf1N1B4_1846 at 2118.962 kb on + strand, within Pf1N1B4_1846 at 2118.962 kb on + strand, within Pf1N1B4_1846 at 2118.963 kb on - strand, within Pf1N1B4_1846 at 2118.963 kb on - strand, within Pf1N1B4_1846 at 2118.963 kb on - strand, within Pf1N1B4_1846 at 2118.963 kb on - strand, within Pf1N1B4_1846 at 2118.963 kb on - strand, within Pf1N1B4_1846 at 2118.963 kb on - strand, within Pf1N1B4_1846 at 2118.963 kb on - strand, within Pf1N1B4_1846 at 2118.963 kb on - strand, within Pf1N1B4_1846 at 2118.963 kb on - strand, within Pf1N1B4_1846 at 2118.963 kb on - strand, within Pf1N1B4_1846 at 2118.963 kb on - strand, within Pf1N1B4_1846 at 2118.963 kb on - strand, within Pf1N1B4_1846 at 2119.022 kb on + strand, within Pf1N1B4_1846 at 2119.022 kb on + strand, within Pf1N1B4_1846 at 2119.022 kb on + strand, within Pf1N1B4_1846 at 2119.226 kb on + strand at 2119.227 kb on - strand at 2119.227 kb on - strand at 2119.227 kb on - strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.228 kb on + strand at 2119.229 kb on - strand at 2119.229 kb on - strand at 2119.229 kb on - strand at 2119.229 kb on - strand at 2119.229 kb on - strand at 2119.229 kb on - strand at 2119.229 kb on - strand at 2119.229 kb on - strand at 2119.229 kb on - strand at 2119.230 kb on + strand at 2119.230 kb on + strand at 2119.230 kb on + strand at 2119.231 kb on - strand at 2119.397 kb on + strand at 2119.398 kb on - strand at 2119.496 kb on - strand at 2119.511 kb on + strand at 2119.512 kb on - strand at 2119.524 kb on + strand at 2119.525 kb on - strand, within Pf1N1B4_1847 at 2119.525 kb on - strand, within Pf1N1B4_1847 at 2119.607 kb on + strand, within Pf1N1B4_1847 at 2119.608 kb on - strand, within Pf1N1B4_1847 at 2119.608 kb on - strand, within Pf1N1B4_1847 at 2119.608 kb on - strand, within Pf1N1B4_1847 at 2119.608 kb on - strand, within Pf1N1B4_1847 at 2119.608 kb on - strand, within Pf1N1B4_1847 at 2119.608 kb on - strand, within Pf1N1B4_1847 at 2119.646 kb on + strand at 2119.646 kb on + strand, within Pf1N1B4_1847 at 2119.646 kb on + strand, within Pf1N1B4_1847 at 2119.646 kb on + strand, within Pf1N1B4_1847 at 2119.646 kb on + strand, within Pf1N1B4_1847 at 2119.647 kb on - strand, within Pf1N1B4_1847 at 2119.647 kb on - strand, within Pf1N1B4_1847 at 2119.647 kb on - strand, within Pf1N1B4_1847 at 2119.647 kb on - strand, within Pf1N1B4_1847 at 2119.647 kb on - strand, within Pf1N1B4_1847 at 2119.647 kb on - strand, within Pf1N1B4_1847 at 2119.651 kb on - strand, within Pf1N1B4_1847 at 2119.679 kb on + strand, within Pf1N1B4_1847 at 2119.679 kb on + strand, within Pf1N1B4_1847 at 2119.680 kb on - strand, within Pf1N1B4_1847 at 2119.770 kb on - strand at 2119.822 kb on + strand at 2119.823 kb on - strand at 2119.860 kb on + strand at 2119.870 kb on - strand at 2119.893 kb on - strand at 2119.922 kb on + strand at 2119.933 kb on + strand at 2119.933 kb on + strand at 2119.937 kb on - strand at 2120.009 kb on + strand at 2120.010 kb on - strand at 2120.018 kb on - strand at 2120.033 kb on - strand at 2120.033 kb on - strand at 2120.135 kb on - strand at 2120.205 kb on + strand, within Pf1N1B4_1848 at 2120.205 kb on + strand, within Pf1N1B4_1848 at 2120.205 kb on + strand, within Pf1N1B4_1848 at 2120.206 kb on - strand, within Pf1N1B4_1848 at 2120.206 kb on - strand, within Pf1N1B4_1848 at 2120.206 kb on - strand, within Pf1N1B4_1848 at 2120.206 kb on - strand, within Pf1N1B4_1848 at 2120.272 kb on + strand, within Pf1N1B4_1848 at 2120.272 kb on + strand, within Pf1N1B4_1848 at 2120.272 kb on + strand, within Pf1N1B4_1848 at 2120.272 kb on + strand, within Pf1N1B4_1848 at 2120.272 kb on + strand, within Pf1N1B4_1848 at 2120.272 kb on + strand, within Pf1N1B4_1848 at 2120.272 kb on + strand, within Pf1N1B4_1848 at 2120.272 kb on + strand, within Pf1N1B4_1848 at 2120.272 kb on + strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.273 kb on - strand, within Pf1N1B4_1848 at 2120.278 kb on + strand, within Pf1N1B4_1848 at 2120.278 kb on + strand, within Pf1N1B4_1848 at 2120.278 kb on + strand, within Pf1N1B4_1848 at 2120.278 kb on + strand, within Pf1N1B4_1848 at 2120.279 kb on - strand, within Pf1N1B4_1848 at 2120.279 kb on - strand, within Pf1N1B4_1848 at 2120.279 kb on - strand, within Pf1N1B4_1848 at 2120.300 kb on - strand, within Pf1N1B4_1848 at 2120.437 kb on + strand, within Pf1N1B4_1848 at 2120.472 kb on + strand, within Pf1N1B4_1848 at 2120.472 kb on + strand, within Pf1N1B4_1848 at 2120.472 kb on + strand, within Pf1N1B4_1848 at 2120.524 kb on - strand, within Pf1N1B4_1848 at 2120.524 kb on - strand, within Pf1N1B4_1848 at 2120.591 kb on + strand, within Pf1N1B4_1848 at 2120.632 kb on - strand, within Pf1N1B4_1848
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,118,720 + Pf1N1B4_1846 0.24 -0.7 2,118,720 + Pf1N1B4_1846 0.24 -1.1 2,118,720 + Pf1N1B4_1846 0.24 +1.5 2,118,721 - Pf1N1B4_1846 0.24 -0.8 2,118,793 + Pf1N1B4_1846 0.39 +0.2 2,118,820 - Pf1N1B4_1846 0.44 -1.6 2,118,868 + Pf1N1B4_1846 0.54 +1.5 2,118,869 - Pf1N1B4_1846 0.54 -1.5 2,118,869 - Pf1N1B4_1846 0.54 -0.5 2,118,920 + Pf1N1B4_1846 0.65 +0.1 2,118,920 + Pf1N1B4_1846 0.65 -0.3 2,118,920 + Pf1N1B4_1846 0.65 +0.2 2,118,920 + Pf1N1B4_1846 0.65 +1.4 2,118,921 - Pf1N1B4_1846 0.65 -1.9 2,118,921 - Pf1N1B4_1846 0.65 +0.1 2,118,921 - Pf1N1B4_1846 0.65 -0.8 2,118,921 - Pf1N1B4_1846 0.65 -0.5 2,118,921 - Pf1N1B4_1846 0.65 +0.5 2,118,921 - Pf1N1B4_1846 0.65 -0.6 2,118,921 - Pf1N1B4_1846 0.65 -0.2 2,118,921 - Pf1N1B4_1846 0.65 -1.2 2,118,921 - Pf1N1B4_1846 0.65 -1.5 2,118,921 - Pf1N1B4_1846 0.65 +0.5 2,118,962 + Pf1N1B4_1846 0.73 -0.4 2,118,962 + Pf1N1B4_1846 0.73 +1.5 2,118,962 + Pf1N1B4_1846 0.73 -0.8 2,118,962 + Pf1N1B4_1846 0.73 +1.4 2,118,962 + Pf1N1B4_1846 0.73 +0.7 2,118,963 - Pf1N1B4_1846 0.73 +1.1 2,118,963 - Pf1N1B4_1846 0.73 +1.7 2,118,963 - Pf1N1B4_1846 0.73 -2.3 2,118,963 - Pf1N1B4_1846 0.73 +0.7 2,118,963 - Pf1N1B4_1846 0.73 +0.8 2,118,963 - Pf1N1B4_1846 0.73 -0.4 2,118,963 - Pf1N1B4_1846 0.73 +0.3 2,118,963 - Pf1N1B4_1846 0.73 +0.2 2,118,963 - Pf1N1B4_1846 0.73 -0.9 2,118,963 - Pf1N1B4_1846 0.73 +1.0 2,118,963 - Pf1N1B4_1846 0.73 -0.5 2,118,963 - Pf1N1B4_1846 0.73 +0.5 2,119,022 + Pf1N1B4_1846 0.85 -1.5 2,119,022 + Pf1N1B4_1846 0.85 -0.4 2,119,022 + Pf1N1B4_1846 0.85 -0.8 2,119,226 + +1.2 2,119,227 - -0.8 2,119,227 - -0.8 2,119,227 - +0.3 2,119,228 + +1.5 2,119,228 + +0.5 2,119,228 + +0.9 2,119,228 + -0.5 2,119,228 + -0.5 2,119,228 + +0.4 2,119,228 + -0.7 2,119,228 + +0.9 2,119,228 + -0.8 2,119,228 + +0.5 2,119,228 + +0.5 2,119,228 + -1.7 2,119,228 + -0.1 2,119,228 + -1.3 2,119,228 + +0.9 2,119,228 + -0.5 2,119,228 + +0.5 2,119,228 + +1.3 2,119,228 + +0.5 2,119,229 - -0.1 2,119,229 - +0.7 2,119,229 - +0.6 2,119,229 - -0.3 2,119,229 - -0.9 2,119,229 - -0.8 2,119,229 - -1.4 2,119,229 - +0.2 2,119,229 - +1.0 2,119,230 + +0.1 2,119,230 + -0.3 2,119,230 + +2.2 2,119,231 - +0.3 2,119,397 + +2.1 2,119,398 - -0.1 2,119,496 - +0.1 2,119,511 + +1.5 2,119,512 - +0.1 2,119,524 + +0.6 2,119,525 - Pf1N1B4_1847 0.10 +0.1 2,119,525 - Pf1N1B4_1847 0.10 +0.8 2,119,607 + Pf1N1B4_1847 0.47 +1.5 2,119,608 - Pf1N1B4_1847 0.47 +1.5 2,119,608 - Pf1N1B4_1847 0.47 +0.5 2,119,608 - Pf1N1B4_1847 0.47 +0.5 2,119,608 - Pf1N1B4_1847 0.47 +1.7 2,119,608 - Pf1N1B4_1847 0.47 +0.1 2,119,608 - Pf1N1B4_1847 0.47 -1.5 2,119,646 + +1.5 2,119,646 + Pf1N1B4_1847 0.64 +0.5 2,119,646 + Pf1N1B4_1847 0.64 +1.1 2,119,646 + Pf1N1B4_1847 0.64 -1.9 2,119,646 + Pf1N1B4_1847 0.64 -0.7 2,119,647 - Pf1N1B4_1847 0.64 +1.2 2,119,647 - Pf1N1B4_1847 0.64 -0.9 2,119,647 - Pf1N1B4_1847 0.64 +1.3 2,119,647 - Pf1N1B4_1847 0.64 -0.9 2,119,647 - Pf1N1B4_1847 0.64 +0.4 2,119,647 - Pf1N1B4_1847 0.64 +1.7 2,119,651 - Pf1N1B4_1847 0.66 -0.1 2,119,679 + Pf1N1B4_1847 0.79 -1.5 2,119,679 + Pf1N1B4_1847 0.79 -1.3 2,119,680 - Pf1N1B4_1847 0.79 -0.3 2,119,770 - +0.9 2,119,822 + +0.0 2,119,823 - +0.9 2,119,860 + -1.1 2,119,870 - +0.8 2,119,893 - +1.4 2,119,922 + -0.5 2,119,933 + +1.9 2,119,933 + +1.5 2,119,937 - -0.5 2,120,009 + +0.5 2,120,010 - -2.1 2,120,018 - -0.4 2,120,033 - +0.5 2,120,033 - +1.5 2,120,135 - -1.0 2,120,205 + Pf1N1B4_1848 0.16 +0.5 2,120,205 + Pf1N1B4_1848 0.16 +1.1 2,120,205 + Pf1N1B4_1848 0.16 +0.9 2,120,206 - Pf1N1B4_1848 0.16 +1.0 2,120,206 - Pf1N1B4_1848 0.16 -0.9 2,120,206 - Pf1N1B4_1848 0.16 +0.3 2,120,206 - Pf1N1B4_1848 0.16 -0.1 2,120,272 + Pf1N1B4_1848 0.22 -0.1 2,120,272 + Pf1N1B4_1848 0.22 +1.1 2,120,272 + Pf1N1B4_1848 0.22 +0.8 2,120,272 + Pf1N1B4_1848 0.22 +1.2 2,120,272 + Pf1N1B4_1848 0.22 -0.4 2,120,272 + Pf1N1B4_1848 0.22 -1.3 2,120,272 + Pf1N1B4_1848 0.22 +0.9 2,120,272 + Pf1N1B4_1848 0.22 +1.0 2,120,272 + Pf1N1B4_1848 0.22 +1.5 2,120,273 - Pf1N1B4_1848 0.22 -0.5 2,120,273 - Pf1N1B4_1848 0.22 +1.2 2,120,273 - Pf1N1B4_1848 0.22 +0.1 2,120,273 - Pf1N1B4_1848 0.22 +0.4 2,120,273 - Pf1N1B4_1848 0.22 -0.3 2,120,273 - Pf1N1B4_1848 0.22 -0.1 2,120,273 - Pf1N1B4_1848 0.22 +0.3 2,120,273 - Pf1N1B4_1848 0.22 +0.6 2,120,273 - Pf1N1B4_1848 0.22 -2.9 2,120,273 - Pf1N1B4_1848 0.22 -0.1 2,120,273 - Pf1N1B4_1848 0.22 -0.2 2,120,273 - Pf1N1B4_1848 0.22 +0.1 2,120,273 - Pf1N1B4_1848 0.22 -1.1 2,120,273 - Pf1N1B4_1848 0.22 +2.1 2,120,273 - Pf1N1B4_1848 0.22 -0.0 2,120,273 - Pf1N1B4_1848 0.22 -1.3 2,120,273 - Pf1N1B4_1848 0.22 +1.5 2,120,273 - Pf1N1B4_1848 0.22 +0.5 2,120,273 - Pf1N1B4_1848 0.22 -1.1 2,120,273 - Pf1N1B4_1848 0.22 +0.2 2,120,273 - Pf1N1B4_1848 0.22 -0.0 2,120,273 - Pf1N1B4_1848 0.22 +1.0 2,120,273 - Pf1N1B4_1848 0.22 -0.1 2,120,273 - Pf1N1B4_1848 0.22 -0.6 2,120,273 - Pf1N1B4_1848 0.22 +0.4 2,120,273 - Pf1N1B4_1848 0.22 +1.1 2,120,273 - +0.5 2,120,273 - Pf1N1B4_1848 0.22 -1.0 2,120,273 - Pf1N1B4_1848 0.22 +2.1 2,120,273 - Pf1N1B4_1848 0.22 -0.3 2,120,273 - Pf1N1B4_1848 0.22 -1.0 2,120,273 - Pf1N1B4_1848 0.22 -0.5 2,120,278 + Pf1N1B4_1848 0.23 +0.4 2,120,278 + Pf1N1B4_1848 0.23 -0.9 2,120,278 + Pf1N1B4_1848 0.23 +1.5 2,120,278 + Pf1N1B4_1848 0.23 -0.9 2,120,279 - Pf1N1B4_1848 0.23 -0.4 2,120,279 - Pf1N1B4_1848 0.23 -1.0 2,120,279 - Pf1N1B4_1848 0.23 +0.9 2,120,300 - Pf1N1B4_1848 0.25 -0.3 2,120,437 + Pf1N1B4_1848 0.38 -1.1 2,120,472 + Pf1N1B4_1848 0.41 -0.9 2,120,472 + Pf1N1B4_1848 0.41 +1.2 2,120,472 + Pf1N1B4_1848 0.41 +0.1 2,120,524 - Pf1N1B4_1848 0.46 +0.6 2,120,524 - Pf1N1B4_1848 0.46 -1.1 2,120,591 + Pf1N1B4_1848 0.52 -0.4 2,120,632 - Pf1N1B4_1848 0.56 +0.2
Or see this region's nucleotide sequence