Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_183 and Pf1N1B4_184 are separated by 157 nucleotides Pf1N1B4_184 and Pf1N1B4_185 are separated by 11 nucleotides
Pf1N1B4_183 - Major facilitator family transporter, at 225,982 to 227,283
_183
Pf1N1B4_184 - Chromosome initiation inhibitor, at 227,441 to 228,400
_184
Pf1N1B4_185 - Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129), at 228,412 to 230,304
_185
Position (kb)
227
228
229 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 226.494 kb on - strand, within Pf1N1B4_183 at 226.505 kb on + strand, within Pf1N1B4_183 at 226.505 kb on + strand, within Pf1N1B4_183 at 226.506 kb on - strand, within Pf1N1B4_183 at 226.506 kb on - strand, within Pf1N1B4_183 at 226.839 kb on - strand, within Pf1N1B4_183 at 226.839 kb on - strand at 226.960 kb on - strand, within Pf1N1B4_183 at 227.201 kb on + strand at 227.201 kb on + strand at 227.202 kb on - strand at 227.202 kb on - strand at 227.202 kb on - strand at 227.208 kb on - strand at 227.217 kb on - strand at 227.275 kb on - strand at 227.298 kb on + strand at 227.298 kb on + strand at 227.298 kb on + strand at 227.298 kb on + strand at 227.298 kb on + strand at 227.299 kb on - strand at 227.299 kb on - strand at 227.299 kb on - strand at 227.299 kb on - strand at 227.299 kb on - strand at 227.302 kb on + strand at 227.302 kb on + strand at 227.302 kb on + strand at 227.303 kb on - strand at 227.305 kb on - strand at 227.318 kb on + strand at 227.358 kb on + strand at 227.358 kb on + strand at 227.359 kb on - strand at 227.359 kb on - strand at 227.364 kb on + strand at 227.409 kb on + strand at 227.412 kb on + strand at 227.427 kb on + strand at 227.440 kb on + strand at 227.456 kb on - strand at 227.516 kb on + strand at 227.516 kb on + strand at 227.517 kb on - strand at 227.517 kb on - strand at 227.807 kb on + strand, within Pf1N1B4_184 at 227.967 kb on + strand, within Pf1N1B4_184 at 227.984 kb on + strand, within Pf1N1B4_184 at 227.984 kb on + strand, within Pf1N1B4_184 at 227.984 kb on + strand, within Pf1N1B4_184 at 227.984 kb on + strand, within Pf1N1B4_184 at 227.985 kb on - strand, within Pf1N1B4_184 at 227.991 kb on - strand, within Pf1N1B4_184 at 228.179 kb on + strand, within Pf1N1B4_184 at 228.179 kb on + strand, within Pf1N1B4_184 at 228.231 kb on + strand, within Pf1N1B4_184 at 228.306 kb on + strand at 228.306 kb on + strand at 228.306 kb on + strand at 228.306 kb on + strand at 228.307 kb on - strand at 228.379 kb on + strand at 228.414 kb on - strand at 228.452 kb on + strand at 228.453 kb on - strand at 228.647 kb on + strand, within Pf1N1B4_185 at 228.648 kb on - strand, within Pf1N1B4_185 at 228.757 kb on + strand, within Pf1N1B4_185 at 228.757 kb on + strand, within Pf1N1B4_185 at 228.758 kb on - strand, within Pf1N1B4_185 at 228.808 kb on + strand, within Pf1N1B4_185 at 228.809 kb on - strand, within Pf1N1B4_185 at 228.945 kb on - strand, within Pf1N1B4_185 at 228.945 kb on - strand, within Pf1N1B4_185 at 228.945 kb on - strand, within Pf1N1B4_185 at 229.094 kb on + strand, within Pf1N1B4_185 at 229.094 kb on + strand, within Pf1N1B4_185 at 229.094 kb on + strand, within Pf1N1B4_185 at 229.094 kb on + strand, within Pf1N1B4_185 at 229.095 kb on - strand, within Pf1N1B4_185 at 229.137 kb on - strand, within Pf1N1B4_185 at 229.137 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.214 kb on + strand at 229.214 kb on + strand, within Pf1N1B4_185 at 229.218 kb on + strand, within Pf1N1B4_185 at 229.218 kb on + strand, within Pf1N1B4_185 at 229.218 kb on + strand, within Pf1N1B4_185 at 229.219 kb on - strand, within Pf1N1B4_185 at 229.219 kb on - strand, within Pf1N1B4_185 at 229.219 kb on - strand, within Pf1N1B4_185 at 229.219 kb on - strand, within Pf1N1B4_185 at 229.219 kb on - strand, within Pf1N1B4_185 at 229.241 kb on + strand, within Pf1N1B4_185 at 229.398 kb on - strand, within Pf1N1B4_185 at 229.398 kb on - strand, within Pf1N1B4_185
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 226,494 - Pf1N1B4_183 0.39 -0.3 226,505 + Pf1N1B4_183 0.40 +1.2 226,505 + Pf1N1B4_183 0.40 -0.8 226,506 - Pf1N1B4_183 0.40 -0.1 226,506 - Pf1N1B4_183 0.40 -1.8 226,839 - Pf1N1B4_183 0.66 +1.1 226,839 - +0.3 226,960 - Pf1N1B4_183 0.75 +0.2 227,201 + +0.7 227,201 + -0.3 227,202 - +0.2 227,202 - +0.0 227,202 - +0.7 227,208 - +1.7 227,217 - -1.6 227,275 - -1.3 227,298 + +0.4 227,298 + +0.0 227,298 + -0.3 227,298 + +0.3 227,298 + -1.6 227,299 - +1.1 227,299 - +0.7 227,299 - +0.8 227,299 - -0.5 227,299 - -0.8 227,302 + +0.0 227,302 + -0.1 227,302 + -0.3 227,303 - +0.2 227,305 - +1.4 227,318 + -1.1 227,358 + -0.5 227,358 + -0.6 227,359 - +1.7 227,359 - -0.6 227,364 + -0.6 227,409 + -0.5 227,412 + +1.3 227,427 + -0.3 227,440 + -0.6 227,456 - -0.8 227,516 + -0.2 227,516 + +0.7 227,517 - -0.6 227,517 - +0.7 227,807 + Pf1N1B4_184 0.38 -1.0 227,967 + Pf1N1B4_184 0.55 -1.6 227,984 + Pf1N1B4_184 0.57 -0.3 227,984 + Pf1N1B4_184 0.57 -1.6 227,984 + Pf1N1B4_184 0.57 -2.1 227,984 + Pf1N1B4_184 0.57 -0.8 227,985 - Pf1N1B4_184 0.57 -0.1 227,991 - Pf1N1B4_184 0.57 -2.8 228,179 + Pf1N1B4_184 0.77 -1.6 228,179 + Pf1N1B4_184 0.77 -2.1 228,231 + Pf1N1B4_184 0.82 -2.1 228,306 + -0.8 228,306 + -2.1 228,306 + -0.3 228,306 + -1.6 228,307 - -2.1 228,379 + -0.1 228,414 - -0.6 228,452 + +1.3 228,453 - -1.1 228,647 + Pf1N1B4_185 0.12 -1.0 228,648 - Pf1N1B4_185 0.12 -0.1 228,757 + Pf1N1B4_185 0.18 -2.2 228,757 + Pf1N1B4_185 0.18 +0.7 228,758 - Pf1N1B4_185 0.18 +2.3 228,808 + Pf1N1B4_185 0.21 -0.1 228,809 - Pf1N1B4_185 0.21 -0.8 228,945 - Pf1N1B4_185 0.28 -1.1 228,945 - Pf1N1B4_185 0.28 -0.3 228,945 - Pf1N1B4_185 0.28 +0.2 229,094 + Pf1N1B4_185 0.36 -2.1 229,094 + Pf1N1B4_185 0.36 -2.6 229,094 + Pf1N1B4_185 0.36 +0.1 229,094 + Pf1N1B4_185 0.36 +1.5 229,095 - Pf1N1B4_185 0.36 -0.4 229,137 - Pf1N1B4_185 0.38 -1.1 229,137 - Pf1N1B4_185 0.38 -0.3 229,143 - Pf1N1B4_185 0.39 +0.1 229,143 - Pf1N1B4_185 0.39 +0.7 229,143 - Pf1N1B4_185 0.39 +4.1 229,143 - Pf1N1B4_185 0.39 +0.1 229,143 - Pf1N1B4_185 0.39 +1.1 229,143 - Pf1N1B4_185 0.39 -1.3 229,143 - Pf1N1B4_185 0.39 -1.4 229,143 - Pf1N1B4_185 0.39 +0.2 229,143 - Pf1N1B4_185 0.39 +0.7 229,143 - Pf1N1B4_185 0.39 -0.8 229,214 + +0.7 229,214 + Pf1N1B4_185 0.42 -0.8 229,218 + Pf1N1B4_185 0.43 +0.7 229,218 + Pf1N1B4_185 0.43 +0.5 229,218 + Pf1N1B4_185 0.43 +1.5 229,219 - Pf1N1B4_185 0.43 +0.7 229,219 - Pf1N1B4_185 0.43 +0.2 229,219 - Pf1N1B4_185 0.43 -1.0 229,219 - Pf1N1B4_185 0.43 +0.0 229,219 - Pf1N1B4_185 0.43 -0.3 229,241 + Pf1N1B4_185 0.44 -0.8 229,398 - Pf1N1B4_185 0.52 -1.3 229,398 - Pf1N1B4_185 0.52 +0.6
Or see this region's nucleotide sequence