Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1681 and Pf1N1B4_1682 are separated by 12 nucleotides Pf1N1B4_1682 and Pf1N1B4_1683 are separated by 72 nucleotides Pf1N1B4_1683 and Pf1N1B4_1684 are separated by 123 nucleotides
Pf1N1B4_1681 - Peptidase M50, at 1,927,320 to 1,929,416
_1681
Pf1N1B4_1682 - Acetyltransferase, GNAT family (EC 2.3.1.-), at 1,929,429 to 1,929,926
_1682
Pf1N1B4_1683 - Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17), at 1,929,999 to 1,930,841
_1683
Pf1N1B4_1684 - Oxidoreductase, aldo/keto reductase family, at 1,930,965 to 1,932,002
_1684
Position (kb)
1929
1930
1931 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1929.051 kb on + strand, within Pf1N1B4_1681 at 1929.174 kb on + strand, within Pf1N1B4_1681 at 1929.174 kb on + strand, within Pf1N1B4_1681 at 1929.174 kb on + strand, within Pf1N1B4_1681 at 1929.174 kb on + strand, within Pf1N1B4_1681 at 1929.174 kb on + strand, within Pf1N1B4_1681 at 1929.174 kb on + strand at 1929.175 kb on - strand, within Pf1N1B4_1681 at 1929.175 kb on - strand, within Pf1N1B4_1681 at 1929.175 kb on - strand, within Pf1N1B4_1681 at 1929.175 kb on - strand at 1929.334 kb on + strand at 1929.574 kb on + strand, within Pf1N1B4_1682 at 1929.574 kb on + strand, within Pf1N1B4_1682 at 1929.574 kb on + strand at 1929.574 kb on + strand, within Pf1N1B4_1682 at 1929.574 kb on + strand, within Pf1N1B4_1682 at 1929.574 kb on + strand, within Pf1N1B4_1682 at 1929.574 kb on + strand, within Pf1N1B4_1682 at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.575 kb on - strand at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.639 kb on + strand at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.640 kb on - strand, within Pf1N1B4_1682 at 1929.640 kb on - strand, within Pf1N1B4_1682 at 1929.714 kb on + strand, within Pf1N1B4_1682 at 1929.714 kb on + strand, within Pf1N1B4_1682 at 1929.714 kb on + strand, within Pf1N1B4_1682 at 1929.715 kb on - strand, within Pf1N1B4_1682 at 1929.715 kb on - strand, within Pf1N1B4_1682 at 1929.776 kb on - strand, within Pf1N1B4_1682 at 1929.852 kb on + strand, within Pf1N1B4_1682 at 1929.852 kb on + strand, within Pf1N1B4_1682 at 1929.852 kb on + strand, within Pf1N1B4_1682 at 1929.853 kb on - strand, within Pf1N1B4_1682 at 1929.913 kb on - strand at 1930.004 kb on + strand at 1930.182 kb on + strand, within Pf1N1B4_1683 at 1930.388 kb on + strand, within Pf1N1B4_1683 at 1930.389 kb on - strand, within Pf1N1B4_1683 at 1930.493 kb on + strand, within Pf1N1B4_1683 at 1930.967 kb on - strand at 1930.967 kb on - strand at 1931.298 kb on + strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.302 kb on + strand, within Pf1N1B4_1684 at 1931.302 kb on + strand, within Pf1N1B4_1684 at 1931.302 kb on + strand, within Pf1N1B4_1684 at 1931.302 kb on + strand, within Pf1N1B4_1684 at 1931.302 kb on + strand, within Pf1N1B4_1684 at 1931.303 kb on - strand, within Pf1N1B4_1684 at 1931.413 kb on + strand, within Pf1N1B4_1684 at 1931.414 kb on - strand, within Pf1N1B4_1684 at 1931.414 kb on - strand, within Pf1N1B4_1684 at 1931.414 kb on - strand, within Pf1N1B4_1684 at 1931.440 kb on - strand at 1931.440 kb on - strand, within Pf1N1B4_1684 at 1931.440 kb on - strand, within Pf1N1B4_1684 at 1931.491 kb on + strand, within Pf1N1B4_1684 at 1931.502 kb on - strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.510 kb on - strand, within Pf1N1B4_1684 at 1931.510 kb on - strand, within Pf1N1B4_1684 at 1931.510 kb on - strand, within Pf1N1B4_1684 at 1931.510 kb on - strand, within Pf1N1B4_1684 at 1931.593 kb on + strand, within Pf1N1B4_1684 at 1931.594 kb on - strand, within Pf1N1B4_1684 at 1931.604 kb on + strand, within Pf1N1B4_1684 at 1931.604 kb on + strand, within Pf1N1B4_1684 at 1931.605 kb on - strand, within Pf1N1B4_1684 at 1931.605 kb on - strand, within Pf1N1B4_1684 at 1931.605 kb on - strand, within Pf1N1B4_1684 at 1931.605 kb on - strand, within Pf1N1B4_1684 at 1931.608 kb on + strand, within Pf1N1B4_1684 at 1931.628 kb on + strand, within Pf1N1B4_1684 at 1931.628 kb on + strand, within Pf1N1B4_1684 at 1931.628 kb on + strand, within Pf1N1B4_1684 at 1931.629 kb on - strand, within Pf1N1B4_1684 at 1931.629 kb on - strand, within Pf1N1B4_1684 at 1931.629 kb on - strand, within Pf1N1B4_1684 at 1931.656 kb on + strand, within Pf1N1B4_1684 at 1931.656 kb on + strand, within Pf1N1B4_1684 at 1931.656 kb on + strand, within Pf1N1B4_1684 at 1931.656 kb on + strand, within Pf1N1B4_1684 at 1931.657 kb on - strand, within Pf1N1B4_1684 at 1931.657 kb on - strand, within Pf1N1B4_1684 at 1931.657 kb on - strand, within Pf1N1B4_1684 at 1931.657 kb on - strand, within Pf1N1B4_1684 at 1931.807 kb on - strand, within Pf1N1B4_1684 at 1931.807 kb on - strand, within Pf1N1B4_1684
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 1,929,051 + Pf1N1B4_1681 0.83 -2.5 1,929,174 + Pf1N1B4_1681 0.88 -0.6 1,929,174 + Pf1N1B4_1681 0.88 -0.8 1,929,174 + Pf1N1B4_1681 0.88 +2.4 1,929,174 + Pf1N1B4_1681 0.88 +0.2 1,929,174 + Pf1N1B4_1681 0.88 +0.7 1,929,174 + +0.4 1,929,175 - Pf1N1B4_1681 0.88 -0.9 1,929,175 - Pf1N1B4_1681 0.88 -0.6 1,929,175 - Pf1N1B4_1681 0.88 +0.3 1,929,175 - -0.2 1,929,334 + -0.0 1,929,574 + Pf1N1B4_1682 0.29 -1.1 1,929,574 + Pf1N1B4_1682 0.29 -0.5 1,929,574 + -0.2 1,929,574 + Pf1N1B4_1682 0.29 +0.6 1,929,574 + Pf1N1B4_1682 0.29 +1.4 1,929,574 + Pf1N1B4_1682 0.29 +1.4 1,929,574 + Pf1N1B4_1682 0.29 -3.5 1,929,575 - Pf1N1B4_1682 0.29 +0.4 1,929,575 - Pf1N1B4_1682 0.29 -0.8 1,929,575 - Pf1N1B4_1682 0.29 -0.5 1,929,575 - +3.7 1,929,575 - Pf1N1B4_1682 0.29 +2.0 1,929,575 - Pf1N1B4_1682 0.29 -0.4 1,929,575 - Pf1N1B4_1682 0.29 -0.4 1,929,575 - Pf1N1B4_1682 0.29 +0.4 1,929,639 + +2.0 1,929,639 + Pf1N1B4_1682 0.42 -1.9 1,929,639 + Pf1N1B4_1682 0.42 -0.9 1,929,639 + Pf1N1B4_1682 0.42 -1.4 1,929,639 + Pf1N1B4_1682 0.42 -0.4 1,929,639 + Pf1N1B4_1682 0.42 -0.8 1,929,639 + Pf1N1B4_1682 0.42 -0.9 1,929,639 + Pf1N1B4_1682 0.42 -1.1 1,929,640 - Pf1N1B4_1682 0.42 +1.4 1,929,640 - Pf1N1B4_1682 0.42 -1.3 1,929,714 + Pf1N1B4_1682 0.57 -1.2 1,929,714 + Pf1N1B4_1682 0.57 +0.3 1,929,714 + Pf1N1B4_1682 0.57 -0.7 1,929,715 - Pf1N1B4_1682 0.57 +0.8 1,929,715 - Pf1N1B4_1682 0.57 +1.0 1,929,776 - Pf1N1B4_1682 0.70 +1.0 1,929,852 + Pf1N1B4_1682 0.85 -0.2 1,929,852 + Pf1N1B4_1682 0.85 -0.6 1,929,852 + Pf1N1B4_1682 0.85 -0.1 1,929,853 - Pf1N1B4_1682 0.85 +1.2 1,929,913 - +0.4 1,930,004 + -0.4 1,930,182 + Pf1N1B4_1683 0.22 +0.1 1,930,388 + Pf1N1B4_1683 0.46 -0.0 1,930,389 - Pf1N1B4_1683 0.46 -1.0 1,930,493 + Pf1N1B4_1683 0.59 -0.9 1,930,967 - -2.1 1,930,967 - -0.6 1,931,298 + Pf1N1B4_1684 0.32 -0.5 1,931,299 - Pf1N1B4_1684 0.32 +0.8 1,931,299 - Pf1N1B4_1684 0.32 -0.5 1,931,299 - Pf1N1B4_1684 0.32 -0.9 1,931,299 - Pf1N1B4_1684 0.32 +0.2 1,931,299 - Pf1N1B4_1684 0.32 +1.1 1,931,299 - Pf1N1B4_1684 0.32 -0.6 1,931,299 - Pf1N1B4_1684 0.32 -1.9 1,931,302 + Pf1N1B4_1684 0.32 -1.5 1,931,302 + Pf1N1B4_1684 0.32 -0.9 1,931,302 + Pf1N1B4_1684 0.32 -2.1 1,931,302 + Pf1N1B4_1684 0.32 +0.6 1,931,302 + Pf1N1B4_1684 0.32 +0.6 1,931,303 - Pf1N1B4_1684 0.33 -1.2 1,931,413 + Pf1N1B4_1684 0.43 -1.2 1,931,414 - Pf1N1B4_1684 0.43 -0.7 1,931,414 - Pf1N1B4_1684 0.43 -1.6 1,931,414 - Pf1N1B4_1684 0.43 +1.4 1,931,440 - -0.9 1,931,440 - Pf1N1B4_1684 0.46 +1.4 1,931,440 - Pf1N1B4_1684 0.46 -2.4 1,931,491 + Pf1N1B4_1684 0.51 -0.2 1,931,502 - Pf1N1B4_1684 0.52 +1.8 1,931,509 + Pf1N1B4_1684 0.52 -1.4 1,931,509 + Pf1N1B4_1684 0.52 +0.4 1,931,509 + Pf1N1B4_1684 0.52 -1.2 1,931,509 + Pf1N1B4_1684 0.52 -0.7 1,931,509 + Pf1N1B4_1684 0.52 -0.7 1,931,509 + Pf1N1B4_1684 0.52 -0.5 1,931,509 + Pf1N1B4_1684 0.52 -0.4 1,931,509 + Pf1N1B4_1684 0.52 -0.9 1,931,510 - Pf1N1B4_1684 0.53 -1.6 1,931,510 - Pf1N1B4_1684 0.53 -0.6 1,931,510 - Pf1N1B4_1684 0.53 +0.8 1,931,510 - Pf1N1B4_1684 0.53 -1.6 1,931,593 + Pf1N1B4_1684 0.61 +1.0 1,931,594 - Pf1N1B4_1684 0.61 +0.1 1,931,604 + Pf1N1B4_1684 0.62 -0.2 1,931,604 + Pf1N1B4_1684 0.62 +1.4 1,931,605 - Pf1N1B4_1684 0.62 +1.0 1,931,605 - Pf1N1B4_1684 0.62 +1.9 1,931,605 - Pf1N1B4_1684 0.62 +0.1 1,931,605 - Pf1N1B4_1684 0.62 -1.2 1,931,608 + Pf1N1B4_1684 0.62 +0.3 1,931,628 + Pf1N1B4_1684 0.64 -0.9 1,931,628 + Pf1N1B4_1684 0.64 -0.2 1,931,628 + Pf1N1B4_1684 0.64 -0.9 1,931,629 - Pf1N1B4_1684 0.64 +0.0 1,931,629 - Pf1N1B4_1684 0.64 -1.2 1,931,629 - Pf1N1B4_1684 0.64 -1.4 1,931,656 + Pf1N1B4_1684 0.67 -1.9 1,931,656 + Pf1N1B4_1684 0.67 +1.7 1,931,656 + Pf1N1B4_1684 0.67 -0.0 1,931,656 + Pf1N1B4_1684 0.67 +0.2 1,931,657 - Pf1N1B4_1684 0.67 +0.4 1,931,657 - Pf1N1B4_1684 0.67 +0.3 1,931,657 - Pf1N1B4_1684 0.67 +0.1 1,931,657 - Pf1N1B4_1684 0.67 -1.0 1,931,807 - Pf1N1B4_1684 0.81 -0.6 1,931,807 - Pf1N1B4_1684 0.81 +1.4
Or see this region's nucleotide sequence