Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1675 and Pf1N1B4_1676 are separated by 250 nucleotides Pf1N1B4_1676 and Pf1N1B4_1677 are separated by 26 nucleotides
Pf1N1B4_1675 - Transcriptional regulator, at 1,919,574 to 1,920,494
_1675
Pf1N1B4_1676 - Inner membrane component of tripartite multidrug resistance system, at 1,920,745 to 1,922,283
_1676
Pf1N1B4_1677 - Membrane fusion component of tripartite multidrug resistance system, at 1,922,310 to 1,923,374
_1677
Position (kb)
1920
1921
1922
1923 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1919.911 kb on + strand, within Pf1N1B4_1675 at 1919.911 kb on + strand, within Pf1N1B4_1675 at 1919.911 kb on + strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1920.539 kb on + strand at 1920.539 kb on + strand at 1920.539 kb on + strand at 1920.540 kb on - strand at 1920.551 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.665 kb on + strand at 1920.665 kb on + strand at 1920.666 kb on - strand at 1920.666 kb on - strand at 1920.666 kb on - strand at 1920.666 kb on - strand at 1920.676 kb on + strand at 1920.708 kb on - strand at 1920.743 kb on + strand at 1920.852 kb on - strand at 1920.964 kb on + strand, within Pf1N1B4_1676 at 1920.964 kb on + strand, within Pf1N1B4_1676 at 1920.965 kb on - strand, within Pf1N1B4_1676 at 1920.965 kb on - strand at 1920.965 kb on - strand, within Pf1N1B4_1676 at 1921.042 kb on + strand, within Pf1N1B4_1676 at 1921.096 kb on + strand, within Pf1N1B4_1676 at 1921.097 kb on - strand, within Pf1N1B4_1676 at 1921.204 kb on + strand, within Pf1N1B4_1676 at 1921.204 kb on + strand, within Pf1N1B4_1676 at 1921.205 kb on - strand, within Pf1N1B4_1676 at 1921.205 kb on - strand, within Pf1N1B4_1676 at 1921.282 kb on + strand, within Pf1N1B4_1676 at 1921.282 kb on + strand, within Pf1N1B4_1676 at 1921.282 kb on + strand, within Pf1N1B4_1676 at 1921.283 kb on - strand, within Pf1N1B4_1676 at 1921.676 kb on - strand, within Pf1N1B4_1676 at 1921.892 kb on - strand, within Pf1N1B4_1676 at 1921.892 kb on - strand, within Pf1N1B4_1676 at 1922.195 kb on - strand at 1922.195 kb on - strand at 1922.310 kb on - strand at 1922.379 kb on + strand at 1922.379 kb on + strand at 1922.411 kb on + strand at 1922.411 kb on + strand at 1922.411 kb on + strand at 1922.412 kb on - strand at 1922.451 kb on + strand, within Pf1N1B4_1677 at 1922.451 kb on + strand, within Pf1N1B4_1677 at 1922.451 kb on + strand, within Pf1N1B4_1677 at 1922.560 kb on + strand, within Pf1N1B4_1677 at 1922.561 kb on - strand, within Pf1N1B4_1677 at 1922.931 kb on + strand, within Pf1N1B4_1677 at 1922.932 kb on - strand, within Pf1N1B4_1677 at 1922.932 kb on - strand, within Pf1N1B4_1677 at 1922.932 kb on - strand, within Pf1N1B4_1677 at 1922.943 kb on + strand, within Pf1N1B4_1677 at 1922.943 kb on + strand, within Pf1N1B4_1677 at 1922.943 kb on + strand, within Pf1N1B4_1677 at 1922.943 kb on + strand, within Pf1N1B4_1677 at 1922.943 kb on + strand, within Pf1N1B4_1677 at 1922.944 kb on - strand, within Pf1N1B4_1677 at 1922.945 kb on + strand, within Pf1N1B4_1677 at 1922.946 kb on - strand, within Pf1N1B4_1677 at 1922.946 kb on - strand, within Pf1N1B4_1677 at 1922.946 kb on - strand, within Pf1N1B4_1677 at 1922.946 kb on - strand, within Pf1N1B4_1677 at 1923.006 kb on + strand, within Pf1N1B4_1677 at 1923.006 kb on + strand, within Pf1N1B4_1677 at 1923.006 kb on + strand, within Pf1N1B4_1677 at 1923.006 kb on + strand at 1923.006 kb on + strand, within Pf1N1B4_1677 at 1923.006 kb on + strand, within Pf1N1B4_1677 at 1923.006 kb on + strand, within Pf1N1B4_1677 at 1923.006 kb on + strand, within Pf1N1B4_1677 at 1923.006 kb on + strand, within Pf1N1B4_1677 at 1923.007 kb on - strand, within Pf1N1B4_1677 at 1923.007 kb on - strand, within Pf1N1B4_1677 at 1923.007 kb on - strand, within Pf1N1B4_1677 at 1923.007 kb on - strand, within Pf1N1B4_1677 at 1923.007 kb on - strand, within Pf1N1B4_1677 at 1923.007 kb on - strand, within Pf1N1B4_1677 at 1923.007 kb on - strand, within Pf1N1B4_1677 at 1923.007 kb on - strand, within Pf1N1B4_1677 at 1923.057 kb on + strand, within Pf1N1B4_1677 at 1923.057 kb on + strand, within Pf1N1B4_1677 at 1923.057 kb on + strand, within Pf1N1B4_1677 at 1923.057 kb on + strand, within Pf1N1B4_1677 at 1923.058 kb on - strand, within Pf1N1B4_1677 at 1923.058 kb on - strand, within Pf1N1B4_1677 at 1923.058 kb on - strand, within Pf1N1B4_1677 at 1923.058 kb on - strand, within Pf1N1B4_1677 at 1923.058 kb on - strand, within Pf1N1B4_1677 at 1923.058 kb on - strand, within Pf1N1B4_1677 at 1923.097 kb on + strand, within Pf1N1B4_1677
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 1,919,911 + Pf1N1B4_1675 0.37 +1.0 1,919,911 + Pf1N1B4_1675 0.37 +0.7 1,919,911 + Pf1N1B4_1675 0.37 -0.2 1,919,912 - Pf1N1B4_1675 0.37 +2.4 1,919,912 - Pf1N1B4_1675 0.37 -1.5 1,919,912 - Pf1N1B4_1675 0.37 -1.6 1,919,912 - Pf1N1B4_1675 0.37 -0.2 1,920,539 + -0.2 1,920,539 + +1.6 1,920,539 + +0.1 1,920,540 - -1.2 1,920,551 + -0.6 1,920,663 + -0.6 1,920,663 + -0.9 1,920,663 + -1.2 1,920,663 + -0.9 1,920,663 + -0.1 1,920,663 + +0.2 1,920,663 + +0.7 1,920,663 + +0.1 1,920,663 + -2.1 1,920,663 + -1.6 1,920,663 + -2.3 1,920,664 - +0.8 1,920,664 - +0.1 1,920,664 - -0.4 1,920,664 - -2.3 1,920,664 - -0.4 1,920,664 - +1.4 1,920,665 + -1.4 1,920,665 + +0.2 1,920,666 - -0.6 1,920,666 - +0.2 1,920,666 - +0.2 1,920,666 - -1.6 1,920,676 + +0.6 1,920,708 - -1.8 1,920,743 + -0.4 1,920,852 - -0.4 1,920,964 + Pf1N1B4_1676 0.14 -2.4 1,920,964 + Pf1N1B4_1676 0.14 +0.6 1,920,965 - Pf1N1B4_1676 0.14 -0.2 1,920,965 - -0.9 1,920,965 - Pf1N1B4_1676 0.14 -1.6 1,921,042 + Pf1N1B4_1676 0.19 -0.4 1,921,096 + Pf1N1B4_1676 0.23 -1.6 1,921,097 - Pf1N1B4_1676 0.23 -0.4 1,921,204 + Pf1N1B4_1676 0.30 -1.2 1,921,204 + Pf1N1B4_1676 0.30 +1.4 1,921,205 - Pf1N1B4_1676 0.30 -1.2 1,921,205 - Pf1N1B4_1676 0.30 -0.2 1,921,282 + Pf1N1B4_1676 0.35 +0.8 1,921,282 + Pf1N1B4_1676 0.35 -1.2 1,921,282 + Pf1N1B4_1676 0.35 -1.2 1,921,283 - Pf1N1B4_1676 0.35 -1.2 1,921,676 - Pf1N1B4_1676 0.60 -1.0 1,921,892 - Pf1N1B4_1676 0.75 -0.1 1,921,892 - Pf1N1B4_1676 0.75 +0.1 1,922,195 - -1.2 1,922,195 - +0.8 1,922,310 - +0.6 1,922,379 + -1.0 1,922,379 + -0.8 1,922,411 + +0.7 1,922,411 + -1.4 1,922,411 + +1.1 1,922,412 - +0.4 1,922,451 + Pf1N1B4_1677 0.13 +0.4 1,922,451 + Pf1N1B4_1677 0.13 +0.4 1,922,451 + Pf1N1B4_1677 0.13 +2.2 1,922,560 + Pf1N1B4_1677 0.23 +0.7 1,922,561 - Pf1N1B4_1677 0.24 -0.2 1,922,931 + Pf1N1B4_1677 0.58 -0.5 1,922,932 - Pf1N1B4_1677 0.58 -1.2 1,922,932 - Pf1N1B4_1677 0.58 +0.1 1,922,932 - Pf1N1B4_1677 0.58 -0.2 1,922,943 + Pf1N1B4_1677 0.59 -0.2 1,922,943 + Pf1N1B4_1677 0.59 -0.2 1,922,943 + Pf1N1B4_1677 0.59 -0.6 1,922,943 + Pf1N1B4_1677 0.59 +1.4 1,922,943 + Pf1N1B4_1677 0.59 -0.6 1,922,944 - Pf1N1B4_1677 0.60 -0.6 1,922,945 + Pf1N1B4_1677 0.60 +0.1 1,922,946 - Pf1N1B4_1677 0.60 +0.2 1,922,946 - Pf1N1B4_1677 0.60 +0.4 1,922,946 - Pf1N1B4_1677 0.60 -0.2 1,922,946 - Pf1N1B4_1677 0.60 +0.7 1,923,006 + Pf1N1B4_1677 0.65 -0.4 1,923,006 + Pf1N1B4_1677 0.65 +0.1 1,923,006 + Pf1N1B4_1677 0.65 -0.8 1,923,006 + +0.4 1,923,006 + Pf1N1B4_1677 0.65 +0.3 1,923,006 + Pf1N1B4_1677 0.65 +1.7 1,923,006 + Pf1N1B4_1677 0.65 -1.2 1,923,006 + Pf1N1B4_1677 0.65 +1.6 1,923,006 + Pf1N1B4_1677 0.65 -2.4 1,923,007 - Pf1N1B4_1677 0.65 +0.2 1,923,007 - Pf1N1B4_1677 0.65 -0.9 1,923,007 - Pf1N1B4_1677 0.65 +1.2 1,923,007 - Pf1N1B4_1677 0.65 -0.8 1,923,007 - Pf1N1B4_1677 0.65 +0.2 1,923,007 - Pf1N1B4_1677 0.65 -0.2 1,923,007 - Pf1N1B4_1677 0.65 -0.1 1,923,007 - Pf1N1B4_1677 0.65 -0.7 1,923,057 + Pf1N1B4_1677 0.70 +0.7 1,923,057 + Pf1N1B4_1677 0.70 -1.8 1,923,057 + Pf1N1B4_1677 0.70 -0.4 1,923,057 + Pf1N1B4_1677 0.70 +0.4 1,923,058 - Pf1N1B4_1677 0.70 -2.1 1,923,058 - Pf1N1B4_1677 0.70 +1.4 1,923,058 - Pf1N1B4_1677 0.70 +0.4 1,923,058 - Pf1N1B4_1677 0.70 -0.4 1,923,058 - Pf1N1B4_1677 0.70 -0.2 1,923,058 - Pf1N1B4_1677 0.70 +0.7 1,923,097 + Pf1N1B4_1677 0.74 +0.9
Or see this region's nucleotide sequence